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Present article describes about the preparation of Nanofiltration (NF) composite membranes by a simple chemical treatment to the polymeric membrane composed of Poly (isobutylene-alt-maleic anhydride). After composition, anhydride functionality was converted to diacid functionality by hydrolysis using sodium hydroxide solution. Further, membrane was characterized by ATR-IR, DSC and SEM. Charge confirmation was done by IEC. Water uptake and contact angle analysis was carried out to study the hydrophilicity of the membrane. The performance study was carried out by using NaCl and CaCl2 solutions. Membranes were having higher charge density, which has resulted in better performance in terms of rejection of CaCl2 and NaCl solutions. The main focus of this research is to evaluate the effect of alkali treatment on Donnan and size exclusion mechanism during membrane filtration.