Search

Resume Au cours d'une etude systematique de l'immunisation, les auteurs ont montre que la meilleure voie d'introduction des levures etait la voie intraveineuse et que les levures vivantes ou tuees ainsi que les parois possedaient sensiblement le měme pouvoir antigenique. En employant des serums monospecifiques et comme techniques serologiques l'immunofluorescence et l'agglutination, ils ont pu mettre en evidence l'existence de parentes antigeniques etroites entre sept souhes de levures. Summary Through a systematic study of immunization the authors have shown that the best way to introduce yeasts was intravenous and that yeasts alive or destroyed and walls had more or less the same antigenic power. Using monospecific sera and as serological technics immunofluorescence and agglutination the authors have been able to bring to light close antigenic relationship between seven yeasts. Zusammenfassung Aufgrund einer systematischen Immunisierungsstudie haben die Autoren gezeigt, datβ der beste Weg der Inokulation von Hefen der intravenose war und daβ sowohl die lebenden und die toten Hefen als auch die Zellwande ungefahr die selbe Fahigkeit besitzen, Antigene zu bilden. Bei den Verwendung monospezifischer Sera und als serologische Tehniken die Immunofluoreszenz und die Agglutination haben die Autoren die Existenz enger Antigengemein-schaften zwischen sieben Hefestammen nahgewiesen.

Nonspecific protection against infectious aerosols of influenza A virus was obtained in Swiss mice after vaccination by aerosols of bacterial ribosomes together with membranal glycoproteins extracted from Klebsiella pneumoniae as the adjuvant. It was shown that repeated stimulant aerosols were necessary to obtain this protection. Routine estimation of serum interferon levels after administration of the association of ribosomes plus membranal glycoproteins to the animals by aerosol or intravenous route showed that there was no correlation between protection and the presence of serum interferon. It was shown that the serum interferon-inducer activity was due to ribosomes. No induction of serum interferon was obtained with membranal glycoproteins used separately. Local liberation of interferon in the mucous membrane of the upper respiratory tract was not investigated.

Resume Le virus de l'hepatite A (VHA) a ete concentre, a partir d'eaux experimentalement contaminees, par la methode d'adsorption/elution sur membrane d'esters de cellulose, suivie d'une concentration de l'eluat par le polyethylene glycol 6000. Le VHA est detecte a chaque etape par radioimmunologie. L'efficacite d'adsorption est superieure a 90%, aux pH compris entre 3 et 4. Pour l'elution avec l'extrait de boeuf a 3%, de meme que pour le tampon glycine 0,2 M, les differences observees en fonction du pH (de 7,5 a 11,5) et du nombre de passages de l'eluant a travers le filtre (1 ou 3) ne sont pas statistiquement significatives. L'extrait de boeuf a 3% a donne de meilleurs resultats que le tampon glycine 0.2 M. D'autre part, le VHA s'est multiplie en culture cellulaire apres concentration par ce procede.

13 strains of imperfect fungi have been studied in Lyon according to a biochemical criteria or growth of the studied species in a minimum but nitrogenous medium containing carbon elements. The inoculation is achieved by Api System ensemencement. The germination of the spores showing the carbon assimilation involves the appearance of a trouble. The intensity of this growth is coded by + or −.

White rats were thymectomized neonatally. After 60 days, no alteration was noted in the plasma or testis magnesium level. On the other hand, a marked decrease was observed in the magnesium content of muscle (−23%) and of bone (−19%). The injection of thymosin reversed these changes. These findings suggest that a correlation between the thymus and the muscle is exerted by a protein factor (thymosin), and that the thymus may act on sex glands through an indirect pathway.

One of the earlier effects of poliovirus was the maintaining of a high level of Hela cells 5'-nucleotidase activity (maximal efficiency for a virus/cell ratio = 500 ID 50/cell) (ID50:50% Infections Dose). The activity decreased when cells were suspended in the presence of EDTA(EDTA:Ethylene Diamine Tetracetic Acid) and was partially restored by adding 10 mM Mg++ interactions are discussed.One of the earlier effects of poliovirus was the maintaining of a high level of Hela cells 5'-nucleotidase activity (maximal efficiency for a virus/cell ratio = 500 ID 50/cell) (ID50:50% Infections Dose). The activity decreased when cells were suspended in the presence of EDTA(EDTA:Ethylene Diamine Tetracetic Acid) and was partially restored by adding 10 mM Mg++ interactions are discussed.

5'-nucleotidase activity of cultured diploid cells was measured using intact suspended cells. This depended on the substrate amount, but did not vary with Mg ++ concentration-Kinetic data were determined using Con. A,a specific inhibitor.5'-nucleotidase activity of cultured diploid cells was measured using intact suspended cells. This depended on the substrate amount, but did not vary with Mg ++ concentration-Kinetic data were determined using Con. A,a specific inhibitor.

The two pathogenic species Streptococcus pneumoniae and Klebsiella pneumoniae were used to analyze the immunogenic role of proteins in ribosomal preparations. The protective activity of ribosomes prepared from either strain and further purified by washing with high-salt concentrations, followed or not by sucrose gradient separation of the particles, was identical to that of crude unwashed ribosomes. Similarly, no substantial alteration of the level of protection was observed after treatment with the antibiotic puromycin. Therefore, the immunizing efficacy of ribosomes does not appear to be due either to the nonribosomal proteins adsorbed at the surface of organelles or to the growing polypeptide chain. It seems rather to be attributable to the structural ribosomal proteins themselves, which were indeed shown to induce alone a significant level of protection.The two pathogenic species Streptococcus pneumoniae and Klebsiella pneumoniae were used to analyze the immunogenic role of proteins in ribosomal preparations. The protective activity of ribosomes prepared from either strain and further purified by washing with high-salt concentrations, followed or not by sucrose gradient separation of the particles, was identical to that of crude unwashed ribosomes. Similarly, no substantial alteration of the level of protection was observed after treatment with the antibiotic puromycin. Therefore, the immunizing efficacy of ribosomes does not appear to be due either to the nonribosomal proteins adsorbed at the surface of organelles or to the growing polypeptide chain. It seems rather to be attributable to the structural ribosomal proteins themselves, which were indeed shown to induce alone a significant level of protection.

Male Balb/c mice were exposed in an anechoic chamber to 2450 MHz CW microwaves at 5, 10, 15, and 20 mW/cm2, 4 hours daily for 4 days, under controlled environmental conditions. T-cell cytotoxicity and natural killer (NK) cell activity were compared in exposed and sham animals. A significant decrease (p less than 0.001) of cell-mediated cytotoxicity was observed only for a power density of 20 mW/cm2. A significant increase (p less than 0.01) in NK activity was demonstrated following exposures at 15 mW/cm2.