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Micro-X is an X-ray sounding rocket payload that had its first flight on July 22, 2018. The goals of the first flight were to operate a transition edge sensor (TES) X-ray microcalorimeter array in space and take a high-resolution spectrum of the Cassiopeia A supernova remnant. The first flight was considered a partial success. The array and its time-division multiplexing readout system were successfully operated in space, but due to a failure in the attitude control system, no time on-target was acquired. A re-flight has been scheduled for summer 2022. Since the first flight, modifications have been made to the detector systems to improve noise and reduce the susceptibility to magnetic fields. The three-stage SQUID circuit, NIST MUX06a, has been replaced by a two-stage SQUID circuit, NIST MUX18b. The initial laboratory results for the new detector system will be presented in this paper., Comment: LTD proceedings, 6 pages, 3 figures, 1 table

The Micro-X sounding rocket is a NASA funded X-ray telescope payload that completed its first flight on July 22, 2018. This event marked the first operation of Transition Edge Sensors (TESs) and their SQUID-based multiplexing readout system in space. Unfortunately, due to an ACS pointing failure, the rocket was spinning during its five minute observation period and no scientific data was collected. However, data collected from the internal calibration source marked a partial success for the payload and offers a unique opportunity to study the response of TESs and SQUIDs in space. Of particular interest is the magnetic field response of the NIST MUX06a SQUID readout system to tumbling through Earth's magnetic field. We present a model to explain the baseline response of the SQUIDs, which lead to a subset of pixels failing to "lock" for the full observational period. Future flights of the Micro-X rocket will include the NIST MUX18b SQUID system with dramatically reduced magnetic susceptibility., Comment: 6 pages, 6 figures, LTD19 conference proceedings

With its first flight in 2018, Micro-X became the first program to fly Transition-Edge Sensors and their SQUID readouts in space. The science goal was a high-resolution, spatially resolved X-ray spectrum of the Cassiopeia A Supernova Remnant. While a rocket pointing error led to no time on target, the data was used to demonstrate the flight performance of the instrument. The detectors observed X-rays from the on-board calibration source, but a susceptibility to external magnetic fields limited their livetime. Accounting for this, no change was observed in detector response between ground operation and flight operation. This paper provides an overview of the first flight performance and focuses on the upgrades made in preparation for reflight. The largest changes have been upgrading the SQUIDs to mitigate magnetic susceptibility, synchronizing the clocks on the digital electronics to minimize beat frequencies, and replacing the mounts between the cryostat and the rocket skin to improve mechanical integrity. As the first flight performance was consistent with performance on the ground, reaching the instrument goals in the laboratory is considered a strong predictor of future flight performance.

Both culture-based and culture-independent, biomarker-targeted microbial enumeration and identification technologies were employed to estimate total microbial and viral burden and diversity within the cabin air of commercial airliners. Samples from each of twenty flights spanning three commercial carriers were collected via air-impingement. When the total viable microbial population was estimated by assaying relative concentrations of the universal energy carrier ATP, values ranged from below detection limits (BDL) to 4.1 x 106 cells/cubic m of air. The total viable microbial population was extremely low in both of Airline A (approximately 10% samples) and C (approximately 18% samples) compared to the samples collected aboard flights on Airline A and B (approximately 70% samples). When samples were collected as a function of time over the course of flights, a gradual accumulation of microbes was observed from the time of passenger boarding through mid-flight, followed by a sharp decline in microbial abundance and viability from the initiation of descent through landing. It is concluded in this study that only 10% of the viable microbes of the cabin air were cultivable and suggested a need to employ state-of-the art molecular assay that measures both cultivable and viable-but-non-cultivable microbes. Among the cultivable bacteria, colonies of Acinetobacter sp. were by far the most profuse in Phase I, and Gram-positive bacteria of the genera Staphylococcus and Bacillus were the most abundant during Phase II. The isolation of the human pathogens Acinetobacter johnsonii, A. calcoaceticus, Janibacter melonis, Microbacterium trichotecenolyticum, Massilia timonae, Staphylococcus saprophyticus, Corynebacterium lipophiloflavum is concerning, as these bacteria can cause meningitis, septicemia, and a handful of sometimes fatal diseases and infections. Molecular microbial community analyses exhibited presence of the alpha-, beta-, gamma-, and delta- proteobacteria, as well as Gram-positive bacteria, Fusobacteria, Cyanobacteria, Deinococci, Bacterioidetes, Spirochetes, and Planctomyces in varying abundance. Neisseria meningitidis rDNA sequences were retrieved in great abundance from Airline A followed by Streptococcus oralis/mitis sequences. Pseudomonas synxantha sequences dominated Airline B clone libraries, followed by those of N. meningitidis and S. oralis/mitis. In Phase II, Airline C, sequences representative of more than 113 species, enveloping 12 classes of bacteria, were retrieved. Proteobacterial sequences were retrieved in greatest frequency (58% of all clone sequences), followed in short order by those stemming from Gram-positives bacteria (31% of all clone sequences). As for overall phylogenetic breadth, Gram-positive and alpha-proteobacteria seem to have a higher affinity for international flights, whereas beta-and gamma-proteobacteria are far more common about domestic cabin air parcels in Airline C samples. Ultimately, the majority of microbial species circulating throughout the cabin airs of commercial airliners are commensal, infrequently pathogenic normal flora of the human nasopharynx and respiratory system. Many of these microbes likely originate from the oral and nasal cavities, and lungs of passengers and flight crew and are disseminated unknowingly via routine conversation, coughing, sneezing, and stochastic passing of fomites. The data documented in this study will be useful to generate a baseline microbial population database and can be utilized to develop biosensor instrumentation for monitoring microbial quality of cabin or urban air.

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