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146 results on '"Furuta, Ken'ya"'

1. Gliding filament system giving both orientational order and clusters in collective motion

Author

Tanida, Sakurako, Furuta, Ken'ya, Nishikawa, Kaori, Hiraiwa, Tetsuya, Kojima, Hiroaki, Oiwa, Kazuhiro, and Sano, Masaki

Subjects
Condensed Matter - Soft Condensed Matter
Abstract

Active matter consists of self-propelled elements exhibits fascinating collective motions ranging from biological to artificial systems. Among wide varieties of active matter systems, reconstituted bio-filaments moving on molecular motor turf interacting purely by physical interactions provides the fundamental test ground for understanding biological motility. However, until now, multi-filament collisions,depletion agents or binding molecules has been required for the emergence of ordered patterns in motility assay. Thus, whether simple physical interactions during collisions such as steric effect without depletion nor binding agents are sufficient or not for producing ordered patterns in motility assays remains still elusive. In this article, we constructed a motility assay purely consists of kinesin motor and microtubule in which the frequency of binary collision can be controlled without using depletion nor binding agents. By controlling strength of steric interaction and density of microtubules, we found different states; disordered state, long-range orientationally ordered state, liquid-gas-like phase separated state, and transitions between them. We found that a balance between cross over and aligning events in collisions controls transition from disorder to global ordered state, while excessively strong steric effect leads to the phase separated clusters. Furthermore, macroscopic chiral symmetry breaking observed as a global rotation of nematic order observed in this experiment could be attributed to the chirality at molecular level. Numerical simulations in which we change strength of volume exclusion reproduce these experimental results. Moreover, it reveals the transition from long-range alignment to nematic bands then to aggregations. This study may provide new insights into dynamic ordering by self-propelled elements through a purely physical interaction., Comment: 18 pages, 11 figures

Published
2018
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11. List of Contributors

Author

Avery, Adam W., primary, Chan, Gordon K., additional, Dean, Anudariya B., additional, Desai, Paurav B., additional, Gibbons, Ian R., additional, Hays, Thomas S., additional, Hinchcliffe, Edward H., additional, Hou, Yuqing, additional, Hunter, Emily L., additional, Hwang, Juyeon, additional, Ishikawa, Takashi, additional, Jha, Rupam, additional, Kamiya, Ritsu, additional, Ketcham, Stephanie A., additional, Kevin Pfister, K., additional, King, Stephen M., additional, Lewis, Cody W., additional, Li, Min-Gang, additional, McKenney, Richard J., additional, Mitchell, David R., additional, Neisch, Amanda L., additional, Porter, Mary E., additional, Sale, Winfield S., additional, Schroer, Trina A., additional, Shingyoji, Chikako, additional, Surrey, Thomas, additional, Ueki, Noriko, additional, Vallee, Richard B., additional, Vaughan, Kevin T., additional, Wakabayashi, Ken-ichi, additional, Wickstead, Bill, additional, Wirschell, Maureen, additional, Witman, George B., additional, Xiang, Xin, additional, Yagi, Toshiki, additional, Berthoux, Lionel, additional, Carter, Andrew P., additional, Dawn, Amrita, additional, Doobin, David J., additional, Fainzilber, Mike, additional, Fisher, Elizabeth M.C., additional, Furuta, Ken’ya, additional, Geyer, Veikko F., additional, Gross, Steven P., additional, Hancock, William O., additional, Hill, Kent L., additional, Holzbaur, Erika L.F., additional, Howard, Jonathon, additional, Imhof, Simon, additional, Inaba, Kazuo, additional, Jülicher, Frank, additional, Kapoor, Tarun M., additional, Koley, Sandip, additional, Lightcap, Christine M., additional, Loges, Niki T., additional, Milev, Miroslav P., additional, Mitchison, Hannah M., additional, Moughamian, Armen J., additional, Mouland, Andrew J., additional, Oiwa, Kazuhiro, additional, Omran, Heymut, additional, Pilder, Stephen H., additional, Price, Michael, additional, Reddy, Babu J.N., additional, Sakakibara, Hitoshi, additional, Sartori, Pablo, additional, Schmidt, Helgo, additional, Schmidts, Miriam, additional, Siglin, Amanda E., additional, Sisson, Joseph H., additional, Steinman, Jonathan B., additional, Shubeita, George T., additional, Terenzio, Marco, additional, Vincent, John B., additional, Williams, John C., additional, Yang, Fan, additional, Yao, Xaojian, additional, and Yildiz, Ahmet, additional

Published
2018
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13. Dynamic Control of Microbial Movement by Photoswitchable ATP Antagonists

Author

Thayyil, Sampreeth, Nishigami, Yukinori, Islam, Md Jahirul, Hashim, P. K., Furuta, Ken'ya, Oiwa, Kazuhiro, Yu, Jian, Yao, Min, Nakagaki, Toshiyuki, 1000000344218, Tamaoki, Nobuyuki, Thayyil, Sampreeth, Nishigami, Yukinori, Islam, Md Jahirul, Hashim, P. K., Furuta, Ken'ya, Oiwa, Kazuhiro, Yu, Jian, Yao, Min, Nakagaki, Toshiyuki, 1000000344218, and Tamaoki, Nobuyuki

Abstract

Adenosine triphosphate (ATP) is the energy source for various biochemical processes and biomolecular motors in living things. Development of ATP antagonists and their stimuli-controlled actions offer a novel approach to regulate biological processes. Herein, we developed azobenzene-based photoswitchable ATP antagonists for controlling the activity of motor proteins; cytoplasmic and axonemal dyneins. The new ATP antagonists showed reversible photoswitching of cytoplasmic dynein activity in an in vitro dynein-microtubule system due to the trans and cis photoisomerization of their azobenzene segment. Importantly, our ATP antagonists reversibly regulated the axonemal dynein motor activity for the force generation in a demembranated model of Chlamydomonas reinhardtii. We found that the trans and cis isomers of ATP antagonists significantly differ in their affinity to the ATP binding site.

Published
2022

19. Planar cell polarity induces local microtubule bundling for coordinated ciliary beating

Author

Nakayama, Shogo, primary, Yano, Tomoki, additional, Namba, Toshinori, additional, Konishi, Satoshi, additional, Takagishi, Maki, additional, Herawati, Elisa, additional, Nishida, Tomoki, additional, Imoto, Yasuo, additional, Ishihara, Shuji, additional, Takahashi, Masahide, additional, Furuta, Ken’ya, additional, Oiwa, Kazuhiro, additional, Tamura, Atsushi, additional, and Tsukita, Sachiko, additional

Published
2021
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21. Different motilities of microtubules driven by kinesin-1 and kinesin-14 motors patterned on nanopillars

Author

80448050, 10411216, Kaneko, Taikopaul, Furuta, Ken’ya, Oiwa, Kazuhiro, Shintaku, Hirofumi, Kotera, Hidetoshi, Yokokawa, Ryuji, 80448050, 10411216, Kaneko, Taikopaul, Furuta, Ken’ya, Oiwa, Kazuhiro, Shintaku, Hirofumi, Kotera, Hidetoshi, and Yokokawa, Ryuji

Abstract

Kinesin is a motor protein that plays important roles in a variety of cellular functions. In vivo, multiple kinesin molecules are bound to cargo and work as a team to produce larger forces or higher speeds than a single kinesin. However, the coordination of kinesins remains poorly understood because of the experimental difficulty in controlling the number and arrangement of kinesins, which are considered to affect their coordination. Here, we report that both the number and spacing significantly influence the velocity of microtubules driven by nonprocessive kinesin-14 (Ncd), whereas neither the number nor the spacing changes the velocity in the case of highly processive kinesin-1. This result was realized by the optimum nanopatterning method of kinesins that enables immobilization of a single kinesin on a nanopillar. Our proposed method enables us to study the individual effects of the number and spacing of motors on the collective dynamics of multiple motors.

Published
2020

28. Katanin p80, NuMA and cytoplasmic dynein cooperate to control microtubule dynamics

Author

Jin, Mingyue, Pomp, Oz, Shinoda, Tomoyasu, Toba, Shiori, Torisawa, Takayuki, Furuta, Ken’ya, Oiwa, Kazuhiro, Yasunaga, Takuo, Kitagawa, Daiju, Matsumura, Shigeru, Miyata, Takaki, Tan, Thong Teck, Reversade, Bruno, Hirotsune, Shinji, Jin, Mingyue, Pomp, Oz, Shinoda, Tomoyasu, Toba, Shiori, Torisawa, Takayuki, Furuta, Ken’ya, Oiwa, Kazuhiro, Yasunaga, Takuo, Kitagawa, Daiju, Matsumura, Shigeru, Miyata, Takaki, Tan, Thong Teck, Reversade, Bruno, and Hirotsune, Shinji

Abstract

Human mutations in KATNB1 (p80) cause severe congenital cortical malformations, which encompass the clinical features of both microcephaly and lissencephaly. Although p80 plays critical roles during brain development, the underlying mechanisms remain predominately unknown. Here, we demonstrate that p80 regulates microtubule (MT) remodeling in combination with NuMA (nuclear mitotic apparatus protein) and cytoplasmic dynein. We show that p80 shuttles between the nucleus and spindle pole in synchrony with the cell cycle. Interestingly, this striking feature is shared with NuMA. Importantly, p80 is essential for aster formation and maintenance in vitro. siRNA-mediated depletion of p80 and/or NuMA induced abnormal mitotic phenotypes in cultured mouse embryonic fibroblasts and aberrant neurogenesis and neuronal migration in the mouse embryonic brain. Importantly, these results were confirmed in p80-mutant harboring patient-derived induced pluripotent stem cells and brain organoids. Taken together, our findings provide valuable insights into the pathogenesis of severe microlissencephaly, in which p80 and NuMA delineate a common pathway for neurogenesis and neuronal migration via MT organization at the centrosome/spindle pole.

Published
2017

30. Katanin p80, NuMA and cytoplasmic dynein cooperate to control microtubule dynamics

Author

Jin, Mingyue, primary, Pomp, Oz, additional, Shinoda, Tomoyasu, additional, Toba, Shiori, additional, Torisawa, Takayuki, additional, Furuta, Ken’ya, additional, Oiwa, Kazuhiro, additional, Yasunaga, Takuo, additional, Kitagawa, Daiju, additional, Matsumura, Shigeru, additional, Miyata, Takaki, additional, Tan, Thong Teck, additional, Reversade, Bruno, additional, and Hirotsune, Shinji, additional

Published
2017
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49. Creating biomolecular motors based on dynein and actin-binding proteins

Author

Furuta, Akane, Amino, Misako, Yoshio, Maki, Oiwa, Kazuhiro, Kojima, Hiroaki, and Furuta, Ken'ya

Abstract

Biomolecular motors such as myosin, kinesin and dynein are protein machines that can drive directional movement along cytoskeletal tracks and have the potential to be used as molecule-sized actuators. Although control of the velocity and directionality of biomolecular motors has been achieved, the design and construction of novel biomolecular motors remains a challenge. Here we show that naturally occurring protein building blocks from different cytoskeletal systems can be combined to create a new series of biomolecular motors. We show that the hybrid motors—combinations of a motor core derived from the microtubule-based dynein motor and non-motor actin-binding proteins—robustly drive the sliding movement of an actin filament. Furthermore, the direction of actin movement can be reversed by simply changing the geometric arrangement of these building blocks. Our synthetic strategy provides an approach to fabricating biomolecular machines that work along artificial tracks at nanoscale dimensions.

Published
2017
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