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The process of bone regeneration is complicated, and it is still a major clinical challenge to regenerate critical-size bone defects caused by severe trauma, infection, and tumor resection. Intracellular metabolism has been found to play an important role in the cell fate decision of skeletal progenitor cells. GW9508, a potent agonist of the free fatty acid receptors GPR40 and GPR120, appears to have a dual effect of inhibiting osteoclastogenesis and promoting osteogenesis by regulating intracellular metabolism. Hence, in this study, GW9508 was loaded on a scaffold based on biomimetic construction principles to facilitate the bone regeneration process. Through 3D printing and ion crosslinking, hybrid inorganic-organic implantation scaffolds were obtained after integrating 3D-printed β-TCP/CaSiO3 scaffolds with a Col/Alg/HA hydrogel. The 3D-printed β-TCP/CaSiO3 scaffolds had an interconnected porous structure that simulated the porous structure and mineral microenvironment of bone, and the hydrogel network shared similar physicochemical properties with the extracellular matrix. The final osteogenic complex was obtained after GW9508 was loaded into the hybrid inorganic-organic scaffold. To investigate the biological effects of the obtained osteogenic complex, in vitro studies and a rat cranial critical-size bone defect model were utilized. Metabolomics analysis was conducted to explore the preliminary mechanism. The results showed that 50 μM GW9508 facilitated osteogenic differentiation by upregulating osteogenic genes, including Alp, Runx2, Osterix, and Spp1 in vitro. The GW9508-loaded osteogenic complex enhanced osteogenic protein secretion and facilitated new bone formation in vivo. Finally, the results from metabolomics analysis suggested that GW9508 promoted stem cell differentiation and bone formation through multiple intracellular metabolism pathways, including purine and pyrimidine metabolism, amino acid metabolism, glutathione metabolism, and taurine and hypotaurine metabolism. This study provides a new approach to address the challenge of critical-size bone defects. [ABSTRACT FROM AUTHOR]

In laser-induced thermal-crack propagation, thermal stresses control the crack propagation mode and the quality of the crack surface. In this article, the 1064-nm semiconductor laser as a volumetric heat source is used for symmetry and asymmetry cutting glass. One of the problems in laser asymmetry linear cutting glass with laser-induced thermal-crack propagation is the quality of the crack surface which gets worse than that in symmetry cutting. This study lays great emphasis on analyzing the effect of the focus position and shear stresses on the crack sectional shape in semiconductor laser asymmetry linear cutting glass. This article indicates the volumetric heat flux formula, which simulates the temperature distribution from the material above or below the focal point. The heat source should be positioned above the focal point. Optical microscope photographs of the crack surface and sectional shape are obtained to examine the surface quality which is explained from the results of the stress fields using the extended finite element method simulation. In asymmetry cutting, shear stress is parallel to the crack surface and perpendicular to the cutting direction, which makes the crack surface smooth but uneven. [ABSTRACT FROM AUTHOR]