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2. Risk and predictive factors of prolonged viral RNA shedding in upper respiratory specimens in a large cohort of COVID-19 patients admitted to an Italian reference hospital

Author

Abbonizio, Maria Alessandra, Abdeddaim, Amina, Agostini, Elisabetta, Agrati, Chiara, Albarello, Fabrizio, Amadei, Gioia, Amendola, Alessandra, Antinori, Andrea, Antonica, Maria Assunta, Antonini, Mario, Bartoli, Tommaso Ascoli, Baldini, Francesco, Barbaro, Raffaella, Bartolini, Barbara, Bellagamba, Rita, Benigni, Martina, Bevilacqua, Nazario, Biava, Gianluigi, Bibas, Michele, Bordi, Licia, Bordoni, Veronica, Boumis, Evangelo, Branca, Marta, Buonomo, Rosanna, Busso, Donatella, Camici, Marta, Campioni, Paolo, Canichella, Flaminia, Capobianchi, Maria Rosaria, Capone, Alessandro, Caporale, Cinzia, Caraffa, Emanuela, Caravella, Ilaria, Carletti, Fabrizio, Castilletti, Concetta, Cataldo, Adriana, Cerilli, Stefano, Cerva, Carlotta, Chiappini, Roberta, Chinello, Pierangelo, Cianfarani, Maria Assunta, Ciaralli, Carmine, Cimaglia, Claudia, Cinicola, Nicola, Ciotti, Veronica, Cicalini, Stefania, Colavita, Francesca, Corpolongo, Angela, Cristofaro, Massimo, Curiale, Salvatore, D’Abramo, Alessandra, Dantimi, Cristina, De Angelis, Alessia, De Angelis, Giada, De Palo, Maria Grazia, De Zottis, Federico, Di Bari, Virginia, Di Lorenzo, Rachele, Di Stefano, Federica, D’Offizi, Gianpiero, Donno, Davide, Evangelista, Francesca, Faraglia, Francesca, Farina, Anna, Ferraro, Federica, Fiorentini, Lorena, Frustaci, Andrea, Fusetti, Matteo, Galati, Vincenzo, Gagliardini, Roberta, Gallì, Paola, Garotto, Gabriele, Gaviano, Ilaria, Tekle, Saba Gebremeskel, Giancola, Maria Letizia, Giansante, Filippo, Giombini, Emanuela, Granata, Guido, Greci, Maria Cristina, Grilli, Elisabetta, Grisetti, Susanna, Gualano, Gina, Iacomi, Fabio, Iaconi, Marta, Iannicelli, Giuseppina, Inversi, Carlo, Ippolito, Giuseppe, Lalle, Eleonora, Lamanna, Maria Elena, Lanini, Simone, Lapa, Daniele, Lepore, Luciana, Libertone, Raffaella, Lionetti, Raffaella, Liuzzi, Giuseppina, Loiacono, Laura, Lucia, Andrea, Lufrani, Franco, Macchione, Manuela, Maffongelli, Gaetano, Marani, Alessandra, Marchioni, Luisa, Mariano, Andrea, Marini, Maria Cristina, Maritti, Micaela, Mastrobattista, Annelisa, Mastrorosa, Ilaria, Matusali, Giulia, Mazzotta, Valentina, Mencarini, Paola, Meschi, Silvia, Messina, Francesco, Micarelli, Sibiana, Mogavero, Giulia, Mondi, Annalisa, Montalbano, Marzia, Montaldo, Chiara, Mosti, Silvia, Murachelli, Silvia, Musso, Maria, Nardi, Michela, Navarra, Assunta, Nicastri, Emanuele, Nocioni, Martina, Noto, Pasquale, Noto, Roberto, Oliva, Alessandra, Onnis, Ilaria, Ottou, Sandrine, Palazzolo, Claudia, Pallini, Emanuele, Palmieri, Fabrizio, Palombi, Giulio, Pareo, Carlo, Passeri, Virgilio, Pelliccioni, Federico, Penna, Giovanna, Petrecchia, Antonella, Petrone, Ada, Petrosillo, Nicola, Pianura, Elisa, Pinnetti, Carmela, Pisciotta, Maria, Piselli, Pierluca, Pittalis, Silvia, Pontarelli, Agostina, Proietti, Costanza, Puro, Vincenzo, Ramazzini, Paolo Migliorisi, Rianda, Alessia, Rinonapoli, Gabriele, Rosati, Silvia, Rubino, Dorotea, Rueca, Martina, Ruggeri, Alberto, Sacchi, Alessandra, Sampaolesi, Alessandro, Sanasi, Francesco, Santagata, Carmen, Scarabello, Alessandra, Scarcia, Silvana, Schininà, Vincenzo, Scognamiglio, Paola, Scorzolini, Laura, Stazi, Giulia, Strano, Giacomo, Taglietti, Fabrizio, Taibi, Chiara, Taloni, Giorgia, Nardi, Tetaj, Tonnarini, Roberto, Topino, Simone, Tozzi, Martina, Vaia, Francesco, Vairo, Francesco, Valli, Maria Beatrice, Vergori, Alessandra, Vincenzi, Laura, Visco-Comandini, Ubaldo, Vita, Serena, Vittozzi, Pietro, Zaccarelli, Mauro, Zanetti, Antonella, Zito, Sara, Lorenzini, Patrizia, Di Gennaro, Francesco, and Girardi, Enrico

Published
2021
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5. Rhodococcus equi infection in HIV-infected individuals: case reports and review of the literature

Author

Topino, Simone, Galati, Vincenzo, Grilli, Elisabetta, and Petrosillo, Nicola

Subjects
Gram-positive bacterial infections -- Risk factors, Gram-positive bacterial infections -- Research, HIV patients -- Health aspects, Health
Abstract

Rhodococcus equi is a gram-positive, coryneform bacterium that causes zoonotic infection mainly in horses and foals. It sometimes affects humans presenting as cavitary pneumonia. Immunocompromised patients, including HIV-infected patients, are more susceptible to R. equi infection. We present 10 cases of R. equi infection in HIV-positive patients admitted to our institute from 1991 to June 2008. Moreover, we have reviewed 272 cases of R. equi infection in HIV-infected persons, published from 1986 through 2008. With respect to the literature data, the R. equi strains isolated in our case series showed lower sensitivity to ceftriaxone, amoxicillin/clavulanic acid, and cotrimoxazole. Prompt diagnosis, early initiation of antiretroviral treatment and combined antimicrobial treatment seem to be effective to eradicate the infection and to improve the outcome. DOI: 10.1089/apc.2009.0248

Published
2010

6. Risk and predictive factors of prolonged viral RNA shedding in upper respiratory specimens in a large cohort of COVID-19 patients admitted to an Italian reference hospital

Author

Mondi, Annalisa, primary, Lorenzini, Patrizia, additional, Castilletti, Concetta, additional, Gagliardini, Roberta, additional, Lalle, Eleonora, additional, Corpolongo, Angela, additional, Valli, Maria Beatrice, additional, Taglietti, Fabrizio, additional, Cicalini, Stefania, additional, Loiacono, Laura, additional, Di Gennaro, Francesco, additional, D’Offizi, Gianpiero, additional, Palmieri, Fabrizio, additional, Nicastri, Emanuele, additional, Agrati, Chiara, additional, Petrosillo, Nicola, additional, Ippolito, Giuseppe, additional, Vaia, Francesco, additional, Girardi, Enrico, additional, Capobianchi, Maria Rosaria, additional, Antinori, Andrea, additional, Zito, Sara, additional, Abbonizio, Maria Alessandra, additional, Abdeddaim, Amina, additional, Agostini, Elisabetta, additional, Albarello, Fabrizio, additional, Amadei, Gioia, additional, Amendola, Alessandra, additional, Antonica, Maria Assunta, additional, Antonini, Mario, additional, Bartoli, Tommaso Ascoli, additional, Baldini, Francesco, additional, Barbaro, Raffaella, additional, Bartolini, Barbara, additional, Bellagamba, Rita, additional, Benigni, Martina, additional, Bevilacqua, Nazario, additional, Biava, Gianluigi, additional, Bibas, Michele, additional, Bordi, Licia, additional, Bordoni, Veronica, additional, Boumis, Evangelo, additional, Branca, Marta, additional, Buonomo, Rosanna, additional, Busso, Donatella, additional, Camici, Marta, additional, Campioni, Paolo, additional, Canichella, Flaminia, additional, Capone, Alessandro, additional, Caporale, Cinzia, additional, Caraffa, Emanuela, additional, Caravella, Ilaria, additional, Carletti, Fabrizio, additional, Cataldo, Adriana, additional, Cerilli, Stefano, additional, Cerva, Carlotta, additional, Chiappini, Roberta, additional, Chinello, Pierangelo, additional, Cianfarani, Maria Assunta, additional, Ciaralli, Carmine, additional, Cimaglia, Claudia, additional, Cinicola, Nicola, additional, Ciotti, Veronica, additional, Colavita, Francesca, additional, Cristofaro, Massimo, additional, Curiale, Salvatore, additional, D’Abramo, Alessandra, additional, Dantimi, Cristina, additional, De Angelis, Alessia, additional, De Angelis, Giada, additional, De Palo, Maria Grazia, additional, De Zottis, Federico, additional, Di Bari, Virginia, additional, Di Lorenzo, Rachele, additional, Di Stefano, Federica, additional, Donno, Davide, additional, Evangelista, Francesca, additional, Faraglia, Francesca, additional, Farina, Anna, additional, Ferraro, Federica, additional, Fiorentini, Lorena, additional, Frustaci, Andrea, additional, Fusetti, Matteo, additional, Galati, Vincenzo, additional, Gallì, Paola, additional, Garotto, Gabriele, additional, Gaviano, Ilaria, additional, Tekle, Saba Gebremeskel, additional, Giancola, Maria Letizia, additional, Giansante, Filippo, additional, Giombini, Emanuela, additional, Granata, Guido, additional, Greci, Maria Cristina, additional, Grilli, Elisabetta, additional, Grisetti, Susanna, additional, Gualano, Gina, additional, Iacomi, Fabio, additional, Iaconi, Marta, additional, Iannicelli, Giuseppina, additional, Inversi, Carlo, additional, Lamanna, Maria Elena, additional, Lanini, Simone, additional, Lapa, Daniele, additional, Lepore, Luciana, additional, Libertone, Raffaella, additional, Lionetti, Raffaella, additional, Liuzzi, Giuseppina, additional, Lucia, Andrea, additional, Lufrani, Franco, additional, Macchione, Manuela, additional, Maffongelli, Gaetano, additional, Marani, Alessandra, additional, Marchioni, Luisa, additional, Mariano, Andrea, additional, Marini, Maria Cristina, additional, Maritti, Micaela, additional, Mastrobattista, Annelisa, additional, Mastrorosa, Ilaria, additional, Matusali, Giulia, additional, Mazzotta, Valentina, additional, Mencarini, Paola, additional, Meschi, Silvia, additional, Messina, Francesco, additional, Micarelli, Sibiana, additional, Mogavero, Giulia, additional, Mondi, Annalisa, additional, Montalbano, Marzia, additional, Montaldo, Chiara, additional, Mosti, Silvia, additional, Murachelli, Silvia, additional, Musso, Maria, additional, Nardi, Michela, additional, Navarra, Assunta, additional, Nocioni, Martina, additional, Noto, Pasquale, additional, Noto, Roberto, additional, Oliva, Alessandra, additional, Onnis, Ilaria, additional, Ottou, Sandrine, additional, Palazzolo, Claudia, additional, Pallini, Emanuele, additional, Palombi, Giulio, additional, Pareo, Carlo, additional, Passeri, Virgilio, additional, Pelliccioni, Federico, additional, Penna, Giovanna, additional, Petrecchia, Antonella, additional, Petrone, Ada, additional, Pianura, Elisa, additional, Pinnetti, Carmela, additional, Pisciotta, Maria, additional, Piselli, Pierluca, additional, Pittalis, Silvia, additional, Pontarelli, Agostina, additional, Proietti, Costanza, additional, Puro, Vincenzo, additional, Ramazzini, Paolo Migliorisi, additional, Rianda, Alessia, additional, Rinonapoli, Gabriele, additional, Rosati, Silvia, additional, Rubino, Dorotea, additional, Rueca, Martina, additional, Ruggeri, Alberto, additional, Sacchi, Alessandra, additional, Sampaolesi, Alessandro, additional, Sanasi, Francesco, additional, Santagata, Carmen, additional, Scarabello, Alessandra, additional, Scarcia, Silvana, additional, Schininà, Vincenzo, additional, Scognamiglio, Paola, additional, Scorzolini, Laura, additional, Stazi, Giulia, additional, Strano, Giacomo, additional, Taibi, Chiara, additional, Taloni, Giorgia, additional, Nardi, Tetaj, additional, Tonnarini, Roberto, additional, Topino, Simone, additional, Tozzi, Martina, additional, Vairo, Francesco, additional, Vergori, Alessandra, additional, Vincenzi, Laura, additional, Visco-Comandini, Ubaldo, additional, Vita, Serena, additional, Vittozzi, Pietro, additional, Zaccarelli, Mauro, additional, and Zanetti, Antonella, additional

Published
2021
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12. Additional file 1: of Probiotics and infective endocarditis in patients with hereditary hemorrhagic telangiectasia: a clinical case and a review of the literature

Author

Evangelo Boumis, Capone, Alessandro, Galati, Vincenzo, Venditti, Carolina, and Petrosillo, Nicola

Abstract

Microbiology. Description of methods used to identify the microorganism isolated from blood culture, both by standard microbiology culture and by genetic analysis; minimum inhibitory concentrations (MICs) of the antibiotics used for testing. (DOCX 11 kb)

Published
2018
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13. Comparative evaluation of subtyping tools for surveillance of newly emerging HIV-1 strains

Author

Fabeni, Lavinia, Berno, Giulia, Fokam, Joseph, Bertoli, Ada, Alteri, Claudia, Gori, Caterina, Forbici, Federica, Takou, Desirã, Vergori, Alessandra, Zaccarelli, Mauro, Maffongelli, Gaetano, Borghi, Vanni, Latini, Alessandra, Pennica, Alfredo, Mastroianni, Claudio Maria, Montella, Francesco, Mussini, Cristina, Andreoni, Massimo, Antinori, Andrea, Perno, Carlo Federico, Santoro, Maria Mercedes, Armenia, Daniele, Bellocchi, Maria Concetta, Biddittu, Andrea, Bruni, Massimiliano, Buonomini, Anna Rita, Carioti, Luca, Ceccherini Silberstein, Francesca, Cerva, Carlotta, Cesta, Novella, Di Carlo, Domenico, Dori, Luca, Foroghi, Luca, Gentilotti, Elisa, Giannella, Sara, Guenci, Tania, Malagnino, Vincenzo, Ricciardi, Alessandra, Romani, Marzia, Salpini, Omina, Sarmati, Loredana, Scutari, Rossana, Serafini, Valentina, Sordillo, Pasquale, Stazi, Francesca, Stingone, Cristof, Svicher, Valentina, Teti, Elisabetta, Viscione, Magdalena, Abbate, Isabella, Acinapura, Rosa, Alba, Lucia, Ammassari, Adriana, Baldini, Franco, Bellagamba, Rita, Boumis, Evangelo, Capobianchi, Maria Rosaria, Carta, Stefania, Cicalini, Stefania, Continenza, Fabio, De Carli, Gabriella, D'Arrigo, Roberta, D'Offizi, Gianpiero, Fedele, Valentina, Galati, Vincenzo, Giannetti, Alberto, Girardi, Enrico, Grisetti, Susanna, Libertone, Raffaella, Liuzzi, Giuseppina, Lorenzini, Patrizia, Maddaluno, Rita, Mariano, Andrea, Navarra, Assunta, Nicastri, Emanuele, Nurra, Giuseppina, Orchi, Nicoletta, Palummieri, Antonio, Pinnetti, Carmela, Pittalis, Silvia, Pizzi, Daniele, Puro, Vincenzo, Sampaolesi, Alessandro, Sciarrone, Maria Rosaria, Scognamiglio, Paola, Sias, Catia, Visco Comandini, Ubaldo, Colafigli, Manuela, Cristaudo, Antonio, Giuliani, Massimo, Pacifici, Anna, Di Sora, Fiorella, Iebba, Filippo, Lichtner, Miriam, Marocco, Raffaella, Bernardi, Stefania, Anzalone, Enza, Bonaventura, Maria Elena, Marchili, Mauro, Pitorri, Antonella, Di Francesco, Luigi Falconi, Di Giammartino, Dante, Caterini, Antonio, Armignacco, Orlando, Mariani, Rinalda, Paoloni, Maurizio, Parruti, Giustino, Pieri, Alessandro, Sozio, Federica, Cellini, Antonio, Grimaldi, Alessandro, Mariani, Maurizio, Picchi, Giovanna, Gennari, William, Nanfack, Aubin J., Ndjolo, Alexis, and Torimiro, Judith N.

Subjects
0301 basic medicine, Microbiology (medical), Settore MED/17 - Malattie Infettive, Human immunodeficiency virus (HIV), Context (language use), HIV Infections, Biology, medicine.disease_cause, Sensitivity and Specificity, Genetic diversity, Comparative evaluation, 03 medical and health sciences, HIV Protease, Virology, Genotype, Circulating recombinant forms, HIV-1, Phylogeny, Subtypes, Subtyping automated tools, Databases, Genetic, medicine, Humans, CRFS, Automation, Laboratory, Base Sequence, Sequence Analysis, RNA, circulating recombinant forms, genetic diversity, phylogeny, subtypes, subtyping automated tools, microbiology, Gold standard (test), Subtyping, HIV Reverse Transcriptase, Molecular Typing, 030104 developmental biology, Epidemiological surveillance
Abstract

HIV-1 non-B subtypes/circulating recombinant forms (CRFs) are increasing worldwide. Since subtype identification can be clinically relevant, we assessed the added value in HIV-1 subtyping using updated molecular phylogeny (Mphy) and the performance of routinely used automated tools. Updated Mphy (2015 updated reference sequences), used as a gold standard, was performed to subtype 13,116 HIV-1 protease/reverse transcriptase sequences and then compared with previous Mphy (reference sequences until 2014) and with COMET, REGA, SCUEAL, and Stanford subtyping tools. Updated Mphy classified subtype B as the most prevalent (73.4%), followed by CRF02_AG (7.9%), C (4.6%), F1 (3.4%), A1 (2.2%), G (1.6%), CRF12_BF (1.2%), and other subtypes (5.7%). A 2.3% proportion of sequences were reassigned as different subtypes or CRFs because of misclassification by previous Mphy. Overall, the tool most concordant with updated Mphy was Stanford-v8.1 (95.4%), followed by COMET (93.8%), REGA-v3 (92.5%), Stanford-old (91.1%), and SCUEAL (85.9%). All the tools had a high sensitivity (≥98.0%) and specificity (≥95.7%) for subtype B. Regarding non-B subtypes, Stanford-v8.1 was the best tool for C, D, and F subtypes and for CRFs 01, 02, 06, 11, and 36 (sensitivity, ≥92.6%; specificity, ≥99.1%). A1 and G subtypes were better classified by COMET (92.3%) and REGA-v3 (98.6%), respectively. Our findings confirm Mphy as the gold standard for accurate HIV-1 subtyping, although Stanford-v8.1, occasionally combined with COMET or REGA-v3, represents an effective subtyping approach in clinical settings. Periodic updating of HIV-1 reference sequences is fundamental to improving subtype characterization in the context of an effective epidemiological surveillance of non-B strains.

Published
2017

16. Clostridium difficile infection among hospitalized HIV-infected individuals: epidemiology and risk factors: results from a case-control study (2002-2013)

Author

Di Bella, Stefano, primary, Friedrich, Alexander W, additional, García-Almodóvar, Esther, additional, Gallone, Maria Serena, additional, Taglietti, Fabrizio, additional, Topino, Simone, additional, Galati, Vincenzo, additional, Johnson, Emma, additional, D’Arezzo, Silvia, additional, and Petrosillo, Nicola, additional

Published
2015
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23. Decay of HIV Type 1 DNA and Development of Drug-Resistant Mutants in Patients with Primary HIV Type 1 Infection Receiving Highly Active Antiretroviral Therapy

Author

Riva, Elisabetta, primary, Pistello, Mauro, additional, Narciso, Pasquale, additional, D'Offizi, Gianpiero, additional, Isola, Patrizia, additional, Galati, Vincenzo, additional, Turriziani, Ombretta, additional, Tozzi, Valerio, additional, Vincenzi, Laura, additional, Dianzani, Ferdinando, additional, and Antonelli, Guido, additional

Published
2001
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25. Extra-cerebral severe infections associated with haemorrhagic hereditary telangiectasia (Rendu-Osler-Weber Disease): five cases and a review of the literature.

Author

Musso M, Capone A, Chinello P, Di Bella S, Galati V, Noto P, Taglietti F, Topino S, and Petrosillo N

Subjects
Aged, Female, Humans, Male, Middle Aged, Retrospective Studies, Severity of Illness Index, Bacterial Infections complications, Telangiectasia, Hereditary Hemorrhagic complications
Abstract

Hereditary haemorrhagic telangiectasia (HHT) is one of the most common autosomal dominant disorders and is characterized by genetically determined abnormalities of vascular structure. People affected by HHT are predisposed to severe infections such as cerebral abscesses, typical of patients with pulmonary arteriovenous malformations, and extra-cerebral infections such as bacteraemia, septic arthritis, osteomyelitis, hepatic abscesses, skin infections and infective endocarditis. We present a retrospective series of severe bacterial extra-cerebral infections in five patients affected by HHT, admitted to our Institute from January 2007 to June 2013. We also reviewed the literature of the last five years concerning infectious complications in people affected by HHT. Our study shows that HHT patients with infectious complications exclusively localized in extra-cerebral sites are usually fragile, old and affected by comorbidities. Moreover, we recognized a trend of Staphylococcus aureus (SA) severe infection recurrence in such patients, both in our series and in the literature. In our opinion these results suggest the need to evaluate the possible benefits of SA nasal colonization screening and decolonization in such patients.

Published
2014

26. Impact of hepatitis C virus infection on lifestyle.

Author

Scognamiglio P, Galati V, Navarra A, Longo MA, Aloisi MS, Antonini MG, Puoti M, Almasio PL, Ippolito G, and Girardi E

Subjects
Adult, Aged, Aged, 80 and over, Alcohol Drinking, Diet, Female, Health Surveys, Humans, Italy, Male, Middle Aged, Smoking, Sports, Health Behavior, Hepatitis C physiopathology, Hepatitis C psychology, Life Style
Abstract

Aim: To evaluate the impact of the diagnosis of hepatitis C infection on lifestyle habits such as smoking, drinking, sports activity and diet., Methods: A self-administered, anonymous questionnaire was offered to out-patients with HCV infection consecutively attending three clinical centres in Italy., Results: Of the 275 respondents, 62.2% (171) were male. Mean age was 51 (range 20-80) years. Overall, after the diagnosis of hepatitis C, 74.5% of drinkers had modified (giving up or reducing) alcohol consumption, 21.3% of smokers had modified their habits and 32.3% of subjects who reported sports activity had either stopped or reduced frequency of activity or chose a less fatiguing sport. Sixty-four percent of the respondents reported having modified their diet, most of them on physician's advice., Conclusion: After the diagnosis of hepatitis C many patients correctly modified their alcohol consumption and a minority their smoking habits. The reason for reported changes in diet and physical activity needs further investigations.

Published
2007
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