25 results on '"Galicia-Garcia, U."'
Search Results
2. Evidence of novel APO B gene complex allele causing familial hypercholesterolaemia
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Palma, D., primary, Cardiero, G., additional, Flagiello, C., additional, Galicia-Garcia, U., additional, Larrea, A., additional, Di Taranto, M.D., additional, Martin, C., additional, Iannuzzo, G., additional, Di Minno, M.N.D., additional, Pipolo, A., additional, and Fortunato, G., additional
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- 2022
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3. Boosting cholesterol efflux from foam cells by sequential administration of RHDL to deliver microRNA and to remove cholesterol in a triple-cell two-dimensional atherosclerosis model
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Benslaiman, S. Jebari, primary, Uribe, K.B., additional, Benito-Vicente, A., additional, Galicia-Garcia, U., additional, Larrea, A., additional, Alloza, I., additional, Vandenbroeck, K., additional, and Martín, C., additional
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- 2022
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4. Silencing of PCSK9 by SIRNA-functionalized RHDL as tool to upregulate LDLR expression in hepatocytes
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Larrea, A., primary, Benslaiman, S. Jebari, additional, Galicia-Garcia, U., additional, Uribe, K.B., additional, Benito-Vicente, A., additional, and Martin, C., additional
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- 2022
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5. MLb-LDLr: A Machine Learning Model for Predicting the Pathogenicity of LDLr Missense Variants
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Larrea-Sebal, A., Benito-Vicente, A., Fernandez-Higuero, J.A., Jebari-Benslaiman, S., Galicia-Garcia, U., Uribe, K.B., Cenarro, A., Ostolaza, H., Civeira, F., Arrasate, S., González-Díaz, H., Martín, C., Eusko Jaurlaritza, Universidad del País Vasco, and Fundación Biofísica Bizkaia
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Machine learning software ,AUROC, area under the receiver operating curve ,Familial hypercholesterolemia ,nutritional and metabolic diseases ,LDL receptor ,LNN, linear neural networks ,ESEA, Excel Solver Evolutionary algorithm ,ANN, artificial neural network ,FH, familial hypercholesterolemia ,LDLr, low-density lipoprotein receptor ,UTR, untranslated region ,RBF, radial basis function ,MLb-LDLr, machine-learning–based low-density lipoprotein receptor software ,LDL, low-density lipoprotein ,Pathogenicity ,lipids (amino acids, peptides, and proteins) ,EGS, expert-guided selection ,ML, machine learning ,MLP, multilayer perceptron ,Prediction ,LDA, linear discriminant analysis - Abstract
Untreated familial hypercholesterolemia (FH) leads to atherosclerosis and early cardiovascular disease. Mutations in the low-density lipoprotein receptor (LDLr) gene constitute the major cause of FH, and the high number of mutations already described in the LDLr makes necessary cascade screening or in vitro functional characterization to provide a definitive diagnosis. Implementation of high-predicting capacity software constitutes a valuable approach for assessing pathogenicity of LDLr variants to help in the early diagnosis and management of FH disease. This work provides a reliable machine learning model to accurately predict the pathogenicity of LDLr missense variants with specificity of 92.5% and sensitivity of 91.6%., This study was supported by grants from the Basque Government (Cesar Martin, Grupos Consolidados IT-1264-19). Mr Larrea-Sebal was supported by a FPI grant from Gobierno Vasco (2019–2020). Dr Benito-Vicente was supported by Programa de especialización de Personal Investigador Doctor en la UPV/EHU (2019) 2019-2020. Dr Galicia-Garcia was supported by Fundación Biofísica Bizkaia. Ms Jebari-Benslaiman was supported by grant PIF (2017–2018), Gobierno Vasco.
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- 2021
6. Replacement of cysteine at position 46 in the first cysteine-rich repeat of the LDL receptor impairs apolipoprotein recognition
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Benito-Vicente, A., primary, Uribe, K. B., additional, Siddiqi, H., additional, Jebari, S., additional, Galicia-Garcia, U., additional, Larrea-Sebal, A., additional, Cenarro, A., additional, Stef, M., additional, Ostolaza, H., additional, Civeira, F., additional, Palacios, L., additional, and Martin, C., additional
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- 2018
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7. Variable reductions in apolipoprotein (a) [Apo(a)] isoforms concentrations after lipapheresis treatment in patients with isolated hyper lipoprotein (a) [Lp(a)]
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Lambert, G., primary, Ramin-mangata, J., additional, Blanchard, V., additional, Garcia Nafria, J., additional, Galicia-Garcia, U., additional, Croyal, M., additional, and Martin, C., additional
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- 2018
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8. Functional characterization and classification of frequent variants located in the ldlr beta-propeller
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Galicia García, U., Benito-vicente, A., Uribe, K.B., Jebari, S., Palacios, L., Stef, M., Ostolaza, H., and Martin, C.
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- 2018
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9. The Arg499His gain-of-function mutation in the C-terminal domain of PCSK9
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Unai Galicia-Garcia, Asier Benito-Vicente, F. Javier Nóvoa, Shifa Jebari, Mauro Boronat, Maria Donata Di Taranto, Itziar Lamiquiz-Moneo, Kepa B. Uribe, Ana M. Wägner, César Martín, Fernando Civeira, Rosa M. Sánchez-Hernández, Asier Larrea-Sebal, Giuliana Fortunato, Sanchez-Hernandez, R. M., Di Taranto, M. D., Benito-Vicente, A., Uribe, K. B., Lamiquiz-Moneo, I., Larrea-Sebal, A., Jebari, S., Galicia-Garcia, U., Novoa, F. J., Boronat, M., Wagner, A. M., Civeira, F., Martin, C., and Fortunato, G.
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0301 basic medicine ,Heterozygote ,Apolipoprotein B ,Familial hypercholesterolemia ,030204 cardiovascular system & hematology ,Arginine ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Protein Domains ,Genetic ,medicine ,Humans ,Histidine ,Dyslipemia ,Functional assay ,Child ,Family Health ,biology ,Cholesterol ,PCSK9 ,Cell Membrane ,HEK 293 cells ,Hep G2 Cells ,Middle Aged ,Lipid ,medicine.disease ,Proprotein convertase ,Molecular biology ,Culture Media ,Pedigree ,HEK293 Cells ,030104 developmental biology ,Italy ,Receptors, LDL ,chemistry ,Spain ,Gain of Function Mutation ,LDL receptor ,biology.protein ,Kexin ,Female ,lipids (amino acids, peptides, and proteins) ,Gene expression ,Proprotein Convertase 9 ,Cardiology and Cardiovascular Medicine ,Mutations - Abstract
Background and aims Familial hypercholesterolemia (FH) is a monogenic disease characterized by high levels of low-density lipoprotein cholesterol and premature atherosclerotic cardiovascular disease. FH is caused by loss of function mutations in genes encoding LDL receptor (LDLR), and Apolipoprotein B (APOB) or gain of function (GOF) mutations in proprotein convertase subtilisin/kexin type 9 (PCSK9). In this study, we identified a novel variant in PCSK9, p.(Arg499His), located in the C-terminal domain, in two unrelated FH patients from Spain and Italy. Methods We studied familial segregation and determined variant activity in vitro. Results We determined PCSK9 expression, secretion and activity of the variant in transfected HEK293 cells; extracellular activity of the recombinant p.(Arg499His) PCSK9 variant in HEK 293 and HepG2 cells; PCSK9 affinity to the LDL receptor at neutral and acidic pH; the mechanism of action of the p.(Arg499His) PCSK9 variant by co-transfection with a soluble construct of the LDL receptor and by determining total PCSK9 intracellular accumulation when endosomal acidification is impaired and when an excess of soluble LDLr is present in the culture medium. Our results show high LDL-C concentrations and FH phenotype in p.(Arg499His) carriers. In vitro functional characterization shows that p.(Arg499His) PCSK9 variant causes a reduction in LDLr expression and LDL uptake. An intracellular activity for this variant is also shown when blocking the activity of secreted PCSK9 and by inhibiting endosomal acidification. Conclusions We demonstrated that p.(Arg499His) PCSK9 variant causes a direct intracellular degradation of LDLr therefore causing FH by reducing LDLr availability.
- Published
- 2019
10. OptiMo-LDLr: An Integrated In Silico Model with Enhanced Predictive Power for LDL Receptor Variants, Unraveling Hot Spot Pathogenic Residues.
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Larrea-Sebal A, Sasiain I, Jebari-Benslaiman S, Galicia-Garcia U, Uribe KB, Benito-Vicente A, Gracia-Rubio I, Bediaga-Bañeres H, Arrasate S, Cenarro A, Civeira F, González-Díaz H, and Martín C
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- Humans, Phenotype, Mutation, Receptors, LDL genetics, Receptors, LDL metabolism, Computer Simulation, Hyperlipoproteinemia Type II diagnosis, Hyperlipoproteinemia Type II genetics
- Abstract
Familial hypercholesterolemia (FH) is an inherited metabolic disease affecting cholesterol metabolism, with 90% of cases caused by mutations in the LDL receptor gene (LDLR), primarily missense mutations. This study aims to integrate six commonly used predictive software to create a new model for predicting LDLR mutation pathogenicity and mapping hot spot residues. Six predictive-software are selected: Polyphen-2, SIFT, MutationTaster, REVEL, VARITY, and MLb-LDLr. Software accuracy is tested with the characterized variants annotated in ClinVar and, by bioinformatic and machine learning techniques all models are integrated into a more accurate one. The resulting optimized model presents a specificity of 96.71% and a sensitivity of 98.36%. Hot spot residues with high potential of pathogenicity appear across all domains except for the signal peptide and the O-linked domain. In addition, translating this information into 3D structure of the LDLr highlights potentially pathogenic clusters within the different domains, which may be related to specific biological function. The results of this work provide a powerful tool to classify LDLR pathogenic variants. Moreover, an open-access guide user interface (OptiMo-LDLr) is provided to the scientific community. This study shows that combination of several predictive software results in a more accurate prediction to help clinicians in FH diagnosis., (© 2024 The Authors. Advanced Science published by Wiley‐VCH GmbH.)
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- 2024
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11. Predictive Modeling and Structure Analysis of Genetic Variants in Familial Hypercholesterolemia: Implications for Diagnosis and Protein Interaction Studies.
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Larrea-Sebal A, Jebari-Benslaiman S, Galicia-Garcia U, Jose-Urteaga AS, Uribe KB, Benito-Vicente A, and Martín C
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- Humans, Genetic Variation, Receptors, LDL genetics, Receptors, LDL metabolism, Mutation, Phenotype, Proprotein Convertase 9 genetics, Hyperlipoproteinemia Type II diagnosis, Hyperlipoproteinemia Type II genetics
- Abstract
Purpose of Review: Familial hypercholesterolemia (FH) is a hereditary condition characterized by elevated levels of low-density lipoprotein cholesterol (LDL-C), which increases the risk of cardiovascular disease if left untreated. This review aims to discuss the role of bioinformatics tools in evaluating the pathogenicity of missense variants associated with FH. Specifically, it highlights the use of predictive models based on protein sequence, structure, evolutionary conservation, and other relevant features in identifying genetic variants within LDLR, APOB, and PCSK9 genes that contribute to FH., Recent Findings: In recent years, various bioinformatics tools have emerged as valuable resources for analyzing missense variants in FH-related genes. Tools such as REVEL, Varity, and CADD use diverse computational approaches to predict the impact of genetic variants on protein function. These tools consider factors such as sequence conservation, structural alterations, and receptor binding to aid in interpreting the pathogenicity of identified missense variants. While these predictive models offer valuable insights, the accuracy of predictions can vary, especially for proteins with unique characteristics that might not be well represented in the databases used for training. This review emphasizes the significance of utilizing bioinformatics tools for assessing the pathogenicity of FH-associated missense variants. Despite their contributions, a definitive diagnosis of a genetic variant necessitates functional validation through in vitro characterization or cascade screening. This step ensures the precise identification of FH-related variants, leading to more accurate diagnoses. Integrating genetic data with reliable bioinformatics predictions and functional validation can enhance our understanding of the genetic basis of FH, enabling improved diagnosis, risk stratification, and personalized treatment for affected individuals. The comprehensive approach outlined in this review promises to advance the management of this inherited disorder, potentially leading to better health outcomes for those affected by FH., (© 2023. The Author(s).)
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- 2023
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12. Contribution of APOE Genetic Variants to Dyslipidemia.
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Bea AM, Larrea-Sebal A, Marco-Benedi V, Uribe KB, Galicia-Garcia U, Lamiquiz-Moneo I, Laclaustra M, Moreno-Franco B, Fernandez-Corredoira P, Olmos S, Civeira F, Martin C, and Cenarro A
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- Humans, Apolipoprotein E2 genetics, Apolipoprotein E3, Apolipoproteins E genetics, Apolipoproteins E metabolism, Receptors, LDL genetics, Receptors, LDL metabolism, Hypercholesterolemia genetics, Hyperlipidemias, Hyperlipoproteinemia Type III genetics
- Abstract
Background: apo (apolipoprotein) E has crucial role in lipid metabolism. The genetic variation in APOE gene is associated with monogenic disorders and contributes to polygenic hypercholesterolemia and to interindividual variability in cholesterol. APOE rare variants may be involved in the phenotype of genetic hyperlipidemias., Methods: Exon 4 of APOE were sequenced in all consecutive unrelated subjects with primary hyperlipidemia from a Lipid Unit (n=3667) and 822 random subjects from the Aragon Workers Health Study. Binding affinity of VLDL (very low-density lipoprotein) to LDL receptor of pathogenic predicted apoE variants was analyzed in vitro. Lipoprotein particle number, size, and composition were studied by nuclear magnetic resonance., Results: In addition to common polymorphisms giving rise to APOE2 and APOE4, 14 gene variants were found in exon 4 of APOE in 65 subjects. p.(Leu167del) in 8 patients with isolated hypercholesterolemia and in 8 patients with combined hyperlipidemia. Subjects with p.(Arg121Trp), p.(Gly145Asp), p.(Arg154Ser), p.(Arg163Cys), p.(Arg165Trp), and p.(Arg168His) variants met dysbetalipoproteinemia lipid criteria and were confirmed by nuclear magnetic resonance. VLDL affinity for the LDL receptor of p.(Arg163Cys) and p.(Arg165Trp) heterozygous carriers had intermedium affinity between APOE2/2 and APOE3/3. p.(Gly145Asp) and p.(Pro220Leu) variants had higher affinity than APOE3/3., Conclusions: APOE genetic variation contributes to the development of combined hyperlipidemia, usually dysbetalipoproteinemia, and familial hypercholesterolemia. The lipid phenotype in heterozygous for dysbetalipoproteinemia-associated mutations is milder than the homozygous APOE2/2-associated phenotype. Subjects with dysbetalipoproteinemia and absence of APOE2/2 are good candidates for the study of pathogenic variants in APOE . However, more investigation is required to elucidate the significance of rarer variants of apoE., Competing Interests: Disclosures None.
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- 2023
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13. Leu22_Leu23 Duplication at the Signal Peptide of PCSK9 Promotes Intracellular Degradation of LDLr and Autosomal Dominant Hypercholesterolemia.
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Benito-Vicente A, Uribe KB, Larrea-Sebal A, Palacios L, Cenarro A, Calle X, Galicia-Garcia U, Jebari-Benslaiman S, Sánchez-Hernández RM, Stef M, Lambert G, Civeira F, and Martín C
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- Cholesterol, LDL, Humans, Leucine, Protein Sorting Signals, Receptors, LDL genetics, Receptors, LDL metabolism, Hyperlipoproteinemia Type II diagnosis, Hyperlipoproteinemia Type II genetics, Proprotein Convertase 9 genetics, Proprotein Convertase 9 metabolism
- Abstract
Background: PCSK9 (Proprotein convertase subtilisin/kexin type 9) regulates LDL-C (low-density lipoprotein cholesterol) metabolism by targeting LDLr (LDL receptor) for lysosomal degradation. PCSK9 gain-of-function variants cause autosomal dominant hypercholesterolemia by reducing LDLr levels, the D374Y variant being the most severe, while loss-of-function variants are associated with low LDL-C levels. Gain-of-function and loss-of-function activities have also been attributed to variants occurring in the PCSK9 signal peptide. Among them, L11 is a very rare PCSK9 variant that seems to increase LDL-C values in a moderate way causing mild hypercholesterolemia., Methods: Using molecular biology and biophysics methodologies, activities of L8 and L11 variants, both located in the leucine repetition stretch of the signal peptide, have been extensively characterized in vitro., Results: L8 variant is not associated with increased LDLr activity, whereas L11 activity is increased by ≈20% compared with wt PCSK9. The results suggest that the L11 variant reduces LDLr levels intracellularly by a process resulting from impaired cleavage of the signal peptide. This would lead to less efficient LDLr transport to the cell membrane and promote LDLr intracellular degradation., Conclusions: Deletion of a leucine in the signal peptide in L8 variant does not affect PCSK9 activity, whereas the leucine duplication in the L11 variant enhances LDLr intracellular degradation. These findings highlight the importance of deep in vitro characterization of PCSK9 genetic variants to determine pathogenicity and improve clinical diagnosis and therapy of inherited familial hypercholesterolemia disease.
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- 2022
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14. Boosting Cholesterol Efflux from Foam Cells by Sequential Administration of rHDL to Deliver MicroRNA and to Remove Cholesterol in a Triple-Cell 2D Atherosclerosis Model.
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Jebari-Benslaiman S, Uribe KB, Benito-Vicente A, Galicia-Garcia U, Larrea-Sebal A, Santin I, Alloza I, Vandenbroeck K, Ostolaza H, and Martín C
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- Cholesterol, Foam Cells, Humans, Macrophages, Atherosclerosis drug therapy, MicroRNAs therapeutic use
- Abstract
Cardiovascular disease, the leading cause of mortality worldwide, is primarily caused by atherosclerosis, which is characterized by lipid and inflammatory cell accumulation in blood vessels and carotid intima thickening. Although disease management has improved significantly, new therapeutic strategies focused on accelerating atherosclerosis regression must be developed. Atherosclerosis models mimicking in vivo-like conditions provide essential information for research and new advances toward clinical application. New nanotechnology-based therapeutic opportunities have emerged with apoA-I nanoparticles (recombinant/reconstituted high-density lipoproteins, rHDL) as ideal carriers to deliver molecules and the discovery that microRNAs participate in atherosclerosis establishment and progression. Here, a therapeutic strategy to improve cholesterol efflux is developed based on a two-step administration of rHDL consisting of a first dose of antagomiR-33a-loaded rHDLs to induce adenosine triphosphate-binding cassette transporters A1 overexpression, followed by a second dose of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine rHDLs, which efficiently remove cholesterol from foam cells. A triple-cell 2D-atheroma plaque model reflecting the cellular complexity of atherosclerosis is used to improve efficiency of the nanoparticles in promoting cholesterol efflux. The results show that sequential administration of rHDL potentiates cholesterol efflux indicating that this approach may be used in vivo to more efficiently target atherosclerotic lesions and improve prognosis of the disease., (© 2022 Wiley-VCH GmbH.)
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- 2022
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15. A Systematic Approach to Assess the Activity and Classification of PCSK9 Variants.
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Uribe KB, Chemello K, Larrea-Sebal A, Benito-Vicente A, Galicia-Garcia U, Bourane S, Jaafar AK, Lambert G, and Martín C
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- Gain of Function Mutation, HEK293 Cells, Hep G2 Cells, Humans, Hyperlipoproteinemia Type II genetics, Hyperlipoproteinemia Type II metabolism, Mutagenesis, Site-Directed, Mutation, Proprotein Convertase 9 metabolism, Receptors, LDL metabolism, Proprotein Convertase 9 genetics
- Abstract
Background: Gain of function (GOF) mutations of PCSK9 cause autosomal dominant familial hypercholesterolemia as they reduce the abundance of LDL receptor (LDLR) more efficiently than wild-type PCSK9. In contrast, PCSK9 loss of function (LOF) variants are associated with a hypocholesterolemic phenotype. Dozens of PCSK9 variants have been reported, but most remain of unknown significance since their characterization has not been conducted., Objective: Our aim was to make the most comprehensive assessment of PCSK9 variants and to determine the simplest approach for the classification of these variants., Methods: The expression, maturation, secretion, and activity of nine well-established PCSK9 variants were assessed in transiently transfected HEK293 cells by Western blot and flow cytometry. Their extracellular activities were determined in HepG2 cells incubated with the purified recombinant PCSK9 variants. Their binding affinities toward the LDLR were determined by solid-phase immunoassay., Results: LDLR expression increased when cells were transfected with LOF variants and reduced when cells were transfected with GOF variants compared with wild-type PCSK9. Extracellular activities measurements yielded exactly similar results. GOF and LOF variants had increased, respectively reduced, affinities for the LDLR compared with wild-type PCSK9 with the exception of one GOF variant (R218S) that showed complete resistance to inactivation by furin. All variants were expressed at similar levels and underwent normal maturation and secretion patterns except for two LOF and two GOF mutants., Conclusions: We propose that transient transfections of HEK293 cells with a plasmid encoding a PCSK9 variant followed by LDLR expression assessment by flow cytometry is sufficient to reliably determine its GOF or LOF status. More refined experiments should only be used to determine the underlying mechanism(s) at hand.
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- 2021
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16. MLb-LDLr: A Machine Learning Model for Predicting the Pathogenicity of LDLr Missense Variants.
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Larrea-Sebal A, Benito-Vicente A, Fernandez-Higuero JA, Jebari-Benslaiman S, Galicia-Garcia U, Uribe KB, Cenarro A, Ostolaza H, Civeira F, Arrasate S, González-Díaz H, and Martín C
- Abstract
Untreated familial hypercholesterolemia (FH) leads to atherosclerosis and early cardiovascular disease. Mutations in the low-density lipoprotein receptor ( LDLr ) gene constitute the major cause of FH, and the high number of mutations already described in the LDLr makes necessary cascade screening or in vitro functional characterization to provide a definitive diagnosis. Implementation of high-predicting capacity software constitutes a valuable approach for assessing pathogenicity of LDLr variants to help in the early diagnosis and management of FH disease. This work provides a reliable machine learning model to accurately predict the pathogenicity of LDLr missense variants with specificity of 92.5% and sensitivity of 91.6%., Competing Interests: This study was supported by grants from the Basque Government (Cesar Martin, Grupos Consolidados IT-1264-19). Mr Larrea-Sebal was supported by a FPI grant from Gobierno Vasco (2019–2020). Dr Benito-Vicente was supported by Programa de especialización de Personal Investigador Doctor en la UPV/EHU (2019) 2019-2020. Dr Galicia-Garcia was supported by Fundación Biofísica Bizkaia. Ms Jebari-Benslaiman was supported by grant PIF (2017–2018), Gobierno Vasco. All other authors have reported that they have no relationships relevant to the contents of this paper to disclose., (© 2021 The Authors.)
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- 2021
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17. LDLR variants functional characterization: Contribution to variant classification.
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Alves AC, Azevedo S, Benito-Vicente A, Graça R, Galicia-Garcia U, Barros P, Jordan P, Martin C, and Bourbon M
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- Animals, CHO Cells, Cricetinae, Cricetulus, Humans, Mutation, Hyperlipoproteinemia Type II genetics, Receptors, LDL genetics
- Abstract
Background and Aims: Familial hypercholesterolaemia (FH) is an autosomal disorder of lipid metabolism presenting with increased cardiovascular risk. LDLR mutations are the cause of disease in 90% of the cases but functional studies have only been performed for about 15% of all LDLR variants. In the Portuguese Familial Hypercholesterolemia Study (PFHS), 142 unique LDLR alterations were identified and 44 (30%) lack functional characterization. The aim of the present work is to increase evidence for variant classification by performing functional characterization of 13 LDLR missense alterations found in Portugal and in 20 other countries., Methods: Different LDLR mutants were generated by site-directed mutagenesis and expressed in CHO-ldlA7 cells lacking endogenous expression of LDLR. To determine the effects of alterations on LDLR function, cell surface expression, binding and uptake of FITC-LDL were assessed by flow cytometry and Western blot., Results: Of the 13 variants studied 7 were shown to affect LDLR function - expression, binding or uptake, with rates lower than 60%: p.(Cys184Tyr), p.(Gly207_Ser213del); p.(His211Asp); p.(Asp221Tyr); p.(Glu288Lys); p.(Gly592Glu) and p.(Asp601Val)). The remaining 6 variants do not alter the LDLR function., Conclusions: These studies contributed to an update of these variants classification: from the 9 variants classified as variants of unknown significance, 7 have reached now a final classification and 3 variants have improved classification from likely pathogenic to pathogenic. In Portugal, an additional 55 patients received an FH definite diagnosis thanks to these studies. Since only likely pathogenic and pathogenic variants are clinically actionable, this work shows the importance of functional studies for variant classification., (Copyright © 2021 Elsevier B.V. All rights reserved.)
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- 2021
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18. Molecular mechanisms of lipotoxicity-induced pancreatic β-cell dysfunction.
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Benito-Vicente A, Jebari-Benslaiman S, Galicia-Garcia U, Larrea-Sebal A, Uribe KB, and Martin C
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- Animals, Glucose metabolism, Humans, Insulin Secretion drug effects, Insulin-Secreting Cells drug effects, Metabolome drug effects, Signal Transduction drug effects, Insulin-Secreting Cells pathology, Lipids toxicity
- Abstract
Type 2 diabetes (T2D), a heterogeneous disorder derived from metabolic dysfunctions, leads to a glucose overflow in the circulation due to both defective insulin secretion and peripheral insulin resistance. One of the critical risk factor for T2D is obesity, which represents a global epidemic that has nearly tripled since 1975. Obesity is characterized by chronically elevated free fatty acid (FFA) levels, which cause deleterious effects on glucose homeostasis referred to as lipotoxicity. Here, we review the physiological FFA roles onto glucose-stimulated insulin secretion (GSIS) and the pathological ones affecting many steps of the mechanisms and modulation of GSIS. We also describe in vitro and in vivo experimental evidences addressing lipotoxicity in β-cells and the role of saturation and chain length of FFA on the potency of GSIS stimulation. The molecular mechanisms underpinning lipotoxic-β-cell dysfunction are also reviewed. Among them, endoplasmic reticulum stress, oxidative stress and mitochondrial dysfunction, inflammation, impaired autophagy and β-cell dedifferentiation. Finally therapeutic strategies for the β-cells dysfunctions such as the use of metformin, glucagon-like peptide 1, thiazolidinediones, anti-inflammatory drugs, chemical chaperones and weight are discussed., (Copyright © 2021 Elsevier Inc. All rights reserved.)
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- 2021
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19. Cholesterol Efflux Efficiency of Reconstituted HDL Is Affected by Nanoparticle Lipid Composition.
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Jebari-Benslaiman S, Uribe KB, Benito-Vicente A, Galicia-Garcia U, Larrea-Sebal A, Alloza I, Vandenbroeck K, Ostolaza H, and Martín C
- Abstract
Cardiovascular disease (CVD), the leading cause of mortality worldwide is primarily caused by atherosclerosis, which is promoted by the accumulation of low-density lipoproteins into the intima of large arteries. Multiple nanoparticles mimicking natural HDL (rHDL) have been designed to remove cholesterol excess in CVD therapy. The goal of this investigation was to assess the cholesterol efflux efficiency of rHDLs with different lipid compositions, mimicking different maturation stages of high-density lipoproteins (HDLs) occurring in vivo., Methods: the cholesterol efflux activity of soybean PC (Soy-PC), 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), DPPC:Chol:1-palmitoyl-2-hydroxy-sn-glycero-3-phosphocholine (LysoPC) and DPPC:18:2 cholesteryl ester (CE):LysoPC rHDLs was determined in several cell models to investigate the contribution of lipid composition to the effectiveness of cholesterol removal., Results: DPPC rHDLs are the most efficient particles, inducing cholesterol efflux in all cellular models and in all conditions the effect was potentiated when the ABCA1 transporter was upregulated., Conclusions: DPPC rHDLs, which resemble nascent HDL, are the most effective particles in inducing cholesterol efflux due to the higher physical binding affinity of cholesterol to the saturated long-chain-length phospholipids and the favored cholesterol transfer from a highly positively curved bilayer, to an accepting planar bilayer such as DPPC rHDLs. The physicochemical characteristics of rHDLs should be taken into consideration to design more efficient nanoparticles to promote cholesterol efflux.
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- 2020
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20. Pathophysiology of Type 2 Diabetes Mellitus.
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Galicia-Garcia U, Benito-Vicente A, Jebari S, Larrea-Sebal A, Siddiqi H, Uribe KB, Ostolaza H, and Martín C
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- Animals, Humans, Blood Glucose metabolism, Diabetes Mellitus, Type 2 physiopathology, Homeostasis, Insulin Secretion
- Abstract
Type 2 Diabetes Mellitus (T2DM), one of the most common metabolic disorders, is caused by a combination of two primary factors: defective insulin secretion by pancreatic β-cells and the inability of insulin-sensitive tissues to respond appropriately to insulin. Because insulin release and activity are essential processes for glucose homeostasis, the molecular mechanisms involved in the synthesis and release of insulin, as well as in its detection are tightly regulated. Defects in any of the mechanisms involved in these processes can lead to a metabolic imbalance responsible for the development of the disease. This review analyzes the key aspects of T2DM, as well as the molecular mechanisms and pathways implicated in insulin metabolism leading to T2DM and insulin resistance. For that purpose, we summarize the data gathered up until now, focusing especially on insulin synthesis, insulin release, insulin sensing and on the downstream effects on individual insulin-sensitive organs. The review also covers the pathological conditions perpetuating T2DM such as nutritional factors, physical activity, gut dysbiosis and metabolic memory. Additionally, because T2DM is associated with accelerated atherosclerosis development, we review here some of the molecular mechanisms that link T2DM and insulin resistance (IR) as well as cardiovascular risk as one of the most important complications in T2DM.
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- 2020
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21. Statin Treatment-Induced Development of Type 2 Diabetes: From Clinical Evidence to Mechanistic Insights.
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Galicia-Garcia U, Jebari S, Larrea-Sebal A, Uribe KB, Siddiqi H, Ostolaza H, Benito-Vicente A, and Martín C
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- Adipocytes metabolism, Cardiovascular Diseases metabolism, Glucose Transporter Type 4 metabolism, Humans, Hydroxymethylglutaryl-CoA Reductase Inhibitors therapeutic use, Hyperinsulinism metabolism, Insulin metabolism, Insulin Resistance physiology, Insulin Secretion drug effects, Insulin-Secreting Cells metabolism, Risk Factors, Diabetes Mellitus, Type 2 etiology, Diabetes Mellitus, Type 2 metabolism, Hydroxymethylglutaryl-CoA Reductase Inhibitors adverse effects
- Abstract
Statins are the gold-standard treatment for the prevention of primary and secondary cardiovascular disease, which is the leading cause of mortality worldwide. Despite the safety and relative tolerability of statins, observational studies, clinical trials and meta-analyses indicate an increased risk of developing new-onset type 2 diabetes mellitus (T2DM) after long-term statin treatment. It has been shown that statins can impair insulin sensitivity and secretion by pancreatic β-cells and increase insulin resistance in peripheral tissues. The mechanisms involved in these processes include, among others, impaired Ca
2+ signaling in pancreatic β-cells, down-regulation of GLUT-4 in adipocytes and compromised insulin signaling. In addition, it has also been described that statins' impact on epigenetics may also contribute to statin-induced T2DM via differential expression of microRNAs. This review focuses on the evidence and mechanisms by which statin therapy is associated with the development of T2DM. This review describes the multifactorial combination of effects that most likely contributes to the diabetogenic effects of statins. Clinically, these findings should encourage clinicians to consider diabetes monitoring in patients receiving statin therapy in order to ensure early diagnosis and appropriate management.- Published
- 2020
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22. Mutation type classification and pathogenicity assignment of sixteen missense variants located in the EGF-precursor homology domain of the LDLR.
- Author
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Galicia-Garcia U, Benito-Vicente A, Uribe KB, Jebari S, Larrea-Sebal A, Alonso-Estrada R, Aguilo-Arce J, Ostolaza H, Palacios L, and Martin C
- Subjects
- Animals, CHO Cells, Cricetulus, Epidermal Growth Factor genetics, Humans, Lipoproteins, LDL metabolism, Phenotype, Polymorphism, Genetic, Protein Domains genetics, Receptors, LDL metabolism, Hyperlipoproteinemia Type II genetics, Mutation, Missense genetics, Receptors, LDL genetics
- Abstract
The primary genetic cause of familial hypercholesterolemia (FH) is related to mutations in the LDLR gene encoding the Low-density Lipoprotein Receptor. LDLR structure is organized in 5 different domains, including an EGF-precursor homology domain that plays a pivotal role in lipoprotein release and receptor recycling. Mutations in this domain constitute 51.7% of the total missense variants described in LDLR. The aim of the present work was to analyse how clinically significant variants in the EGF-precursor homology domain impact LDLR. The activity of sixteen LDLR variants was functionally characterized by determining LDLR expression by Western blot and LDLR expression, LDL binding capacity and uptake, and LDLR recycling activity by flow cytometry in transfected CHO-ldlA7 cells. Of the analysed variants, we found six non-pathogenic LDLR variants and ten pathogenic variants distributed as follow: three class 3 variants; four class 2 variants; and three class 5 variants. These results can be incorporated into clinical management of patients by helping guide the appropriate level of treatment intensity depending on the extent of loss of LDLR activity. This data can also contribute to cascade-screening for pathogenic FH variants.
- Published
- 2020
- Full Text
- View/download PDF
23. The Arg499His gain-of-function mutation in the C-terminal domain of PCSK9.
- Author
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Sánchez-Hernández RM, Di Taranto MD, Benito-Vicente A, Uribe KB, Lamiquiz-Moneo I, Larrea-Sebal A, Jebari S, Galicia-Garcia U, Nóvoa FJ, Boronat M, Wägner AM, Civeira F, Martín C, and Fortunato G
- Subjects
- Cell Membrane metabolism, Child, Culture Media, Family Health, Female, HEK293 Cells, Hep G2 Cells, Heterozygote, Humans, Italy, Middle Aged, Pedigree, Protein Domains, Receptors, LDL metabolism, Spain, Arginine chemistry, Gain of Function Mutation, Histidine chemistry, Proprotein Convertase 9 genetics
- Abstract
Background and Aims: Familial hypercholesterolemia (FH) is a monogenic disease characterized by high levels of low-density lipoprotein cholesterol and premature atherosclerotic cardiovascular disease. FH is caused by loss of function mutations in genes encoding LDL receptor (LDLR), and Apolipoprotein B (APOB) or gain of function (GOF) mutations in proprotein convertase subtilisin/kexin type 9 (PCSK9). In this study, we identified a novel variant in PCSK9, p.(Arg499His), located in the C-terminal domain, in two unrelated FH patients from Spain and Italy., Methods: We studied familial segregation and determined variant activity in vitro., Results: We determined PCSK9 expression, secretion and activity of the variant in transfected HEK293 cells; extracellular activity of the recombinant p.(Arg499His) PCSK9 variant in HEK 293 and HepG2 cells; PCSK9 affinity to the LDL receptor at neutral and acidic pH; the mechanism of action of the p.(Arg499His) PCSK9 variant by co-transfection with a soluble construct of the LDL receptor and by determining total PCSK9 intracellular accumulation when endosomal acidification is impaired and when an excess of soluble LDLr is present in the culture medium. Our results show high LDL-C concentrations and FH phenotype in p.(Arg499His) carriers. In vitro functional characterization shows that p.(Arg499His) PCSK9 variant causes a reduction in LDLr expression and LDL uptake. An intracellular activity for this variant is also shown when blocking the activity of secreted PCSK9 and by inhibiting endosomal acidification., Conclusions: We demonstrated that p.(Arg499His) PCSK9 variant causes a direct intracellular degradation of LDLr therefore causing FH by reducing LDLr availability., (Copyright © 2019 Elsevier B.V. All rights reserved.)
- Published
- 2019
- Full Text
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24. Familial Hypercholesterolemia: The Most Frequent Cholesterol Metabolism Disorder Caused Disease.
- Author
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Benito-Vicente A, Uribe KB, Jebari S, Galicia-Garcia U, Ostolaza H, and Martin C
- Subjects
- Animals, Biological Transport, Dietary Supplements, Genetic Testing, Humans, Hyperlipoproteinemia Type II diagnosis, Hyperlipoproteinemia Type II genetics, Hyperlipoproteinemia Type II therapy, Cholesterol metabolism, Hyperlipoproteinemia Type II pathology
- Abstract
Cholesterol is an essential component of cell barrier formation and signaling transduction involved in many essential physiologic processes. For this reason, cholesterol metabolism must be tightly controlled. Cell cholesterol is mainly acquired from two sources: Dietary cholesterol, which is absorbed in the intestine and, intracellularly synthesized cholesterol that is mainly synthesized in the liver. Once acquired, both are delivered to peripheral tissues in a lipoprotein dependent mechanism. Malfunctioning of cholesterol metabolism is caused by multiple hereditary diseases, including Familial Hypercholesterolemia, Sitosterolemia Type C and Niemann-Pick Type C1. Of these, familial hypercholesterolemia (FH) is a common inherited autosomal co-dominant disorder characterized by high plasma cholesterol levels. Its frequency is estimated to be 1:200 and, if untreated, increases the risk of premature cardiovascular disease. This review aims to summarize the current knowledge on cholesterol metabolism and the relation of FH to cholesterol homeostasis with special focus on the genetics, diagnosis and treatment.
- Published
- 2018
- Full Text
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25. Validation of LDLr Activity as a Tool to Improve Genetic Diagnosis of Familial Hypercholesterolemia: A Retrospective on Functional Characterization of LDLr Variants.
- Author
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Benito-Vicente A, Uribe KB, Jebari S, Galicia-Garcia U, Ostolaza H, and Martin C
- Subjects
- DNA Mutational Analysis, Genetic Variation, Humans, Mutation, Phenotype, Retrospective Studies, Genetic Testing, Hyperlipoproteinemia Type II diagnosis, Hyperlipoproteinemia Type II genetics, Receptors, LDL genetics
- Abstract
Familial hypercholesterolemia (FH) is an autosomal dominant disorder characterized by high blood-cholesterol levels mostly caused by mutations in the low-density lipoprotein receptor (LDLr). With a prevalence as high as 1/200 in some populations, genetic screening for pathogenic LDLr mutations is a cost-effective approach in families classified as 'definite' or 'probable' FH and can help to early diagnosis. However, with over 2000 LDLr variants identified, distinguishing pathogenic mutations from benign mutations is a long-standing challenge in the field. In 1998, the World Health Organization (WHO) highlighted the importance of improving the diagnosis and prognosis of FH patients thus, identifying LDLr pathogenic variants is a longstanding challenge to provide an accurate genetic diagnosis and personalized treatments. In recent years, accessible methodologies have been developed to assess LDLr activity in vitro, providing experimental reproducibility between laboratories all over the world that ensures rigorous analysis of all functional studies. In this review we present a broad spectrum of functionally characterized missense LDLr variants identified in patients with FH, which is mandatory for a definite diagnosis of FH., Competing Interests: The authors declare no conflict of interest.
- Published
- 2018
- Full Text
- View/download PDF
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