1. Sympathetic innervation of interscapular brown adipose tissue is not a predominant mediator of oxytocin-elicited reductions of body weight and adiposity in male diet-induced obese mice
- Author
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Edwards, Melise M, Nguyen, Ha K, Dodson, Andrew D, Herbertson, Adam J, Wolden-Hanson, Tami, Wietecha, Tomasz A, Honeycutt, Mackenzie K, Slattery, Jared D, O’Brien, Kevin D, Graham, James L, Havel, Peter J, Mundinger, Thomas O, Sikkema, Carl L, Peskind, Elaine R, Ryu, Vitaly, Taborsky, Gerald J, and Blevins, James E
- Subjects
Medical Physiology ,Biomedical and Clinical Sciences ,Obesity ,Neurosciences ,Nutrition ,Metabolic and endocrine ,Animals ,Oxytocin ,Adipose Tissue ,Brown ,Male ,Mice ,Sympathetic Nervous System ,Diet ,High-Fat ,Adiposity ,Mice ,Inbred C57BL ,Body Weight ,Weight Loss ,Mice ,Obese ,Energy Metabolism ,Norepinephrine ,obesity ,brown adipose tissue ,white adipose tissue ,oxytocin ,food intake ,Clinical Sciences ,Nutrition and Dietetics ,Clinical sciences - Abstract
Previous studies indicate that CNS administration of oxytocin (OT) reduces body weight in high fat diet-induced obese (DIO) rodents by reducing food intake and increasing energy expenditure (EE). We recently demonstrated that hindbrain (fourth ventricular [4V]) administration of OT elicits weight loss and elevates interscapular brown adipose tissue temperature (TIBAT, a surrogate measure of increased EE) in DIO mice. What remains unclear is whether OT-elicited weight loss requires increased sympathetic nervous system (SNS) outflow to IBAT. We hypothesized that OT-induced stimulation of SNS outflow to IBAT contributes to its ability to activate BAT and elicit weight loss in DIO mice. To test this hypothesis, we determined the effect of disrupting SNS activation of IBAT on the ability of 4V OT administration to increase TIBAT and elicit weight loss in DIO mice. We first determined whether bilateral surgical SNS denervation to IBAT was successful as noted by ≥ 60% reduction in IBAT norepinephrine (NE) content in DIO mice. NE content was selectively reduced in IBAT at 1-, 6- and 7-weeks post-denervation by 95.9 ± 2.0, 77.4 ± 12.7 and 93.6 ± 4.6% (P
- Published
- 2024