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2. Spatio-temporal variations in chemical pollutants found among urban deposits match changes in thiopurine S-methyltransferase-harboring bacteria tracked by the tpm metabarcoding approach

Author

Aigle, Axel, Colin, Yannick, Bouchali, Rayan, Bourgeois, Emilie, Marti, Romain, Ribun, Sébastien, Marjolet, Laurence, Pozzi, Adrien C.M., Misery, Boris, Colinon, Céline, Bernardin-Souibgui, Claire, Wiest, Laure, Blaha, Didier, Galia, Wessam, and Cournoyer, Benoit

Published
2021
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3. Trace contaminants in the environmental assessment of organic waste recycling in agriculture: Gaps between methods and knowledge

Author

Avadí, Angel, primary, Benoit, Pierre, additional, Bravin, Matthieu N., additional, Cournoyer, Benoit, additional, Feder, Frédéric, additional, Galia, Wessam, additional, Garnier, Patricia, additional, Haudin, Claire-Sophie, additional, Legros, Samuel, additional, Mamy, Laure, additional, Nazaret, Sylvie, additional, Patureau, Dominique, additional, Pot, Valérie, additional, Vieublé Gonod, Laure, additional, Wassenaar, Tom, additional, and Doelsch, Emmanuel, additional

Published
2022
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4. Occurrence of 40 sanitary indicators in French digestates derived from different anaerobic digestion processes and raw organic wastes from agricultural and urban origin.

Author

Wybraniec, Caroline, Cournoyer, Benoit, Moussard, Cécile, Beaupère, Marion, Lusurier, Léa, Leriche, Françoise, Fayolle, Karine, Sertillanges, Nicolas, Haudin, Claire-Sophie, Houot, Sabine, Patureau, Dominique, Gagne, Geneviève, and Galia, Wessam

Subjects
MOBILE genetic elements, POLLUTANTS, AGRICULTURAL wastes, ORGANIC wastes, CATTLE manure, ENTEROCOCCUS, CLOSTRIDIUM perfringens, MICROPOLLUTANTS, TRICLOSAN
Abstract

This study investigated the sanitary quality of digestates resulting from the mesophilic anaerobic digestion (AD) of urban and agricultural organic wastes (OWs). 40 sanitary indicators, including pathogenic bacteria, antimicrobial resistance genes, virulence factor genes, and mobile genetic elements were evaluated using real-time PCR and/or droplet digital PCR. 13 polycyclic aromatic hydrocarbons (PAHs) and 13 pharmaceutical products (PHPs) were also measured. We assessed agricultural OWs from three treatment plants to study the effect of different AD processes (feeding mode, number of stages, pH), and used three laboratory-scale reactors to study the effect of different feedsupplies (inputs). The lab-scale reactors included: Lab1 fed with 97% activated sludge (urban waste) and 3% cow manure; Lab2 fed with 85% sludge-manure mixture supplemented with 15% wheat straw (WS); and Lab3 fed with 81% sludgemanure mixture, 15% WS, and 4% zeolite powder. Activated sludge favored the survival of the food-borne pathogens Clostridium perfringens and Bacillus cereus, carrying the toxin-encoding genes cpe and ces, respectively. Globally, the reactors fed with fecal matter supplemented with straw (Lab2) or with straw and zeolite (Lab3) had a higher hygienization efficiency than the reactor fed uniquely with fecal matter (Lab1). Three pathogenic bacteria (Enterococcus faecalis, Enterococcus faecium, and Mycobacterium tuberculosis complex), a beta-lactam resistance gene (blaTEM), and three mobile genetic elements (intI1, intI2, and IS26) were significantly decreased in Lab2 and Lab3. Moreover, the concentrations of 11 PAHs and 11 PHPs were significantly lower in Lab2 and Lab3 samples than in Lab1 samples. The high concentrations of micropollutants, such as triclosan, found in Lab1, could explain the lower hygienization efficiency of this reactor. Furthermore, the batch-fed reactor had a more efficient hygienization effect than the semi-continuous reactors, with complete removal of the ybtA gene, which is involved in the production of the siderophore yersiniabactin, and significant reduction of intI2 and tetO. These data suggest that it is essential to control the level of chemical pollutants in raw OWs to optimize the sanitary quality of digestates, and that adding co-substrate, such as WS, may overcome the harmful effect of pollutants. [ABSTRACT FROM AUTHOR]

Published
2024
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12. Identification of Streptococcus thermophilus Genes Specifically Expressed under Simulated Human Digestive Conditions Using R-IVET Technology

Author

Uriot, Ophélie, Kebouchi, Mounira, Lorson, Emilie, Galia, Wessam, Denis, Sylvain, Chalancon, Sandrine, Hafeez, Zeeshan, Roux, Emeline, Genay, Magali, BLANQUET-DIOT, Stéphanie, Dary-Mourot, Annie, Composés Alimentaires : Biofonctionnalités et risques Neurotoxiques (CALBINOTOX), Université de Lorraine (UL), Microbiologie Environnement Digestif Santé (MEDIS), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Clermont Auvergne (UCA), Laboratoire d'Ecologie Microbienne - UMR 5557 (LEM), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Ecole Nationale Vétérinaire de Lyon (ENVL)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Scalable, Optimized and Parallel Algorithms for Genomics (GenScale), Inria Rennes – Bretagne Atlantique, Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria)-GESTION DES DONNÉES ET DE LA CONNAISSANCE (IRISA-D7), Institut de Recherche en Informatique et Systèmes Aléatoires (IRISA), Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Université de Bretagne Sud (UBS)-École normale supérieure - Rennes (ENS Rennes)-Institut National de Recherche en Informatique et en Automatique (Inria)-CentraleSupélec-Centre National de la Recherche Scientifique (CNRS)-IMT Atlantique (IMT Atlantique), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)-Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)-Institut de Recherche en Informatique et Systèmes Aléatoires (IRISA), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Université de Bretagne Sud (UBS)-École normale supérieure - Rennes (ENS Rennes)-CentraleSupélec-Centre National de la Recherche Scientifique (CNRS)-IMT Atlantique (IMT Atlantique), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT), Ophélie Uriot (O.U.) and Mounira Kebouchi (M.K.) were the recipients of a PhD fellowship from the Ministère de l’Enseignement Supérieur, de la Recherche, et de l’Innovation., Université de Bretagne Sud (UBS)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National de Recherche en Informatique et en Automatique (Inria)-École normale supérieure - Rennes (ENS Rennes)-Centre National de la Recherche Scientifique (CNRS)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-CentraleSupélec-IMT Atlantique Bretagne-Pays de la Loire (IMT Atlantique), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)-Université de Bretagne Sud (UBS)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), and Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-École normale supérieure - Rennes (ENS Rennes)-Centre National de la Recherche Scientifique (CNRS)-Université de Rennes 1 (UR1)

Subjects
intestinal microbiota, R-IVET, S. thermophilus, adhesion, TIM-1 system, QH301-705.5, [SDV]Life Sciences [q-bio], Biology (General)
Abstract

International audience; Despite promising health effects, the probiotic status of Streptococcus thermophilus, a lactic acid bacterium widely used in dairy industry, requires further documentation of its physiological status during human gastrointestinal passage. This study aimed to apply recombinant-based in vivo technology (R-IVET) to identify genes triggered in a S. thermophilus LMD-9 reference strain under simulated digestive conditions. First, the R-IVET chromosomal cassette and plasmid genomic library were designed to positively select activated genes. Second, recombinant clones were introduced into complementary models mimicking the human gut, the Netherlands Organization for Applied Scientific Research (TNO) gastrointestinal model imitating the human stomach and small intestine, the Caco-2 TC7 cell line as a model of intestinal epithelium, and anaerobic batch cultures of human feces as a colon model. All inserts of activated clones displayed a promoter activity that differed from one digestive condition to another. Our results also showed that S. thermophilus adapted its metabolism to stressful conditions found in the gastric and colonic competitive environment and modified its surface proteins during adhesion to Caco-2 TC7 cells. Activated genes were investigated in a collection of S. thermophilus strains showing various resistance levels to gastrointestinal stresses, a first stage in the identification of gut resistance markers and a key step in probiotic selection.

Published
2021
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13. Relative weight of organic waste origin on compost and digestate 16S rRNA gene bacterial profilings and related functional inferences

Author

Aigle, Axel, Bourgeois, Emilie, Marjolet, Laurence, Houot, Sabine, Patureau, Dominique, Doelsch, Emmanuel, Cournoyer, Benoit, Galia, Wessam, Aigle, Axel, Bourgeois, Emilie, Marjolet, Laurence, Houot, Sabine, Patureau, Dominique, Doelsch, Emmanuel, Cournoyer, Benoit, and Galia, Wessam

Abstract

Even though organic waste (OW) recycling via anaerobic digestion (AD) and composting are increasingly used, little is known about the impact of OW origin (fecal matters and food and vegetable wastes) on the end products' bacterial contents. The hypothesis of a predictable bacterial community structure in the end products according to the OW origin was tested. Nine OW treatment plants were selected to assess the genetic structure of bacterial communities found in raw OW according to their content in agricultural and urban wastes and to estimate their modifications through AD and composting. Two main bacterial community structures among raw OWs were observed and matched a differentiation according to the occurrences of urban chemical pollutants. Composting led to similar 16S rRNA gene OTU profiles whatever the OW origin. With a significant shift of about 140 genera (representing 50% of the bacteria), composting was confirmed to largely shape bacterial communities toward similar structures. The enriched taxa were found to be involved in detoxification and bioremediation activities. This process was found to be highly selective and favorable for bacterial specialists. Digestates showed that OTU profiles differentiated into two groups according to their relative content in agricultural (manure) and urban wastes (mainly activated sludge). About one third of the bacterial taxa was significantly affected by AD. In digestates of urban OW, this sorting led to an enrichment of 32 out of the 50 impacted genera, while for those produced from agricultural or mixed urban/agricultural OW (called central OW), a decay of 54 genera over 60 was observed. Bacteria from activated sludge appeared more fit for AD than those of other origins. Functional inferences showed AD enriched genera from all origins to share similar functional traits, e.g., chemoheterotrophy and fermentation, while being often taxonomically distinct. The main functional traits among the dominant genera in activated s

Published
2021

15. Multi-targeted properties of the probiotic saccharomyces cerevisiae CNCM I-3856 against enterotoxigenic escherichia coli (ETEC) H10407 pathogenesis across human gut models

Author

Roussel, Charlène, primary, De Paepe, Kim, additional, Galia, Wessam, additional, de Bodt, Jana, additional, Chalancon, Sandrine, additional, Denis, Sylvain, additional, Leriche, Françoise, additional, Vandekerkove, Pascal, additional, Ballet, Nathalie, additional, Blanquet-Diot, Stéphanie, additional, and Van de Wiele, Tom, additional

Published
2021
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16. Additional file 1 of Spatial and temporal modulation of enterotoxigenic E. coli H10407 pathogenesis and interplay with microbiota in human gut models

Author

Roussel, Charlène, Paepe, Kim De, Galia, Wessam, Bodt, Jana De, Chalancon, Sandrine, Leriche, Françoise, Ballet, Nathalie, Denis, Sylvain, Alric, Monique, Wiele, Tom Van De, and Blanquet-Diot, Stéphanie

Subjects
digestive, oral, and skin physiology, digestive system
Abstract

Additional file 1: Fig. S1. [Global ETEC survival percentages in the cumulated ileal effluents of the TIM-1]. Fig. S2. [Live/Dead flow cytometry for accurate determination of ETEC membrane physiology in the TIM-1 gastric and ileal effluents]. Fig. S3. [Spearman correlation between virulence gene expression, LT toxin production and/or ETEC survival in the TIM-1 and M-SHIME]. Fig. S4. [Phylum and species levels microbial community composition of the luminal gut regions]. Fig. S5. [Phylum and species levels microbial community composition of the mucosal gut regions]. Fig. S6. [Gut microbes concentration in the ileum and ascending colon lumen determined by total flora 16S rRNA gene quantification]. Fig. S7. [Simpson alpha diversity index over time for the six donors according to the luminal (L) and mucosal (M) gut regions]. Fig. S8. [db-RDA triplots showing the relationship of A) gut regions, B) donors and C) period pre- vs post-infection as explanatory variables to the microbial community structure at genus level]. Fig. S9. [db-RDA triplots showing the effect of ETEC pre- and post-infection on distinct microbial genera according to the gut regions]. Fig. S10. [SCFA concentrations (mM) in the ileum (ILE) and ascending colon (ASC) compartments of the M-SHIME]. Fig. S11. [Spearman correlations between the main SCFA produced in the ascending colon and ETEC survival, LT toxin production, and expression of virulence genes encoding for the enterotoxins]. Fig. S12. [High reproducibility in SCFA concentrations and microbiota composition between replicates from a same donor in a separate SHIME experiment]. Table S1. [ETEC intracellular pH (pHi) in the TIM-1 system]. Table S2. [ETEC membrane potential in the TIM-1 system]. Table S3. [Log2 fold changes in virulence genes expression in the TIM-1]. Table S4. [Log2 fold changes in virulence genes expression in the M-SHIME]. Table S5. [Parameters of the TIM-1 and M-SHIME systems]. Table S6. [General characteristic of the fecal donors involved in the M-SHIME experiments]. Table S7. [Static in vitro gastro-jejunal digestion procedure]. Table S8. [ETEC primers used in the study]. Table S9. [RDP Seqmatch and NCBI BLAST results for the most abundant species and/or species of interest in the M-SHIME].

Published
2020
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17. Des bactéries pathogènes opportunistes en ville

Author

Cournoyer, Benoit, Marti, Romain, Bourgeois, Emilie, Galia, Wessam, Pillet, Lauriane, Laboratoire d'Ecologie Microbienne - UMR 5557 (LEM), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Ecole Nationale Vétérinaire de Lyon (ENVL)

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2019

19. Additional file 7: Table S7. of Strand-specific transcriptomes of Enterohemorrhagic Escherichia coli in response to interactions with ground beef microbiota: interactions between microorganisms in raw meat

Author

Galia, Wessam, Francoise Leriche, StĂŠphane Cruveiller, Garnier, Cindy, Navratil, Vincent, Dubost, Audrey, StĂŠphanie Blanquet-Diot, and Thevenot-Sergentet, Delphine

Subjects
stomatognathic diseases, fluids and secretions, digestive system
Abstract

Others down-regulated genes in Escherichia coli O157:H7 EDL933 in samples with microbiota compared to those without microbiota. (DOC 36 kb)

Published
2017
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20. Additional file 2: Table S2. of Strand-specific transcriptomes of Enterohemorrhagic Escherichia coli in response to interactions with ground beef microbiota: interactions between microorganisms in raw meat

Author

Galia, Wessam, Francoise Leriche, Cruveiller, Stéphane, Garnier, Cindy, Navratil, Vincent, Dubost, Audrey, Blanquet-Diot, Stéphanie, and Thevenot-Sergentet, Delphine

Subjects
fluids and secretions, bacteria
Abstract

Population over time of two strains of enterohemorrhagic Escherichia coli (O157:H7 EDL933 and O26:H11 21,765) grown in ground beef held at 12 °C and prepared from the outer or inner part of the muscle. (DOC 30 kb)

Published
2017
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21. Additional file 4: Table S4. of Strand-specific transcriptomes of Enterohemorrhagic Escherichia coli in response to interactions with ground beef microbiota: interactions between microorganisms in raw meat

Author

Galia, Wessam, Francoise Leriche, StĂŠphane Cruveiller, Garnier, Cindy, Navratil, Vincent, Dubost, Audrey, StĂŠphanie Blanquet-Diot, and Thevenot-Sergentet, Delphine

Subjects
food and beverages
Abstract

Summary of enterohemorrhagic Escherichia coli cDNA samples sequenced in ground beef with or without microbiota (DOC 41 kb)

Published
2017
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22. Additional file 3: Table S3. of Strand-specific transcriptomes of Enterohemorrhagic Escherichia coli in response to interactions with ground beef microbiota: interactions between microorganisms in raw meat

Author

Galia, Wessam, Francoise Leriche, StĂŠphane Cruveiller, Garnier, Cindy, Navratil, Vincent, Dubost, Audrey, StĂŠphanie Blanquet-Diot, and Thevenot-Sergentet, Delphine

Subjects
fluids and secretions, bacteria
Abstract

Genus level distribution of sequences based on the 16S rRNA gene libraries constructed from a ground beef sample with natural microflora (A) and ground beef samples inoculated with E. coli O26:H11 21,765 (B) or E. coli O157:H7 EDL933 (C) strains. (DOC 55 kb)

Published
2017
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23. Additional file 8: Table S8. of Strand-specific transcriptomes of Enterohemorrhagic Escherichia coli in response to interactions with ground beef microbiota: interactions between microorganisms in raw meat

Author

Galia, Wessam, Francoise Leriche, StĂŠphane Cruveiller, Garnier, Cindy, Navratil, Vincent, Dubost, Audrey, StĂŠphanie Blanquet-Diot, and Thevenot-Sergentet, Delphine

Subjects
stomatognathic diseases, fluids and secretions, digestive system
Abstract

Discarded up-regulated genes in Escherichia coli O157:H7 EDL933 in samples with microbiota compared to those without microbiota. (DOC 62 kb)

Published
2017
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24. Additional file 6: Table S6. of Strand-specific transcriptomes of Enterohemorrhagic Escherichia coli in response to interactions with ground beef microbiota: interactions between microorganisms in raw meat

Author

Galia, Wessam, Francoise Leriche, StĂŠphane Cruveiller, Garnier, Cindy, Navratil, Vincent, Dubost, Audrey, StĂŠphanie Blanquet-Diot, and Thevenot-Sergentet, Delphine

Subjects
stomatognathic diseases, fluids and secretions, bacteria, bacterial infections and mycoses, digestive system
Abstract

Discarded up-regulated genes in Escherichia coli O26:H11 21,765 in samples with microbiota compared to those without microbiota. (DOC 51 kb)

Published
2017
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25. Additional file 5: Table S5. of Strand-specific transcriptomes of Enterohemorrhagic Escherichia coli in response to interactions with ground beef microbiota: interactions between microorganisms in raw meat

Author

Galia, Wessam, Francoise Leriche, StĂŠphane Cruveiller, Garnier, Cindy, Navratil, Vincent, Dubost, Audrey, StĂŠphanie Blanquet-Diot, and Thevenot-Sergentet, Delphine

Abstract

Others down-regulated genes in Escherichia coli O26:H11 21,765 in samples with microbiota compared to those without microbiota. (DOC 95 kb)

Published
2017
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27. Increased EHEC survival and virulence gene expression indicate an enhanced pathogenicity upon simulated pediatric gastrointestinal conditions

Author

Roussel, Charlène, primary, Cordonnier, Charlotte, additional, Galia, Wessam, additional, Le Goff, Olivier, additional, Thévenot, Jonathan, additional, Chalancon, Sandrine, additional, Alric, Monique, additional, Thevenot-Sergentet, Delphine, additional, Leriche, Francoise, additional, Van de Wiele, Tom, additional, Livrelli, Valérie, additional, and Blanquet-Diot, Stéphanie, additional

Published
2016
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28. Milk Fat Globules Hamper Adhesion of Enterohemorrhagic Escherichia coli to Enterocytes: In Vitro and in Vivo Evidence.

Author

Montel, Marie-Christine, Douëllou, Thomas, Galia, Wessam, Kerangart, Stéphane, Bouvier, Marion, Sergentet-Thevenot, Delphine, Marchal, Thierry, Milhau, Nadège, Buff, Samuel, and Bastien, Renaud

Subjects
ESCHERICHIA coli, ENTEROCYTES
Abstract

Enterohemorrhagic Escherichia coli (EHEC; E. coli) are food-borne agents associated with gastroenteritis, enterocolitis, bloody diarrhea and the hemolytic-uremic syndrome (HUS). Bovine milk glycans have been shown to contain oligosaccharides which are similar to host epithelial cell receptors and can therefore prevent bacterial adhesion. This study aimed to describe interactions between EHEC O157:H7 EDL933 and O26:H11 21765 and milk fat globules (MFGs) in raw milk and raw milk cheese, and the impact of MFGs on EHEC strains adhesion to the intestinal tract in vitro and in vivo. Both EHEC serotypes clearly associated with native bovine MFGs and significantly limited their adhesion to a co-culture of intestinal cells. The presence of MFGs in raw milk cheese had two effects on the adhesion of both EHEC serotypes to the intestinal tracts of streptomycin-treated mice. First, it delayed and reduced EHEC excretion in mouse feces for both strains. Second, the prime implantation site for both EHEC strains was 6 cm more proximal in the intestinal tracts of mice fed with contaminated cheese containing less than 5% of fat than in those fed with contaminated cheese containing 40% of fat. Feeding mice with 40% fat cheese reduced the intestinal surface contaminated with EHEC and may therefore decrease severity of illness. [ABSTRACT FROM AUTHOR]

Published
2018
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29. Use of the dynamic gastro-intestinal model TIM to explore the survival of the yogurt bacterium Streptococcus thermophilus and the metabolic activities induced in the simulated human gut

Author

Uriot, Ophélie, primary, Galia, Wessam, additional, Awussi, Ahoefa Ablavi, additional, Perrin, Clarisse, additional, Denis, Sylvain, additional, Chalancon, Sandrine, additional, Lorson, Emilie, additional, Poirson, Chantal, additional, Junjua, Maira, additional, Le Roux, Yves, additional, Alric, Monique, additional, Dary, Annie, additional, Blanquet-Diot, Stéphanie, additional, and Roussel, Yvonne, additional

Published
2016
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30. Correction for Galia et al., Genome Sequence and Annotation of a Human Infection Isolate of Escherichia coli O26:H11 Involved in a Raw Milk Cheese Outbreak

Author

Galia, Wessam, primary, Mariani-Kurkdjian, Patricia, additional, Bastien, Sylvere, additional, Loukiadis, Estelle, additional, Blanquet-Diot, Stéphanie, additional, Leriche, Françoise, additional, Brugère, Hubert, additional, Shima, Ayaka, additional, Oswald, Eric, additional, Cournoyer, Benoit, additional, and Thevenot-Sergentet, Delphine, additional

Published
2015
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32. Variability of the proteolytic properties in Streptococcus thermophilus

Author

Galia, Wessam, Perrin, Clarisse, Jameh, Nawara, Genay, Magali, Dary, Annie, Unité de Recherches Animal et Fonctionnalités des Produits Animaux (URAFPA), and Institut National de la Recherche Agronomique (INRA)-Université de Lorraine (UL)

Subjects
bactérie, VARIABILITÉ, streptococcus thermophilus, caséine, protéine, [SDV]Life Sciences [q-bio], souche, PROPRIÉTÉ ACIDIFIANTE, [INFO]Computer Science [cs], PROPRIÉTÉ PROTÉOLYTIQUE, lait
Published
2009

33. Etude de la variabilité du potentiel protéolytique de paroi chez Streptococcus thermophilus: Caractérisation de la variabilité du gène prtS

Author

Galia, Wessam, Dary, Annie, Perrin, Clarisse, Unité de Recherches Animal et Fonctionnalités des Produits Animaux (URAFPA), Institut National de la Recherche Agronomique (INRA)-Université de Lorraine (UL), and ProdInra, Migration

Subjects
[SDV] Life Sciences [q-bio], PROPRIETE PROTEOLYTIQUE, VARIABILITE, [SDV]Life Sciences [q-bio], [INFO]Computer Science [cs], [INFO] Computer Science [cs], ComputingMilieux_MISCELLANEOUS, GENE PRTS
Abstract

National audience

Published
2009

34. Genome Sequence and Annotation of a Human Infection Isolate of Escherichia coli O26:H11 Involved in a Raw Milk Cheese Outbreak

Author

Galia, Wessam, primary, Mariani-Kurkdjian, Patricia, additional, Loukiadis, Estelle, additional, Blanquet-Diot, Stéphanie, additional, Leriche, Françoise, additional, Brugère, Hubert, additional, Shima, Ayaka, additional, Oswald, Eric, additional, Cournoyer, Benoit, additional, and Thevenot-Sergentet, Delphine, additional

Published
2015
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35. Multi-targeted properties of the probiotic saccharomyces cerevisiaeCNCM I-3856 against enterotoxigenic escherichia coli(ETEC) H10407 pathogenesis across human gut models

Author

Roussel, Charlène, De Paepe, Kim, Galia, Wessam, de Bodt, Jana, Chalancon, Sandrine, Denis, Sylvain, Leriche, Françoise, Vandekerkove, Pascal, Ballet, Nathalie, Blanquet-Diot, Stéphanie, and Van de Wiele, Tom

Abstract

ABSTRACTEnterotoxigenic Escherichia coli(ETEC) is one of the most common causes of acute traveler’s diarrhea. Adhesins and enterotoxins constitute the major ETEC virulence traits. With the dramatic increase in antibiotic resistance, probiotics are considered a wholesome alternative to prevent or treat ETEC infections. Here, we examined the antimicrobial properties of the probiotic Saccharomyces cerevisiaeCNCM I-3856 against ETEC H10407 pathogenesis upon co-administration in the TNO gastrointestinal Model (TIM-1), simulating the physicochemical and enzymatic conditions of the human upper digestive tract and preventive treatment in the Mucosal Simulator of the Human Intestinal Microbial Ecosystem (M-SHIME), integrating microbial populations of the ileum and ascending colon. Interindividual variability was assessed by separate M-SHIME experiments with microbiota from six human individuals. The probiotic did not affect ETEC survival along the digestive tract. However, ETEC pathogenicity was significantly reduced: enterotoxin encoding virulence genes were repressed, especially in the TIM-1 system, and a lower enterotoxin production was noted. M-SHIME experiments revealed that 18-days probiotic treatment stimulate the growth of Bifidobacteriumand Lactobacillusin different gut regions (mucosal and luminal, ileum and ascending colon) while a stronger metabolic activity was noted in terms of short-chain fatty acids (acetate, propionate, and butyrate) and ethanol production. Moreover, the probiotic pre-treated microbiota displayed a higher robustness in composition following ETEC challenge compared to the control condition. We thus demonstrated the multi-inhibitory properties of the probiotic S. cerevisiaeCNCM I-3856 against ETEC in the overall simulated human digestive tract, regardless of the inherent variability across individuals in the M-SHIME.

Published
2021
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36. Identification of Streptococcus thermophilus Genes Specifically Expressed under Simulated Human Digestive Conditions Using R-IVET Technology.

Author

Uriot O, Kebouchi M, Lorson E, Galia W, Denis S, Chalancon S, Hafeez Z, Roux E, Genay M, Blanquet-Diot S, and Dary-Mourot A

Abstract

Despite promising health effects, the probiotic status of Streptococcus thermophilus, a lactic acid bacterium widely used in dairy industry, requires further documentation of its physiological status during human gastrointestinal passage. This study aimed to apply recombinant-based in vivo technology (R-IVET) to identify genes triggered in a S. thermophilus LMD-9 reference strain under simulated digestive conditions. First, the R-IVET chromosomal cassette and plasmid genomic library were designed to positively select activated genes. Second, recombinant clones were introduced into complementary models mimicking the human gut, the Netherlands Organization for Applied Scientific Research (TNO) gastrointestinal model imitating the human stomach and small intestine, the Caco-2 TC7 cell line as a model of intestinal epithelium, and anaerobic batch cultures of human feces as a colon model. All inserts of activated clones displayed a promoter activity that differed from one digestive condition to another. Our results also showed that S. thermophilus adapted its metabolism to stressful conditions found in the gastric and colonic competitive environment and modified its surface proteins during adhesion to Caco-2 TC7 cells. Activated genes were investigated in a collection of S. thermophilus strains showing various resistance levels to gastrointestinal stresses, a first stage in the identification of gut resistance markers and a key step in probiotic selection.

Published
2021
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