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3. Structure and Oligonucleotide Binding Efficiency of Differently Prepared Click Chemistry-Type DNA Microarray Slides Based on 3-Azidopropyltrimethoxysilane

Author

Frydrych-Tomczak, Emilia, primary, Ratajczak, Tomasz, additional, Kościński, Łukasz, additional, Ranecka, Agnieszka, additional, Michalak, Natalia, additional, Luciński, Tadeusz, additional, Maciejewski, Hieronim, additional, Jurga, Stefan, additional, Lewandowski, Mikołaj, additional, and Chmielewski, Marcin K., additional

Published
2021
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10. The "Clickable" Method for Oligonucleotide Immobilization Onto Azide-Functionalized Microarrays.

Author

Ratajczak T, Uszczyńska B, Frydrych-Tomczak E, and Chmielewski MK

Subjects
Ascorbic Acid chemistry, Copper Sulfate chemistry, Glass chemistry, Glycerol chemistry, Nucleic Acid Hybridization, Oligodeoxyribonucleotides chemical synthesis, Oligodeoxyribonucleotides chemistry, Silanes chemistry, Azides chemistry, Click Chemistry methods, Oligonucleotide Array Sequence Analysis methods, Oligonucleotides chemistry
Abstract

The DNA microarray technique was supposed to help identifying and analyzing the expression level of tens of thousands of genes in the whole genome. But there is a serious problem concerning fabrication of the microarrays by chemical synthesis, such as specific and efficient linking of probes to a solid support. Therefore, we reckon that applying "click" chemistry to covalently anchor oligonucleotides on chemically modified supports may help construct microarrays in applications such as gene identification. Silanization of the glass support with organofunctional silane makes it possible to link azide groups on glass surface and the nucleic acid probe that is equipped with a pentynyl group. This is followed by direct spotting of the nucleic acid on the azide-modified glass support in the presence of copper ions, and this is a frequently applied method of "click" chemistry.

Published
2016
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