1. Determination of Larval Instars of Dastarcus helophoroides (Coleoptera: Bothrideridae) Using Head Capsule Width Frequency Distribution.
- Author
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Shaheen, Tayyab, Guo, Jiali, Wang, Yun, Zhou, Jiaojiao, Tang, Guanghui, and Zhang, Zhengqing
- Subjects
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CERAMBYCIDAE , *DISTRIBUTION (Probability theory) , *INSECT populations , *PEST control , *LIFE tables - Abstract
Simple Summary: Long-horned beetles can cause severe damage to forest plantations and disrupt forest ecosystems. Controlling their populations is essential for maintaining healthy forests. Biological control, as an efficient and environmentally friendly pest management strategy, can be applied in the early stages of pest development. Laboratory-reared Dastarcus helophoroides released into woodlands has proven effective in the sustainable control of long-horned beetle populations. However, the larval instars of D. helophoroides have not yet been defined, which complicates field identification. In this study, we measured the head capsule widths of D. helophoroides larval instars, visually inspected the histogram, and performed non-linear least squares estimation. Our findings revealed that D. helophoroides passes through four larval instars before reaching the pupal stage. Accurately determining the larval instars of a parasitoid is essential for developing effective management programs and predicting future abundances in the field. The findings of this study will be instrumental in implementing biological control strategies for the effective management of long-horned beetles. Long-horned beetles are among the major insect pests that can cause significant economic and ecological damage globally. The control of long-horned beetles is crucial to sustain the forest ecosystem. Dastarcus helophoroides, an economically important ectoparasitoid of long-horned beetles, is widely utilized in biological control strategies. However, the number of larval instars in D. helophoroides remains underexplored. Larval instar determination is crucial for constructing growth prediction models and ecological life tables for insect populations. In this study, we analyzed the frequency distribution of head capsule widths utilizing a visual approach, followed by a non-linear least squares (NLLS) estimation, and found that D. helophoroides undergo four larval instars before entering the pupal stage. The theoretical and observed data for each larval instar yielded identical mean Brooks–Dyar's ratios (1.80). Re-correlation of the number of instars with their respective mean head capsule widths using linear regression (R²) verified that no larval instar was missed. The Crosby's growth ratio (1% and 2%) indicates a very low likelihood of misclassifying an instar into an adjacent one. Given that the accurate determination of larval instars is crucial for developing effective control programs and predicting future population levels, our findings provide valuable insights for implementing biological control strategies against long-horned beetles. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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