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The human activities change the landscape and remote sensing thats generates data that allow us to analyze such changes. In this sense, the present study aimed to evaluate the use and occupation of land in the municipality Marapanim based on TerraClass data for the years 2008 and 2010. For this, was used the data bank TerraClass Project and the municipal limits established by the Brazilian Institute of Geography and Statistics, which with the use of SPRING software, Version 5.2.4, which was drawn up thematic map of the city with respect to changed and unchanged areas and there was the cross-tabulation of the data. It can be seen that the region of freshwater Marapanim, the municipality presented major changes, especially in mosaics classes of occupations, and secondary vegetation. These changes may result from the strong presence of shifting cultivators in place, the other classes studied did not change significantly. The large number of observed areas may have influenced the results, so, it is necessary a field study with the local communities in the municipality. [ABSTRACT FROM AUTHOR]

This study aimed at assessing the effect of different concentrations of the growth factor similar to insulin 1 (IGF-1) in the development, survival and ultrastructure of the bovine preantral follicles cultured in situ. Fragments of bovine ovarian cortical tissue were cultured during 1 and 7 days in 1 ml of α-MEM(+) , supplemented with different concentrations of human recombinant IGF-1 (0, 30, 70 and 100 ng/ml), in an incubator at 37°C and 5% of CO2 in 24-well plates with total replacement of the medium every 2 days. Non-cultured ovarian fragments (control) and ovarian fragments cultured during 1 and 7 days were processed for classic histology, mechanical isolation and electron transmission microscopy (ETM). Parameters such as normality, viability, activation, development, diameter and ultrastructure were evaluated. All statistical analyses were carried out using sas Version 9.2. The results showed that the percentage of follicles morphologically normal in the IGF-1 30 ng/ml treatment was similar to the fresh control (p > 0.05) both on the day 1 and on the day 7 of in vitro culture. In the viability analysis, the cultured treatments maintained the percentage of viable follicles during the entire culture period (p > 0.05). After 7 days of culture, the IGF-1 30 ng/ml treatment showed higher percentages of developing follicles (48.33%) than those of the fresh control (22.22%) and the cultured treatments (p < 0.05). Also, after 7 days of culture, IGF-1 30 ng/ml presented a higher follicular diameter when compared to the control and other concentrations of IGF-1 tested. Ultrastructurally, the non-cultured control and IGF-1 30 ng/ml, after 7 days of culture, showed conserved oocytes, nuclei and organelles. Hence, it is concluded that IGF-1 30 ng/ml was the most efficient concentration for the development of bovine preantral follicles cultured in vitro., (© 2016 Blackwell Verlag GmbH.)