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Keywords: Molecular rotor; Viscosity sensor; Temperature dependency EN Molecular rotor Viscosity sensor Temperature dependency 91 94 4 01/29/21 20210101 NES 210101 GLO:1CJF/01jan21:bkcs12110-toc-0001.jpg PHOTO (COLOR): . gl Different functions of molecular rotors based on viscosity sensitivity and temperature dependence. However, there are only a few temperature-dependency studies on BODIPY molecular rotors so far.11,13,14 Furthermore, existing studies cannot explain the relationship between temperature dependence and viscosity responses of molecular rotors. (i) The plots of viscosity sensitivity and temperature dependency. gl The presence of methyl groups on the BODIPY core usually inhibits the viscosity response of BODIPY rotors. Therefore, molecular rotors with excellent viscosity sensitivity may be more suitable as viscosity sensors, while rotors with moderate viscosity sensitivity are probably better as thermometers. [Extracted from the article]

Five new nonsymmetric BODIPY dyes based on the Biellmann methodology [NSBBs (nonsymmetrical Biellmann BODIPYs)] were prepared by condensation of pairs of different pyrroles with thiophosgene. Treatment of the resulting thioketones with MeI and BF3 ·Et2O/TEA yielded a wide range of products in a simple manner, with selective alkylation of each pyrrole of the indacene core and with controlled functionalization at the meso position. A comprehensive combined photophysical and computational study has been conducted to determine the impact of the alkylation pattern, as well as the role of the conformational freedom and electron coupling of 8-phenyl and 8-methylthio groups on the spectroscopic signatures of BODIPYs. The results and conclusions reached are compared with those for previously reported related analogues. We intend to provide key structural guidelines with regard to the factors that trigger the fluorescence responses and spectral shifts of multifunctional fluorophores of this family. [ABSTRACT FROM AUTHOR]

Aim: Sporothrix schenckii is the causative agent of sporotrichosis. A 70-kDa glycoprotein, Gp70, is a candidate for the development of prophylactic alternatives to control the disease, and its gene (GP70) is predicted to encode for a protein of 43 kDa, contrasting with the molecular weight of the native protein., Materials & Methods: The GP70 was expressed in bacteria, the recombinant protein purified, used in immunoassays and injected to Galleria mellonella., Results & Conclusion: The recombinant protein was detected by anti-Gp70 antibodies, confirming that the Gp70 backbone is a 43-kDa peptide. This protein showed enzyme activity of cyclase and was recognized by sera of patients with sporotrichosis. Although it was not useful for serodiagnosis of sporotrichosis, it conferred protection to animals against experimental sporotrichosis.