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3. Low-level laser therapy to recovery testicular degeneration in rams: effects on seminal characteristics, scrotal temperature, plasma testosterone concentration, and testes histopathology

Author

Alves, Maíra Bianchi Rodrigues, de Arruda, Rubens Paes, Batissaco, Leonardo, Florez-Rodriguez, Shirley Andrea, de Oliveira, Bruna Marcele Martins, Torres, Mariana Andrade, Ravagnani, Gisele Mouro, Lançoni, Renata, de Almeida, Tamie Guibu, Storillo, Vanessa Martins, Vellone, Vinicius Silva, Franci, Celso Rodrigues, Thomé, Helder Esteves, Canella, Carolina Luz, De Andrade, André Furugen Cesar, and Celeghini, Eneiva Carla Carvalho

Published
2016
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7. A calcium-induced calcium release mechanism supports luteinizing hormone-induced testosterone secretion in mouse Leydig cells

Author

Costa, Roberta Ribeiro, Varanda, Wamberto Antonio, and Franci, Celso Rodrigues

Subjects
Calcium channels -- Health aspects, Testosterone -- Health aspects, Luteinizing hormone -- Health aspects, Leydig cells -- Health aspects, Biological sciences
Abstract

Leydig cells are responsible for the synthesis and secretion of testosterone, processes controlled by luteinizing hormone (LH). Binding of LH to a G protein-coupled receptor in the plasma membrane results in an increase in cAMP and in intracellular [Ca.sup.2+] concentration ([[[Ca.sup.2+]].sub.i]). Here we show, using immunofluorescence, that Leydig cells express ryanodine receptors (RyRs) and inositol 1,4,5-trisphosphate receptors (IP3Rs). Measurements of intracellular calcium changes using the fluorescent calcium-sensitive dye fluo-3 and confocal microscopy show that both types of receptors are involved in a calcium-induced calcium release (CICR) mechanism, which amplifies the initial [Ca.sup.2+] influx through plasma membrane T-type calcium channels (Car3). The RyRs and IP3Rs are functional, as judged from both their activation by caffeine and IP3 and block by ryanodine and 2-aminoethoxydiphenyl borate (2-APB), respectively. RyRs are the principal players involved in the release of [Ca.sup.2+] from the endoplasmic reticulum, as evidenced by the fact that global [Ca.sup.2+] changes evoked by LH are readily blocked by 100 [micro]M ryanodine but not by 2-APB or xestospongin C. Finally, steroid production by Leydig cells is inhibited by ryanodine but not by 2-APB. These results not only broaden our understanding of the role played by calcium in Leydig cells but also show, for the first time, that RyRs have an important role in determining testosterone secretion by the testis. ryanodine receptor; inositol 1,4,5-trisphosphate receptor doi: 10.1152/ajpcell.00521.2009.

Published
2010

18. Bloqueio do receptor AT1 da Angiotensina II reduz o número de folículos antrais em ratas com obesidade induzida por dieta de cafeteria

Author

Sagae,Sara Cristina, Gobo,Cristiane Gisselda, Paz,Edson Duarte Ribeiro, Menegotto,Juliana Beal, Yamashita,Patrícia Kanae, Franci,Celso Rodrigues, and Balbo,Sandra Lucinei

Subjects
Ratos Wistar, Obesidade, RG1-991, Folículo ovarianano/crescimento & desenvolvimento, Gynecology and obstetrics, Infertilidade, Losartan
Abstract

OBJETIVO: Avaliar o desenvolvimento folicular em ratas Wistar com obesidade induzida por dieta de cafeteria (DCAF) submetidas à administração de losartan (LOS), um antagonista do receptor AT1 da Angiotensina II. MÉTODOS: Aos 21 dias de vida, as ratas foram separadas aleatoriamente em dois grupos: controle (CTL), que recebeu ração padrão, e cafeteria (CAF), que recebeu a DCAF, altamente palatável e calórica. Aos 70 dias de vida, início da idade reprodutiva, animais do grupo CAF foram subdivididos em dois grupos (n=15/grupo): CAF, que recebeu água, e CAF+LOS, que recebeu 30 mg/kg de peso corporal (PC) de LOS por gavagem durante 30 dias. O grupo CTL também recebeu água por gavagem. Aos 100 dias de vida foi realizada a eutanásia dos animais e o PC e das gorduras retroperitoneal, perigonadal e subcutânea foi avaliado. Os ovários direitos foram retirados para contagem do número dos diferentes tipos de folículos ovarianos. As concentrações plasmáticas dos hormônios folículo-estimulantes (FSH), luteinizante (LH), prolactina (PRL) e progesterona foram avaliadas. Os resultados foram expressos como média±erro padrão da média. Para análise estatística, foi utilizado one-way ANOVA, seguido pelo pós-teste de Newman-Keuls (p

Published
2015

20. Royal jelly decreases corticosterone levels and improves the brain antioxidant system in restraint and cold stressed rats

Author

Teixeira, Renata Roland, primary, de Souza, Adriele Vieira, additional, Peixoto, Leonardo Gomes, additional, Machado, Helen Lara, additional, Caixeta, Douglas Carvalho, additional, Vilela, Danielle Diniz, additional, Baptista, Nathalia Belele, additional, Franci, Celso Rodrigues, additional, and Espindola, Foued Salmen, additional

Published
2017
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23. Sepsis 2016 Paris

Author

Ingles, Marcia, primary, Crowfoot, Gary, additional, Smelaya, Tamara V., additional, Kuzovlev, Artem N., additional, Salnikova, Lubov E., additional, Bhikoo, Raisa, additional, Khwannimit, Bodin, additional, Bhurayanontachai, Rungsun, additional, Vattanavanit, Veerapong, additional, Tourteau, Emilie, additional, Filali, Amel, additional, van Grunderbeeck, Nicolas, additional, Nigeon, Olivier, additional, Bazus, Hélène, additional, Masse, Juliette, additional, Mallat, Jihad, additional, Thevenin, Didier, additional, Prokhorenko, Isabella, additional, Kabanov, Dmitry, additional, Zubova, Svetlana, additional, Grachev, Sergey, additional, Salcedo, Margarita, additional, Witte, Stephan, additional, Cuvier, Valérie, additional, Derive, Marc, additional, Gibot, Sébastien, additional, Garaud, Jean-Jacques, additional, Kumar, Vijay, additional, Chhibber, Sanjay, additional, Santos, Jerico R., additional, Sevillejal, Jesus Emmanuel A. D., additional, Nevado, Jose B., additional, Linge, Helena M., additional, Ochani, Kanta, additional, Lin, Ke, additional, Lee, Ji Young, additional, Wang, Ping, additional, Tembhre, Manoj, additional, Liu, Shu Fang, additional, Singhal, Pravin C., additional, Miller, Edmund J., additional, HO, Jeffery, additional, Liu, Xiaodong, additional, Kwong, Thomas, additional, Zhang, Lin, additional, Chan, Hung, additional, Wong, Sunny H., additional, Choi, Gordon, additional, Gin, Tony, additional, Chan, Matthew T. V., additional, Wu, William K. K., additional, Vliegen, Gwendolyn, additional, Kehoe, Kaat, additional, Verkerk, Robert, additional, Fransen, Erik, additional, Peters, Esther, additional, Lambeir, Anne-Marie, additional, Pickkers, Peter, additional, Jorens, Philippe G., additional, De Meester, Ingrid, additional, Ribeiro, Aline Barbosa, additional, Souza, Ana Paula Trevelin, additional, Giusti, Humberto, additional, Franci, Celso Rodrigues, additional, Saia, Rafael Simone, additional, Anderko, Renee R., additional, Jackson, Vanessa M., additional, Palmer, Octavia M. Peck, additional, Angus, Derek C., additional, Kellum, John A., additional, Carcillo, Joseph A., additional, Verboom, D. M., additional, Koster-Brouwer, M. E., additional, van de Groep, K., additional, Frencken, J. F., additional, Scicluna, B., additional, Gisbertz, S. S., additional, Henegouwen, M. I. van Berge, additional, Ruurda, J. P., additional, van Hillegersberg, R., additional, van der Poll, T., additional, Bonten, M. J. M., additional, Cremer, O. L., additional, Klouwenberg, P. M. C. Klein, additional, Beloborodova, Natalia, additional, Osipov, Artem, additional, Pautova, Alisa, additional, Bedova, Aleksandra, additional, Mas-Oliva, Jaime, additional, García-González, Victor, additional, Sukhina, Marina, additional, Zhukhovitskiy, Vladimir, additional, Sukhina, Marina A., additional, Obraztsov, Igor, additional, Zhukhovitskiy, Vladimir G., additional, Peronace, Cinzia, additional, Matera, Giovanni, additional, Galati, Luisa, additional, Giancotti, Aida, additional, Barreca, Giorgio Settimo, additional, Quirino, Angela, additional, Liberto, Maria Carla, additional, Focà, Alfredo, additional, Labiad, Yasmine, additional, Venton, Geoffroy, additional, Baier, Céline, additional, Colle, Julien, additional, Farnault, Laure, additional, Brunet, Corinne, additional, Loriod, Béatrice, additional, Fernandez-Nunez, Nicloas, additional, Suchon, Pierre, additional, Mattei, Jean-Camille, additional, Rihet, Pascal, additional, Nguyen, Catherine, additional, Costello, Régis, additional, Tesfai, Abel, additional, Ahmetaj-Shala, Blerina, additional, Gashaw, Hime, additional, Quinlan, Gregory, additional, MacCallum, Niall, additional, Mumby, Sharon, additional, Gray, DNicola, additional, Leiper, James, additional, Kirkby, Nicholas, additional, Mitchell, Jane A., additional, Costa, Luis Henrique A., additional, Catalão, Carlos Henrique R., additional, Santos-Júnior, Nilton N., additional, Souza, Anderson O., additional, Alberici, Luciane C., additional, and Rocha, Maria José A., additional

Published
2016
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24. Bloqueio do receptor AT1 da Angiotensina II reduz o número de folículos antrais em ratas com obesidade induzida por dieta de cafeteria

Author

Sagae, Sara Cristina, Gobo, Cristiane Gisselda, Paz, Edson Duarte Ribeiro, Menegotto, Juliana Beal, Yamashita, Patrícia Kanae, Franci, Celso Rodrigues, and Balbo, Sandra Lucinei

Subjects
Ratos Wistar, Obesidade, Infertility, Folículo ovarianano/crescimento & desenvolvimento, Obesity, Rats, Wistar, Infertilidade, Losartan, Ovarian follicle/growth & development
Abstract

OBJETIVO: Avaliar o desenvolvimento folicular em ratas Wistar com obesidade induzida por dieta de cafeteria (DCAF) submetidas à administração de losartan (LOS), um antagonista do receptor AT1 da Angiotensina II. MÉTODOS: Aos 21 dias de vida, as ratas foram separadas aleatoriamente em dois grupos: controle (CTL), que recebeu ração padrão, e cafeteria (CAF), que recebeu a DCAF, altamente palatável e calórica. Aos 70 dias de vida, início da idade reprodutiva, animais do grupo CAF foram subdivididos em dois grupos (n=15/grupo): CAF, que recebeu água, e CAF+LOS, que recebeu 30 mg/kg de peso corporal (PC) de LOS por gavagem durante 30 dias. O grupo CTL também recebeu água por gavagem. Aos 100 dias de vida foi realizada a eutanásia dos animais e o PC e das gorduras retroperitoneal, perigonadal e subcutânea foi avaliado. Os ovários direitos foram retirados para contagem do número dos diferentes tipos de folículos ovarianos. As concentrações plasmáticas dos hormônios folículo-estimulantes (FSH), luteinizante (LH), prolactina (PRL) e progesterona foram avaliadas. Os resultados foram expressos como média±erro padrão da média. Para análise estatística, foi utilizado one-way ANOVA, seguido pelo pós-teste de Newman-Keuls (p

Published
2015

25. Sepsis 2016 Paris : Paris, France. 6-8 December 2016

Author

Ingles, Marcia, Crowfoot, Gary, Smelaya, Tamara V., Kuzovlev, Artem N., Salnikova, Lubov E., Bhikoo, Raisa, Khwannimit, Bodin, Bhurayanontachai, Rungsun, Vattanavanit, Veerapong, Tourteau, Emilie, Filali, Amel, van Grunderbeeck, Nicolas, Nigeon, Olivier, Bazus, Hélène, Masse, Juliette, Mallat, Jihad, Thevenin, Didier, Prokhorenko, Isabella, Kabanov, Dmitry, Zubova, Svetlana, Grachev, Sergey, Salcedo, Margarita, Witte, Stephan, Cuvier, Valérie, Derive, Marc, Gibot, Sébastien, Garaud, Jean Jacques, Kumar, Vijay, Chhibber, Sanjay, Santos, Jerico R., Sevillejal, Jesus Emmanuel A D, Nevado, Jose B., Linge, Helena M., Ochani, Kanta, Lin, Ke, Lee, Ji Young, Wang, Ping, Tembhre, Manoj, Liu, Shu Fang, Singhal, Pravin C., Miller, Edmund J., HO, Jeffery, Liu, Xiaodong, Kwong, Thomas, Zhang, Lin, Chan, Hung, Wong, Sunny H., Choi, Gordon, Gin, Tony, Chan, Matthew T V, Wu, William K K, Vliegen, Gwendolyn, Kehoe, Kaat, Verkerk, Robert, Fransen, Erik, Peters, Esther, Lambeir, Anne Marie, Pickkers, Peter, Jorens, Philippe G., De Meester, Ingrid, Ribeiro, Aline Barbosa, Souza, Ana Paula Trevelin, Giusti, Humberto, Franci, Celso Rodrigues, Saia, Rafael Simone, Anderko, Renee R., Jackson, Vanessa M., Palmer, Octavia M Peck, Angus, Derek C., Kellum, John A., Carcillo, Joseph A., Verboom, D. M., Koster-Brouwer, M. E., van de Groep, K., Frencken, J. F., Scicluna, B., Gisbertz, S. S., Henegouwen, M. I van Berge, Ruurda, J. P., van Hillegersberg, R., van der Poll, T., Bonten, M. J M, Cremer, O. L., Klouwenberg, P. M C Klein, Beloborodova, Natalia, Osipov, Artem, Pautova, Alisa, Bedova, Aleksandra, Mas-Oliva, Jaime, García-González, Victor, Sukhina, Marina, Zhukhovitskiy, Vladimir, Sukhina, Marina A., Obraztsov, Igor, Zhukhovitskiy, Vladimir G., Peronace, Cinzia, Matera, Giovanni, Galati, Luisa, Giancotti, Aida, Barreca, Giorgio Settimo, Quirino, Angela, Liberto, Maria Carla, Focà, Alfredo, Labiad, Yasmine, Venton, Geoffroy, Baier, Céline, Colle, Julien, Farnault, Laure, Brunet, Corinne, Loriod, Béatrice, Fernandez-Nunez, Nicloas, Suchon, Pierre, Mattei, Jean Camille, Rihet, Pascal, Nguyen, Catherine, Costello, Régis, Tesfai, Abel, Ahmetaj-Shala, Blerina, Gashaw, Hime, Quinlan, Gregory, MacCallum, Niall, Mumby, Sharon, Gray, DNicola N., Leiper, James, Kirkby, Nicholas, Mitchell, Jane A., Costa, Luis Henrique A, Catalão, Carlos Henrique R, Santos-Júnior, Nilton N., Souza, Anderson O., Alberici, Luciane C., Rocha, Maria José A, Ingles, Marcia, Crowfoot, Gary, Smelaya, Tamara V., Kuzovlev, Artem N., Salnikova, Lubov E., Bhikoo, Raisa, Khwannimit, Bodin, Bhurayanontachai, Rungsun, Vattanavanit, Veerapong, Tourteau, Emilie, Filali, Amel, van Grunderbeeck, Nicolas, Nigeon, Olivier, Bazus, Hélène, Masse, Juliette, Mallat, Jihad, Thevenin, Didier, Prokhorenko, Isabella, Kabanov, Dmitry, Zubova, Svetlana, Grachev, Sergey, Salcedo, Margarita, Witte, Stephan, Cuvier, Valérie, Derive, Marc, Gibot, Sébastien, Garaud, Jean Jacques, Kumar, Vijay, Chhibber, Sanjay, Santos, Jerico R., Sevillejal, Jesus Emmanuel A D, Nevado, Jose B., Linge, Helena M., Ochani, Kanta, Lin, Ke, Lee, Ji Young, Wang, Ping, Tembhre, Manoj, Liu, Shu Fang, Singhal, Pravin C., Miller, Edmund J., HO, Jeffery, Liu, Xiaodong, Kwong, Thomas, Zhang, Lin, Chan, Hung, Wong, Sunny H., Choi, Gordon, Gin, Tony, Chan, Matthew T V, Wu, William K K, Vliegen, Gwendolyn, Kehoe, Kaat, Verkerk, Robert, Fransen, Erik, Peters, Esther, Lambeir, Anne Marie, Pickkers, Peter, Jorens, Philippe G., De Meester, Ingrid, Ribeiro, Aline Barbosa, Souza, Ana Paula Trevelin, Giusti, Humberto, Franci, Celso Rodrigues, Saia, Rafael Simone, Anderko, Renee R., Jackson, Vanessa M., Palmer, Octavia M Peck, Angus, Derek C., Kellum, John A., Carcillo, Joseph A., Verboom, D. M., Koster-Brouwer, M. E., van de Groep, K., Frencken, J. F., Scicluna, B., Gisbertz, S. S., Henegouwen, M. I van Berge, Ruurda, J. P., van Hillegersberg, R., van der Poll, T., Bonten, M. J M, Cremer, O. L., Klouwenberg, P. M C Klein, Beloborodova, Natalia, Osipov, Artem, Pautova, Alisa, Bedova, Aleksandra, Mas-Oliva, Jaime, García-González, Victor, Sukhina, Marina, Zhukhovitskiy, Vladimir, Sukhina, Marina A., Obraztsov, Igor, Zhukhovitskiy, Vladimir G., Peronace, Cinzia, Matera, Giovanni, Galati, Luisa, Giancotti, Aida, Barreca, Giorgio Settimo, Quirino, Angela, Liberto, Maria Carla, Focà, Alfredo, Labiad, Yasmine, Venton, Geoffroy, Baier, Céline, Colle, Julien, Farnault, Laure, Brunet, Corinne, Loriod, Béatrice, Fernandez-Nunez, Nicloas, Suchon, Pierre, Mattei, Jean Camille, Rihet, Pascal, Nguyen, Catherine, Costello, Régis, Tesfai, Abel, Ahmetaj-Shala, Blerina, Gashaw, Hime, Quinlan, Gregory, MacCallum, Niall, Mumby, Sharon, Gray, DNicola N., Leiper, James, Kirkby, Nicholas, Mitchell, Jane A., Costa, Luis Henrique A, Catalão, Carlos Henrique R, Santos-Júnior, Nilton N., Souza, Anderson O., Alberici, Luciane C., and Rocha, Maria José A

Published
2016

26. Sepsis 2016 Paris: Paris, France. 6-8 December 2016

Author

Epi Infectieziekten Team 1, Infection & Immunity, Verpleegafd Vaatchirurgie D4 Oost, MS CGO, Cancer, Divisie Beeld & Oncologie, MMB, Epi Infectieziekten, JC onderzoeksprogramma Infectieziekten, Medische Staf Intensive Care, Ingles, Marcia, Crowfoot, Gary, Smelaya, Tamara V., Kuzovlev, Artem N., Salnikova, Lubov E., Bhikoo, Raisa, Khwannimit, Bodin, Bhurayanontachai, Rungsun, Vattanavanit, Veerapong, Tourteau, Emilie, Filali, Amel, van Grunderbeeck, Nicolas, Nigeon, Olivier, Bazus, Hélène, Masse, Juliette, Mallat, Jihad, Thevenin, Didier, Prokhorenko, Isabella, Kabanov, Dmitry, Zubova, Svetlana, Grachev, Sergey, Salcedo, Margarita, Witte, Stephan, Cuvier, Valérie, Derive, Marc, Gibot, Sébastien, Garaud, Jean Jacques, Kumar, Vijay, Chhibber, Sanjay, Santos, Jerico R., Sevillejal, Jesus Emmanuel A D, Nevado, Jose B., Linge, Helena M., Ochani, Kanta, Lin, Ke, Lee, Ji Young, Wang, Ping, Tembhre, Manoj, Liu, Shu Fang, Singhal, Pravin C., Miller, Edmund J., HO, Jeffery, Liu, Xiaodong, Kwong, Thomas, Zhang, Lin, Chan, Hung, Wong, Sunny H., Choi, Gordon, Gin, Tony, Chan, Matthew T V, Wu, William K K, Vliegen, Gwendolyn, Kehoe, Kaat, Verkerk, Robert, Fransen, Erik, Peters, Esther, Lambeir, Anne Marie, Pickkers, Peter, Jorens, Philippe G., De Meester, Ingrid, Ribeiro, Aline Barbosa, Souza, Ana Paula Trevelin, Giusti, Humberto, Franci, Celso Rodrigues, Saia, Rafael Simone, Anderko, Renee R., Jackson, Vanessa M., Palmer, Octavia M Peck, Angus, Derek C., Kellum, John A., Carcillo, Joseph A., Verboom, D. M., Koster-Brouwer, M. E., van de Groep, K., Frencken, J. F., Scicluna, B., Gisbertz, S. S., Henegouwen, M. I van Berge, Ruurda, J. P., van Hillegersberg, R., van der Poll, T., Bonten, M. J M, Cremer, O. L., Klouwenberg, P. M C Klein, Beloborodova, Natalia, Osipov, Artem, Pautova, Alisa, Bedova, Aleksandra, Mas-Oliva, Jaime, García-González, Victor, Sukhina, Marina, Zhukhovitskiy, Vladimir, Sukhina, Marina A., Obraztsov, Igor, Zhukhovitskiy, Vladimir G., Peronace, Cinzia, Matera, Giovanni, Galati, Luisa, Giancotti, Aida, Barreca, Giorgio Settimo, Quirino, Angela, Liberto, Maria Carla, Focà, Alfredo, Labiad, Yasmine, Venton, Geoffroy, Baier, Céline, Colle, Julien, Farnault, Laure, Brunet, Corinne, Loriod, Béatrice, Fernandez-Nunez, Nicloas, Suchon, Pierre, Mattei, Jean Camille, Rihet, Pascal, Nguyen, Catherine, Costello, Régis, Tesfai, Abel, Ahmetaj-Shala, Blerina, Gashaw, Hime, Quinlan, Gregory, MacCallum, Niall, Mumby, Sharon, Gray, DNicola N., Leiper, James, Kirkby, Nicholas, Mitchell, Jane A., Costa, Luis Henrique A, Catalão, Carlos Henrique R, Santos-Júnior, Nilton N., Souza, Anderson O., Alberici, Luciane C., Rocha, Maria José A, Epi Infectieziekten Team 1, Infection & Immunity, Verpleegafd Vaatchirurgie D4 Oost, MS CGO, Cancer, Divisie Beeld & Oncologie, MMB, Epi Infectieziekten, JC onderzoeksprogramma Infectieziekten, Medische Staf Intensive Care, Ingles, Marcia, Crowfoot, Gary, Smelaya, Tamara V., Kuzovlev, Artem N., Salnikova, Lubov E., Bhikoo, Raisa, Khwannimit, Bodin, Bhurayanontachai, Rungsun, Vattanavanit, Veerapong, Tourteau, Emilie, Filali, Amel, van Grunderbeeck, Nicolas, Nigeon, Olivier, Bazus, Hélène, Masse, Juliette, Mallat, Jihad, Thevenin, Didier, Prokhorenko, Isabella, Kabanov, Dmitry, Zubova, Svetlana, Grachev, Sergey, Salcedo, Margarita, Witte, Stephan, Cuvier, Valérie, Derive, Marc, Gibot, Sébastien, Garaud, Jean Jacques, Kumar, Vijay, Chhibber, Sanjay, Santos, Jerico R., Sevillejal, Jesus Emmanuel A D, Nevado, Jose B., Linge, Helena M., Ochani, Kanta, Lin, Ke, Lee, Ji Young, Wang, Ping, Tembhre, Manoj, Liu, Shu Fang, Singhal, Pravin C., Miller, Edmund J., HO, Jeffery, Liu, Xiaodong, Kwong, Thomas, Zhang, Lin, Chan, Hung, Wong, Sunny H., Choi, Gordon, Gin, Tony, Chan, Matthew T V, Wu, William K K, Vliegen, Gwendolyn, Kehoe, Kaat, Verkerk, Robert, Fransen, Erik, Peters, Esther, Lambeir, Anne Marie, Pickkers, Peter, Jorens, Philippe G., De Meester, Ingrid, Ribeiro, Aline Barbosa, Souza, Ana Paula Trevelin, Giusti, Humberto, Franci, Celso Rodrigues, Saia, Rafael Simone, Anderko, Renee R., Jackson, Vanessa M., Palmer, Octavia M Peck, Angus, Derek C., Kellum, John A., Carcillo, Joseph A., Verboom, D. M., Koster-Brouwer, M. E., van de Groep, K., Frencken, J. F., Scicluna, B., Gisbertz, S. S., Henegouwen, M. I van Berge, Ruurda, J. P., van Hillegersberg, R., van der Poll, T., Bonten, M. J M, Cremer, O. L., Klouwenberg, P. M C Klein, Beloborodova, Natalia, Osipov, Artem, Pautova, Alisa, Bedova, Aleksandra, Mas-Oliva, Jaime, García-González, Victor, Sukhina, Marina, Zhukhovitskiy, Vladimir, Sukhina, Marina A., Obraztsov, Igor, Zhukhovitskiy, Vladimir G., Peronace, Cinzia, Matera, Giovanni, Galati, Luisa, Giancotti, Aida, Barreca, Giorgio Settimo, Quirino, Angela, Liberto, Maria Carla, Focà, Alfredo, Labiad, Yasmine, Venton, Geoffroy, Baier, Céline, Colle, Julien, Farnault, Laure, Brunet, Corinne, Loriod, Béatrice, Fernandez-Nunez, Nicloas, Suchon, Pierre, Mattei, Jean Camille, Rihet, Pascal, Nguyen, Catherine, Costello, Régis, Tesfai, Abel, Ahmetaj-Shala, Blerina, Gashaw, Hime, Quinlan, Gregory, MacCallum, Niall, Mumby, Sharon, Gray, DNicola N., Leiper, James, Kirkby, Nicholas, Mitchell, Jane A., Costa, Luis Henrique A, Catalão, Carlos Henrique R, Santos-Júnior, Nilton N., Souza, Anderson O., Alberici, Luciane C., and Rocha, Maria José A

Published
2016

27. Recovery of normal testicular temperature after scrotal heat stress in rams assessed by infrared thermography and its effects on seminal characteristics and testosterone blood serum concentration

Author

Alves, Maíra Bianchi Rodrigues, primary, Andrade, André Furugen Cesar de, additional, Arruda, Rubens Paes de, additional, Batissaco, Leonardo, additional, Florez-Rodriguez, Shirley Andrea, additional, Oliveira, Bruna Marcele Martins de, additional, Torres, Mariana Andrade, additional, Lançoni, Renata, additional, Ravagnani, Gisele Mouro, additional, Prado Filho, Roberto Romano do, additional, Vellone, Vinícius Silva, additional, Losano, João Diego de Agostini, additional, Franci, Celso Rodrigues, additional, Nichi, Marcílio, additional, and Celeghini, Eneiva Carla Carvalho, additional

Published
2016
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28. Progesterone production in superovulated holstein heifers and in crossbred recipient of embryo supplemented with betacarotene and tocopherol

Author

Sales, José Nélio de Sousa, Dias, Lilian Mara Kirsch, Franci, Celso Rodrigues, Rocha, Alexandro Aluísio, Cardoso, Guilherme Gastão, and Souza, José Camisão de

Subjects
reproduction, transferência de embriões, cattle, Vitamina, reprodução, gado, Vitamin, embryo transfer
Abstract

Two experiments were conducted to evaluate the effect of the intramuscular injection of betacarotene associated to tocopherol on the plasma concentration progesterone of superovulated Holstein heifers (experiment 1) and in crossbred (Bos taurus x Bos indicus) heifers submitted to fixed-time embryo transfer (FTET, experiment 2). In experiment 1, after estrus synchronization and superovulation animals were inseminated 12 and 24 hours after estrus onset and embryos flushed 7 days later. Heifers were allocated randomly to one of three treatments: Control; T800 (800 mg of betacarotene plus 500 mg of tocopherol) and T1200 (1,200 mg of betacarotene plus 750 mg of tocopherol). The treatments were given on the day of ear implant placement and repeated on the first day of superovulation. Blood samples were collected on D0, D5, D9, D12 and D16. In experiment 2, treatments were imposed at intravaginal device insertion (D0). The same experimental design, as in experiment 1, was used. Blood samples were collected on D17 (embryos implanted) for progesterone determination by radioimmunoassay. In experiment 1, average plasma progesterone concentrations after corpora lutea formation (D12 plus D16 means) were 13.7±1.8 ng/ml, 14.5±2.3 ng/ml and 10.8±2.3 ng/ml for control, T800 and T1200, respectively, and did not differ (P=0.44). In experiment 2, progesterone concentrations on D17 in Control (8.88±0.57 ng/ml), T800 (7.48±0.64 ng/ml) and T1200 (5.90±1.33 ng/ml) groups were similar (P=0.11). Results indicate that the supplemental betacarotene and tocopherol injections did not influence peripheral progesterone concentrations in superovulated Holstein donors and crossbreed recipients heifers. Dois experimentos foram conduzidos para avaliar o efeito da injeção intramuscular de betacaroteno associada ao tocoferol, na concentração plasmática de progesterona de novilhas Holandesas superovuladas (Experimento 1) e em novilhas cruzadas (Bos taurus x Bos indicus) submetidas à transferência embrionária em tempo fixo (TETF, experimento 2). No experimento 1, após a sincronização do estro e superovulação, os animais foram inseminados entre 12 a 24 h após o início do estro e os embriões recuperados após 7 dias. As novilhas foram divididas, aleatoriamente, em 1 de 3 tratamentos: Controle (duas injeções de soro); T800 (800 mg de betacaroteno mais 500 mg de tocoferol) e T1200 (1.200 mg de betacaroteno mais 750 mg de tocoferol). Os tratamentos foram administrados no dia da colocação do implante de progesterona e repetido no primeiro dia da superovulação. As colheitas de sangue foram realizadas no D0, D5, D9, D12 e D16. No experimento 2, os tratamentos foram realizados no momento da inserção do dispositivo de progesterona (D0). Utilizou-se o mesmo delineamento do experimento 1. A colheita de sangue para a determinação da progesterona por radioimunoensaio foi realizada no D17 (inovulação embrionária). No experimento 1, a concentração plasmática média, após formação do corpo lúteo (média da soma de D12 com D16) foi de 13,7±1,8 ng/ml, 14,5±2,3 ng/ml e 10,8±2,3 ng/ml para os grupos Controle, T800 e T1200, respectivamente, e não diferiram (P=0,44) entre os tratamentos. No experimento 2, a concentração de progesterona nas receptoras, no dia 17, foi semelhante (P=0,11) entre os grupos controle (8,88±0,57 ng/ml), T800 (7,48±0,64 ng/ml) e T1200 (5,90±1,33 ng/ml). Os resultados indicaram que as injeções de suplemento com betacaroteno e tocoferol não influenciaram na concentração plasmática de progesterona em novilhas Holandesas doadoras de embriões e em novilhas cruzadas, receptoras de embrião.

Published
2011

29. Actions of angiotensin II and dopamine in the medial preoptic area on prolactin secretion

Author

Leite, C. M., Machado, G. J.R. [UNESP], Dornelles, R. C.M. [UNESP], Franci, Celso Rodrigues, Universidade Estadual Paulista (UNESP), and Universidade de São Paulo (USP)

Subjects
Dopaminergic antagonists, Angiotensin II, Medial preoptic area, Estrogen, Prolactin
Abstract

Made available in DSpace on 2022-04-28T20:41:58Z (GMT). No. of bitstreams: 0 Previous issue date: 2008-06-24 Dopamine (DA) is known as a primary regulator of prolactin secretion (PRL) and angiotensin II (Ang II) has been recognized as one brain inhibitory factor of this secretion. In this work, estrogen-primed or unprimed ovariectomized rats were submitted to the microinjection of saline or Ang II after previous microinjection of saline or of DA antagonist (haloperidol, sulpiride or SCH) both in the medial preoptic area (MPOA). Our study of these interactions has shown that 1) estrogen-induced PRL secretion is mediated by Ang II and DA actions in the MPOA, i.e. very high plasma PRL would be prevented by inhibitory action of Ang II, while very low levels would be prevented in part by stimulatory action of DA through D2 receptors, 2) the inhibitory action of Ang II depends on estrogen and is mediated in part by inhibitory action of DA through D1 receptors and in other part by inhibition of stimulatory action of DA through D2 receptors. © 2008 Institute of Physiology v.v.i., Academy of Sciences of the Czech Republic. Department of Basic Science Faculty of Odontology University of Estadual Paulista, Araçatuba Department of Physiology Faculty of Medicine of Ribeirão Preto University of São Paulo, Av. Bandeirantes, Ribeirão Preto Departamento de Fisiologia Faculdade de Medicina de Ribeirão Preto Campus USP, Av. Bandeirantes, 3900, 14049-900, Ribeirão Preto, SP Department of Basic Science Faculty of Odontology University of Estadual Paulista, Araçatuba

Published
2008

32. Corticosterone plasma concentrations in Carioca High- and Low-Conditioned freezing rats after a fear conditioned task

Author

Leon A., Laura Andrea, Castro Gomes, Vitor, Brandao, Marcus Lira, Franci, Celso Rodrigues, Cardenas P., Fernando, Landeira-Fernandez, Jesus, Leon A., Laura Andrea, Castro Gomes, Vitor, Brandao, Marcus Lira, Franci, Celso Rodrigues, Cardenas P., Fernando, and Landeira-Fernandez, Jesus

Abstract

Our group in the Psychology Department at Pontifícia Universidade Católica do Rio de Janeiro (PUC-Rio) developed a rat genetic model of extreme freezing in response to contextual cues in an experimental chamber previously associated with footshock. One of the lines, Carioca High Freezing (CHF), exhibits an enhanced conditioned freezing response, whereas the other line, Carioca Low Freezing (CLF), shows the opposite response. The present study investigated corticosterone concentration between these two lines of animals and a random (RND) line of rats both under basal conditions and test condition after an emotional challenge using a contextual fear conditioning protocol. Comparisons between basal and test plasma corticosterone concentrations suggested differential basal and fear-induced differences between the two lines. The differences between basal conditions is an important and relevant aspect to be considered in behavioral experiments using or assessing stress and could help to understand variability in naïve populations., Casi toda la investigación farmacológica en estrés y desórdenes de ansiedad es realizada generalmente en poblaciones de animales que se supone son comparables. En el laboratorio de Neurociencia Comportamental de la Pontificia Universidad Católica de Río de Janeiro (Brasil), dos nuevas líneas de ratas Wistar fueron aisladas por la selección fenotípica de la respuesta emocional en el protocolo de condicionamiento de la respuesta de miedo. Una de las líneas, denominada Carioca High Freezing (CHF) muestra una respuesta aumentada de congelamiento en el test, mientras que la otra –Carioca Low Freezing (CLF)– muestra la respuesta opuesta. Aquí presentamos datos orientados a evaluar las condiciones basales y la vulnerabilidad al estrés entre las dos líneas. Los niveles de corticosterona fueron comparados entre las dos líneas tanto en condiciones basales como después de un desafío emocional utilizando el protocolo de miedo condicionado. La comparación entre las concentraciones plasmáticas de corticosterona basal y luego del retest sugiere diferencias basales y diferencias inducidas por el miedo entre las dos líneas. Las diferencias en las condiciones basales es un aspecto importante y relevante que debe ser considerado en experimentos comportamentales que usen o evalúen el estrés y podría ayudar a comprender la variabilidad encontrada en las poblaciones., Nosso grupo no Departamento de Psicologia da Universidade Católica do Rio de Janeiro desenvolveu um modelo genético com ratos que apresentam respostas extremas de congelamento a estímulos contextuais de uma caixa experimental previamente associados a choques elétricos. Uma das linhagens, Carioca High Freezing (CHF) apresenta uma resposta aumentada de congelamento condicionado. A outra linhagem, Carioca Low Freezing (CLF) apresenta a resposta de congelamento condicionada em direção oposta. O presente trabalho comparou os níveis de corticosterona entre as duas linhagens assim como uma terceira linhagem com cruzamento aleatório (RND) tanto em condições basais assim como em um teste onde os animais foram expostos á situação de condicionamento contextual de medo. A comparação entre as concentrações plasmáticas de corticosterona basal e depois do teste sugere diferenças basais assim como diferenças induzidas pelo medo condicionado entre as duas linhagens. As diferenças nas condições basais é um aspecto importante e relevante que deve ser levado em consideração em experimentos que avaliem o estresse e pode ajudar na compreensão da variabilidade nas populações.

Published
2013

39. Neonatal Handling Reduces Renal Function in Adult Rats

Author

Donadio, Marcio Vinicius Fagundes, primary, Jacobs, Silvana, additional, Corezola, Kizzy Ludnila, additional, da Silva Melo, Denizar Alberto, additional, Bregolin Dias, Henrique, additional, Reichel, Carlos Luiz, additional, Franci, Celso Rodrigues, additional, Jeckel-Neto, Emilio Antonio, additional, Lulhier, Francisco, additional, de Oliveira, Jarbas Rodrigues, additional, and Sanvitto, Gilberto Luiz, additional

Published
2009
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40. Effects of Neonatal Handling on Central Noradrenergic and Nitric Oxidergic Systems and Reproductive Parameters in Female Rats

Author

Raineki, Charlis, primary, Szawka, Raphael Escorsim, additional, Gomes, Cármen Marilei, additional, Lucion, Marta Knijnik, additional, Barp, Jaqueline, additional, Belló-Klein, Adriane, additional, Franci, Celso Rodrigues, additional, Anselmo-Franci, Janete Aparecida, additional, Sanvitto, Gilberto Luiz, additional, and Lucion, Aldo Bolten, additional

Published
2007
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