1. Recent advances in the functional characterization of honeybee voltage-gated Ca2+ channels
- Author
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M. Chahine, J-B. Thibaud, M. Rousset, C. Collet, Thierry Cens, Pierre Charnet, Claudine Menard, Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] (IBMM), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM), Université de Montpellier (UM), Abeilles & Environnement (UR 406 ), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université Laval [Québec] (ULaval), Centre National de la Recherche Scientifique (CNRS), Institut National de la Sante et de la Recherche Medicale (INSERM), University of Monpellier, Agence Nationale de la Recherche (ANR Bee-Chennels) [ANR-13-BSV7-0010-0], Fondation Lune et Miel, Institut des Biomolecules Max Mousseron [UMR 5247], and NSERC Discovery Grant from the Natural Science and Engineering Research Council of Canada
- Subjects
0303 health sciences ,Voltage-gated ion channel ,Chemistry ,business.industry ,[SDV]Life Sciences [q-bio] ,Characterization (materials science) ,03 medical and health sciences ,0302 clinical medicine ,[SDE]Environmental Sciences ,Optoelectronics ,Ca2 channels ,business ,030217 neurology & neurosurgery ,030304 developmental biology - Abstract
Symposium on Advances in Agrochemicals: Ion Channels and G Protein-Coupled Receptors (GPCRs) as Targets for Pest Control / 252nd ACS National Meeting and Exposition, 21-25 aôut 2016 Philadelphia, PA – États-Unis.; International audience; Voltage-gated Ca2+ (CaV) channels allow Ca2+ to enter the cell in response to membrane depolarization. This Ca2+ influx is not only necessary for cell excitability, but also triggers, via Ca2+-binding proteins, important biological functions such as contraction, synaptic transmission, or gene expression. Insect CaV channels are encoded by only three genes (against 10 in mammals), and their invalidation or pharmacological blockade is expected to have deleterious effects. They may thus constitute interesting targets for specific insecticides. However, the precise identification of the genes underlying the different Ca2+ currents recorded in different tissues, as well as the heterologous expression of these genes to screen selective molecules, have been proven to be difficult. This chapter reports on the recent successful expression of honeybee Ca2+ channels genes in Xenopus oocytes and reviews pharmacological properties of Ca2+ currents recorded in isolated honeybee neurons and muscle cells.
- Published
- 2017
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