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1. Management of the axilla in breast cancer patients: critical review, regional modified Delphi consensus and implementation in the Tuscan breast network

Author

Ghilli, Matteo, Becherini, Carlotta, Meattini, Icro, Angiolini, Catia, Bengala, Carmelo, Marconi, Aroldo, Galli, Lorenzo, Angiolucci, Giovanni, Coltelli, Luigi, Borghesi, Simona, Lastrucci, Luciana, Manca, Gianpiero, Bianchi, Simonetta, Doria, Morena, Casella, Donato, Marotti, Lorenza, Amunni, Gianni, and Roncella, Manuela

Published
2024
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2. Oncoplastic and reconstructive surgery in SENONETWORK Italian breast centers: lights and shadows

Author

Abbonante, Francesco, Lamanna, Ginevra, Lombardi, Augusto, Vischi, Silvio, Orzalesi, Lorenzo, Angiolucci, Giovanni, Bozzo, Samantha, Pizzorno, Laura, Ballardini, Bettina, Barbero, Maggiorino, Barellini, Leonardo, Battaglia, Claudio, Reggiani, Luisa, Santi, Caterina, Biglio, Nicoletta, Bortul, Marina, Burelli, Paolo, Busani, Massimo, Cabula, Roberta, Cagossi, Katia, Fontanarosa, Vito Maria, Catalano, Francesca, Cedolini, Carla, Ciuffreda, Luigi, Corsi, Fabio, Custodero, Olindo, Mori, Stefano, De Vita, Roy, Defilippi, Loredana, Marcuzzi, Samantha, Drago, Stefano, Battista, Giovanni, Burgoa, Loredana, Cristofolini, Paolo, Romanucci, Giovanna, Loreti, Andrea, Prosperi, Valerio, Carcoforo, Paolo, Franzini, Patrizia Fulvia, Frittelli, Patrizia, Perniciaro, Giuseppe, Generali, Daniele, Giordano, Monica, Riccardo, Giovanazzi, Grossi, Simona, Huscher, Alessandra, La Torre, Giuseppe, Lolli, Gianfranco, Magni, Carla, Mancini, Stefano, Galli, Lorenzo, Di Leone, Alba, Massarut, Samuele, Massocco, Alberto, Cramarossa, Monica, Meneghini, Graziano, Fabiocchi, Luca, Miglietta, Anna Maria, Millo, Francesco, Ciabattoni, Antonella, Pellini, Francesca, Moschetta, Marco, Musolino, Antonino, Palli, Dante, Pagura, Giulia, Pieraccini, Mariagrazia, Marenco, Davide, Polato, Romano, Renne, Maria, Ressa, Cosmo Maurizio, Ricci, Fabio, Ridolfo, Raffaella, Rovera, Francesca Angela, Barberini, Francesco, Vinciguerra, Marina, Furci, Marco, Sciamannini, Maria, Gianquinto, Daniela, Petrucci, Silvia, Della Valle, Angelica, Stancampiano, Pietra, Lippi, Andrea, Tazzioli, Giovanni, Lombardi, Davide, Trunfio, Martino, Valieri, Luca, Vecchio, Carlo, Veronesi, Paolo, Grilz, Gretha, Ghilli, Matteo, Lisa, Andrea Vittorio Emanuele, Salgarello, Marzia, Papa, Giovanni, Rietjens, Mario, Folli, Secondo, Curcio, Annalisa, Ferrari, Guglielmo, Caruso, Francesco, Altomare, Vittorio, Friedman, Daniele, De Santis, Maria Carmen, De Rose, Fiorenza, Meduri, Bruno, De Felice, Francesca, Marino, Lorenza, Cucciarelli, Francesca, Montemezzi, Stefania, Panizza, Pietro, Belli, Paolo, Caumo, Francesca, Vinci, Valeriano, De Santis, Giorgio, Klinger, Marco, and Roncella, Manuela

Published
2024
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3. Analysis of XFEL serial diffraction data from individual crystalline fibrils

Author

Wojtas, David H, Ayyer, Kartik, Liang, Mengning, Mossou, Estelle, Romoli, Filippo, Seuring, Carolin, Beyerlein, Kenneth R, Bean, Richard J, Morgan, Andrew J, Oberthuer, Dominik, Fleckenstein, Holger, Heymann, Michael, Gati, Cornelius, Yefanov, Oleksandr, Barthelmess, Miriam, Ornithopoulou, Eirini, Galli, Lorenzo, Xavier, P Lourdu, Ling, Wai Li, Frank, Matthias, Yoon, Chun Hong, White, Thomas A, Bajt, Saša, Mitraki, Anna, Boutet, Sebastien, Aquila, Andrew, Barty, Anton, Forsyth, V Trevor, Chapman, Henry N, and Millane, Rick P

Subjects
Inorganic Chemistry, Chemical Sciences, Physical Sciences, Brain Disorders, Neurodegenerative, serial crystallography, coherent X-ray diffractive imaging, single particles, molecular orientation determination, crystalline fibrils, amyloid, Atomic, Molecular, Nuclear, Particle and Plasma Physics, Condensed Matter Physics, Physical Chemistry (incl. Structural), Physical chemistry, Condensed matter physics
Abstract

Serial diffraction data collected at the Linac Coherent Light Source from crystalline amyloid fibrils delivered in a liquid jet show that the fibrils are well oriented in the jet. At low fibril concentrations, diffraction patterns are recorded from single fibrils; these patterns are weak and contain only a few reflections. Methods are developed for determining the orientation of patterns in reciprocal space and merging them in three dimensions. This allows the individual structure amplitudes to be calculated, thus overcoming the limitations of orientation and cylindrical averaging in conventional fibre diffraction analysis. The advantages of this technique should allow structural studies of fibrous systems in biology that are inaccessible using existing techniques.

Published
2017

4. XFEL and NMR Structures of Francisella Lipoprotein Reveal Conformational Space of Drug Target against Tularemia

Author

Zook, James, Shekhar, Mrinal, Hansen, Debra, Conrad, Chelsie, Grant, Thomas, Gupta, Chitrak, White, Thomas, Barty, Anton, Basu, Shibom, Zhao, Yun, Zatsepin, Nadia, Ishchenko, Andrii, Batyuk, Alex, Gati, Cornelius, Li, Chufeng, Galli, Lorenzo, Coe, Jesse, Hunter, Mark, Liang, Meng, Weierstall, Uwe, Nelson, Garret, James, Daniel, Stauch, Benjamin, Craciunescu, Felicia, Thifault, Darren, Liu, Wei, Cherezov, Vadim, Singharoy, Abhishek, and Fromme, Petra

Published
2020
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5. Oncoplastic and reconstructive surgery in SENONETWORK Italian breast centers: lights and shadows

Author

Ghilli, Matteo, primary, Lisa, Andrea Vittorio Emanuele, additional, Salgarello, Marzia, additional, Papa, Giovanni, additional, Rietjens, Mario, additional, Folli, Secondo, additional, Curcio, Annalisa, additional, Ferrari, Guglielmo, additional, Caruso, Francesco, additional, Altomare, Vittorio, additional, Friedman, Daniele, additional, De Santis, Maria Carmen, additional, De Rose, Fiorenza, additional, Meduri, Bruno, additional, De Felice, Francesca, additional, Marino, Lorenza, additional, Cucciarelli, Francesca, additional, Montemezzi, Stefania, additional, Panizza, Pietro, additional, Belli, Paolo, additional, Caumo, Francesca, additional, Vinci, Valeriano, additional, De Santis, Giorgio, additional, Klinger, Marco, additional, Roncella, Manuela, additional, Abbonante, Francesco, additional, Lamanna, Ginevra, additional, Lombardi, Augusto, additional, Vischi, Silvio, additional, Orzalesi, Lorenzo, additional, Angiolucci, Giovanni, additional, Bozzo, Samantha, additional, Pizzorno, Laura, additional, Ballardini, Bettina, additional, Barbero, Maggiorino, additional, Barellini, Leonardo, additional, Battaglia, Claudio, additional, Reggiani, Luisa, additional, Santi, Caterina, additional, Biglio, Nicoletta, additional, Bortul, Marina, additional, Burelli, Paolo, additional, Busani, Massimo, additional, Cabula, Roberta, additional, Cagossi, Katia, additional, Fontanarosa, Vito Maria, additional, Catalano, Francesca, additional, Cedolini, Carla, additional, Ciuffreda, Luigi, additional, Corsi, Fabio, additional, Custodero, Olindo, additional, Mori, Stefano, additional, De Vita, Roy, additional, Defilippi, Loredana, additional, Marcuzzi, Samantha, additional, Drago, Stefano, additional, Battista, Giovanni, additional, Burgoa, Loredana, additional, Cristofolini, Paolo, additional, Romanucci, Giovanna, additional, Loreti, Andrea, additional, Prosperi, Valerio, additional, Carcoforo, Paolo, additional, Franzini, Patrizia Fulvia, additional, Frittelli, Patrizia, additional, Perniciaro, Giuseppe, additional, Generali, Daniele, additional, Giordano, Monica, additional, Riccardo, Giovanazzi, additional, Grossi, Simona, additional, Huscher, Alessandra, additional, La Torre, Giuseppe, additional, Lolli, Gianfranco, additional, Magni, Carla, additional, Mancini, Stefano, additional, Galli, Lorenzo, additional, Di Leone, Alba, additional, Massarut, Samuele, additional, Massocco, Alberto, additional, Cramarossa, Monica, additional, Meneghini, Graziano, additional, Fabiocchi, Luca, additional, Miglietta, Anna Maria, additional, Millo, Francesco, additional, Ciabattoni, Antonella, additional, Pellini, Francesca, additional, Moschetta, Marco, additional, Musolino, Antonino, additional, Palli, Dante, additional, Pagura, Giulia, additional, Pieraccini, Mariagrazia, additional, Marenco, Davide, additional, Polato, Romano, additional, Renne, Maria, additional, Ressa, Cosmo Maurizio, additional, Ricci, Fabio, additional, Ridolfo, Raffaella, additional, Rovera, Francesca Angela, additional, Barberini, Francesco, additional, Vinciguerra, Marina, additional, Furci, Marco, additional, Sciamannini, Maria, additional, Gianquinto, Daniela, additional, Petrucci, Silvia, additional, Della Valle, Angelica, additional, Stancampiano, Pietra, additional, Lippi, Andrea, additional, Tazzioli, Giovanni, additional, Lombardi, Davide, additional, Trunfio, Martino, additional, Valieri, Luca, additional, Vecchio, Carlo, additional, Veronesi, Paolo, additional, and Grilz, Gretha, additional

Published
2024
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6. Metabolic labelling of a subpopulation of small extracellular vesicles using a fluorescent palmitic acid analogue

Author

Barreca, Valeria, primary, Boussadia, Zaira, additional, Polignano, Deborah, additional, Galli, Lorenzo, additional, Tirelli, Valentina, additional, Sanchez, Massimo, additional, Falchi, Mario, additional, Bertuccini, Lucia, additional, Iosi, Francesca, additional, Tatti, Massimo, additional, Sargiacomo, Massimo, additional, and Fiani, Maria Luisa, additional

Published
2023
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7. The clinical impact of pectoral nerve block in an ‘enhanced recovery after surgery’ program in breast surgery

Author

Conti, Duccio, primary, Valoriani, Juri, additional, Ballo, Piercarlo, additional, Pazzi, Maddalena, additional, Gianesello, Lara, additional, Mengoni, Veronica, additional, Criscenti, Valentina, additional, Gemmi, Eleonora, additional, Stera, Caterina, additional, Zoppi, Federica, additional, Galli, Lorenzo, additional, and Pavoni, Vittorio, additional

Published
2023
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8. Atomic structure of granulin determined from native nanocrystalline granulovirus using an X-ray free-electron laser

Author

Gati, Cornelius, Oberthuer, Dominik, Yefanov, Oleksandr, Bunker, Richard D., Stellato, Francesco, Chiu, Elaine, Yeh, Shin-Mei, Aquila, Andrew, Basu, Shibom, Bean, Richard, Beyerlein, Kenneth R., Botha, Sabine, Boutet, Sébastien, DePonte, Daniel P., Doak, R. Bruce, Fromme, Raimund, Galli, Lorenzo, Grotjohann, Ingo, James, Daniel R., Kupitz, Christopher, Lomb, Lukas, Messerschmidt, Marc, Nass, Karol, Rendek, Kimberly, Shoeman, Robert L., Wang, Dingjie, Weierstall, Uwe, White, Thomas A., Williams, Garth J., Zatsepin, Nadia A., Fromme, Petra, Spence, John C. H., Goldie, Kenneth N., Jehle, Johannes A., Metcalf, Peter, Barty, Anton, and Chapman, Henry N.

Published
2017

9. Impact of hormonal status on outcome of ductal carcinoma in situ treated with breast-conserving surgery plus radiotherapy: Long-term experience from two large-institutional series

Author

Meattini, Icro, Saieva, Calogero, Bastiani, Paolo, Martella, Francesca, Francolini, Giulio, lo Russo, Monica, Paoletti, Lisa, Doria, Morena, Desideri, Isacco, Terziani, Francesca, De Luca Cardillo, Carla, Bendinelli, Benedetta, Ciabatti, Cinzia, Muntoni, Cristina, Tinacci, Galliano, Nori, Jacopo, Smith, Herd, Brancato, Beniamino, Galli, Lorenzo, Sanchez, Luis Jose, Casella, Donato, Bernini, Marco, Orzalesi, Lorenzo, Carta, Giulio Alberto, Bianchi, Simonetta, Rossi, Francesca, and Livi, Lorenzo

Published
2017
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10. Natively Inhibited Trypanosoma brucei Cathepsin B Structure Determined by Using an X-ray Laser

Author

Redecke, Lars, Nass, Karol, Deponte, Daniel P., White, Thomas A., Rehders, Dirk, Barty, Anton, Stellato, Francesco, Liang, Mengning, Barends, Thomas R.M., Boutet, Sébastien, Williams, Garth J., Messerschmidt, Marc, Seibert, M. Marvin, Aquila, Andrew, Arnlund, David, Bajt, Sasa, Barth, Torsten, Bogan, Michael J., Caleman, Carl, Chao, Tzu-Chiao, Doak, R. Bruce, Fleckenstein, Holger, Frank, Matthias, Fromme, Raimund, Galli, Lorenzo, Grotjohann, Ingo, Hunter, Mark S., Johansson, Linda C., Kassemeyer, Stephan, Katona, Gergely, Kirian, Richard A., Koopmann, Rudolf, Kupitz, Chris, Lomb, Lukas, Martin, Andrew V., Mogk, Stefan, Neutze, Richard, Shoeman, Robert L., Steinbrener, Jan, Timneanu, Nicusor, Wang, Dingjie, Weierstall, Uwe, Zatsepin, Nadia A., Spence, John C. H., Fromme, Petra, Schlichting, Ilme, Duszenko, Michael, Betzel, Christian, and Chapman, Henry N.

Published
2013
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11. Serial time-resolved crystallography of photosystem II using a femtosecond X-ray laser

Author

Kupitz, Christopher, Basu, Shibom, Grotjohann, Ingo, Fromme, Raimund, Zatsepin, Nadia A., Rendek, Kimberly N., Hunter, Mark S., Shoeman, Robert L., White, Thomas A., Wang, Dingjie, James, Daniel, Yang, Jay-How, Cobb, Danielle E., Reeder, Brenda, Sierra, Raymond G., Liu, Haiguang, Barty, Anton, Aquila, Andrew L., Deponte, Daniel, Kirian, Richard A., Bari, Sadia, Bergkamp, Jesse J., Beyerlein, Kenneth R., Bogan, Michael J., Caleman, Carl, Chao, Tzu-Chiao, Conrad, Chelsie E., Davis, Katherine M., Fleckenstein, Holger, Galli, Lorenzo, Hau-Riege, Stefan P., Kassemeyer, Stephan, Laksmono, Hartawan, Liang, Mengning, Lomb, Lukas, Marchesini, Stefano, Martin, Andrew V., Messerschmidt, Marc, Milathianaki, Despina, Nass, Karol, Ros, Alexandra, Roy-Chowdhury, Shatabdi, Schmidt, Kevin, Seibert, Marvin, Steinbrener, Jan, Stellato, Francesco, Yan, Lifen, Yoon, Chunhong, Moore, Thomas A., Moore, Ana L., Pushkar, Yulia, Williams, Garth J., Boutet, Sébastien, Doak, R. Bruce, Weierstall, Uwe, Frank, Matthias, Chapman, Henry N., Spence, John C. H., and Fromme, Petra

Published
2014
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12. Macromolecular diffractive imaging using imperfect crystals

Author

Ayyer, Kartik, Yefanov, Oleksandr M., Oberthür, Dominik, Roy-Chowdhury, Shatabdi, Galli, Lorenzo, Mariani, Valerio, Basu, Shibom, Coe, Jesse, Conrad, Chelsie E., Fromme, Raimund, Schaffer, Alexander, Dörner, Katerina, James, Daniel, Kupitz, Christopher, Metz, Markus, Nelson, Garrett, Xavier, Paulraj Lourdu, Beyerlein, Kenneth R., Schmidt, Marius, Sarrou, Iosifina, Spence, John C. H., Weierstall, Uwe, White, Thomas A., Yang, Jay-How, Zhao, Yun, Liang, Mengning, Aquila, Andrew, Hunter, Mark S., Robinson, Joseph S., Koglin, Jason E., Boutet, Sébastien, Fromme, Petra, Barty, Anton, and Chapman, Henry N.

Published
2016
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13. Recent developments in $Cryst$FEL

Author

White, Thomas, Mariani, Valerio, Brehm, Wolfgang, Yefanov, Oleksandr, Barty, Anton, Beyerlein, Kenneth, Chervinskii, Fedor, Galli, Lorenzo, Gati, Cornelius, Nakane, Takanori, Tolstikova, Alexandra, Yamashita, Keitaro, Yoon, Chun Hong, Diederichs, Kay, and Chapman, Henry N.

Subjects
ddc:540
Abstract

Journal of applied crystallography 49(2), 680 - 689(2016). doi:10.1107/S1600576716004751, $Cryst$FEL is a suite of programs for processing data from `serial crystallography' experiments, which are usually performed using X-ray free-electron lasers (FELs) but also increasingly with other X-ray sources. The $Cryst$FEL software suite has been under development since 2009, just before the first hard FEL experiments were performed, and has been significantly updated and improved since then. This article describes the most important improvements which have been made to $Cryst$FEL since the first release version. These changes include the addition of new programs to the suite, the ability to resolve `indexing ambiguities' and several ways to improve the quality of the integrated data by more accurately modelling the underlying diffraction physics., Published by Munksgaard, Copenhagen

Published
2016
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14. Phasing using high intensity free-electron laser radiation

Author

Galli, Lorenzo

Abstract

X-ray free-electron lasers (XFELs) provide extremely bright X-ray pulses of femtosecond duration, that promise to revolutionize structural biology, as they can be used to collect diffraction data from micrometer-sized crystals while outrunning ra- diation damage. The high fluence of the XFEL pulses induces severe electronic radiation damage to the sample, and especially the heavy atoms are strongly ion- ized by the X-ray radiation. The aim of this thesis is to test if it may be possible to use this specific radiation damage effect as a new approach to phasing.By simulating serial femtosecond crystallography experiments at different X-ray fluence conditions, I describe that it is possible to use a Radiation damage-Induced Phasing scheme to retrieve the coordinates of the heavy atoms, and to correctly phase the model structure. Experimental data showed an effective reduction of the scattering power of a heavy atom inside a chemically modified protein, and of the sulfurs in a native protein. From the analysis of these experimental data, quantitative methods have been developed to retrieve information about the effective ionization of the damaged atomic species. The same analysis demonstrated that statistical methods can be used to sort the collected diffraction patterns, according to photon flux impinging on the sample. The knowledge of the real experimental conditions is critical for the success of high intensity phasing technique.

Published
2015

15. Native phasing of x-ray free-electron laser data for a G protein–coupled receptor

Author

Batyuk, Alexander, primary, Galli, Lorenzo, additional, Ishchenko, Andrii, additional, Han, Gye Won, additional, Gati, Cornelius, additional, Popov, Petr A., additional, Lee, Ming-Yue, additional, Stauch, Benjamin, additional, White, Thomas A., additional, Barty, Anton, additional, Aquila, Andrew, additional, Hunter, Mark S., additional, Liang, Mengning, additional, Boutet, Sébastien, additional, Pu, Mengchen, additional, Liu, Zhi-jie, additional, Nelson, Garrett, additional, James, Daniel, additional, Li, Chufeng, additional, Zhao, Yun, additional, Spence, John C. H., additional, Liu, Wei, additional, Fromme, Petra, additional, Katritch, Vsevolod, additional, Weierstall, Uwe, additional, Stevens, Raymond C., additional, and Cherezov, Vadim, additional

Published
2016
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16. Recent developments in CrystFEL

Author

White, Thomas A., primary, Mariani, Valerio, additional, Brehm, Wolfgang, additional, Yefanov, Oleksandr, additional, Barty, Anton, additional, Beyerlein, Kenneth R., additional, Chervinskii, Fedor, additional, Galli, Lorenzo, additional, Gati, Cornelius, additional, Nakane, Takanori, additional, Tolstikova, Alexandra, additional, Yamashita, Keitaro, additional, Yoon, Chun Hong, additional, Diederichs, Kay, additional, and Chapman, Henry N., additional

Published
2016
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17. Distribution of invasive meningococcal B disease in Italian pediatric population: implications for vaccination timing

Author

Azzari, Chiara, Canessa, Clementina, Lippi, Francesca, Moriondo, Maria, Indolfi, Giuseppe, Nieddu, Francesco, Martini, Marco, de Martino, Maurizio, Castiglia, Paolo, Baldo, Vincenzo, Resti, Massimo, Agostiniani, R., Allievi, P., Allù, G., Amigoni, A., Bartolini, E., Bernardi, Francesco, Bernardini, SOFIA ROBERTA, Biban, P., Bigi, M., Bossi, G., Bottone, U., Cardinale, A., Cardona, A., Castronari, R., Celandroni, A., Chiossi, M., Colleselli, P., Correra, A., Cortimiglia, M., D'Ascola, G., De Benedictis, F. M., de Martino, M., Dini, E., Dollfus, L., Domenici, R., Flacco, V., Verrotti, A., Gaetti, M. T., Gagliardi, L., Galli, Lorenzo, Giglio, P., Guala, A., Lanari, M., Lasagni, D., Lizzoli, C., Lombardi, E., Magnini, M., Mattei, R., Memmini, G., Mesirca, P., Gragnani, S., Migliozzi, L., Nunziata, F., Pecile, P., Pepe, G., Perferi, G., Peris, A., Perri, P. F., Pescollderungg, L., Pezzati, M., Poggi, G. M., Poggiolesi, C., Rapisardi, G., Ratta, L., Ricci, S., Ridi, F., Riva, MARCO ANDREA, Rizzo, L., Roman, B., Romano, F., Toffolo, A., Strano, M., Trapani, S., Valleriani, C., Vasarri, P., Vascotto, M., Vergine, G., Verini, M., Zorzi, C., Azzari, Chiara, Canessa, Clementina, Lippi, Francesca, Moriondo, Maria, Indolfi, Giuseppe, Nieddu, Francesco, Martini, Marco, de Martino, Maurizio, Castiglia, Paolo, Baldo, Vincenzo, Resti, Massimo, Italian Group for the Study of Invasive Bacterial Disease [, Lanari M., and ]

Subjects
Pediatrics, Children, Incidence, Neisseria meningitidis group B, Realtime PCR, Sensitivity, Vaccine, Adolescent, Age Distribution, Child, Child, Preschool, Hospitals, Pediatric, Humans, Immunization Schedule, Infant, Infant, Newborn, Italy, Meningitis, Meningococcal, Meningococcal Vaccines, Real-Time Polymerase Chain Reaction, Retrospective Studies, Sepsis, Neisseria meningitidis, Serogroup B, Molecular Medicine, Immunology and Microbiology (all), Veterinary (all), Public Health, Environmental and Occupational Health, Infectious Diseases, Disease, Neisseria meningitidis, medicine.disease_cause, Group B, Epidemiology, Medicine, Pediatric, Meningococcal, Incidence (epidemiology), Hospitals, Vaccination, Public Health, Meningitis, medicine.medical_specialty, Serogroup B, Immunology and Microbiology(all), MED/42 Igiene generale e applicata, Preschool, General Veterinary, General Immunology and Microbiology, business.industry, Environmental and Occupational Health, medicine.disease, Newborn, veterinary(all), business
Abstract

Neisseria meningitidis group B (MenB) is a leading cause of meningitis and sepsis. A new vaccine has been recently licensed. The aim of the present study was to evaluate the epidemiology of MenB disease in pediatric age and define the optimal age for vaccination. All patients aged 0–18 years admitted with a diagnosis of meningitis or sepsis to the 83 participating Italian pediatric hospitals were included in the study. Blood and/or cerebrospinal fluid (CSF) samples were tested by Realtime-PCR and/or culture. One hundred and thirty-six cases (mean age 5.0 years, median 2.7) of MenB disease were found. Among these, 96/136 (70.6%) were between 0 and 5 years, 61/136 (44.9%) were between 0 and 2 years. Among the latter, 39/61 (63.9%) occurred during the first year of life with highest incidence between 4 and 8 months. A case-fatality rate of 13.2% was found, with 27.8% cases below 12 months. Sepsis lethality was 24.4%. RT-PCR was significantly more sensitive than culture: 82 patients were tested at the same time by both methods, either in blood or in CSF; MenB was found by RT-PCR in blood or CSF in 81/82 cases (98.8%), culture identified 27/82 (32.9%) infections (Cohen's Kappa 0.3; McNemar's: p < 10−5). The study shows that the highest incidence of disease occurs in the first year of age, with a peak between 4 and 8 months of life; 30% of deaths occur before 12 months. The results suggest that the greatest prevention could be obtained starting MenB vaccination in the first months of life; a catch-up strategy up to the fifth year of life could be considered. Our results also confirm that Realtime PCR is significantly more sensitive than culture. In those countries where only isolate positive infections are counted as cases, the incidence of MenB infection results highly underestimated.

Published
2013

18. Towards phasing using high X-ray intensity

Author

Galli, Lorenzo, Son, Sang-Kil, Barends, Thomas R. M., White, Thomas A., Barty, Anton, Botha, Sabine, Boutet, Sébastien, Caleman, Carl, Doak, R. Bruce, Nanao, Max H., Nass, Karol, Shoeman, Robert L., Timneanu, Nicusor, Santra, Robin, Schlichting, Ilme, Chapman, Henry N., Galli, Lorenzo, Son, Sang-Kil, Barends, Thomas R. M., White, Thomas A., Barty, Anton, Botha, Sabine, Boutet, Sébastien, Caleman, Carl, Doak, R. Bruce, Nanao, Max H., Nass, Karol, Shoeman, Robert L., Timneanu, Nicusor, Santra, Robin, Schlichting, Ilme, and Chapman, Henry N.

Abstract

X-ray free-electron lasers (XFELs) show great promise for macromolecular structure determination from sub-micrometre-sized crystals, using the emerging method of serial femtosecond crystallography. The extreme brightness of the XFEL radiation can multiply ionize most, if not all, atoms in a protein, causing their scattering factors to change during the pulse, with a preferential ‘bleaching’ of heavy atoms. This paper investigates the effects of electronic damage on experimental data collected from a Gd derivative of lysozyme microcrystals at different X-ray intensities, and the degree of ionization of Gd atoms is quantified from phased difference Fourier maps. A pattern sorting scheme is proposed to maximize the ionization contrast and the way in which the local electronic damage can be used for a new experimental phasing method is discussed.

Published
2015
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19. Towards phasing using high X-ray intensity

Author

Galli, Lorenzo, primary, Son, Sang-Kil, additional, Barends, Thomas R. M., additional, White, Thomas A., additional, Barty, Anton, additional, Botha, Sabine, additional, Boutet, Sébastien, additional, Caleman, Carl, additional, Doak, R. Bruce, additional, Nanao, Max H., additional, Nass, Karol, additional, Shoeman, Robert L., additional, Timneanu, Nicusor, additional, Santra, Robin, additional, Schlichting, Ilme, additional, and Chapman, Henry N., additional

Published
2015
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20. Improving resolution in serial crystallography

Author

Ayyer, Kartik, primary, Yefanov, Oleksandr M., additional, Oberthuer, Dominik, additional, Chowdhury, Shatabdi, additional, Galli, Lorenzo, additional, Mariani, Valerio, additional, Basu, Shibom, additional, Coe, Jesse, additional, Conrad, Chelsie E., additional, Fromme, Raimund, additional, Doerner, Katerina, additional, Frank, Matthias, additional, James, Daniel, additional, Kupitz, Christopher, additional, Metz, Markus, additional, Nelson, Garrett, additional, Paulraj, Xavier L., additional, Beyerlein, Kenneth, additional, Schmidt, Marius, additional, Sarrou, Iosifina, additional, Spence, John C. H., additional, Weierstall, Uwe, additional, White, Thomas A., additional, Yang, Jayhow, additional, Zatsepin, Nadia A., additional, Zhao, Yun, additional, Liang, Mengning, additional, Aquila, Andrew, additional, Hunter, Mark S., additional, Robinson, Joseph S., additional, Koglin, Jason E., additional, Boutet, Sébastien, additional, Fromme, Petra, additional, Barty, Anton, additional, and Chapman, Henry N., additional

Published
2015
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22. Effects of self-seeding and crystal post-selection on the quality of Monte Carlo-integrated SFX data

Author

Barends, Thomas, primary, White, Thomas A., additional, Barty, Anton, additional, Foucar, Lutz, additional, Messerschmidt, Marc, additional, Alonso-Mori, Roberto, additional, Botha, Sabine, additional, Chapman, Henry, additional, Doak, R. Bruce, additional, Galli, Lorenzo, additional, Gati, Cornelius, additional, Gutmann, Matthias, additional, Koglin, Jason, additional, Markvardsen, Anders, additional, Nass, Karol, additional, Oberthur, Dominik, additional, Shoeman, Robert L., additional, Schlichting, Ilme, additional, and Boutet, Sébastien, additional

Published
2015
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24. Explosion dynamics of sucrose nanospheres monitored by time of flight spectrometry and coherent diffractive imaging at the split-and-delay beam line of the FLASH soft X-ray laser

Author

Rath, Asawari D., Timneanu, Nicusor, Maia, Filipe R. N. C., Bielecki, Johan, Fleckenstein, Holger, Iwan, Bianca, Svenda, Martin, Hasse, Dirk, Carlsson, Gunilla, Westphal, Daniel, Mühlig, Kerstin, Hantke, Max, Ekeberg, Tomas, Seibert, M. Marvin, Zani, Alessandro, Liang, Mengning, Stellato, Francesco, Kirian, Richard, Bean, Richard, Barty, Anton, Galli, Lorenzo, Nass, Karol, Barthelmess, Miriam, Aquila, Andrew, Toleikis, Sven, Treusch, Rolf, Roling, Sebastian, Wöstmann, Michael, Zacharias, Helmut, Chapman, Henry N., Bajt, Saša, DePonte, Daniel, Hajdu, Janos, Andreasson, Jakob, Rath, Asawari D., Timneanu, Nicusor, Maia, Filipe R. N. C., Bielecki, Johan, Fleckenstein, Holger, Iwan, Bianca, Svenda, Martin, Hasse, Dirk, Carlsson, Gunilla, Westphal, Daniel, Mühlig, Kerstin, Hantke, Max, Ekeberg, Tomas, Seibert, M. Marvin, Zani, Alessandro, Liang, Mengning, Stellato, Francesco, Kirian, Richard, Bean, Richard, Barty, Anton, Galli, Lorenzo, Nass, Karol, Barthelmess, Miriam, Aquila, Andrew, Toleikis, Sven, Treusch, Rolf, Roling, Sebastian, Wöstmann, Michael, Zacharias, Helmut, Chapman, Henry N., Bajt, Saša, DePonte, Daniel, Hajdu, Janos, and Andreasson, Jakob

Abstract

We use a Mach-Zehnder type autocorrelator to split and delay XUV pulses from the FLASH soft X-ray laser for triggering and subsequently probing the explosion of aerosolised sugar balls. FLASH was running at 182 eV photon energy with pulses of 70 fs duration. The delay between the pump-probe pulses was varied between zero and 5 ps, and the pulses were focused to reach peak intensities above 1016 W/cm2 with an off-axis parabola. The direct pulse triggered the explosion of single aerosolised sucrose nano-particles, while the delayed pulse probed the exploding structure. The ejected ions were measured by ion time of flight spectrometry, and the particle sizes were measured by coherent diffractive imaging. The results show that sucrose particles of 560-1000 nm diameter retain their size for about 500 fs following the first exposure. Significant sample expansion happens between 500 fs and 1 ps. We present simulations to support these observations.

Published
2014
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25. Trace phase detection and strain characterization from serial X-ray free-electron laser crystallography of a Pr0.5Ca0.5MnO3 powder

Author

Beyerlein, Kenneth R., primary, Jooss, Christian, additional, Barty, Anton, additional, Bean, Richard, additional, Boutet, Sébastien, additional, Dhesi, Sarnjeet S., additional, Doak, R. Bruce, additional, Först, Michael, additional, Galli, Lorenzo, additional, Kirian, Richard A., additional, Kozak, Joseph, additional, Lang, Michael, additional, Mankowsky, Roman, additional, Messerschmidt, Marc, additional, Spence, John C. H., additional, Wang, Dingjie, additional, Weierstall, Uwe, additional, White, Thomas A., additional, Williams, Garth J., additional, Yefanov, Oleksandr, additional, Zatsepin, Nadia A., additional, Cavalleri, Andrea, additional, and Chapman, Henry N., additional

Published
2014
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26. Explosion dynamics of sucrose nanospheres monitored by time of flight spectrometry and coherent diffractive imaging at the split-and-delay beam line of the FLASH soft X-ray laser

Author

Rath, Asawari D., primary, Timneanu, Nicusor, additional, Maia, Filipe R. N. C., additional, Bielecki, Johan, additional, Fleckenstein, Holger, additional, Iwan, Bianca, additional, Svenda, Martin, additional, Hasse, Dirk, additional, Carlsson, Gunilla, additional, Westphal, Daniel, additional, Mühlig, Kerstin, additional, Hantke, Max, additional, Ekeberg, Tomas, additional, Seibert, M. Marvin, additional, Zani, Alessandro, additional, Liang, Mengning, additional, Stellato, Francesco, additional, Kirian, Richard, additional, Bean, Richard, additional, Barty, Anton, additional, Galli, Lorenzo, additional, Nass, Karol, additional, Barthelmess, Miriam, additional, Aquila, Andrew, additional, Toleikis, Sven, additional, Treusch, Rolf, additional, Roling, Sebastian, additional, Wöstmann, Michael, additional, Zacharias, Helmut, additional, Chapman, Henry N., additional, Bajt, Saša, additional, DePonte, Daniel, additional, Hajdu, Janos, additional, and Andreasson, Jakob, additional

Published
2014
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27. Room-temperature macromolecular serial crystallography using synchrotron radiation

Author

Stellato, Francesco, primary, Oberthür, Dominik, additional, Liang, Mengning, additional, Bean, Richard, additional, Gati, Cornelius, additional, Yefanov, Oleksandr, additional, Barty, Anton, additional, Burkhardt, Anja, additional, Fischer, Pontus, additional, Galli, Lorenzo, additional, Kirian, Richard A., additional, Meyer, Jan, additional, Panneerselvam, Saravanan, additional, Yoon, Chun Hong, additional, Chervinskii, Fedor, additional, Speller, Emily, additional, White, Thomas A., additional, Betzel, Christian, additional, Meents, Alke, additional, and Chapman, Henry N., additional

Published
2014
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28. How Organizations Are Using Behavioral Science to Make Better People Decisions.

Author

Galli, Lorenzo

Subjects
BUSINESS enterprises, PSYCHOLOGY, DECISION making, DATA analytics, SOCIAL sciences
Abstract

The article provides information on the use of behavioural science by companies to make people decisions and achieve better results. More and more companies are making use of people analytics teams for analysis of organizational data. According to the author, many websites provide summaries of behavioral science research.

Published
2019

29. How Organizations Are Using Behavioral Science to Make Better People Decisions.

Author

Galli, Lorenzo

Subjects
PSYCHOLOGY, HUMAN behavior, EVIDENCE-based management, DECISION making, INDUSTRIAL psychology, BEHAVIORAL economics
Abstract

The article focuses on how organizations are using behavioral science to make better people decisions. Topics include Evidence-based management is about making people decisions based on the critical use the best available evidence; mentions Scientific research from organizational psychology, and behavioral economics tells how people tend to behave at work and why; and also mentions using behavioral science is giving organizations a solid rope to emerge from the muddy swamps of management myths.

Published
2018

30. Structure of a photosynthetic reaction centre determined by serial femtosecond crystallography

Author

Johansson, Linda C., Arnlund, David, Katona, Gergely, White, Thomas A., Barty, Anton, DePonte, Daniel P., Shoeman, Robert L., Wickstrand, Cecilia, Sharma, Amit, Williams, Garth J., Aquila, Andrew, Bogan, Michael J., Caleman, Carl, Davidsson, Jan, Doak, R. Bruce, Frank, Matthias, Fromme, Raimund, Galli, Lorenzo, Grotjohann, Ingo, Hunter, Mark S., Kassemeyer, Stephan, Kirian, Richard A., Kupitz, Christopher, Liang, Mengning, Lomb, Lukas, Malmerberg, Erik, Martin, Andrew V., Messerschmidt, Marc, Nass, Karol, Redecke, Lars, Seibert, M. Marvin, Sjoehamn, Jennie, Steinbrener, Jan, Stellato, Francesco, Wang, Dingjie, Wahlgren, Weixaio Y., Weierstall, Uwe, Westenhoff, Sebastian, Zatsepin, Nadia A., Boutet, Sebastien, Spence, John C. H., Schlichting, Ilme, Chapman, Henry N., Fromme, Petra, Neutze, Richard, Johansson, Linda C., Arnlund, David, Katona, Gergely, White, Thomas A., Barty, Anton, DePonte, Daniel P., Shoeman, Robert L., Wickstrand, Cecilia, Sharma, Amit, Williams, Garth J., Aquila, Andrew, Bogan, Michael J., Caleman, Carl, Davidsson, Jan, Doak, R. Bruce, Frank, Matthias, Fromme, Raimund, Galli, Lorenzo, Grotjohann, Ingo, Hunter, Mark S., Kassemeyer, Stephan, Kirian, Richard A., Kupitz, Christopher, Liang, Mengning, Lomb, Lukas, Malmerberg, Erik, Martin, Andrew V., Messerschmidt, Marc, Nass, Karol, Redecke, Lars, Seibert, M. Marvin, Sjoehamn, Jennie, Steinbrener, Jan, Stellato, Francesco, Wang, Dingjie, Wahlgren, Weixaio Y., Weierstall, Uwe, Westenhoff, Sebastian, Zatsepin, Nadia A., Boutet, Sebastien, Spence, John C. H., Schlichting, Ilme, Chapman, Henry N., Fromme, Petra, and Neutze, Richard

Abstract

Serial femtosecond crystallography is an X-ray free-electron-laser-based method with considerable potential to have an impact on challenging problems in structural biology. Here we present X-ray diffraction data recorded from microcrystals of the Blastochloris viridis photosynthetic reaction centre to 2.8 angstrom resolution and determine its serial femtosecond crystallography structure to 3.5 angstrom resolution. Although every microcrystal is exposed to a dose of 33MGy, no signs of X-ray-induced radiation damage are visible in this integral membrane protein structure.

Published
2013
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31. Structure of a photosynthetic reaction centre determined by serial femtosecond crystallography

Author

Johansson, Linda C., primary, Arnlund, David, additional, Katona, Gergely, additional, White, Thomas A., additional, Barty, Anton, additional, DePonte, Daniel P., additional, Shoeman, Robert L., additional, Wickstrand, Cecilia, additional, Sharma, Amit, additional, Williams, Garth J., additional, Aquila, Andrew, additional, Bogan, Michael J., additional, Caleman, Carl, additional, Davidsson, Jan, additional, Doak, R Bruce, additional, Frank, Matthias, additional, Fromme, Raimund, additional, Galli, Lorenzo, additional, Grotjohann, Ingo, additional, Hunter, Mark S., additional, Kassemeyer, Stephan, additional, Kirian, Richard A., additional, Kupitz, Christopher, additional, Liang, Mengning, additional, Lomb, Lukas, additional, Malmerberg, Erik, additional, Martin, Andrew V., additional, Messerschmidt, Marc, additional, Nass, Karol, additional, Redecke, Lars, additional, Seibert, M Marvin, additional, Sjöhamn, Jennie, additional, Steinbrener, Jan, additional, Stellato, Francesco, additional, Wang, Dingjie, additional, Wahlgren, Weixaio Y., additional, Weierstall, Uwe, additional, Westenhoff, Sebastian, additional, Zatsepin, Nadia A., additional, Boutet, Sébastien, additional, Spence, John C.H., additional, Schlichting, Ilme, additional, Chapman, Henry N., additional, Fromme, Petra, additional, and Neutze, Richard, additional

Published
2013
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33. Trace phase detection and strain characterization from serial X-ray free-electron laser crystallography of a Pr0.5Ca0.5MnO3powder

Author

Beyerlein, Kenneth R., Jooss, Christian, Barty, Anton, Bean, Richard, Boutet, Sébastien, Dhesi, Sarnjeet S., Doak, R. Bruce, Först, Michael, Galli, Lorenzo, Kirian, Richard A., Kozak, Joseph, Lang, Michael, Mankowsky, Roman, Messerschmidt, Marc, Spence, John C. H., Wang, Dingjie, Weierstall, Uwe, White, Thomas A., Williams, Garth J., Yefanov, Oleksandr, Zatsepin, Nadia A., Cavalleri, Andrea, and Chapman, Henry N.

Abstract

We report on the analysis of virtual powder-diffraction patterns from serial femtosecond crystallography (SFX) data collected at an X-ray free-electron laser. Different approaches to binning and normalizing these patterns are discussed with respect to the microstructural characteristics which each highlights. Analysis of SFX data from a powder of Pr0.5Ca0.5MnO3in this way finds evidence of other trace phases in its microstructure which was not detectable in a standard powder-diffraction measurement. Furthermore, a comparison between two virtual powder pattern integration strategies is shown to yield different diffraction peak broadening, indicating sensitivity to different types of microstrain. This paper is a first step in developing new data analysis methods for microstructure characterization from serial crystallography data.

Published
2015
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35. Analysis of XFEL serial diffraction data from individual crystalline fibrils

Author

Wojtas, David H., Ayyer, Kartik, Liang, Mengning, Mossou, Estelle, Romoli, Filippo, Seuring, Carolin, Beyerlein, Kenneth, Bean, Richard J., Morgan, Andrew, Oberthuer, Dominik, Fleckenstein, Holger, Heymann, Michael, Gati, Cornelius, Yefanov, Oleksandr, Barthelmess, Miriam, Ornithopoulou, Eirini, Galli, Lorenzo, Xavier, P. Lourdu, Ling, Wai Li, Frank, Matthias, Yoon, Chun Hong, White, Thomas A., Bajt, Saša, Mitraki, Anna, Boutet, Sebastien, Aquila, Andrew, Barty, Anton, Forsyth, V. Trevor, Chapman, Henry N., and Millane, Rick P.

Subjects
3. Good health
Abstract

IUCrJ 4(6), 795 - 811 (2017). doi:10.1107/S2052252517014324, Serial diffraction data collected at the Linac Coherent Light Source from crystalline amyloid fibrils delivered in a liquid jet show that the fibrils are well oriented in the jet. At low fibril concentrations, diffraction patterns are recorded from single fibrils; these patterns are weak and contain only a few reflections. Methods are developed for determining the orientation of patterns in reciprocal space and merging them in three dimensions. This allows the individual structure amplitudes to be calculated, thus overcoming the limitations of orientation and cylindrical averaging in conventional fibre diffraction analysis. The advantages of this technique should allow structural studies of fibrous systems in biology that are inaccessible using existing techniques., Published by IUCr, Chester

36. Algoritmi di ricostruzione di immagini per sistemi a microonde dedicati all'imaging della mammella

Author

Galli, Lorenzo, thesis supervisor: Lanconelli, Nico, Galli, Lorenzo, and thesis supervisor: Lanconelli, Nico

Abstract

Lo scopo di questa tesi è quello di presentare alcuni degli algoritmi di ricostruzione utilizzati nei sistemi a microonde per l'imaging della mammella. Sebbene, la mammografia a raggi X sia la tecnica che a oggi presenta le migliori caratteristiche per la rilevazione di lesioni tumorali ai tessuti del seno, la compressione necessaria nel processo di imaging e la probabilità non trascurabile di falsi negativi hanno portato alla ricerca e allo sviluppo di metodi alternativi di imaging alla mammella. Tra questi, l'imaging attraverso microonde sfrutta la differenza delle proprietà dielettriche tra tessuto sano e tumorale. Nel seguente elaborato verrà descritto come è ottenuta l'immagine dell'interno della mammella attraverso gli algoritmi di ricostruzione utilizzati da vari prototipi di sistemi di imaging. Successivamente verranno riportati degli esempi che mostrano i risultati ottenuti attraverso simulazioni o, quando possibile, in ambiente clinico.

37. Algoritmi di ricostruzione di immagini per sistemi a microonde dedicati all'imaging della mammella

Author

Galli, Lorenzo, thesis supervisor: Lanconelli, Nico, Galli, Lorenzo, and thesis supervisor: Lanconelli, Nico

Abstract

Lo scopo di questa tesi è quello di presentare alcuni degli algoritmi di ricostruzione utilizzati nei sistemi a microonde per l'imaging della mammella. Sebbene, la mammografia a raggi X sia la tecnica che a oggi presenta le migliori caratteristiche per la rilevazione di lesioni tumorali ai tessuti del seno, la compressione necessaria nel processo di imaging e la probabilità non trascurabile di falsi negativi hanno portato alla ricerca e allo sviluppo di metodi alternativi di imaging alla mammella. Tra questi, l'imaging attraverso microonde sfrutta la differenza delle proprietà dielettriche tra tessuto sano e tumorale. Nel seguente elaborato verrà descritto come è ottenuta l'immagine dell'interno della mammella attraverso gli algoritmi di ricostruzione utilizzati da vari prototipi di sistemi di imaging. Successivamente verranno riportati degli esempi che mostrano i risultati ottenuti attraverso simulazioni o, quando possibile, in ambiente clinico.

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