1. Role of the chemokines CCL3/MIP-1 α and CCL5/RANTES in sponge-induced inflammatory angiogenesis in mice
- Author
-
Mauro M. Teixeira, Lucíola S. Barcelos, Guilherme Bruno-Lima, Silvia Passos Andrade, Adriano L.S. Souza, Rodrigo Guabiraba, Remo Castro Russo, Amanda E. I. Proudfoot, Amanda M. Coelho, Universidade Federal de Minas Gerais, Infectiologie Animale et Santé Publique (UR IASP), Institut National de la Recherche Agronomique (INRA), Merck Serono, Merck & Co. Inc, Conselho Nacional de Desenvolvimento Cientí fi co e Tecnológico (CNPq/Brasil), Fundação do Amparo a Pesquisas do Estado de Minas Gerais (FAPEMIG), and Union FP6 (INNOCHEM, Grant number LSHB-CT-2005-518167)
- Subjects
Male ,Surgical Sponges ,medicine.medical_specialty ,Chemokine ,Time Factors ,Angiogenesis ,Neovascularization, Physiologic ,CCL3 ,Inflammation ,chemokines ,Biochemistry ,Neovascularization ,Mice ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,parasitic diseases ,medicine ,Animals ,Macrophage ,Chemokine CCL5 ,Chemokine CCL3 ,030304 developmental biology ,Mice, Knockout ,0303 health sciences ,Dose-Response Relationship, Drug ,biology ,Chemistry ,Met-RANTES ,virus diseases ,hemic and immune systems ,Cell Biology ,respiratory system ,Recombinant Proteins ,3. Good health ,Mice, Inbred C57BL ,Endocrinology ,medicine.anatomical_structure ,inflammation ,030220 oncology & carcinogenesis ,Myeloperoxidase ,biology.protein ,[PHYS.PHYS.PHYS-MED-PH]Physics [physics]/Physics [physics]/Medical Physics [physics.med-ph] ,[SDV.IMM]Life Sciences [q-bio]/Immunology ,medicine.symptom ,neovascularization ,Cardiology and Cardiovascular Medicine ,Blood vessel - Abstract
Barcelos, Luciola S Coelho, Amanda M Russo, Remo C Guabiraba, Rodrigo Souza, Adriano L S Bruno-Lima, Guilherme Jr Proudfoot, Amanda E I Andrade, Silvia P Teixeira, Mauro M Microvasc Res. 2009 Sep;78(2):148-54. doi: 10.1016/j.mvr.2009.04.009. Epub 2009 May 8.; International audience; OBJECTIVE: We examined the potential contribution of CCL3 and CCL5 to inflammatory angiogenesis in mice. METHODS: Polyester-polyurethane sponges were implanted in mice and blood vessel counting and hemoglobin, myeloperoxidase and N-acetylglucosaminidase measurements used as indexes for vascularization, neutrophil and macrophage accumulation, respectively. RESULTS: CCL3 and CCL5 were expressed throughout the observation period. Exogenous CCL3 enhanced angiogenesis in WT, but angiogenesis proceeded normally in CCL3(-/-) mice, suggesting that endogenous CCL3 is not critical for sponge-induced angiogenesis in mice. CCL5 expression was detected at day 1, but levels significantly increased thereafter. Exogenous CCL5 reduced angiogenesis in WT mice possible via CCR5 as CCL5 was without an effect in CCR5(-/-) mice. Treatment of WT with the CCR1/CCR5 antagonist, Met-RANTES, prevented neutrophil and macrophage accumulation, but enhanced sponge vascularization. CONCLUSION: Thus, endogenous CCL3 appears not to play a role in driving sponge-induced inflammatory angiogenesis in mice. The effects of CCL5 were anti-angiogenic and appeared to be mediated via activation of CCR5.
- Published
- 2009
- Full Text
- View/download PDF