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Your search keyword '"GOTO-KAZETO, RIE"' showing total 17 results
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17 results on '"GOTO-KAZETO, RIE"'

8. Visualization and motility of primordial germ cells using green fluorescent protein fused to 3'UTR of common carp nanos-related gene

Author

Kawakami, Yutaka, Saito, Taiju, Fujimoto, Takafumi, Goto-Kazeto, Rie, Takahashi, Eisuke, Adachi, Shinji, Arai, Katsutoshi, and Yamaha, Etsuro

Subjects
germline chimera, primordial germ cell, common carp, goldfish, nanos
Abstract

Primordial germ cells (PGCs) are the only cells in developing embryos with the potential to transmit genetic information to the next generation. We previously visualized the PGCs of several teleostean embryos by injecting RNA synthesized from constructs encoding green fluorescent protein (GFP) fused to the 3'UTR of the zebrafish (Danio rerio) nanos1 gene (nos1). However, this technique was not always suitable for visualizing PGCs in embryos from all teleost species. In this study, we compared the visualization of PGCs in common carp (Cyprinus carpio) embryos using two artificial constructs containing GFP fused to the 3'UTR of nanos from either common carp or zebrafish. Visualization was better using GFP fused to the 3'UTR of the nanos gene from common carp, compared with that from zebrafish. The visualized PGCs successfully migrated toward the gonadal ridge after transplantation into goldfish host embryos, suggesting that they maintained normal migratory motility. These techniques could be useful for the production of inter-specific germline chimeras using common carp donor PGCs.

Published
2011

9. Developmental biotechnology for aquaculture, with special reference to surrogate production in teleost fishes

Author

1000060191376, Yamaha, Etsuro, Saito, Taiju, Goto-Kazeto, Rie, 1000000137902, Arai, Katsutoshi, 1000060191376, Yamaha, Etsuro, Saito, Taiju, Goto-Kazeto, Rie, 1000000137902, and Arai, Katsutoshi

Abstract

This review introduces surrogate production as a new technique for fish-seed production in aquaculture. Surrogate production in fish is a technique used to obtain the gametes of a certain genotype through the gonad of another genotype. It is achieved by inducing germ-line chimerism between different species during early development. Primordial germ cells (PGCs) are the key material of this technique to induce germ-line chimera. In several species, it has been reported that PGCs differentiated from the blastomeres inherited some maternally supplied mRNA located in the terminal regions of the early cleavage furrows. PGCs from donor species (or strains) are isolated and transplanted into host species to induce the germ-line chimera. Four methods for inducing germ-line chimera are described: blastomere transplantation, blastoderm-graft transplantation, transplantation of PGC from the genital ridge, and transplantation visualised PGC with GFP fluorescence. Several problems preventing the successful induction of germ-line chimera in various fish species are discussed. Surrogate production, however, opens the possibility of efficient fish-seed production and effective breeding and transfer of biodiversity to an aquaculture strain. Conservation and efficient utilisation of genetic resources will be achieved through surrogate production combined with the cryopreservation of PGCs.

Published
2007

16. Molecular characterization of three forms of putative membrane-bound progestin receptors and their tissue-distribution in channel catfish, Ictalurus punctatus

Author

Kazeto, Yukinori, Goto-Kazeto, Rie, Thomas, Peter, and Trant, John M

Abstract

Membrane-bound progestin receptors (mPR) were recently cloned and characterized as a new class of steroid receptors that transduce cell-signals through alteration of MAP kinase- and cAMP-dependent pathways. To further develop our understanding of this new class of steroid receptors, we characterized the cDNAs and genes of theα, β and γ forms of the channel catfish mPRs (IpmPR). The predicted α and β proteins have 49% sequence identity, whereas they only have 30% and 27% identities, respectively, with the γ form. Furthermore, IpmPRα and IpmPRβ genes have similar structures featuring intronless coding regions, while IpmPRγ gene is composed of 8 exons and 7 introns. The 5′-flanking region of each IpmPR gene differs, but each contains putative transcriptional regulatory elements of factors known to influence reproductive physiology and endocrine disruption, for example, responsive elements for cAMP and steroids and the recognition sites for steroidogenic factor-1 and for the aryl hydrocarbon receptor. The IpmPRα gene was detected in all the tissues tested with relatively greater expression in brain, pituitary, muscle and testis. The expression of IpmPRβ was much lower than that of IpmPRα and the transcript was predominantly observed in brain, pituitary, ovary and testis. In contrast, the IpmPRγ transcript was mainly detected in gill, ventral aorta, intestine, and trunk kidney. In conclusion, all the structural features of the IpmPRs strongly suggest that the closely related α and β forms are distantly related to the γ form. Additionally, regulatory features of the 5′-flanking regions and the differences in tissue-specific expression of each IpmPR gene suggest that they are involved in different endocrine functions in catfish.

Published
2005
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