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7. Sulfur-Containing Compounds from Plants

Author

Castro, Vera, Carpena, M., Fraga-Corral, M., Lopez-Soria, A., Garcia-Perez, P., Barral-Martinez, M., Perez-Gregorio, R., Cao, Hui, Simal-Gandara, J., Prieto, M. A., Carocho, Márcio, editor, Heleno, Sandrina A., editor, and Barros, Lillian, editor

Published
2023
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32. List of Contributors

Author

Arce, L., primary, Capanoglu, Esra, additional, Caporaso, Nicola, additional, Cardador, M.J., additional, Casal, Susana, additional, De Souza, Djenaine, additional, Economou, Anastasios, additional, ElMasry, Gamal, additional, Esteki, M., additional, Gou, Pere, additional, Guldiken, Burcu, additional, Jurado-Campos, N., additional, Kamiloglu, Senem, additional, Karliga, Simge, additional, Karoui, Romdhane, additional, Kjellerup, Birthe V., additional, Liu, Junyan, additional, Luo, Yuting, additional, Magwaza, L.S., additional, Martín-Gómez, A., additional, Martins, Fernanda C.O.L., additional, Marx, Ítala M.G., additional, Melini, Francesca, additional, Melini, Valentina, additional, Ncama, K., additional, Ozdal, Tugba, additional, Pereira, José A., additional, Peres, António M., additional, Raina-Fulton, Renata, additional, Sentanin, Michelle A., additional, Simal-Gandara, J., additional, Tomas, Merve, additional, Veloso, Ana C.A., additional, Wu, Long, additional, Xu, Zhenbo, additional, and Yolci-Omeroglu, Perihan, additional

Published
2021
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43. Enhancing the detection of Dinophysis spp. using electrochemical genosensors

Author

Pereira, Eduarda, Silva, Aurora, Morais, Stephanie L., Costa-Rama, Estefanía, Moreira, Patrícia R., Fraga-Corral, M., Torrado, Ana M., Rodríguez, Francisco, Barros, Piedade, Cruz, Agostinho, Delerue-Matos, Cristina, Prieto, M. A., Simal-Gandara, J., Silva, Nádia F. D., Santos, Marlene, and Barroso, M. Fátima

Subjects
electrochemistry, dinoflagellates, molecular biology, Dinophysis spp, genosensor
Abstract

Background: Harmful algal blooms (HABs) pose a significant threat to the environment and public health. These blooms are defined by an accumulation of microscopic algae in water, and they can occur in lakes, rivers, estuaries, or coastal areas. Factors like the unregulated runoff of agricultural and industrial wastes into the aquatic environment [1] are believed to have transformed these ecosystems into favorable habitats for algae growth and proliferation [2]. As a result, the frequency of these blooms is rising worldwide. Although these blooms are mostly harmless, certain species, namely dinoflagellates from the genus Dinophysis, produce toxins that pose a risk for human health [3]. Therefore, the need for technological developments towards fast and precise detection of these toxin-producing microalgae is critical to prevent socioeconomical damages, as well as to assess the ecological status of marine ecosystems [1]. Objective: In this work, an analytical approach based on an electrochemical genosensor device was developed to create a low-cost platform able to detect two dinoflagellate species from the genus Dinophysis: D.acuminata and D.acuta. Methods: The design of the DNA-based sensor involved three key steps: i) Sensing phase: consisted by a mixed self-assembled monolayer composed by a linear DNA capture probe and mercaptohexanol onto the disposable screen-printed gold electrodes surface; ii) Hybridization of complementary DNA sequence by using a sandwich format assay with enzymatic labels and iii) Electrochemical detection by chronoamperometry using an enzymatic scheme to amplify the electrochemical signal. Results: The best analytical conditions used to study the relationship between electrochemical signal and DNA target concentration, to produce the best electrochemical genosensor device. Molecular biology tools, namely Polymerase Chain Reaction (PCR), will be used for further validation of the electrochemical genosensor to confirm its reliability. Conclusions: These advancements in analytical technologies contribute to the ongoing efforts in environmental management and public health protection by providing effective means for detecting and mitigating the risks associated with HABs. Further research and widespread implementation of these methods are required to ensure the safety and sustainability of aquatic ecosystems, safeguard public health, and facilitate proactive environmental management practices., Proceedings of Research and Practice in Allied and Environmental Health, Vol. 1 N.º 1 (2023): I Jornadas do CISA: 'Jovens Com a Ciência'

Published
2023
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45. Untargeted metabolomics and in vitro functional analysis unravel the intraspecific bioactive potential of flowers from underexplored Camellia japonica cultivars facing their industrial application

Author

Pereira, A. G., Cassani, L., Oludemi, T., Chamorro, F., Calhelha, R. C., Prieto, M. A., Barros, L., Simal-Gandara, J., Lucini, Luigi, Garcia-Perez, P., Lucini L. (ORCID:0000-0002-5133-9464), Pereira, A. G., Cassani, L., Oludemi, T., Chamorro, F., Calhelha, R. C., Prieto, M. A., Barros, L., Simal-Gandara, J., Lucini, Luigi, Garcia-Perez, P., and Lucini L. (ORCID:0000-0002-5133-9464)

Abstract

The Camellia genus comprises a vast array of underexplored medicinal plants that merit a systematic valorization to exploit their potential as natural sources of phytochemicals with associated health-promoting properties. In this work, flower extracts from eight poorly characterized Camellia japonica L. cultivars were subjected to polyphenol profiling through untargeted metabolomics combined with in vitro functional analysis. Anthocyanins, mostly represented by cyanidin 3-O-glycosides, flavones, and flavonols, were found as the major constituents of C. japonica flowers, together with hydroxycinnamic acids, tyrosols, alkylphenols, and stilbenes, which were detected for the first time in this species. The application of multivariate statistics revealed a flower color-dependent fingerprint of C. japonica cultivars, featuring anthocyanins and other flavonoids as metabolite markers associated with color-flowered cultivars with respect to white-flowered ones. The accumulation of anthocyanins, especially reported in ‘Eugenia de Montijo’ flowers, was highly correlated with the cytotoxic and anti-inflammatory properties of the derived extracts, including AGS, Caco-2, and MCF7 cancer cell lines. Moreover, the flavones accumulation reported in ‘Carolyn Tuttle’ extracts was also associated with high rates of free-radical scavenging activity, as well as a potent cytotoxicity against the Caco-2 cell line. In general, C. japonica anthocyanin-enriched flower extracts were revealed as promising candidates for the industrial production of polyphenols with associated biological activities of high interest for critical sectors in the food, pharmaceutical, and cosmetic industries.

Published
2023

46. Algal nutraceuticals: A perspective on metabolic diversity, current food applications, and prospects in the field of metabolomics

Author

Garcia-Perez, P., Cassani, L., Garcia-Oliveira, P., Xiao, J., Simal-Gandara, J., Prieto, M. A., Lucini, Luigi, Lucini L. (ORCID:0000-0002-5133-9464), Garcia-Perez, P., Cassani, L., Garcia-Oliveira, P., Xiao, J., Simal-Gandara, J., Prieto, M. A., Lucini, Luigi, and Lucini L. (ORCID:0000-0002-5133-9464)

Abstract

The current consumers’ demand for food naturalness is urging the search for new functional foods of natural origin with enhanced health-promoting properties. In this sense, algae constitute an underexplored biological source of nutraceuticals that can be used to fortify food products. Both marine macroalgae (or seaweeds) and microalgae exhibit a myriad of chemical constituents with associated features as a result of their primary and secondary metabolism. Thus, primary metabolites, especially polysaccharides and phycobiliproteins, present interesting properties to improve the rheological and nutritional properties of food matrices, whereas secondary metabolites, such as polyphenols and xanthophylls, may provide interesting bioactivities, including antioxidant or cytotoxic effects. Due to the interest in algae as a source of nutraceuticals by the food and related industries, novel strategies should be undertaken to add value to their derived functional components. As a result, metabolomics is considered a high throughput technology to get insight into the full metabolic profile of biological samples, and it opens a wide perspective in the study of algae metabolism, whose knowledge is still little explored. This review focuses on algae metabolism and its applications in the food industry, paying attention to the promising metabolomic approaches to be developed aiming at the functional characterization of these organisms.

Published
2023

49. The Bioactivity of Green-Synthesized Noble Metal Nanoparticles Against Streptomycin-Resistant Bacillus Sp. Is Enhanced by Bioconjugation with Streptomycin

Author

Ramasami, N., primary, Dhayalan, M., additional, Selvaraj, M., additional, Mohammed Riyaz, S. U., additional, Palani, Perumal, additional, Kanthapazham, Raja kumar, additional, Zherebtsov, D., additional, Mejuto, J. C., additional, Simal-Gandara, J., additional, and Cid-Samamed, A., additional

Published
2023
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