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36 results on '"GREEN, SABRINA"'

1. Personalized bacteriophage therapy outcomes for 100 consecutive cases: a multicentre, multinational, retrospective observational study

Author

Pirnay, Jean-Paul, Djebara, Sarah, Steurs, Griet, Griselain, Johann, Cochez, Christel, De Soir, Steven, Glonti, Tea, Spiessens, An, Vanden Berghe, Emily, Green, Sabrina, Wagemans, Jeroen, Lood, Cédric, Schrevens, Eddie, Chanishvili, Nina, Kutateladze, Mzia, de Jode, Mathieu, Ceyssens, Pieter-Jan, Draye, Jean-Pierre, Verbeken, Gilbert, De Vos, Daniel, Rose, Thomas, Onsea, Jolien, Van Nieuwenhuyse, Brieuc, Soentjens, Patrick, Lavigne, Rob, and Merabishvili, Maya

Published
2024
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2. A Retrospective, Observational Study of 12 Cases of Expanded-Access Customized Phage Therapy: Production, Characteristics, and Clinical Outcomes.

Author

Green, Sabrina, Clark, Justin, Santos, Haroldo, Weesner, Kyle, Salazar, Keiko, Aslam, Saima, Campbell, J, Blodget, Emily, Morris, Michele, Suh, Gina, Obeid, Karam, Silveira, Fernanda, Filippov, Andrey, Whiteson, Katrine, Trautner, Barbara, Terwilliger, Austen, Maresso, Anthony, and Doernberg, Sarah

Subjects
antibiotic resistance, microbiology, phage, phage therapy, Humans, Anti-Bacterial Agents, Bacteria, Bacterial Infections, Bacteriophages, Phage Therapy, Retrospective Studies
Abstract

BACKGROUND: Antimicrobial resistance (AMR) is undermining modern medicine, a problem compounded by bacterial adaptation to antibiotic pressures. Phages are viruses that infect bacteria. Their diversity and evolvability offer the prospect of their use as a therapeutic solution. Reported are outcomes of customized phage therapy for patients with difficult-to-treat antimicrobial resistant infections. METHODS: We retrospectively assessed 12 cases of customized phage therapy from a phage production center. Phages were screened, purified, sequenced, characterized, and Food and Drug Administration-approved via the IND (investigational new drug) compassionate-care route. Outcomes were assessed as favorable or unfavorable by microbiologic and clinical standards. Infections were device-related or systemic. Other experiences such as time to treatment, antibiotic synergy, and immune responses were recorded. RESULTS: Fifty requests for phage therapy were received. Customized phages were generated for 12 patients. After treatment, 42% (5/12) of cases showed bacterial eradication and 58% (7/12) showed clinical improvement, with two-thirds of all cases (66%) showing favorable responses. No major adverse reactions were observed. Antibiotic-phage synergy in vitro was observed in most cases. Immunological neutralization of phages was reported in 5 cases. Several cases were complicated by secondary infections. Complete characterization of the phages (morphology, genomics, and activity) and their production (methods, sterility, and endotoxin tests) are reported. CONCLUSIONS: Customized phage production and therapy was safe and yielded favorable clinical or microbiological outcomes in two-thirds of cases. A center or pipeline dedicated to tailoring the phages against a patients specific AMR bacterial infection may be a viable option where standard treatment has failed.

Published
2023

3. Phage Therapy Related Microbial Succession Associated with Successful Clinical Outcome for a Recurrent Urinary Tract Infection

Author

Terwilliger, Austen, Clark, Justin, Karris, Maile, Hernandez-Santos, Haroldo, Green, Sabrina, Aslam, Saima, and Maresso, Anthony

Subjects
Microbiology, Biological Sciences, Infectious Diseases, Emerging Infectious Diseases, Urologic Diseases, Biotechnology, 5.1 Pharmaceuticals, Development of treatments and therapeutic interventions, Infection, Anti-Bacterial Agents, Bacteriophages, Escherichia coli, Escherichia coli Infections, Humans, Liver Transplantation, Male, Microbial Sensitivity Tests, Middle Aged, Phage Therapy, Precision Medicine, Transplant Recipients, Urinary Tract Infections, Virulence, phage therapy, microbial pathogenesis, UTI, humoral response, comparative genomics
Abstract

We rationally designed a bacteriophage cocktail to treat a 56-year-old male liver transplant patient with complex, recurrent prostate and urinary tract infections caused by an extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli (E. coli) (UCS1). We screened our library for phages that killed UCS1, with four promising candidates chosen for their virulence, mucolytic properties, and ability to reduce bacterial resistance. The patient received 2 weeks of intravenous phage cocktail with concomitant ertapenem for 6 weeks. Weekly serum and urine samples were collected to track the patient's response. The patient tolerated the phage therapy without any adverse events with symptom resolution. The neutralization of the phage activity occurred with sera collected 1 to 4 weeks after the first phage treatment. This was consistent with immunoassays that detected the upregulation of immune stimulatory analytes. The patient developed asymptomatic recurrent bacteriuria 6 and 11 weeks following the end of phage therapy-a condition that did not require antibiotic treatment. The bacteriuria was caused by a sister strain of E. coli (UCS1.1) that remained susceptible to the original phage cocktail and possessed putative mutations in the proteins involved in adhesion and invasion compared to UCS1. This study highlights the utility of rationally designed phage cocktails with antibiotics at controlling E. coli infection and suggests that microbial succession, without complete eradication, may produce desirable clinical outcomes.

Published
2021

4. Addressing the Research and Development Gaps in Modern Phage Therapy

Author

Turner, Paul E., primary, Azeredo, Joana, additional, Buurman, Ed T., additional, Green, Sabrina, additional, Haaber, Jakob Krause, additional, Haggstrom, Douglas, additional, Kameda de Figueiredo Carvalho, Koichi, additional, Kirchhelle, Claas, additional, Gonzalez Moreno, Mercedes, additional, Pirnay, Jean-Paul, additional, and Portillo, Mirza Alas, additional

Published
2024
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5. Use of the Naturally Occurring Bacteriophage Grouping Model for the Design of Potent Therapeutic Cocktails.

Author

Glonti, Tea, Goossens, Michael, Cochez, Christel, Green, Sabrina, Gorivale, Sayali, Wagemans, Jeroen, Lavigne, Rob, and Pirnay, Jean-Paul

Subjects
KLEBSIELLA pneumoniae, DRUG resistance in bacteria, BACTERIOPHAGES, BACTERIAL cultures, BACTERIAL diseases
Abstract

The specificity of phages and their ability to evolve and overcome bacterial resistance make them potentially useful as adjuncts in the treatment of antibiotic-resistant bacterial infections. The goal of this study was to mimic a natural grouping of phages of interest and to evaluate the nature of their proliferation dynamics with bacteria. We have, for the first time, transferred naturally occurring phage groups directly from their sources of isolation to in vitro and identified 13 P. aeruginosa and 11 K. pneumoniae phages of 18 different genera, whose host range was grouped as 1.2–17%, 28–48% and 60–87%, using a large collection of P. aeruginosa (n = 102) and K. pneumoniae (n = 155) strains carrying different virulence factors and phage binding receptors. We introduced the interpretation model curve for phage liquid culturing, which allows easy and quick analysis of bacterial and phage co-proliferation and growth of phage-resistant mutants (PRM) based on qualitative and partially quantitative evaluations. We assayed phage lytic activities both individually and in 14 different cocktails on planktonic bacterial cultures, including three resistotypes of P. aeruginosa (PAO1, PA14 and PA7) and seven K. pneumoniae strains of different capsular serotypes. Based on the results, the natural phage cocktails designed and tested in this study largely performed well and inhibited PRM growth either synergistically or in proto-cooperation. This study contributes to the knowledge of phage behavior in cocktails and the formulation of therapeutic phage preparations. The paper also provides a detailed description of the methods of working with phages. [ABSTRACT FROM AUTHOR]

Published
2024
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6. Understanding the bacterial imbalance in Hidradenitis Suppurativa patients: Insights into microbial community shifts and colonization by opportunistic pathogens

Author

Bens, Lene, primary, Vanhoutvin, Tine, additional, Kerremans, Alison, additional, Jansen, Daan, additional, Depypere, Melissa, additional, Hillary, Tom, additional, Vermeire, Severine, additional, Green, Sabrina I, additional, Matthijnssens, Jelle, additional, Sabino, Joao, additional, Lavigne, Rob, additional, Van Laethem, An, additional, and Wagemans, Jeroen, additional

Published
2023
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8. Phage therapy for hidradenitis suppurativa: a unique challenge and possible opportunity for personalized treatment of a complex, inflammatory disease

Author

Bens, Lene, primary, Green, Sabrina I, additional, Jansen, Daan, additional, Hillary, Tom, additional, Vanhoutvin, Tine, additional, Matthijnssens, Jelle, additional, Sabino, João, additional, Vermeire, Séverine, additional, Van Laethem, An, additional, Wagemans, Jeroen , additional, and Lavigne, Rob, additional

Published
2023
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11. Corrigendum: Development of phage cocktails to treat E. coli catheter-associated urinary tract infection and associated biofilms

Author

Sanchez, Belkys C., primary, Heckmann, Emmaline R., additional, Green, Sabrina I., additional, Clark, Justin R., additional, Kaplan, Heidi B., additional, Ramig, Robert F., additional, Muldrew, Kenneth L., additional, Hines-Munson, Casey, additional, Skelton, Felicia, additional, Trautner, Barbara W., additional, and Maresso, Anthony W., additional

Published
2022
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12. Development of Phage Cocktails to Treat E. coli Catheter-Associated Urinary Tract Infection and Associated Biofilms

Author

Sanchez, Belkys C., primary, Heckmann, Emmaline R., additional, Green, Sabrina I., additional, Clark, Justin R., additional, Kaplan, Heidi B., additional, Ramig, Robert F., additional, Muldrew, Kenneth L., additional, Hines-Munson, Casey, additional, Skelton, Felicia, additional, Trautner, Barbara W., additional, and Maresso, Anthony W., additional

Published
2022
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13. Identifying Causative Microorganisms in Left Ventricular Assist Device Infections as a Guide for Developing Bacteriophage Therapy

Author

Kamat, Ishan, primary, Lamba, Harveen, additional, Hines-Munson, Casey, additional, Hudson, Samuel, additional, Liao, Kenneth, additional, Muldrew, Kenneth L., additional, Green, Sabrina, additional, Terwilliger, Austen, additional, Kaplan, Heidi B., additional, Ramig, Robert F., additional, Maresso, Anthony, additional, and Trautner, Barbara W., additional

Published
2022
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14. Moral Solidarity, Relationships and Relational Egalitarianism

Author

Green, Sabrina

Abstract

In my thesis, I consider Jean Harvey's account of moral solidarity. Harvey claims that solidarity only occurs between people who share a personal relationship. I raise a concern regarding the limited scope of Harvey's account of moral solidarity. I challenge the view that one cannot extend the concerns they have for a friend experiencing injustice to a stranger who is also experiencing the same injustice. In order to address this concern and defend Harvey's account of solidarity, I offer a friendly amendment to her account. By drawing on Samuel Scheffler's account of relational egalitarianism, and in light of the relationships citizens may share with one another, I expand the types of relationships that should be considered solidarity under an account such as Harvey's My account thus defends a more expansive view of solidarity than that of Harvey.

Published
2022
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16. Targeting of Mammalian Glycans Enhances Phage Predation in the Gastrointestinal Tract

Author

Green, Sabrina I., primary, Gu Liu, Carmen, additional, Yu, Xue, additional, Gibson, Shelley, additional, Salmen, Wilhem, additional, Rajan, Anubama, additional, Carter, Hannah E., additional, Clark, Justin R., additional, Song, Xuezheng, additional, Ramig, Robert F., additional, Trautner, Barbara W., additional, Kaplan, Heidi B., additional, and Maresso, Anthony W., additional

Published
2021
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17. Enteroaggregative E. coli Adherence to Human Heparan Sulfate Proteoglycans Drives Segment and Host Specific Responses to Infection

Author

Rajan, Anubama, primary, Robertson, Matthew J., additional, Carter, Hannah E., additional, Poole, Nina M., additional, Clark, Justin R., additional, Green, Sabrina I., additional, Criss, Zachary K., additional, Zhao, Boyang, additional, Karandikar, Umesh, additional, Xing, Yikun, additional, Margalef-Català, Mar, additional, Jain, Nikhil, additional, Wilson, Reid L., additional, Bai, Fan, additional, Hyser, Joseph M., additional, Petrosino, Joseph, additional, Shroyer, Noah F., additional, Blutt, Sarah E., additional, Coarfa, Cristian, additional, Song, Xuezheng, additional, Prasad, BV Venkataram, additional, Amieva, Manuel R., additional, Grande-Allen, Jane, additional, Estes, Mary K., additional, Okhuysen, Pablo C., additional, and Maresso, Anthony W., additional

Published
2020
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20. Targeting of Mammalian Glycans Enhances Phage Predation in the Gastrointestinal Tract

Author

Green, Sabrina I., primary, Liu, Carmen Gu, additional, Yu, Xue, additional, Gibson, Shelley, additional, Salmen, Wilhem, additional, Rajan, Anubama, additional, Carter, Hannah E., additional, Clark, Justin R., additional, Song, Xuezheng, additional, Ramig, Robert F., additional, Trautner, Barbara W., additional, Kaplan, Heidi B., additional, and Maresso, Anthony W., additional

Published
2020
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21. Tailored Antibacterials and Innovative Laboratories for Phage (Φ) Research: Personalized Infectious Disease Medicine for the Most Vulnerable At-Risk Patients

Author

Terwilliger, Austen L., primary, Gu Liu, Carmen, additional, Green, Sabrina I., additional, Clark, Justin R., additional, Salazar, Keiko C., additional, Hernandez Santos, Haroldo, additional, Heckmann, Emmaline R., additional, Trautner, Barbara W., additional, Ramig, Robert F., additional, and Maresso, Anthony W., additional

Published
2020
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34. Evaluation of Gastric Lavage Method for Diet Analysis of the Eastern Red-backed Salamander (Plethodon cinereus).

Author

BONDI, CHERYL A., GREEN, SABRINA, and BEIER, COLIN M.

Subjects
*GASTRIC lavage, *PLETHODON cinereus, *DIET research, *DISSECTING microscopes, *GASTROINTESTINAL contents
Abstract

The article reports on evaluation of gastric lavage method for diet analysis of Plethodon cinereus. It states that animals were dissected using stereomicroscope to assess stomach contents while identified prey items were sent to taxonomic resolution possible. It mentions that stomach flushing is a method to collect diet samples removing most of the stomach contents and gastric lavage is an effective technique for studying plethodontid diets without removing individuals from population.

Published
2015

35. Moral Solidarity, Relationships and Relational Egalitarianism

Author

Green, Sabrina Ruby

Subjects
solidarity, egalitarianism, relationships, social philosophy
Abstract

In my thesis, I consider Jean Harvey’s account of moral solidarity. Harvey claims that solidarity only occurs between people who share a personal relationship. I raise a concern regarding the limited scope of Harvey’s account of moral solidarity. I challenge the view that one cannot extend the concerns they have for a friend experiencing injustice to a stranger who is also experiencing the same injustice. In order to address this concern and defend Harvey’s account of solidarity, I offer a friendly amendment to her account. By drawing on Samuel Scheffler’s account of relational egalitarianism, and in light of the relationships citizens may share with one another, I expand the types of relationships that should be considered solidarity under an account such as Harvey’s My account thus defends a more expansive view of solidarity than that of Harvey.

Published
2022

36. Role for FimH in Extraintestinal Pathogenic Escherichia coliInvasion and Translocation through the Intestinal Epithelium

Author

Poole, Nina M., Green, Sabrina I., Rajan, Anubama, Vela, Luz E., Zeng, Xi-Lei, Estes, Mary K., and Maresso, Anthony W.

Abstract

ABSTRACTThe translocation of bacteria across the intestinal epithelium of immunocompromised patients can lead to bacteremia and life-threatening sepsis. Extraintestinal pathogenic Escherichia coli(ExPEC), so named because this pathotype infects tissues distal to the intestinal tract, is a frequent cause of such infections, is often multidrug resistant, and chronically colonizes a sizable portion of the healthy population. Although several virulence factors and their roles in pathogenesis are well described for ExPEC strains that cause urinary tract infections and meningitis, they have not been linked to translocation through intestinal barriers, a fundamentally distant yet important clinical phenomenon. Using untransformed ex situhuman intestinal enteroids and transformed Caco-2 cells, we report that ExPEC strain CP9 binds to and invades the intestinal epithelium. ExPEC harboring a deletion of the gene encoding the mannose-binding type 1 pilus tip protein FimH demonstrated reduced binding and invasion compared to strains lacking known E. colivirulence factors. Furthermore, in a murine model of chemotherapy-induced translocation, ExPEC lacking fimHcolonized at levels comparable to that of the wild type but demonstrated a statistically significant reduction in translocation to the kidneys, spleen, and lungs. Collectively, this study indicates that FimH is important for ExPEC translocation, suggesting that the type 1 pilus is a therapeutic target for the prevention of this process. Our study also highlights the use of human intestinal enteroids in the study of enteric diseases.

Published
2017
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