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In many recommendation scenarios, the interactions between users and items are divided into a series of sessions according to the time interval. The traditional Recurrent Neural Network has some shortcomings, such as limited memory ability, inflexible access to memory data, and obvious deficiency in feature capture for long sequences. To deal with the mentioned issues, we propose a hierarchical network with user memory matrix, named HNUM2, which utilizes the memory network to store users' long-term and short-term interests. The memory network is more flexible to access memory data, which can solve the problem of insufficient capture of long sequence features. The proposed model is a hierarchical recommendation algorithm, which consists of two layers. The first layer is the session-level GRU model, which obtains the sequence characteristics of the current session to predict the next item. The second layer is the user-level memory network model which exploits the attention mechanism and incorporates the write module and read module. The experimental results on two public available datasets show that HNUM2 has achieved significant performance improvement comparing to the state-of-the-art methods.

As one of the core framework proteins of nuclear pore complex (NPC), nucleoporin Nupl70 acts as a structural adapter between the nucleolus and nuclear pore membrane and maintains the stability of NPC structure through interaction with other proteins. In this study, we identified a Nup170 protein in the microsporidian Nosema bombycis for the first time and named it as NbNup170. Secondary structure prediction showed that the NbNup170 contains α-helices and random coils. The three-dimensional structure of NbNup170 is elliptical in shape. Phylogenetic analysis based on the Nup170 and homologous sequences showed that N. bombycis clustered together with Vairimorpha ceranae and Vairimorpha apis. The immunofluorescence localization results showed that the NbNup170 was located on the plasma membrane of the dormant spore and transferred to the surface of sporoplasm in a punctate pattern when the dormant spore has finished germination, and that NbNup170 was distributed on the nuclear membrane and both sides of the nuclei of early proliferative phase, and only on the nuclear membrane during sporogonic phase in the N. bombycis. qPCR analysis showed that the relative expression level of NbNup170 maintained at a low level from 30 to 78 h post-infection with N. bombycis, then reached the highest at 102 h, while that of NbNup170 was repressed at a very low level throughout its life cycle by RNA interference. These results suggested that NbNup170 protein is involved in the proliferative phase and active during the sporogonic phase of N. bombycis.

The main function of Sec61 complex is participating in the transport of polypeptide chains across the endoplasmic reticulum. The Sec61α subunit is the largest subunit of the Sec61 complex and shows high degree of conservation. In this study, we identified the NbSec61α and NbSec61γ genes in the microsporidian Nosema bombycis for the first time. Multiple sequence alignment showed that the sequence similarity between NbSec61α and homologous proteins of other microsporidia was greater than 48 %. NbSec61α contains a "plug" domain (amino acids 40-74) unique to the Sec61/SecY complex. Phylogenetic analysis based on NbSec61α and NbSec61γ indicated that the N. bombycis was closely related to Nosema granulosis, Nosema ceranae and Nosema apis. Indirect immunfluorescence assay showed that NbSec61α and NbSec61γ were mainly distributed in the perinuclear region of N. bombycis in different developmental phases. qRT-PCR results revealed that the expression level of NbSec61α gene increased in the early stage and reached the highest at 48 h, then decreased in the late stages. After knockdown of NbSec61α, the expression of NbSec61α, NbSec61γ and NbssrRNA genes were all significantly down-regulated. These results suggest that the NbSec61α and NbSec61γ may play an important role in the intracellular development of N. bombycis.

Background. Bladder cancer (BCa) is one of the most prevalent cancers occurring in the urinary system. Long noncoding RNAs (lncRNAs), in recent years, have emerged as crucial regulators in various biological processes of tumors. Aim. To identify the role of MIR4435-2 host gene (MIR4435-2HG) and uncover its molecular mechanism in BCa. Methods. Firstly, quantitative real-time PCR (RT-qPCR) analysis was used to examine MIR4435-2HG expression in BCa cells. Cell Counting Kit-8 (CCK-8), 5-ethynyl-2 ′ -deoxyuridine (EdU), wound healing, and transwell assays were implemented to identify the role of MIR4435-2HG in BCa. RNA-binding protein immunoprecipitation (RIP), RNA pull down, and luciferase reporter assays were applied to explore the potential mechanism of MIR4435-2HG in BCa. Results. MIR4435-2HG was highly expressed in BCa. Moreover, MIR4435-2HG silencing abrogated BCa cell proliferation, migration, and invasion. In terms of underlying mechanism, MIR44352HG acted as a microRNA-2467-3p (miR-2467-3p) sponge to control the expression of IQ motif containing GTPase activating protein 3 (IQGAP3) and cell division cycle associated 5 (CDCA5), resulting in activation of the rat sarcoma virus (Ras)/rapidly accelerated fibrosarcoma (Raf)/mitogen-activated protein kinase (MEK)/extracellular signal-regulated kinase (ERK) and PI3K/AKT/mTOR signaling pathways. Conclusion. MIR4435-2HG involves in the progression of BCa, which might provide novel insights for BCa treatment.

As a typical species of microsporidium, Nosema bombycis is the pathogen causing the pébrine disease of silkworm. Rapid proliferation of N. bombycis in host cells requires replication of genetic material. As eukaryotic origin recognition protein, origin recognition complex (ORC) plays an important role in regulating DNA replication, and Orc1 is a key subunit of the origin recognition complex. In this study, we identified the Orc1 in the microsporidian N. bombycis (NbOrc1) for the first time. The NbOrc1 gene contains a complete ORF of 987 bp in length that encodes a 328 amino acid polypeptide. Indirect immunofluorescence results showed that NbOrc1 were colocalized with Nbactin and NbSAS-6 in the nuclei of N. bombycis. Subsequently, we further identified the interaction between the NbOrc1 and Nbactin by CO-IP and Western blot. These results imply that Orc1 may be involved in the proliferation of the microsporidian N. bombycis through interacting with actin.

Background: Interstitial cystitis (IC) is a chronic inflammatory disease of the bladder, while both Chemokine (C-X-C motif) ligand 7 (CXCL-7) and Galectin-3 are the molecule related inflammatory diseases.Objective: This study sought to investigate the potential feasibility of diagnosis of interstitial cystitis by measurement of the urinary level of Chemokine (C-X-C motif) ligand 7 (CXCL-7) and Galectin-3.Methods: Both interstitial cystitis patients with or without Hunner’s ulcers were recruited in the study, while healthy subjects were involved as controls. Then we collected urine samples from all the involved subjects, and the CXCL-7 level and Galectin-3 level in the supernatants of urine were measured by ELISA. Urinary creatinine concentrations (mg Cr) of involved subjects were measured to normalize the level of CXCL-7 and Galectin-3. Also the clinical data and demographic characteristics of the recruited subjects were collected. The Receiver-operating curve (ROC) analysis was performed to explore the sensitivity and specialty of both CXCL-7 and Galectin-3 to identify interstitial cystitis. And the associations between urinary levels (CXCL-7 or Galectin-3) and the symptom severity (O'Leary and Sant Symptom, OSSI index) were analyzed with linear regression.Results: The urinary levels of both CXCL-7 and Galectin-3 were significantly increased in interstitial cystitis compared to the healthy controls. The urinary CXCL-7 showed the sensitivity of 59.5% and specificity of 94.6%, at the cut-off value 21.30 pg/mg Cr to discriminate interstitial cystitis from controls, while the urinary Galectin-3 level showed the sensitivity of 62.2% and specificity of 91.9%, at the cut-off value 11.32 pg/mg Cr to discriminate interstitial cystitis from controls. Urinary Galectin-3 level was higher in interstitial cystitis patients with Hunner’s ulcers compared to patients without Hunner’s ulcers. The sensitivity and specificity were 45.5% and 93.3%, respectively for urinary CXCL-7 to discriminate from interstitial cystitis patients with Hunner’s ulcers to patients without Hunner’s ulcers, at the cut-off value of 35.97 pg/mg Cr. In addition, the urinary Galectin-3 level was positive correlated with the symptom severity.Conclusion: CXCL-7 and Galectin-3 were elevated in the interstitial cystitis patients, both of which could be supplementary biomarker for diagnosis of interstitial cystitis. Moreover, urinary CXCL-7 might serve as the biomarker to decimate interstitial cystitis with Hunner ‘s ulcers from the without Hunner’s ulcers.

In this study, by rearranging the typical pyramid lattice structure of carbon fibre reinforced polymer composites to obtain a small node distance and simplify the fabrication procedure, a new type of pyramid lattice structure is designed and fabricated using the snap-fit method. The flatwise compression and bending properties of the new pyramid lattice structure are studied experimentally. The results show that the compression failure mode of the lattice structure is truss fracture, while the bending failure mode is truss fracture and debonding between face sheets and cores. The damage distribution of the fibre and matrix in the lattice structure core is obtained via a finite element simulation, and it is found that there are differences in the ply damage with different fibre directions. In addition, the influence of the core truss length and layering direction on compression and bending properties of the structure are analysed using a finite element simulation.

To accurately reconstruct the three-dimensional (3D) surface of dynamic objects, we proposed a wrapped phase extraction method for spatiotemporal analysis based on 3D wavelet transform (WT). Our proposed method uses a 2D spatial fringe image combined with the time dimension and forms a 3D image sequence. The encoded fringe image sequence’s wrapped phase information was extracted by 3D WT and complex Morlet wavelet, and we improved the wrapped phase extraction’s accuracy by using the characteristics of spatiotemporal analysis and a multi-scale analysis of 3D WT, then we reconstructed the measured object by wrapped phase unwrapping and phase height transformation. Our simulation experiment results show that our proposed method can further filter the noise in the time dimension, and its accuracy is better than that of the one- (1D) and two-dimensional (2D) WT wrapped phase extraction method and the 3D Fourier transform wrapped phase extraction method because the reconstructed spherical crown’s RMSE value does not exceed 0.25 and the PVE value is less than 0.95. Our results show that the proposed method can be applied to the dynamic 3D reconstruction of a real human thoracic and abdominal surface, which fluctuates slowly with respiration movement, further verifying its effectiveness.

Androgen deprivation therapy (ADT) is used to treat prostate cancer (PCa). However, ADT may increase the expression of androgen receptor (AR) through the amplification of chromosome X. The gene oligophrenin 1 (OPHN1) is located in the same region as the AR gene, which could be amplified by ADT. Thus, the role of OPHN1 in PCa pathology was investigated. The expression status of OPHN1 in PCa was searched in The Cancer Genome Atlas (TCGA) database. Androgen‑sensitive cells LNCaP and 22RV1 were cultured under ADT conditions, and then the expression of OPHN1 was evaluated by northern blotting. The expression of OPHN1 was enhanced or knocked down in LNCaP and 22RV1 cells by transfection. Subsequently, the LNCaP and 22RV1 cells were cultured under ADT, and the viability rate, apoptosis, and migration of cells were assessed by MTT, flow cytometry, and Transwell assay respectively. The expression of OPHN1 was also enhanced or knocked down in androgen‑insensitive PC3 cells, and then the effects of OPHN1 on the viability, apoptosis, and migration of PC3 cells were assessed. A mouse xenograft model was created by injecting LNCaP cells with OPHN1 overexpression subcutaneously, and the tumor growth rates were monitored. In TCGA database, amplification of the OPHN1 gene was observed in the PCa tumors. ADT increased the expression of OPHN1 in LNCaP and 22RV1 cells (P

To further improve the hardness of the laser cladding layer on the surface of the vermicular graphite cast iron, the structural parameters of the laser cladding Co-base were designed and optimized, and the properties of the clad layer were evaluated using optical microscopy (OM), scanning electron microscopy (SEM), energy spectroscopy (EDS), X-ray diffractometer (XRD), electrochemical workstation, and friction wear equipment. The results show that the average hardness of the molten layer of Ni and Co-based composite cladding layer is 504 HV0.5, which is 0.64 times that of the Co-based cladding layer due to the combined factors of Ni-Cr-Fe equivalent to the dilution of the Ni-based cladding layer to the Co-based cladding layer. Due to the potential difference of the Ni, Cr, and Co elements on the surface of the cladding layer, the self-corrosion potential of the Ni and Co-based composite cladding layer is 1.08 times that of the Co-based cladding layer, and the self-corrosion current density is 0.51 times. Laser cladding Co-based cladding layer has high corrosion resistance. Under the influence of plastic deformation and oxidative wear of the cladding layer of the Ni and Co-based composite cladding layer, the wear amount of the cladding layer of the Ni and Co-based composite cladding layer is less.

In accordance with the requirement of manufacturing dies quickly and economically, a hybrid forming method of stamping dies for automobile panels is proposed. The method combines digital patternless casting and high-power laser cladding. An experimental study is conducted on the hybrid forming process and its trial production and application in the manufacturing of stamping dies for typical panels. Results prove that the laser cladding layer exceeds HRC60 (Rockwell hardness) and thus meets the production efficiency requirement of automobile dies. The rate of defects is well controlled. Compared with traditional technology, this technology has remarkable advantages and advancement.

A Ni25—10% SiC—X% graphene (mass fraction X = 0, 0.5, 1.0, 1.5) composite cladding layer was prepared on a 6063 aluminum alloy substrate using laser cladding in order to enhance the comprehensive performance of the aircraft refueling interface. The effect of the graphene content on the organization and properties of nickel-based silicon carbide composite cladding layers was investigated by laser melting. The macroscopic morphology, microstructure, hardness, elemental changes, corrosion and wear resistance of the cladding layer were studied by optical microscopy, scanning electron microscopy, a hardness tester, an X-ray diffractometer, an electrochemical workstation and an M-2000 frictional wear tester. The results indicated that the nickel-based clad layer without graphene incorporation had the worst forming, with a large number of pores and cracks in the cladding layer. Because graphene agglomerated easily, cracks were regenerated when the content of graphene was higher than 0.5%. The material phases of the cladding layer without graphene incorporation were mainly: Al3Ni2, Fe3Si and SiC. Due to the addition of graphene, the clad layer of specimen 2 was refined and a large number of hard phases, such as CrC and Cr23C6, were generated, which led to the increase in the hardness of the clad layer. When the content of graphene was further increased, the number of hard phases such as CrC and Cr23C6 produced in the cladding was relatively reduced due to the agglomeration of graphene, and the hardness of the cladding was reduced. As the impermeability of graphene reduces the diffusion of corrosive media to the substrate, the generation of hard-phase Al3Ni2 in the cladding layer makes the corrosion resistance of the cladding layer increase with the increase in graphene mass fraction. The result is that, when the content of graphene was 0.5%, the overall performance of the clad layer was the best, where its average hardness was increased by 40%, the average coefficient of friction was reduced by 12.7% and the wear rate was reduced by 60%.

The chaperonin-containing t-complex polypeptide 1 (CCT) is a molecular chaperone protein that is widely present in eukaryotic cytoplasm and can assist in the folding of newly synthesized proteins. The CCT complex consists of eight completely different subunits, among which the δ subunit plays an extremely important role in the folding and assembly of cytoskeleton proteins as an individual or complex with other subunits. In this study, we identified the CCTδ in the microsporidian Nosema bombycis (NbCCTδ) for the first time. The NbCCTδ gene contains a complete ORF of 1497 bp in length that encodes a 498 amino acid polypeptide. NbCCTδ is expressed throughout the entire lifecycle of N. bombycis and rather higher in early stage of proliferation. Indirect immunofluorescence results showed that NbCCTδ was colocalized with actin and β-tubulin during the proliferative and sporogonic phases of N. bombycis. RNA interference down-regulated the expression of the NbCCTδ gene. These results imply that NbCCTδ may participate in cytoskeleton formation and proliferation of N. bombycis.

To compare the influence of three operative approaches [transurethral en bloc resection of bladder tumor by pin-shaped electrode (pin-ERBT), transurethral resection of bladder tumor (TURBT) and transurethral holmium laser resection of bladder tumor (HoLRBT)] on the recurrence rate of non-muscle-invasive bladder cancer (NMIBC) at low dimension (i.e. diameter below 3 cm).A retrospective analysis was conducted for a total of 115 patients affected by solitary NMIBC, with a diameter3 cm, who were submitted to operation between March 2013 to May 2017. The patients were divided according to the operative method applied (pin-ERBT, TURBT and HoLRBT groups, respectively). The 2-year recurrence rate was compared among the three groups, and multivariat Cox hazard model analysis was applied to analyze the influencing factor(s) for postoperative recurrence.The 2-year recurrence rate was 10.0% in ERBT, 38.5% in TURBT and 40.0% in HoLRBT group, with a significant difference (P =0.014). According to the Cox hazard model analysis, age(HR=1.058, 95% CI: 1.019~1.098，P=0.003), operative method(HR=2.974,6.508, 95% CI: 0.862~10.255,1.657~25.566, P=0.023), smoking(HR=2.399, 95% CI: 1.147~5.017, P=0.020) and pathological grade(HR=2.012,95% CI: 1.279~3.165, P=0.002) were risk factors for postoperative recurrence of bladder cancer.Pin-ERBT can prominently decrease the postoperative recurrence rate of solitary NMIBC with a diameter3 cm.

Due to its high query speed and low storage cost, binary hashing has been widely used in approximate nearest neighbors (ANN) search. However, the binary bits are generally considered to be equal, which causes data points with different codes to share the same Hamming distance to the query sample. To solve the above distance measure ambiguity, bitwise weights methods were proposed. Unfortunately, in most of the existing methods, the bitwise weights and the binary codes are learnt separately in two stages, and their performances cannot be further improved. In this paper, to effectively address the above issues, we propose an adaptive mechanism that jointly generate the bitwise weights and the binary codes by preserving different types of similarity relationship. As a result, the binary codes are utilized to obtain the initial retrieval results, and they are further re-ranked by the weighted Hamming distance. This ANN search mechanism is termed AR-Rank in this paper. First, this joint mechanism allows the bitwise weights and the binary codes to be used as mutual feedback during the training stage, and they are well adapted to one other when the algorithm converges. Furthermore, the bitwise weights are required to preserve the relative similarity which is consistent with the nature of ANN search task. Thus, the data points can be accurately re-sorted based on the weighted Hamming distances. Evaluations on three datasets demonstrate that the proposed AR-Rank retrieval system outperforms nine state-of-the-art methods.

cir-ITCH, a well-known tumor-suppressive circular RNA, plays a critical role in different cancers. However, its expression and functional role in prostate cancer (PCa) are unclear. Herein, we explored the potential mechanism and tumor-inhibiting role of cir-ITCH in PCa. Using reverse transcriptase polymerase chain reaction assay, we analyzed the expression of cir-ITCH in PCa and paired adjacent nontumor tissue samples resected during surgical operation, as well as in two cell lines of human PCa (LNCaP and PC-3) and the immortalized normal prostate epithelial cell line (RWPE-1). Cell viability and migration of PCa cell lines were evaluated using CCK-8 and wound-healing assays. Expression of key proteins of the Wnt/β-catenin and PI3K/AKT/mTOR pathways was detected using western blotting. We found that cir-ITCH expression was typically downregulated in the tissues and cell lines of PCa compared to that in the peritumoral tissue and in RWPE-1 cells, respectively. The results showed that cir-ITCH overexpression significantly inhibits the proliferation, migration, and invasion of human PCa cells and that reciprocal inhibition of expression occurred between cir-ITCH and miR-17. Proteins in the Wnt/β-catenin and PI3K/AKT/mTOR pathways were downregulated by overexpression of cir-ITCH both in androgen receptor-positive LNCaP cells and androgen receptor-negative PC-3 cells. Taken together, these data demonstrated that cir-ITCH plays a tumor-suppressive role in human PCa cells, partly through the Wnt/β-catenin and PI3K/AKT/mTOR pathways. Thus, cir-ITCH may serve as a novel therapeutic target for the treatment of PCa, especially castration-resistant prostate cancer.

Aiming to solve the problem of goods popularity bias, this paper introduces the prevalence of items into user interest modeling, and proposes an item popularity model based on user interest feature. Usually, traditional model that does not take into account the stability of user’s interests, which leads to the difficulty in capturing their interest. To cope with this limitation, we propose a time-sensitive and stabilized interest similarity model that involves a process of calculating the similarity of user interest. Moreover, by combining those two kinds of similarity model based on weight factors, we develop a novel algorithm for calculation, which is named as IPSTS (IPSTS). To evaluate the proposed approach, experiments are performed and results indicate that Mean Absolute Difference (MAE) and root mean square error (RMSE) could be significantly reduced, when compared with those of traditional collaborative filtering Algorithms.

Aims This study aimed to identify suitable datasets for reanalysis and then explore potential key genes and related pathways of interstitial cystitis (IC). Methods We searched the Gene Expression Omnibus database and three expression profile datasets and included 23 lesions of IC and 9 normal tissues in the analysis. Eight urine specimens of patients with IC and five urine specimens of healthy controls were also included. Then, these datasets were reanalyzed to determine the differentially expressed genes (DEGs), which were used to perform Gene Ontology and pathway enrichment analyses. These identified candidate genes were also applied to generate a protein-protein interaction (PPI) network. Results Forty-two common DEGs were sorted and identified from two datasets, both of which included the samples of bladder lesions. Based on their functions and signaling pathways, these 42 DEGs are mainly classified as cell-surface proteins and are involved in the immune and inflammatory responses. The PPI network included 41 nodes. In this network, we identified 11 genes as central nodes that are involved in the immune system and the inflammatory response. Furthermore, IC with Hunner's lesions shared the same DEGs with IC without Hunner's lesions. In both subgroups (IC with and without Hunner's lesions), we identified some common DEGs shared between bladder lesions and urine samples. Conclusion Using bioinformatics, we integrated different IC-related datasets and identified potential critical genes involved in IC that may contribute to future research on IC.

This paper designs four kinds of three-dimensional negative Poisson’s ratio lattice structures, based on the two-dimensional concave hexagonal negative Poisson’s ratio honeycomb structure. Different parameters are designed for simulation analysis and experimental research, and the influence of different parameters on Poisson’s ratio is studied respectively. The main contents are as follows: Firstly, the three-dimensional solid model of lattice structure is established by modeling software, and the different structural parameters are determined, and the equivalent mechanical model is established. And according to the relevant structural parameters to establish the test scheme. Then, the finite element software is used to simulate the test core material with different parameters. According to the simulation results, the influence of different parameters on Poisson’s ratio is found out. Then the crashworthiness of the test board with different parameters is simulated and analyzed. According to the results, the most suitable structure is selected and applied to the engine hood. Finally, the simulation experiment of collision between pedestrian head and engine hood is carried out. The results of this paper compare the performance of lattice sandwich panels with different parameter structures in different tests and select the optimal combination of structural parameters. The adjustment and optimization direction of the structure is put forward according to the test results.

According to the pedestrian leg protection regulations, a collision with the simplified finite element model of the leg was carried out on the basis of incorporating EPP foam materials with different foaming rates and cushioning into the simplified front-end structure of the car. The results show that the front-end foam energy-absorbing structure of the vehicle cannot meet the pedestrian leg protection regulations. This paper designs a thin-walled steel structure and applies it to the front-end structure of the vehicle. After collision simulation with a simplified finite element model of the leg, the pedestrian’s tibial acceleration, knee joint shear displacement and knee joint bending angle are compared and analyzed. Pedestrian leg injury parameters, the results show that thin-walled steel structure is more suitable for giving full play to the energy absorption effect in an environment with insufficient energy absorption space than foam energy absorption structure.

Splicing factors are important components of RNA editing in eukaryotic organisms and can produce many functional and coding genes, which is an indispensable step for the correct expression of corresponding proteins. In this study, we identified splicing factor arginine/serine-rich 10 protein in the microsporidian Nosema bombycis and named it NbSRSF10. The NbSRSF10 gene contains a complete ORF of 1449 bp in length that encodes a 482-amino acid polypeptide. The isoelectric point (pI) of the protein encoded by NbSRSF10 gene was 4.94. NbSRSF10 has a molecular weight of 54.6 kD and has no signal peptide. NbSRSF10 is comprised of arginine (11.41%), glutamic acid (11.41%) and serine (9.54%) among the total amino acids, and 7 α-helix, 7 β-sheet and 15 random coils in secondary structure, and contains 71 phosphorylation sites, 22 N-glycosylation sites and 20 O-glycosylation sites. The three-dimensional structure of NbSRSF10 is similar to that of transformer-2 beta of Homo sapiens (hTra2-β). Indirect immunofluorescence showed that the NbSRSF10 is localized in the cytoplasm of the dormant microsporidian spore and is transferred to the nuclei when N. bombycis develops into the proliferative and sporogonic phase. qPCR revealed that the relative expression of NbSRSF10 increased in the meronts stage and was found at a relatively low level in the sporogonic phase of development of N. bombycis, and was up-regulated again during infection in the host cell and early proliferative phase of second life cycle. These results suggested that the NbSRSF10 may participate in the whole life cycle and play an important role in transcription regulation of N. bombycis.