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38. Differentiation of Tumor Cells : Induction of Erythrocyte Membrane-Specific Antigens in Friend Leukemia Cells

Author

Sugano, H., Furusawa, M., Kawaguchi, T., Ikawa, Y., Allfrey, V. G., editor, Allgöwer, M., editor, Bauer, K. H., editor, Berenblum, I., editor, Bergel, F., editor, Bernard, J., editor, Bernhard, W., editor, Blokhin, N. N., editor, Bock, H. E., editor, Braun, W., editor, Bucalossi, P., editor, Chaklin, A. V., editor, Chorazy, M., editor, Cunningham, G. J., editor, Dargent, M., editor, Della Porta, G., editor, Denoix, P., editor, Dulbecco, R., editor, Eagle, H., editor, Eker, R., editor, Good, R. A., editor, Grabar, P., editor, Hamperl, H., editor, Harris, R. J. C., editor, Hecker, E., editor, Herbeuval, R., editor, Higginson, J., editor, Hueper, W. C., editor, Isliker, H., editor, Kieler, J., editor, Klein, G., editor, Koprowski, H., editor, Koss, L. G., editor, Martz, G., editor, Mathé, G., editor, Mühlbock, O., editor, Nakahara, W., editor, Old, L. J., editor, Potter, V. R., editor, Sabin, A. B., editor, Sachs, L., editor, Saxén, E. A., editor, Schmidt, C. G., editor, Spiegelman, S., editor, Szybalski, W., editor, Tagnon, H., editor, Taylor, R. M., editor, Tissières, A., editor, Uehlinger, E., editor, Wissler, R. W., editor, Yoshida, T., editor, Rentchnick, P., editor, and Grundmann, E., editor

Published
1973
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48. Alkaline extracellular conditions promote the proliferation and mineralization of a human cementoblast cell line.

Author

Muramatsu, T., Kashiwagi, S., Ishizuka, H., Matsuura, Y., Furusawa, M., Kimura, M., and Shibukawa, Y.

Subjects
CELL proliferation, ALKALINE phosphatase regulation, BIOMINERALIZATION, CEMENTUM, EXTRACELLULAR enzymes, ENZYME activation, ALIZARIN, ANKYRINS
Abstract

Aim: To investigate the proliferation and mineralization of a human cementoblast cell line under alkaline conditions. Methodology: A human cementoblast cell line was cultured in alkaline media with several pHs (pH 7.6, 8.0 and 8.4) without CO2. Cell numbers, phospho‐p44/42 expression, alkaline phosphatase (ALP) activity and mineralization were evaluated. The significance of differences between groups was assessed using two‐way analysis of variance 15 (ANOVA) followed by Bonferroni's multiple comparison test (α = 0.01). Results: Cell numbers increased in a time‐dependent manner in the high pH medium groups. Western blot analysis revealed the upregulated expression of phospho‐p44/42 under alkaline conditions. ALP activity was also increased at pH 8.0 and 8.4. Alizarin red staining revealed increased mineralization in the high pH medium groups. The incorporation of the transient receptor potential ankyrin subfamily member 1 (TRPA1) antagonist HC030031 markedly negated the effect on proliferation and mineralization. Conclusions: Extracellular alkaline conditions promoted the proliferation and mineralization of human cementoblasts in vitro via TRPA1. [ABSTRACT FROM AUTHOR]

Published
2019
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49. Use of Erythrocyte Ghosts for Therapy

Author

Furusawa, M., Iino, T., Heimpel, H., editor, Huhn, D., editor, Mueller-Eckhardt, C., editor, Ruhenstroth-Bauer, G., editor, Neth, Rolf, editor, Gallo, Robert C., editor, Graf, Thomas, editor, Mannweiler, Klaus, editor, and Winkler, Kurt, editor

Published
1981
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