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Background: Skeletal muscle is a plastic tissue that adapts to increased mechanical loading/contractile activity through fusion of muscle stem cells (MuSCs) with myofibers, a physiological process referred to as myonuclear accretion. However, it is still unclear whether myonuclear accretion is driven by increased mechanical loading per se, or occurs, at least in part, in response to muscle injury/regeneration. Here, we developed a non-damaging protocol to evaluate contractile activity-induced myonuclear accretion/hypertrophy in physiological conditions. Methods: Contractile activity was generated by applying repeated electrical stimuli over the mouse plantar flexor muscles. This method is commonly referred to as NeuroMuscular Electrical Simulation (NMES) in Human. Each NMES training session consisted of 80 isometric contractions delivered at ∼15% of maximal tetanic force to avoid muscle damage. C57BL/6J male mice were submitted to either a short (i.e., 6 sessions) or long (i.e., 12 sessions) individualized NMES training program while unstimulated mice were used as controls. Histological investigations were performed to assess the impact of NMES on MuSC number and status, myonuclei content and muscle tissue integrity, typology and size. Results: NMES led to a robust proliferation of MuSCs and myonuclear accretion in the absence of overt signs of muscle damage/regeneration. NMES-induced myonuclear accretion was specific to type IIB myofibers and was an early event preceding muscle hypertrophy inasmuch as a mild increase in myofiber cross-sectional area was only observed in response to the long-term NMES training protocol. Conclusion: We conclude that NMES-induced myonuclear accretion and muscle hypertrophy are driven by a mild increase in mechanical loading in the absence of overt signs of muscle injury. [ABSTRACT FROM AUTHOR]

Accurate control of force on the environment is mechanically necessary for many tasks involving the lower extremities. We investigated drifts in the horizontal (shear) active force produced by right-footed seated subjects and the effects of force matching by the other foot. Subjects generated constant shear force at 15% and 30% of maximal voluntary contraction (MVC) using one foot. Visual feedback of shear force magnitude was provided for the first 5s, then turned off for 30s. During the 30% MVC task, we observed parallel drops in active shear and vertical force magnitudes leading to consistent drifts in the resultant force magnitude, not in its direction. Force matching by the other foot resulted in significantly lower forces when feedback was available throughout the trial. No feedback was provided for the matching foot. When the matching foot began exerting force, the task foot experienced a notable drop in all force components, with a change in force direction only for the task foot. After this initial drop, the downward drift in the task foot stopped or reversed. Subjects were unaware of these drifts and errors. Our findings suggest that shear force production involves setting a referent coordinate vector, which shows drifts and matching errors, while its direction remains stable. Involvement of the matching foot appears to perturb the neural commands to the task foot, with minor differences observed between feet. The discrepancy between the consistent force drifts and lack of awareness of the drifts indicates a difference between force perception-to-act and perception-to-report. [ABSTRACT FROM AUTHOR]

Purpose: To determine whether there are bilateral and interdigit differences in the maximal force production of experienced climbers and whether these differences are mediated by ability level or preferred style of climbing. Methods: Thirty-six male climbers (age 30 [9.4] y) took part in a single-session trial to test their maximal force production on both hands. The tests included a one-arm maximal isometric finger flexor strength test (MIFS) and a one-arm individual MIFS. Bilateral differences were analyzed by strongest hand (defined as the hand that produced the highest MIFS value) and dominance (defined as the writing hand). Results: A pairwise t test found that MIFS was significantly greater for the strongest hand (mean difference = 4.1%, 95% CI, −0.052 to 0.029, P <.001), with handedness explaining 89% of the variation. A 2-way mixed-model analysis of variance determined that there were no interactions between preferred style (bouldering or sport climbing) and MIFS or between ability level (advanced or elite) and MIFS. Conclusions: Climbers have significant finger flexor strength bilateral asymmetries between their strongest and weakest hand. Moreover, when dominance is controlled, this difference in strength is present, with the dominant hand producing more force. Neither preferred style of climbing nor the ability level of the climbers could explain these asymmetries. As such, practitioners should consider regularly monitoring unilateral strength, aiming to minimize the likelihood of large bilateral asymmetry occurring. [ABSTRACT FROM AUTHOR]

ABSTRACT Introduction and Aims Methods Results Discussion Mitochondrial myopathies are rare genetic disorders for which no effective treatment exists. We previously showed that the pharmacological cyclophilin inhibitor cyclosporine A (CsA) extends the lifespan of fast‐twitch skeletal muscle‐specific mitochondrial transcription factor A knockout (Tfam KO) mice, lacking the ability to transcribe mitochondrial DNA and displaying lethal mitochondrial myopathy. Our present aim was to assess whether the positive effect of CsA was associated with improved in vivo mitochondrial energy production.Mice were treated with CsA for 4 weeks, beginning at 12 weeks (i.e., before the terminal disease phase). Hindlimb plantar flexor muscles were fatigued by 80 contractions (40 Hz, 1.5 s on, 6 s off) while measuring force and energy metabolism using phosphorus‐31 magnetic resonance spectroscopy.Force decreased at similar rates in Tfam KO mice with and without the CsA treatment, reaching 50% of the baseline value after ~14 ± 1 contractions, which was faster than in control mice (25 ± 1 contractions). Phosphocreatine (PCr) decreased to ~10% of the control concentration in Tfam KO mice, independent of the treatment, which was larger than the ~20% observed in control mice. The time constant of PCr recovery was higher in untreated Tfam KO than that in control muscle (+100%) and similar in untreated and CsA‐treated Tfam KO mice.The results do not support improved mitochondrial energy production as a mechanism underlying the prolonged lifespan of Tfam KO mitochondrial myopathy mice treated with CsA. Thus, other mechanisms must be involved, such as the previously observed CsA‐mediated protection against excessive mitochondrial Ca2+ accumulation. [ABSTRACT FROM AUTHOR]

This study explored the effects of strength-focused, speed-focused, and non-specific resistance training on the athletic performance and sprint biomechanics through force-velocity-power (FVP) profile in youth professional soccer players (age: 17.6 ± 0.9 years). In a 6-week randomized-controlled trial, 24 male participants were assigned to strength, speed, or control groups (n = 8 each). We assessed sprint performance, including sprint split times and the sprint FVP profile. Post-training, all groups showed significant enhancements in sprint times (p = 0.000-0.004, η² = 0.32-0.75) and FVP profile variables (p = 0.000-0.049; η² = 0.17-0.73). The strength group exhibited notable improvements in the maximal ratio of horizontal-to-resultant force (p = 0.026, d = 0.78) and maximal power (Phmax) (p = 0.013, d = 0.89) compared to controls. However, maximum velocity and maximum velocity at the end of acceleration phase did not significantly change in any group. These findings demonstrate that both strength and speed training significantly enhance force production capabilities in youth soccer players, influencing key FVP profile characteristics, without substantially affecting maximum velocity.

Purpose: Evaluate the effects of 6 weeks of specific-joint isometric strength training on serve velocity (SV), serve accuracy (SA), and force–time curve variables. Methods: Sixteen young competition tennis players were divided into an intervention (n = 10) or control group (n = 6). SV, SA, maximal voluntary isometric contraction, peak rate of force development, rate of force development, and impulse (IMP) at different time frames while performing a shoulder internal rotation (SHIR) or flexion were tested at weeks 0, 3, and 6. Results: The intervention group showed significant increases in SV from pretest to posttest (7.0%, effect size [ES] = 0.87) and no variations in SA. Moreover, the intervention group showed significant increases from pretest to posttest in shoulder-flexion rate of force development at 150 (30.4%, ES = 2.44), 200 (36.5%, ES = 1.26), and 250 ms (43.7%, ES = 1.67) and in SHIR IMP at 150 (35.7%, ES = 1.18), 200 (33.4%, ES = 1.19), and 250 ms (35.6%, ES = 1.08). Furthermore, significant increases were found in shoulder-flexion rate of force development from intertest to posttest at 150 ms (24.5%, ES = 1.07) and in SHIR IMP at 150 (13.5%, ES = 0.90), 200 (19.1%, ES = 0.98), and 250 ms (27.2%, ES = 1.16). SHIR IMP changes from pretest to intertest were found at 150 ms (25.6%, ES = 1.04). The control group did not show changes in any of the tested variables. Conclusions: Six weeks of upper-limb specific-joint isometric strength training alongside habitual technical–tactical workouts results in significant increases in SV without SA detriment in young tennis players. [ABSTRACT FROM AUTHOR]

We explored two types of anticipatory synergy adjustments (ASA) during accurate four-finger total force production task. The first type is a change in the index of force-stabilizing synergy during a steady state when a person is expecting a signal to produce a quick force change, which is seen even when the signal does not come (steady-state ASA). The other type is the drop in in the synergy index prior to a planned force change starting at a known time (transient ASA). The subjects performed a task of steady force production at 10% of maximal voluntary contraction (MVC) followed by a ramp to 20% MVC over 1 s, 3 s, and as a step function (0 s). In another task, in 50% of the trials during the steady-state phase, an unexpected signal could come requiring a quick force pulse to 20% MVC (0-surprise). Inter-trial variance in the finger force space was used to quantify the index of force-stabilizing synergy within the uncontrolled manifold hypothesis. We observed significantly lower synergy index values during the steady state in the 0-ramp trials compared to the 1-ramp and 3-ramp trials. There was also larger transient ASA during the 0-ramp trials. In the 0-surprise condition, the synergy index was significantly higher compared to the 0-ramp condition whereas the transient ASA was significantly larger. The finding of transient ASA scaling is of importance for clinical studies, which commonly involve populations with slower actions, which can by itself be associated with smaller ASAs. The participants varied the sharing pattern of total force across the fingers more in the task with "surprises". This was coupled to more attention to precision of performance, i.e., inter-trial deviations from the target as reflected in smaller variance affecting total force, possibly reflecting higher concentration on the task, which the participants perceived as more challenging compared to a similar task without surprise targets. [ABSTRACT FROM AUTHOR]

Rehabilitation from musculoskeletal injuries focuses on reestablishing and monitoring muscle activation patterns to accurately produce force. The aim of this study is to explore the use of a novel low-powered wearable distributed Simultaneous Musculoskeletal Assessment with Real-Time Ultrasound (SMART-US) device to predict force during an isometric squat task. Participants (N = 5) performed maximum isometric squats under two medical imaging techniques; clinical musculoskeletal motion mode (m-mode) ultrasound on the dominant vastus lateralis and SMART-US sensors placed on the rectus femoris, vastus lateralis, medial hamstring, and vastus medialis. Ultrasound features were extracted, and a linear ridge regression model was used to predict ground reaction force. The performance of ultrasound features to predict measured force was tested using either the Clinical M-mode, SMART-US sensors on the vastus lateralis (SMART-US: VL), rectus femoris (SMART-US: RF), medial hamstring (SMART-US: MH), and vastus medialis (SMART-US: VMO) or utilized all four SMART-US sensors (Distributed SMART-US). Model training showed that the Clinical M-mode and the Distributed SMART-US model were both significantly different from the SMART-US: VL, SMART-US: MH, SMART-US: RF, and SMART-US: VMO models (p < 0.05). Model validation showed that the Distributed SMART-US model had an R2 of 0.80 ± 0.04 and was significantly different from SMART-US: VL but not from the Clinical M-mode model. In conclusion, a novel wearable distributed SMART-US system can predict ground reaction force using machine learning, demonstrating the feasibility of wearable ultrasound imaging for ground reaction force estimation. [ABSTRACT FROM AUTHOR]

Although there are studies showing that myofascial release will increase muscle force production, the contribution of its application alone to muscle force production has not been examined. Aim of the study is to investigate the effect of instrument-assisted soft tissue mobilization (IASTM) on eccentric strength, frontal plane projection angle (FPPA), dynamic (DPS), and static postural stability (SPS), femoral internal rotation (FIR) angle in females with dynamic knee valgus (DKV). A total of 44 recreationally active females with asymptomatic DKV (age: 21,39 ± 1,79, body mass index: 20,09 ± 2,45) participated and were randomly assigned to either control group (CG) or IASTM group (IASTMG). Participants' eccentric contraction strength, FPPA, DPS, SPS, and FIR on the involved leg were measured pre- and post. IASTM application was applied to IASTMG for 6 weeks, twice a week, for 5 min, using Graston Technique® instruments on gluteus medius. CG received no intervention. In comparison of ECS difference values, change in IASTMG was found to be statistically significantly higher than CG (p =.004; p <.01). There was no statistical difference in comparison of FIR and FPPA values (respectively p =.213, p =.360; p <.05). In SPS and DPS evaluation, a statistically significant improvement was observed in favor of IASTMG in comparison of both intergroup and difference values (p <.05 for all). Strength gain without exercise can increase postural stability, but it isn't sufficient to correct faulty movement patterns. We recommend adding IASTM to injury prevention programs, but there is a need to investigate the effect of IASTM with technique correction feedback. [ABSTRACT FROM AUTHOR]

Purpose: To determine the relative contributions of handgrip and individual finger strength for the prediction of climbing performance in a bouldering competition. A secondary aim was to examine the influence of body size, bouldering experience, and training habits. Methods: Sixty-seven boulderers (mean [SD], age = 21.1 [4.0] y; body mass = 69.5 [9.8] kg) volunteered for this study. Data collection occurred immediately before an indoor bouldering competition and involved the assessment of handgrip and individual finger maximal force production using an electronic handheld dynamometer. The bouldering competition consisted of 70 routes graded V0 to V8, with higher point values awarded for completing more difficult routes. Stepwise multiple regression analysis was used to examine the relative contributions of handgrip and individual finger strengths, body mass, height, bouldering experience, and bouldering frequency to the prediction of performance scores in the competition. Results: Ring finger pinch strength, bouldering experience, and bouldering frequency significantly (P <.05) contributed to the model (R2 =.373), whereas body mass; height; full handgrip strength, as well as index, middle, and little finger pinch strengths did not. The β weights showed that ring finger pinch strength (β =.430) was the most significant contributor, followed by bouldering experience (β =.331) and bouldering frequency (β =.244). Conclusions: The current findings indicated that trainable factors contributed to the prediction of bouldering performance. These results suggest greater bouldering frequency and experience likely contribute to greater isolated individual finger strength, thereby optimizing preparation for the diverse handholds in competitive rock climbing. [ABSTRACT FROM AUTHOR]

Purpose: Reduced spinal excitability during the transcranial magnetic stimulation (TMS) silent period (SP) has recently been shown to last longer than previously thought in the upper limbs, as assessed via spinal electrical stimulation. Further, there is reason to expect that contraction intensity affects the duration of the reduced spinal excitability. Methods: This study investigated spinal excitability at different time delays within the TMS-evoked SP in m.rectus femoris. Fifteen participants performed non-fatiguing isometric knee extensions at 25%, 50% and 75% of maximum voluntary contraction (MVC). Lumbar stimulation (LS) induced a lumbar-evoked potential (LEP) of 50% resting M-max. TMS stimulator output induced a SP lasting ~ 200 ms. In each contraction, a LEP (unconditioned) was delivered ~ 2–3 s prior to TMS, which was followed by a second LEP (conditioned) 60, 90, 120 or 150 ms into the silent period. Five contractions were performed at each contraction intensity and for each time delay in random order. Results: Compared to the unconditioned LEP, the conditioned LEP amplitude was reduced (− 28 ± 34%, p = 0.007) only at 60 ms during 25% of MVC. Conditioned LEP amplitudes during 50% and 75% of MVC were reduced at 60 ms (− 37 ± 47%, p = 0.009 and − 37 ± 42%, p = 0.005, respectively) and 150 ms (− 30% ± 37%, p = 0.0083 and − 37 ± 43%, p = 0.005, respectively). LEP amplitude at 90 ms during 50% of MVC also reduced (− 25 ± 35%, p = 0.013). Conclusion: Reduced spinal excitability is extended during 50% and 75% of MVC. In future, paired TMS-LS could be a potential method to understand changes in spinal excitability during SP (at different contraction intensities) when testing various neurophysiological phenomena. [ABSTRACT FROM AUTHOR]

Problem statement: Currently, there is a lack of research exploring the relationship between lower-limb muscle pennation angles and physical performance. In this study, we aimed to investigate the morphological and physiological adaptations in muscle architecture among athletes, potentially influencing performance outcomes. Specifically, there is a need for further investigation into muscle pennation angles (PA) and performance parameters in female athletes. Addressing these gaps is crucial for implementing effective performance enhancement strategies and sport-specific training programmes to optimize sport performance in the female athlete population. Approach: A quantitative study was performed to examine relationships between muscle pennation angles and physical performance. Muscle pennation angles were assessed using ultrasonography in both extended and flexed states. Physical performance data were collected from 22 female athletes participating netball and football. Various performance tests including vertical jump, 40m sprints, Change of Direction (COD) tests and one Repetition Maximum (1RM) tests were conducted. A Shapiro-Wilk test evaluated data distributions. A correlation analyses assessed the strength of linear relationships between PAs and performance parameters, with a significance level of p<0.05. Purpose: While existing literature extensively explores the impact of muscle fascicle length changes during exercise and training protocols in various sports, there is a noticeable lack of attention given to the significance of muscle pennation angles in force production and muscle velocities. This study aims to address this gap by evaluating the relationships between pennation angles and physical performance among netball and football athletes. Additionally, it recognises the interdependence of fascicle length and pennation angles, highlighting their architectural responses to training and sport demands. Results: Correlations presented (p>0.05) small to moderate relationships amongst PAs and performance parameters. Specifically, significant correlations between the left tibialis anterior PA, COD ability t-test (p = 0.006), 1RM strength (p = 0.010) and relative strength (p = 0.009) in the extended state. Correlations were also present between the right tibialis anterior PA, 40m sprint (p = 0.001) and COD ability t-test (p = 0.000) in the extended state. There were significant correlations between right tibialis anterior PA, 10m sprint (p = 0.011), 40m sprint (p = 0.008) and COD ability t-test (p = 0.002) in the flexed state. Conclusion: These findings highlight the significance of muscle architecture adaptations, particularly pennation angles, influencing force production and shortening velocities during athletic activities such as jumping, sprinting and COD actions. This study provides valuable insight into the relationship between muscle architecture and physical performance in female athletes. [ABSTRACT FROM AUTHOR]

Introduction: Latent trigger points (TrPs) are a type of musculoskeletal disorder. That can detrimentally affect athletic performance. Purpose: This study to classify athletes into latent-trigger point (L-TrPs) and non-trigger point (non-TrPs) group base on pain pressure threshold (PPT), force production and range of motion (ROM). Materials and methods: Total 45 L-TrPs and 45 non-TrPs athletes were selected. The diagnostic criteria proposed by Simons were employed to identify L-TrPs. Pressure algometry was used for PPT, HUMAC NORM ISOKINETIC machine for force production and Kinovea software for ROM analysis. Independent t test and discriminant analysis were conducted. Standardized discriminant coefficient, canonical correlation and Wilks' Lambda was calculated and p-value was judges at 5% level of significance. Results: Standardized discriminant coefficient showing that Average PPT hold first position followed by Average Extension ROM followed by Average Flexion ROM while Average force production stands on last position for discriminating ability to classification of the L-TrPs and non-TrPs among the athletes. It also observed that by using PPT, force production, and ROM, the model accurately classifies athletes with both L-TrPs and non-TrPs, with an accuracy ranging from 85% to 93%. This highlights the potential significance of these factors in distinguishing between athletes with and without TrPs. Conclusion: From this statistical analysis it concluded that PPT is the most significant contributor to classify the latent and non-TrPs groups. Therefore, regular pain assessment is required to maintain the performance of an individual. Whereas force production and ROM should not be ignored as the model 85%-95% accurately classifies these groups. [ABSTRACT FROM AUTHOR]

Context: Although functional effects of kinesio taping (KT) have been widely studied, its effects on contractile properties of the target muscle remain unclear. Tensiomyography is suitable for quantifying muscle stiffness and rate of force development upon imposed twitch contraction. Objective: To test the hypothesis that KT has effects on contractile properties of targeted muscle using tensiomyography. Design: Prospective cohort study. Settings: Performance laboratory of a sports rehabilitation center. Participants: A total of 11 healthy volunteers. Interventions: Tensiomyography measurements before KT facilitation technique applied (pre-KT), 45 minutes, and 24 hours after KT (post-KT1 and post-KT2, respectively) without removing the tape. Main Outcome Measures: Maximal radial displacement, contraction time, delay time, sustain time, relaxation time, and velocity of contraction. Results: Significant effects were shown for maximal radial displacement (P = .004), contraction time (P = .013), relaxation time (P = .035), and velocity of contraction (P = .0033), but not for delay time (P = .060) and sustain time (P = .078). Post hoc testing indicated a significant decrease in maximal radial displacement for post-KT1 only (from 6.33 [1.46] to 4.87 [2.14] mm), and a significant increase in contraction time for both post-KT1 and post-KT2 (from 30.87 [11.39] to 39.71 [13.49] ms, and 37.41 [14.73] ms, respectively). Post hoc testing also showed a significant decrease in relaxation time for post-KT2 (from 65.97 [53.43] to 47.45 [38.12] ms), and a significant decrease in velocity of contraction for both post-KT1 and post-KT2 (from 0.22 [0.08] to 0.15 [0.09] mm/s, and 0.16 [0.07] mm/s), respectively. Conclusion: The findings indicate that KT leads to an increased muscle stiffness and a reduced muscle rate of force production despite the facilitation technique applied. [ABSTRACT FROM AUTHOR]

Purpose: (1) To analyze the associations between serve velocity (SV) and various single-joint upper-limb isometric force–time curve parameters, (2) to develop a prediction model based on the relationship between these variables, and (3) to determine whether these factors are capable of discriminating between tennis players with different SV performances. Method: A total of 17 high-performance tennis players performed 8 isometric tests of joints and movements included in the serve kinetic chain (wrist and elbow flexion [EF] and extension; shoulder flexion [SHF] and extension [SHE], internal [SHIR] and external rotation). Isometric force (IF), rate of force development (RFD), and impulse (IMP) at different time intervals (0–250 ms) were obtained for analysis. Results: Significant (P <.05 to P <.01) and moderate to very large correlations were found between SV and isometric force (IF), RFD and impulse (IMP) at different time intervals in all joint positions tested (except for the EF). Stepwise multiple regression analysis highlighted the importance of RFD in the SHIR from 0 to 50 milliseconds and isometric force (IF) in the SHF at 250 milliseconds on SV performance. Moreover, the discriminant analyses established SHIR RFD from 0 to 30 milliseconds as the most important factor discriminating players with different serve performances. Conclusions: Force–time parameters in upper-limb joints involved in the serve moderate to very largely influence SV. Findings suggest that the capability to develop force in short periods of time (<250 ms), especially in the shoulder joint, seems relevant to develop high SV in competition tennis players. [ABSTRACT FROM AUTHOR]

Dysregulation of skeletal muscle morphology and metabolism is associated with chronic diseases such as obesity and type 2 diabetes. The enzyme glycogen synthase kinase 3 (GSK3) is highly involved in skeletal muscle physiology and metabolism, acting as a negative regulator of muscle size, strength, adaptive thermogenesis, and glucose homeostasis. Correspondingly, we have shown that partial knockdown (~40%) of GSK3 specifically in skeletal muscle increases lean mass, reduces fat mass, and activates muscle-based adaptive thermogenesis via sarco(endo)plasmic reticulum Ca2+ (SERCA) uncoupling in male mice. However, the effects of GSK3 knockdown in female mice have yet to be investigated. Here, we examined the effects of muscle-specific GSK3 knockdown on body composition, muscle size and strength, and whole body metabolism in female C57BL/6J mice. Our results show that GSK3 content is higher in the female soleus versus the male soleus; however, there were no differences in the extensor digitorum longus (EDL). Furthermore, muscle-specific GSK3 knockdown did not alter body composition in female mice, nor did it alter daily energy expenditure, glucose/insulin tolerance, mitochondrial respiration, or the expression of the SERCA uncouplers sarcolipin and neuronatin. We also did not find any differences in soleus muscle size, strength, or fatigue resistance. In the EDL, we found that an increase in absolute and specific force production, but there were no differences in fatigability. Therefore, our study highlights sex differences in the response to genetic reduction of gsk3, with most of the effects previously observed in male mice being absent in females. [ABSTRACT FROM AUTHOR]

Purpose: Bilateral force deficit occurs when the maximal generated force during simultaneous bilateral muscle contractions is lower than the sum of forces generated unilaterally. Neural inhibition is stated as the main source for bilateral force deficit. Based on differences in bilateral neural organization, there might be a pronounced neural inhibition for proximal compared to distal effectors. The aim of the present experiment was to evaluate potential differences in bilateral force deficit in proximal compared to distal effectors in lower extremities. Methods: Fifteen young adults performed single-joint maximal voluntary contractions in isometric dorsiflexion of ankle (distal) and knee (proximal) extension unilaterally and bilaterally. Results: Results showed a significant absolute bilateral force deficit for both proximal (123.46 ± 59.51 N) and distal effectors (33.00 ± 35.60 N). Interestingly, the relative bilateral force deficit for knee extension was significantly larger compared to dorsiflexion of ankle, 19.98 ± 10.04% and 10.27 ± 9.57%, respectively. Our results indicate a significantly higher bilateral force deficit for proximal effectors compared to distal effectors. Conclusion: Plausible explanations are related to neuroanatomical and neurophysiological differences between proximal effectors and distal effectors where proximal muscles have a higher potential for bilateral communication compared to distal muscles. In addition, higher forces produced with proximal effectors could cause a higher perceived exertion and cause a more pronounced bilateral force deficit to proximal effectors. [ABSTRACT FROM AUTHOR]

While there is established evidence supporting the involvement of the sense of touch in various actions, the neural underpinnings of touch and action interplay in a social context remain poorly understood. To prospectively investigate this phenomenon and offer further insights, we employed a combination of motor and sensory components by asking participants to imagine exerting force with the index finger while experiencing their own touch, the touch of one another individual, the touch of a surface, and no touch. Based on the assumption that the patterns of activation in the motor system are similar when action is imagined or actually performed, we proceeded to apply a singlepulse transcranial magnetic stimulation over the primary motor cortex (M1) while participants engaged in the act of imagination. Touch experience was associated with higher M1 excitability in the presence and in the absence of force production imagination, but only during force production imagination M1 excitability differed among the types of touch: both biological sources, the selftouch and the touch of one other individual, elicited a significant increase in motor system activity when compared to touching a non-living surface or in the absence of touch. A strong correlation between individual touch avoidance questionnaire values and facilitation in the motor system was present while touching another person, indicating a social aspect for touch in action. The present study unveils the motor system correlates when the sensory/motor components of touch are considered in social contexts. [ABSTRACT FROM AUTHOR]

Background: Alpine skiing involves the conversion of potential energy into kinetic energy, with the "velocity barrier" (VB) at each moment corresponding to the maximal velocity at which the athlete can ski while staying within the boundaries of the gates and maintaining control. Nevertheless, this concept has never been proven by evidence. The aim of this study was to experimentally test the existence of the VB and clarify its relationship with skier's force production/application capacities. Methods: Fourteen skiers were equipped with ski‐mounted force plates and a positional device and ran a 2‐turn Giant Slalom section starting from eight different heights on the slope. Three conditions were selected for further analysis: minimal entrance velocity (vmin); entrance velocity allowing the better section time (VB); maximal entrance velocity (vmax). Entrance velocity, section time, mean force output, ratio of force application effectiveness, velocity normalized energy dissipation, and path length were compared between the three conditions. Moreover, skier's mechanical energy and velocity curves were compared all along the section between the three conditions using SPM analysis. Results: The section time was reduced in VB compared to vmin (p < 0.001) and vmax (p = 0.002). Skiers presented an incapacity to increase force output beyond the VB (p = 0.441) associated with a lower force application effectiveness (p = 0.005). Maximal entrance velocity was associated to higher energy dissipation (p < 0.001) and path length (p = 0.005). Conclusion: The present study experimentally supports the existence of the VB. The force production/application capacities seem to limit the skiing effectiveness beyond the VB, associated to increased energy dissipations and path length. [ABSTRACT FROM AUTHOR]

Abstract Background The effectiveness of strength training with free-weight vs. machine equipment is heavily debated. Thus, the purpose of this meta-analysis was to summarize the data on the effect of free-weight versus machine-based strength training on maximal strength, jump height and hypertrophy. Methods The review was conducted in accordance with the preferred reporting items for systematic reviews and meta-analyses (PRISMA) guidelines, and the systematic search of literature was conducted up to January 1st, 2023. Studies that directly compared free-weight vs. machine-based strength training for a minimum of 6 weeks in adults (18–60 yrs.) were included. Results Thirteen studies (outcomes: maximal strength [n = 12], jump performance [n = 5], muscle hypertrophy [n = 5]) with a total sample of 1016 participants (789 men, 219 women) were included. Strength in free-weight tests increased significantly more with free-weight training than with machines (SMD: -0.210, CI: -0.391, -0.029, p = 0.023), while strength in machine-based tests tended to increase more with machine training than with free-weights (SMD: 0.291, CI: -0.017, 0.600, p = 0.064). However, no differences were found between modalities in direct comparison (free-weight strength vs. machine strength) for dynamic strength (SMD: 0.084, CI: -0.106, 0.273, p = 0.387), isometric strength (SMD: -0.079, CI: -0.432, 0.273, p = 0.660), countermovement jump (SMD: -0.209, CI: -0.597, 0.179, p = 0.290) and hypertrophy (SMD: -0.055, CI: -0.397, 0.287, p = 0.751). Conclusion No differences were detected in the direct comparison of strength, jump performance and muscle hypertrophy. Current body of evidence indicates that strength changes are specific to the training modality, and the choice between free-weights and machines are down to individual preferences and goals.

While there is established evidence supporting the involvement of the sense of touch in various actions, the neural underpinnings of touch and action interplay in a social context remain poorly understood. To prospectively investigate this phenomenon and offer further insights, we employed a combination of motor and sensory components by asking participants to imagine exerting force with the index finger while experiencing their own touch, the touch of one another individual, the touch of a surface, and no touch. Based on the assumption that the patterns of activation in the motor system are similar when action is imagined or actually performed, we proceeded to apply a single-pulse transcranial magnetic stimulation over the primary motor cortex (M1) while participants engaged in the act of imagination. Touch experience was associated with higher M1 excitability in the presence and in the absence of force production imagination, but only during force production imagination M1 excitability differed among the types of touch: both biological sources, the self-touch and the touch of one other individual, elicited a significant increase in motor system activity when compared to touching a non-living surface or in the absence of touch. A strong correlation between individual touch avoidance questionnaire values and facilitation in the motor system was present while touching another person, indicating a social aspect for touch in action. The present study unveils the motor system correlates when the sensory/motor components of touch are considered in social contexts.

We applied the recently introduced concept of intramuscle synergies in spaces of motor units (MUs) to quantify indexes of such synergies in the tibialis anterior during ankle dorsiflexion force production tasks and their changes with fatigue. We hypothesized that MUs would be organized into robust groups (MU modes), which would covary across trials to stabilize force magnitude, and the indexes of such synergies would drop under fatigue. Healthy, young subjects (n = 15; 8 females) produced cyclical, isometric dorsiflexion forces while surface electromyography was used to identify action potentials of individual MUs. Principal component analysis was used to define MU modes. The framework of the uncontrolled manifold (UCM) was used to analyze intercycle variance and compute the synergy index, DVZ. Cyclical force production tasks were repeated after a nonfatiguing exercise (control) and a fatiguing exercise. Across subjects, fatigue led, on average, to a 43% drop in maximal force and fewer identified MUs per subject (29.6 ± 2.1 vs. 32.4 ± 2.1). The first two MU modes accounted for 81.2 ± 0.08% of variance across conditions. Force-stabilizing synergies were present across all conditions and were diminished after fatiguing exercise (1.49 ± 0.40) but not control exercise (1.76 ± 0.75). Decreased stability after fatigue was caused by an increase in the amount of variance orthogonal to the UCM. These findings contrast with earlier studies of multieffector synergies demonstrating increased synergy index under fatigue. We interpret the results as reflections of a drop in the gain of spinal reflex loops under fatigue. The findings corroborate an earlier hypothesis on the spinal nature of intramuscle synergies. NEW & NOTEWORTHY Across multielement force production tasks, fatigue of an element leads to increased indexes of force stability (synergy indexes). Here, however, we show that groups of motor units in the tibialis anterior show decreased indexes of force-stabilizing synergies after fatiguing exercise. These findings align intramuscle synergies with spinal mechanisms, in contrast to the supraspinal control of multimuscle synergies. [ABSTRACT FROM AUTHOR]

Background: The effectiveness of strength training with free-weight vs. machine equipment is heavily debated. Thus, the purpose of this meta-analysis was to summarize the data on the effect of free-weight versus machine-based strength training on maximal strength, jump height and hypertrophy. Methods: The review was conducted in accordance with the preferred reporting items for systematic reviews and meta-analyses (PRISMA) guidelines, and the systematic search of literature was conducted up to January 1st, 2023. Studies that directly compared free-weight vs. machine-based strength training for a minimum of 6 weeks in adults (18–60 yrs.) were included. Results: Thirteen studies (outcomes: maximal strength [n = 12], jump performance [n = 5], muscle hypertrophy [n = 5]) with a total sample of 1016 participants (789 men, 219 women) were included. Strength in free-weight tests increased significantly more with free-weight training than with machines (SMD: -0.210, CI: -0.391, -0.029, p = 0.023), while strength in machine-based tests tended to increase more with machine training than with free-weights (SMD: 0.291, CI: -0.017, 0.600, p = 0.064). However, no differences were found between modalities in direct comparison (free-weight strength vs. machine strength) for dynamic strength (SMD: 0.084, CI: -0.106, 0.273, p = 0.387), isometric strength (SMD: -0.079, CI: -0.432, 0.273, p = 0.660), countermovement jump (SMD: -0.209, CI: -0.597, 0.179, p = 0.290) and hypertrophy (SMD: -0.055, CI: -0.397, 0.287, p = 0.751). Conclusion: No differences were detected in the direct comparison of strength, jump performance and muscle hypertrophy. Current body of evidence indicates that strength changes are specific to the training modality, and the choice between free-weights and machines are down to individual preferences and goals. Key point: If your goal is to maximize strength adaptations, the choice between free-weight and machines should be based on individual preference. The principle of specificity applies, which states that you should choose the exercise you want to be stronger in. This is important for athletes who are tested in specific exercises. For muscle hypertrophy one should consider individual preference when choosing between modalities. We speculate that the combination could result in more complete hypertrophy. [ABSTRACT FROM AUTHOR]

The aim of the present study was to compare the effect of chronic concentric or eccentric training on position sense and joint reaction angle, in healthy, untrained young men. Twenty-four men were randomly assigned into a pure concentric (CT) or a pure eccentric (ET) group and performed for 8 weeks, one training session/week, 75 maximal knee extensors contractions. Before and 48 h after the first (W1) and the last (W8) training sessions, knee joint position sense and joint reaction angle were assessed at three different knee angles (i.e. 30°, 45° and 60°). At the same time points, indirect indices of exercise-induced muscle damage (EIMD) were evaluated (i.e. range of motion [ROM], optimal angle, maximum isometric, concentric and eccentric torques, delayed onset muscle soreness [DOMS] and blood creatine kinase concentrations [CK]). Forty-eight hours post W1, position sense, reaction angle and all EIMD indices were significantly changed for both groups (p < 0.05; η2: 0.125–0.618), however, greater alterations were observed after ET. Significant correlations were found, in both groups, between the training-induced changes of position sense, reaction angles and the changes of EIMD biomarkers (r: −0.855–0.825; p < 0.005). No significant changes were found 48 h post W8 for position sense, reaction angle and EIMD indices (p > 0.285) for both CT or ET groups. In conclusion, exercise-induced changes in position sense and reaction angle, were related to the magnitude of EIMD, and not by the type of muscle contraction per se. Highlights Exercise induced changes in position sense and reaction angle, were related to the magnitude of EIMD, and not by the type of muscle contraction per se. After the 1st training session eccentric exercise caused greater disturbances, compared to concentric exercise, in EIMD indices which caused concomitant disturbances to position sense and knee reaction angle. 8 weeks of either eccentric or concentric training leads to preservation of position sense and knee reaction angle 48 h after maximal intensity exercise of either types of muscle contraction. [ABSTRACT FROM AUTHOR]

Age-related effects on motor asymmetry provide insight into changes in cortical activation during aging. To investigate potential changes in manual performance associated with aging, we conducted the Jamar hand function test and the Purdue Pegboard test on young and older adults. All tests indicated reduced motor asymmetry in the older group. Further analysis suggested that a significant decline in dominant (right) hand function resulted in less asymmetric performance in older adults. The finding is inconsistent with the application of the HAROLD model in the motor domain, which assumes improved performance in the non-dominant hand, leading to a reduction of motor asymmetry in older adults. Based on the manual performance in young and older adults, it is suggested that aging reduces manual asymmetry in both force production and manual dexterity due to the reduced performance of the dominant hand. [ABSTRACT FROM AUTHOR]

Congenital myopathies define a genetically heterogeneous group of disorders associated with severe muscle weakness, for which no therapies are currently available. Here we investigated the repurposing of tamoxifen in mouse models of mild or severe forms of centronuclear myopathies due to mutations in BIN1 (encoding amphiphysin 2) or DNM2 (encoding dynamin 2), respectively. Exposure to a tamoxifen-enriched diet from 3 weeks of age resulted in significant improvement in muscle contractility without increase in fibre size in both models, underlying an increase in the capacity of the muscle fibres to produce more force. In addition, the histological alterations were fully rescued in the BIN1 -centronuclear myopathies mouse model. To assess the mechanism of the rescue, transcriptome analyses and targeted protein studies were performed. Although tamoxifen is known to modulate the transcriptional activity of the oestrogen receptors, correction of the disease transcriptomic signature was marginal on tamoxifen treatment. Conversely, tamoxifen lowered the abnormal increase in dynamin 2 protein level in both centronuclear myopathies models. Of note, it was previously reported that dynamin 2 increase is a main pathological cause of centronuclear myopathies. The Akt/mTOR muscle hypertrophic pathway and protein markers of the ubiquitin–proteasome system (the E3 ubiquitin ligase cullin 3) and autophagy (p62) were increased in both models of centronuclear myopathies. Normalization of dynamin 2 level mainly correlated with the normalization of cullin 3 protein level on tamoxifen treatment, supporting the idea that the ubiquitin–proteasome system is a main target for the tamoxifen effect in the amelioration of these diseases. Overall, our data suggest that tamoxifen antagonizes disease development probably through dynamin 2 level regulation. In conclusion, the beneficial effect of tamoxifen on muscle function supports the suggestion that tamoxifen may serve as a common therapy for several autosomal forms of centronuclear myopathies. [ABSTRACT FROM AUTHOR]

Introduction: The architectural features of muscles (muscle thickness (MT), cross-sectional area (CSA), pennation angles) respond to different stimuli (strength training, immobilization, weight-bearing, etc.). It is imperative to understand the changes in architectural features of the muscles to understand their functional changes. While changes in MT and CSA in foot and ankle muscles have been studied, changes in pennation angles have not yet been studied. Objective: This study is aimed to investigate the changes in pennation angles in foot and ankle muscles from non-weightbearing (NWB) to weight-bearing (WB) positions. Study design: This is a retrospective case-control study. Sixty (23.06 ± 3.16 yrs., 74.67 ± 19.72 kgs, 169.50 ± 8.69 cm,) individuals participated in the study. Methods: Pennation angles were measured in vivo using ultrasound imaging in NWB (sitting) and WB (standing) positions. Three images were taken, and the average was calculated, for the following muscles: Tibialis Anterior (TA), Tibialis Posterior (TP), Peroneals, and Abductor Hallucis (AH). The probe was placed at 20%, 30%, and 50% of the shank length for TA, TP, and peroneal muscles, respectively. For AH, the probe was placed 2 cm distal to the medial malleolus. The probe was oriented along the muscle fibers. An independent sample t-test was used to analyze the differences between pennation angles measures in NWB and WB positions. Results: There was no statistically significant difference found between NWB and WB positions for TA, TP and AH muscles. However, pennation angles increased significantly (p=0.04) for peroneal muscles in the WB position compared to NWB. Conclusions: Changes in muscle architecture are associated with changes in muscle function. Changes in pennation angles in peroneal muscles show greater force production in the functional position compared to NWB positions. Pennation angles may be considered when assessing muscle force for peroneal muscles. [ABSTRACT FROM AUTHOR]

We explored the phenomenon of unintentional force drift seen in the absence of visual feedback during knee extension contractions in isometric conditions. Based on the importance of knee extensors for the anti-gravity function, we hypothesized that such force drifts would be slower and smaller compared to those reported for the upper extremities. We also explored possible effects of foot dominance and gender on the force drifts. Young healthy persons produced isometric knee extension contractions to different levels, ranging from 15 to 25% of maximal voluntary contraction force, with the help of visual feedback, and then, the visual feedback was turned off. Force change over the time interval without visual feedback was quantified. In the absence of visual feedback, force drifted to smaller magnitudes. The drift magnitude expressed in percent of the initial force magnitude was smaller for smaller initial force levels, ranging between 8 and 15% of the initial force for the initial force magnitude of 15% and 25% of maximal voluntary contraction force. The time exponent of the force drift was independent of the initial force magnitude and was, on average, 6.45 s. There were no significant effects of foot dominance or gender, although the male subjects tended to show stronger scaling of the drift magnitude with the initial force level compared to the female subjects. The results show that unintentional force drift is a common phenomenon across limbs and muscle groups. This conclusion fits the theory of control with spatial referent coordinates and the general tendency of all natural systems to drift to states with lower potential energy. [ABSTRACT FROM AUTHOR]

The concept of synergies has been used to address the grouping of motor elements contributing to a task with the covariation of these elements reflecting task stability. This concept has recently been extended to groups of motor units with parallel scaling of the firing frequencies with possible contributions of intermittent recruitment (MU-modes) in compartmentalized flexor and extensor muscles of the forearm stabilizing force magnitude in finger pressing tasks. Here, we directly test for the presence and behavior of MU-modes in the tibialis anterior, a non-compartmentalized muscle. Ten participants performed an isometric cyclical dorsiflexion force production task at 1 Hz between 20 and 40% of maximal voluntary contraction and electromyographic (EMG) data were collected from two high-density wireless sensors placed on the skin over the right tibialis anterior. EMG data were decomposed into individual motor unit frequencies and resolved into sets of MU-modes. Inter-cycle analysis of MU-mode magnitudes within the framework of the uncontrolled manifold (UCM) hypothesis was used to quantify force-stabilizing synergies. Two or three MU-modes were identified in all participants and trials accounting, on average, for 69% of variance and were robust to cross-validation measurements. Strong dorsiflexion force-stabilizing synergies in the space of MU-modes were present in all participants and for both electrode locations as reflected in variance within the UCM (median 954, IQR 511–1924) exceeding variance orthogonal to the UCM (median 5.82, IQR 2.9–17.4) by two orders of magnitude. In contrast, MU-mode-stabilizing synergies in the space of motor unit frequencies were not present. This study offers strong evidence for the existence of synergic control mechanisms at the level of motor units independent of muscle compartmentalization, likely organized within spinal cord circuitry. [ABSTRACT FROM AUTHOR]

Kristiansen, M, Sydow Krogh Pedersen, A-M, Sandvej, G, Jørgensen, P, Jakobsen, JV, de Zee, M, Hansen, EA, and Klitgaard, KK. Enhanced maximal upper-body strength increases performance in sprint kayaking. J Strength Cond Res 37(4): e305–e312, 2023—The association between upper-body strength and performance in 200-m flat-water sprint kayak is not fully elucidated. Therefore, the aim of study 1 was to investigate the relationship between upper-body strength and kayaking performance. In study 2, the aim was to perform a randomized training intervention to investigate whether a causal relationship was present between an increase in strength and an actual change in 200-m kayaking performance. In study 1, 37 (22 men and 15 women) elite kayak paddlers performed tests of maximal power output, isometric force, 1 repetition maximum (1RM), and 40 seconds of maximal repetition number in bench press and bench pull and a 30-second all-out on-water sprint kayak test. In study 2, 26 (16 men and 10 women) national elite junior A, U23, and senior kayak paddlers were allocated into 2 groups: a training group (TRAIN) and a maintenance group (MAIN). Each group completed a 6-week strength training intervention with the purpose of either increasing 1RM in bench press (TRAIN) or maintaining strength (MAIN). Pre- and posttests were performed in 200-m kayak ergometer sprint, 1RM bench press, and 1RM bench pull. In study 1, 1RM in bench press was the best predictor of 30-second on-water kayaking performance with a regression coefficient of 0.474. In study 2, TRAIN significantly increased 1RM strength in bench press (pre: 87.3 ± 21.2 kg, post: 93.9 ± 21.3 kg, p = 0.001) and bench pull (pre: 84.2 ± 15.3 kg, post: 86.0 ± 15.1 kg, p = 0.025). In the 200-m kayak ergometer sprint test, TRAIN significantly decreased the time to complete the test (pre: 44.8 ± 4.3 seconds, post: 44.3 ± 4.3 seconds, p = 0.042). In bench press, 1RM was the best predictor of 200-m kayaking, and an increase in bench press 1RM resulted in increased kayaking performance. [ABSTRACT FROM AUTHOR]

Skeletal muscle fibers are giant multinucleated cells wherein individual nuclei govern the protein synthesis in a finite volume of cytoplasm; this is termed the myonuclear domain (MND). The factors that control MND size remain to be defined. In the present study, we studied the contribution of the NAD+ -dependent deacetylase, sirtuin 1 (SIRT1), to the regulation of nuclear number and MND size. For this, we isolated myofibers from mice with tissue-specific inactivation (mKO) or inducible overexpression (imOX) of SIRT1 and analyzed the 3D organisation of myonuclei. In imOX mice, the number of nuclei was increased whilst the average MND size was decreased as compared to littermate controls. Our findings were the opposite in mKO mice. Muscle stem cell (satellite cell) numbers were reduced in mKO muscles, a possible explanation for the lower density of myonuclei in these mice; however, no change was observed in imOX mice, suggesting that other factors might also be involved, such as the functional regulation of stem cells/muscle precursors. Interestingly, however, the changes in the MND volume did not impact the force-generating capacity of muscle fibers. Taken together, our results demonstrate that SIRT1 is a key regulator of MND sizes, although the underlying molecular mechanisms and the cause-effect relationship between MND and muscle function remain to be fully defined.

This study sought to identify kinetic and electromyographic subphase characteristics distinguishing good from poor jumpers during countermovement vertical jumps (CMVJs), as defined by the reactive strength index (RSI, CMVJ displacement divided by jump time; cutoff = 0.46 m·s−1). A total of 15 men (1.8 [0.6] m, 84.5 [8.5] kg, 24 [2] y) were stratified by RSI into good (n = 6; RSI = 0.57 [0.07] m·s−1) and poor (n = 9; RSI = 0.39 [0.06] m·s−1) performance groups. The following variables were compared between groups using independent t tests (α =.05) and Cohen’s d effect sizes (d ≥ 0.8, large): jump height, propulsive impulse, eccentric rate of force development, and jump time, unloading, eccentric, and concentric subphase times, and average electromyographic amplitudes of 8 lower extremity muscles. Compared with the poor RSI group, the good RSI group exhibited a greater, though not statistically different CMVJ displacement (d = 1.07, P =.06). In addition, the good RSI group exhibited a significantly greater propulsive impulse (P =.04, d = 1.27) and a significantly more rapid unloading subphase (P =.04, d = 1.08). No other significant or noteworthy differences were detected. Enhanced RSI appears related to a quicker unloading phase, allowing a greater portion of the total jumping phase to be utilized generating positive net force. Poor jumpers should aim to use unloading strategies that emphasize quickness to enhance RSI during CMVJ. [ABSTRACT FROM AUTHOR]

In many sports, practitioners must reach their maximal jump height (hmax) under time constraints. This requires a reduction of the countermovement depth and so of the push-off distance (hPO). The purpose of this study was to investigate how hPO influences force-velocity (F-v) profiles (F¯0, v¯0, P¯max, and SFv) and performance. Eleven participants (age: 26 [5] y, height: 175.6 [11.2] cm, mass: 76 [15] kg; squat 1RM: 129 [34] kg) performed maximal countermovement jumps. Kinetic and kinematic measurements were used to assess individual F-v profiles for 3 different hPO conditions (hPO-SMALL, hPO-MEDIUM, hPO-LARGE) from countermovement jumps performed under different load conditions (bodyweight [BW], BW + 8 kg, BW + 17 kg, BW + 40%1RM, BW + 70%1RM). Results indicated that F¯0 and P¯max changed across hPO conditions, while v¯0 remained constant. A lower hPO led to a significantly higher F¯0 and P¯max. These changes resulted in a steeper SFv leading to a more force-oriented profile, a lower optimal SFv and a greater F-v imbalance. Reducing hPO and modifying F-v profile led, to some extent, to a reduction in hmax. Performance is a compromise between hPO, P¯max, and F-v imbalance, all influenced by countermovement depth. This explains why reducing countermovement depth to meet time constraint may lower performance.

Following injury, skeletal muscle undergoes repair via satellite cell (SC)-mediated myogenic progression. In SCs, the circadian molecular clock gene, Bmal1, is necessary for appropriate myogenic progression and repair with evidence that muscle molecular clocks can also affect force production. Utilizing a mouse model allowing for inducible depletion of Bmal1 within SCs, we determined contractile function, SC myogenic progression and muscle damage and repair following eccentric contractile-induced injury. At baseline, SC-Bmal1 iKO animals exhibited a ~20-25% reduction in normalized force production (ex vivo and in vivo) versus control SC-Bmal1 Cntrl and SC-Bmal1 iKO untreated littermates (p < .05). Following contractile injury, SC-Bmal1 iKO animals displayed reduced muscle damage and subsequent repair post-injury (Dystrophin negative fibers 24 h: SC-Bmal1 Cntrl 199 ± 41; SC-Bmal1 iKO 36 ± 13, p < .05) (eMHC + fibers 7 day: SC-Bmal1 Cntrl 217.8 ± 115.5; SC-Bmal1 iKO 27.8 ± 17.3; Centralized nuclei 7 day: SC-Bmal1 Cntrl 160.7 ± 70.5; SC-Bmal1 iKO 46.2 ± 15.7). SC-Bmal1 iKO animals also showed reduced neutrophil infiltration, consistent with less injury (Neutrophil content 24 h: SC-Bmal1 Cntrl 2.4 ± 0.4; SC-Bmal1 iKO 0.4 ± 0.2, % area fraction, p < .05). SC-Bmal1 iKO animals had greater SC activation/proliferation at an earlier timepoint (p < .05) and an unexplained increase in activation 7 days post injury. Collectively, these data suggest SC-Bmal1 plays a regulatory role in force production, influencing the magnitude of muscle damage/repair, with an altered SC myogenic progression following contractile-induced muscle injury., (© 2025 The Author(s). The FASEB Journal published by Wiley Periodicals LLC on behalf of Federation of American Societies for Experimental Biology.)

The purpose of this study was to investigate the influence of push-off distance on maximal jump height and to characterize the relationships between vertical jump mechanical parameters. Seventeen CrossFit athletes performed maximal countermovement jumps on two force-plates, with four different push-off distances induced by different countermovement depths. Results confirmed that push-off distance and maximal jump height were related by a quadratic relationship, and that exists an individual push-off distance maximizing maximal jump height. Furthermore, the increase in push-off distance was associated with an increase in the time of push-off and a concomitant decrease in the mean vertical external force, changing the relative net vertical impulse and so maximal jump height. An almost perfect negative linear relationship was observed between mean vertical external force and time of push-off (r = −0.99, p = 0.006), mathematically resulting in a quadratic relationship between relative net vertical impulse and time of push-off (r = 1.00, p < 0.001). This negative linear relationship could be explained by changes in joint angles and velocities associated with an increase in push-off distance, modifying joint torque production capacities during vertical jumping. This provides new insights to characterize vertical jump mechanical parameters and ways to optimize training and jump performance. [ABSTRACT FROM AUTHOR]

Life is driven by awe-inspiring coordinated movements observed in cells and tissues. In each cell, nm-size molecular motor proteins contribute to these movements as they power numerous mechanical processes with precision and complex orchestration. For the multiple functions that an eukaryotic cell accomplish, motility is essential both at molecular and cellular scales. Tissue morphogenesis, cell migration, cell division or cell differentiation are all controlled by the precise action of such nanomotors that work on cytoskeletal tracks using ATP as fuel. The study of motility has a long history and scientists of all disciplines have contributed to its understanding. The first part of this review compares myosin and kinesin motors to describe the principles underlying how motors convert chemical energy into mechanical movement. In a second part, I will describe how sequence differences selected through evolution can lead to distinct force production output despite a common mechanism. Motors within a superfamily can thus carry out distinct functions in cells. Such differences give rise to their individual, specific motility properties, including reversal of directionality or ability to organize cytoskeletal tracks. The power of structural biology to reveal unexpected and surprising structures, with certainty when visualized at atomic resolution, has been a great advantage for this field. The critical insights gained from the structures can be carefully tested with functional experiments, leading to progress in defining the role motors play in cells. Last, I will describe how targeting these motors can be beneficial for human health. Allosteric sites for specific small molecules can act as activators or inhibitors of the force produced by these nanomotors. While frequent sites of mutations in these motors can lead to disease phenotypes, high therapeutic potential of allosteric effectors is now established for heart muscle diseases and should be extended to treat other pathologies.

Knee extension and hip flexion range of motion (ROM) and functional performance of the hamstrings are of great importance in many sports. The aim of this study was to investigate if static stretching (SS) or vibration foam rolling (VFR) induce greater changes in ROM, functional performance, and stiffness of the hamstring muscles. Twenty-five male volunteers were tested on two appointments and were randomly assigned either to a 2 min bout of SS or VFR. ROM, counter movement jump (CMJ) height, maximum voluntary isometric contraction (MVIC) peak torque, passive resistive torque (PRT), and shear modulus of semitendinosus (ST), semimembranosus (SM), and biceps femoris (BFlh), were assessed before and after the intervention. In both groups ROM increased (SS = 7.7%, P < 0.01; VFR = 8.8%, P < 0.01). The MVIC values decreased after SS (-5.1%, P < 0.01) only. Shear modulus of the ST changed for -6.7% in both groups (VFR: P < 0.01; SS: P < 0.01). Shear modulus decreased in SM after VFR (-6.5%; P = 0.03) and no changes were observed in the BFlh in any group (VFR = -1%; SS = -2.9%). PRT and CMJ values did not change following any interventions. Our findings suggest that VFR might be a favorable warm-up routine if the goal is to acutely increase ROM without compromising functional performance. [ABSTRACT FROM AUTHOR]

Clinical Question: Does ankle dorsiflexion promote irradiation of ipsilateral lower extremity musculature during a maximal isometric quadriceps contraction task in a healthy population? Clinical Bottom Line: There is preliminary evidence supporting the use of active ankle dorsiflexion during isometric quadriceps exercises to promote increased quadriceps activation and force production. As isometric quadriceps exercises are often included in initial stage rehabilitation, increasing muscle activation and force production may be beneficial. [ABSTRACT FROM AUTHOR]

Abstract X-linked myotubular myopathy (XLMTM) is a life-threatening skeletal muscle disease caused by mutations in the MTM1 gene. XLMTM fibres display a population of nuclei mispositioned in the centre. In the present study, we aimed to explore whether positioning and overall distribution of nuclei affects cellular organization and contractile function, thereby contributing to muscle weakness in this disease. We also assessed whether gene therapy alters nuclear arrangement and function. We used tissue from human patients and animal models, including XLMTM dogs that had received increasing doses of recombinant AAV8 vector restoring MTM1 expression (rAAV8-cMTM1). We then used single isolated muscle fibres to analyze nuclear organization and contractile function. In addition to the expected mislocalization of nuclei in the centre of muscle fibres, a novel form of nuclear mispositioning was observed: irregular spacing between those located at the fibre periphery, and an overall increased number of nuclei, leading to dramatically smaller and inconsistent myonuclear domains. Nuclear mislocalization was associated with decreases in global nuclear synthetic activity, contractile protein content and intrinsic myofilament force production. A contractile deficit originating at the myofilaments, rather than mechanical interference by centrally positioned nuclei, was supported by experiments in regenerated mouse muscle. Systemic administration of rAAV8-cMTM1 at doses higher than 2.5 × 1013 vg kg−1 allowed a full rescue of all these cellular defects in XLMTM dogs. Altogether, these findings identify previously unrecognized pathological mechanisms in human and animal XLMTM, associated with myonuclear defects and contractile filament function. These defects can be reversed by gene therapy restoring MTM1 expression in dogs with XLMTM.

Neuromuscular electrical stimulation (NMES) is often used to activate muscles impaired after spinal cord injury to elicit functional activities or to facilitate exercise. However, in addition to the cost and availability of NMES and the inherent muscle fatigue that is associated with its use may limit its widespread utilization. Optimizing stimulation parameters during NMES-induced contractions could maximize force production with less fatigue. Purpose: To examine the interrelationship of pulse duration and pulse frequency on torque production and muscle fatigue in both impaired and non-impaired skeletal muscle of men and women. Methods: Individuals with [ n  = 14 (6 females), 38 ± 13 yr; 175 ± 11 cm; 76 ± 20 kg] and without [ n  = 14 (6 females), 29 ± 8 yr; 175 ± 9 cm; 74 ± 14 kg] spinal cord injury (SCI) participated. Muscle torque was recorded during a series of NMES-induced isometric muscle contractions using different combinations of pulse durations and frequencies. Additionally, two different muscle fatigue protocols (20 and 50 Hz/200µs) were utilized to elicit repeat isometric muscle contractions (1s on and 1s off × 3 min). Results: There was a statistically significant linear trend for pulse charge (the product of pulse frequency and pulse duration) on isometric torque production in participants without (p < 0.001, η 2  = 0.79), and in participants with SCI (p < 0.001, η 2  = 0.66), with higher total pulse charge generating higher torque values. Participants with SCI had significantly greater muscle fatigue for both muscle fatigue protocols (p < 0.05). Conclusions: NMES protocols should consider using longer pulse durations with lower frequencies to maximize force production for individuals with SCI. However, because mechanisms of muscle fatigue may be different for impaired muscle when compared to non-impaired muscle, further studies on protocols to offset fatigue are warranted.

• Corticomotor responses to a single bout of strength exercise vary between younger and older adults. • Following a single bout of strength exercise, older adults exhibit a prolonged reduction in neural drive. • There was no effect of a single bout of strength exercise on intracortical facilitation or inhibition. Evidence shows corticomotor plasticity diminishes with age. Nevertheless, whether strength-training, a proven intervention that induces corticomotor plasticity in younger adults, also takes effect in older adults, remains untested. This study examined the effect of a single-session of strength-exercise on corticomotor plasticity in older and younger adults. Thirteen older adults (72.3 ± 6.5 years) and eleven younger adults (29.9 ± 6.9 years), novice to strength-exercise, participated. Strength-exercise involved four sets of 6–8 repetitions of a dumbbell biceps curl at 70–75% of their one-repetition maximum (1-RM). Muscle strength, cortical, corticomotor and spinal excitability, before and up to 60-minutes after the strength-exercise session were assessed. We observed significant changes over time (p < 0.05) and an interaction between time and age group (p < 0.05) indicating a decrease in corticomotor excitability (18% p < 0.05) for older adults at 30- and 60-minutes post strength-exercise and an increase (26% and 40%, all p < 0.05) in younger adults at the same time points. Voluntary activation (VA) declined in older adults immediately post and 60-minutes post strength-exercise (36% and 25%, all p < 0.05). Exercise had no effect on the cortical silent period (cSP) in older adults however, in young adults cSP durations were shorter at both 30- and 60- minute time points (17% 30-minute post and 9% 60-minute post, p < 0.05). There were no differences in short-interval cortical inhibition (SICI) or intracortical facilitation (ICF) between groups. Although the corticomotor responses to strength-exercise were different within groups, overall, the neural responses seem to be independent of age. [Display omitted] [ABSTRACT FROM AUTHOR]

Abstract Background Originally, the cranks of a handcycle were mounted with a 180° phase shift (asynchronous). However, as handcycling became more popular, the crank mode switched to a parallel mounting (synchronous) over the years. Differences between both modes have been investigated, however, not into great detail for propulsion technique or practice effects. Our aim is to compare both crank modes from a biomechanical and physiological perspective, hence considering force and power production as a cause of physiological outcome measures. This is done within a practice protocol, as it is expected that motor learning takes place in the early stages of handcycling in novices. Methods Twelve able-bodied male novices volunteered to take part. The experiment consisted of a pre-test, three practice sessions and a post-test, which was subsequently repeated for both crank modes in a counterbalanced manner. In each session the participants handcycled for 3 × 4 minutes on a leveled motorized treadmill at 1.94 m/s. Inbetween sessions were 2 days of rest. 3D forces, handlebar and crank angle were measured on the left hand side. Kinematic markers were placed on the handcycle to monitor the movement on the treadmill. Lastly, breath-by-breath spirometry combined with heart-rate were continuously measured. The effects of crank mode and practice-based learning were analyzed using a two way repeated measures ANOVA, with synchronous vs asynchronous and pre-test vs post-test as within-subject factors. Results In the pre-test, asynchronous handcycling was less efficient than synchronous handcycling in terms of physiological strain, force production and timing. At the post-test, the metabolic costs were comparable for both modes. The force production was, also after practice, more efficient in the synchronous mode. External power production, crank rotation velocity and the distance travelled back and forwards on the treadmill suggest that asynchronous handcycling is more constant throughout the cycle. Conclusions As the metabolic costs were reduced in the asynchronous mode, we would advise to include a practice period, when comparing both modes in scientific experiments. For handcycle users, we would currently advise a synchronous set-up for daily use, as the force production is more effective in the synchronous mode, even after practice.

Background: Heart failure (HF) is the leading cause of death in western countries. Cardiac dysfunction is accompanied by skeletal alterations resulting in muscle weakness and fatigue. Exercise is an accepted interventional approach correcting cardiac and skeletal dysfunction, thereby improving mortality, re-hospitalization and quality of life. Animal models are used to characterize underpinning mechanisms. Transverse aortic constriction (TAC) results in cardiac pressure overload and finally HF. Whether exercise training improves cardiac remodeling and peripheral cachexia in the TAC mouse model was not analyzed yet. In this study, 2 weeks post TAC animals were randomized into two groups either performing a moderate exercise program (five times per week at 60% VO2 max for 40 min for a total of 8 weeks) or staying sedentary. Results: In both TAC groups HF characteristics reduced ejection fraction (− 15% compared to sham, p < 0.001), cardiac remodeling (+ 22.5% cardiomyocyte cross sectional area compared to sham; p < 0.001) and coronary artery congestion (+ 34% diameter compared to sham; p = 0.008) were observed. Unexpectedly, peripheral cachexia was not detected. Furthermore, compared to sedentary group animals from the exercise group showed aggravated HF symptoms [heart area + 9% (p = 0.026), heart circumference + 7% (p = 0.002), right ventricular wall thickness − 30% (p = 0.003)] while muscle parameters were unchanged [Musculus soleus fiber diameter (p = 0.55), Musculus extensor digitorum longus contraction force (p = 0.90)]. Conclusion: The severe TAC model is inappropriate to study moderate exercise effects in HF with respect to cardiac and skeletal muscle improvements. Further, the phenotype induced by different TAC procedures should be well documented and taken into account when planning experiments. [ABSTRACT FROM AUTHOR]

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The aim of this study was to compare biomechanical and perceptual-cognitive variables between sexes during an offensive and defensive agility protocol. Twelve male and female (n = 24) recreational team sport athletes participated in this study, each performing 12 offensive and defensive agility trials (6 left, 6 right) changing direction in response to movements of a human stimulus. Three-dimensional motion, ground reaction force (GRF), and impulse data were recorded across plant phase for dominant leg change of direction (COD) movements, while timing gates and high-speed video captured decision time, total running time, and post COD stride velocity. Subjects also performed a unilateral isometric squat to determine lower body strength and limb dominance. Group (sex) by condition (2 × 2) MANOVAs with follow-up ANOVAs were conducted to examine differences between groups (P ≤ .05). Male athletes demonstrated significantly greater lower body strength, vertical braking force and impulse application, knee and spine flexion, and hip abduction, as well as faster decision time and post COD stride velocity during both agility conditions compared with females. Differences between offensive and defensive movements appear to be attributed to differences in decision time between sexes. This study demonstrates that biomechanical and perceptual-cognitive differences exist between sexes and within offensive and defensive agility movements. [ABSTRACT FROM AUTHOR]

Kinesin motor proteins hydrolyze ATP to produce force for spindle assembly and vesicle transport, performing essential functions in cell division and motility, but the structural changes required for force generation are uncertain. We now report high-resolution structures showing new transitions in the kinesin mechanochemical cycle, including power stroke fluctuations upon ATP binding and a post-hydrolysis state with bound ADP + free phosphate. We find that rate-limiting ADP release occurs upon microtubule binding, accompanied by central β-sheet twisting, which triggers the power stroke - stalk rotation and neck mimic docking - upon ATP binding. Microtubule release occurs with β-strand-to-loop transitions, implying that β-strand refolding induces Pi release and the recovery stroke. The strained β-sheet during the power stroke and strand-to-loop transitions identify the β-sheet as the long-sought motor spring., Competing Interests: Competing interests: The authors declare that they have no competing interests.

The aim of this study was to propose a new index called Postural Force Production Index (PFPI) for evaluating the force production during handcycling. For a given posture, it assesses the force generation capacity in all Cartesian directions by linking the joint configuration to the effective force applied on the handgrips. Its purpose is to give insight into the force pattern of handcycling users, and could be used as ergonomic index. The PFPI is based on the force ellipsoid, which belongs to the class of manipulability indices and represents the overall force production capabilities at the hand in all Cartesian directions from unit joint torques. The kinematics and kinetics of the arm were recorded during a 1-min exercise test on a handcycle at 70 revolutions per minute performed by one paraplegic expert in handcycling. The PFPI values were compared with the Fraction Effective Force (FEF), which is classically associated with the effectiveness of force application. The results showed a correspondence in the propulsion cycle between FEF peaks and the most favorable postures to produce a force tangential to the crank rotation (PFPI). This preliminary study opens a promising way to study patterns of force production in the framework of handcycling movement analysis. [ABSTRACT FROM AUTHOR]