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6. Polarity-sensitive pyrene fluorescent probes for multi-organelle imaging in living cells.

Author

Yang, Linshuai, Cao, Ying, Bu, Xiaoyu, Peng, Fei, Ai, Qi, and Gao, Baoxiang

Subjects
FLUORESCENT probes, CELL imaging, AZETIDINE, PYRENE, ORGANELLES
Abstract

Polarity-sensitive probes (PAS) were synthesized through the attachment of azetidine and sulfonyl substituents to the pyrene fluorescent core. The emission peaks and fluorescence lifetimes of these PAS probes exhibit high sensitivity to polarity, enabling the visualization of microenvironmental characteristics and dynamics across multiple organelles. [ABSTRACT FROM AUTHOR]

Published
2025
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12. Expanding super-resolution imaging versatility in organisms with multi-confocal image scanning microscopy.

Author

Ren, Wei, Guan, Meiling, Liang, Qianxi, Li, Meiqi, Jin, Boya, Duan, Guangxing, Zhang, Liya, Ge, Xichuan, Xu, Hong, Hou, Yiwei, Gao, Baoxiang, Sodmergen, and Xi, Peng

Subjects
HIGH resolution imaging, LASER microscopy, TISSUES, MICROSCOPY, MITOCHONDRIA
Abstract

Resolving complex three-dimensional (3D) subcellular dynamics noninvasively in live tissues demands imaging tools that balance spatiotemporal resolution, field-of-view and phototoxicity. Image scanning microscopy (ISM), as an advancement of confocal laser scanning microscopy, provides a 2-fold 3D resolution enhancement. Nevertheless, the relatively low imaging speed has been the major obstacle for ISM to be further employed in in vivo imaging of biological tissues. Our proposed solution, multi-confocal image scanning microscopy (MC-ISM), aims to overcome the limitations of existing techniques in terms of spatiotemporal resolution balancing by optimizing pinhole diameter and pitch, eliminating out-of-focus signals, and introducing a frame reduction reconstruction algorithm. The imaging speed is increased by 16 times compared with multifocal structured illumination microscopy. We further propose a single-galvo scan, akin to the Archimedes spiral in spinning disk confocal systems, to ensure a high-speed and high-accuracy scan without the galvanometer's inertial motion. Benefitting from its high photon efficiency, MC-ISM allows continuous imaging of mitochondria dynamics in live cells for 1000 frames without apparent phototoxicity, reaching an imaging depth of 175 μm. Noteworthy, MC-ISM enables the observation of the inner membrane structure of living mitochondria in Arabidopsis hypocotyl for the first time, demonstrating its outstanding performance. [ABSTRACT FROM AUTHOR]

Published
2024
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14. Recent advances in FRET probes for mitochondrial imaging and sensing.

Author

Peng, Fei, Ai, Xiangnan, Sun, Jing, Yang, Linshuai, and Gao, Baoxiang

Subjects
FLUORESCENCE resonance energy transfer, ORGANELLES, MOLECULAR probes, MITOCHONDRIA
Abstract

Mitochondria, as essential organelles in cells, play a crucial role in cellular growth and apoptosis. Monitoring mitochondria is of great importance, as mitochondrial dysfunction is often considered a hallmark event of cell apoptosis. Traditional fluorescence probes used for mitochondrial imaging and sensing are mostly intensity-based and are susceptible to factors such as concentration, the probe environment, and fluorescence intensity. Probes based on fluorescence resonance energy transfer (FRET) can effectively overcome external interference and achieve high-contrast imaging of mitochondria as well as quantitative monitoring of mitochondrial microenvironments. This review focuses on recent advances in the application of FRET-based probes for mitochondrial structure imaging and microenvironment sensing. [ABSTRACT FROM AUTHOR]

Published
2024
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16. Peripheral lymphocyte count defines the clinical phenotypes and prognosis in patients with anti‐MDA5‐positive dermatomyositis

Author

Jin, Qiwen, primary, Fu, Lijun, additional, Yang, Hongxia, additional, Chen, Xixia, additional, Lin, Sang, additional, Huang, Zhenguo, additional, Gao, Baoxiang, additional, Tian, Xiaolan, additional, Jiang, Wei, additional, Shu, Xiaoming, additional, Lu, Xin, additional, Wang, Guochun, additional, and Peng, Qinglin, additional

Published
2023
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23. Biocompatible Fluorescent Probe with the Aggregation-induced Emission Characteristic for Live Cell Imaging

Author

Xi Yanan, Gao Wei, Chen Jingtian, Zhang Shuchen, Yang Tao, and Gao Baoxiang

Subjects
Fluorescent probe, aggregation-induced emission (AIE), photophysical properties, cellular imaging, Engineering (General). Civil engineering (General), TA1-2040
Abstract

A new kind of biocompatible probe (PEG-TPEFE) with aggregation-induced emission (AIE) characteristic was reported, which was synthesized from fluorene, tetraphenylethylene (TPE) and polyethylene glycol monomethyle ether 1000 (PEG1000). PEGTPEFE can aggregate into nanosphere and emit strong blue fluorescence in aqueous media. Cytotoxicity assay reveals low cytotoxicity and excellent biocompatibility of this nanoprobe. This nanoprobe was internalized and accumulated by live cells and shown high photostability.

Published
2016
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28. Fluorescent probes based on multifunctional encapsulated perylene diimide dyes for imaging of lipid droplets in live cells.

Author

Li, Xinwei, Yang, Zikang, Bian, Jiqing, Fu, Mingyang, Zhang, Yan, Jiang, Nan, Qiao, Yanjun, Chen, Hua, and Gao, Baoxiang

Subjects
FLUORESCENT probes, PERYLENE, IMIDES, FLUORESCENCE quenching, DYES & dyeing
Abstract

Due to their strong hydrophobicity and the aggregation-caused quenching effect, the application of perylene diimide (PDI) dyes in biological and medicinal fields lags far behind that of other dyes. Based on a multifunctional encapsulation strategy, we prepared isopropylphenyl sulfone encapsulated PDI dyes (SFPDIs). The four hydrophilic sulfone groups on the bay position of the PDIs not only effectively inhibit the fluorescence quenching caused by π-aggregation but also endow the SFPDIs with good live-cell permeability. The six lipophilic isopropylphenyl groups encapsulate PDI emitters and confer SFPDIs with excellent lipid droplet-targeting ability. Furthermore, the strong electron-withdrawing sulfone groups give the PDIs excellent anti-oxidation ability. [ABSTRACT FROM AUTHOR]

Published
2022
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29. CT-guided microcoil implantation for localizing pulmonary ground-glass nodules: feasibility and accuracy of oblique approach for lesions difficult to access on axial images

Author

Gao Baoxiang, Chen He, Yang Minxing, Huang Zhenguo, Sun Hong-Liang, Li Chuandong, and Chen Xiaoliang

Subjects
Male, medicine.medical_specialty, Lung Neoplasms, Radiography, Prosthesis Implantation, Microcoil, Radiography, Interventional, 030218 nuclear medicine & medical imaging, 03 medical and health sciences, 0302 clinical medicine, Fiducial Markers, X ray computed, Humans, Medicine, Radiology, Nuclear Medicine and imaging, Retrospective Studies, Full Paper, Thoracic Surgery, Video-Assisted, business.industry, General Medicine, Middle Aged, Cardiothoracic surgery, 030220 oncology & carcinogenesis, Feasibility Studies, Multiple Pulmonary Nodules, Female, Tomography, Tomography, X-Ray Computed, business, Nuclear medicine, Fiducial marker
Abstract

Objective: (1) To evaluate the value of CT-guided microcoil implantation for localizing pulmonary ground-glass nodules (GGNs) before video-assisted thoracoscopic surgery (VATS). (2) To evaluate the feasibility, safety and accuracy of cephalic-caudal oblique approach for lesions difficult to access on axial images owing to overlying bony structures, large vessels or interlober fissures. Methods: From June 2016 to March 2019, all patients with GGNs resected by VATS after marking using CT-guided microcoil implantation in China-Japan friendship hospital were enrolled and clinical and imaging data were retrospectively analyzed. According to the microcoil marked path, the GGNs were divided into cephalic-caudal oblique group (oblique group) and non-oblique group. The success rate of marking, the time required for marking and the incidence of complications between the two groups were compared. Results: 258 GGNs from 215 consecutive patients were included in this study. The diameter of GGNs was 1.22 ± 0.50 cm, and the shortest distance from GGNs to the pleura was 1.56 ± 1.09 cm. All 258 GGNs were successfully resected by VATS under the guidance of implanted microcoils, and no case was converted to thoracotomy. During CT-guided microcoil implantation, cephalic- caudal oblique approach was taken in 56 GGNs (oblique group) to avoid bone, interlobar fissure and blood vessels. The time required for marking was significantly longer for oblique group compared with non-oblique group (16.6 ± 2.4 vs. 13.1 ± 1.9 min, pConclusion: CT-guided microcoil implantation can effectively guide VATS to resect GGNs. For GGNs difficult to access on axial images, CT-guided cephalic-caudal oblique approach is feasible, safe, and accurate. Advances in knowledge: CT-guided microcoil implantation can effectively guide VATS to resect GGNs. The marked path with cephalic-caudal obliquity can effectively avoid bone, interlobar fissure and blood vessels, successfully mark GGNs difficult to access on axial images, while keeping the distance from the pleura to the lesion on the marked path as short as possible at the same time.

Published
2020
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30. Value of whole-body magnetic resonance imaging for screening multifocal osteonecrosis in patients with polymyositis/dermatomyositis

Author

Yang Minxing, Huang Zhenguo, Wang Guo-chun, Yan Ran, Yin Li-guo, Chen Xiaoliang, Chen He, Gao Baoxiang, Queenie Chan, and Shi Kai-ning

Subjects
Adult, Male, medicine.medical_specialty, Adolescent, Radiography, Whole body imaging, Polymyositis, Dermatomyositis, 030218 nuclear medicine & medical imaging, 03 medical and health sciences, Young Adult, 0302 clinical medicine, medicine, Humans, Radiology, Nuclear Medicine and imaging, Whole Body Imaging, Child, Letter to the Editor, Aged, Retrospective Studies, 030203 arthritis & rheumatology, Aged, 80 and over, medicine.diagnostic_test, Full Paper, business.industry, Medical record, Osteonecrosis, Retrospective cohort study, Magnetic resonance imaging, General Medicine, Middle Aged, medicine.disease, Magnetic Resonance Imaging, Surgery, Early Diagnosis, Coronal plane, Female, Radiology, business
Abstract

To assess the value of coronal short-tau inversion recovery whole-body MRI (STIR-WBMRI) for screening osteonecrosis in patients with polymyositis (PM)/dermatomyositis (DM).The imaging and medical records of 129 patients with PM/DM who met the Bohan and Peter diagnostic criteria were retrospectively analyzed. STIR-WBMRI was performed in all patients. 18 patients had follow-up STIR-WBMRI. 12 patients underwent regional knee and/or hip MRI while 25 patients underwent radiography of the lower extremities.STIR-WBMRI detected osteonecrosis in 15 (11.6%) patients. 38 joints were affected (mean, 2.5 per patient; range, 1-5 joints). Of the 38 joints affected by osteonecrosis, 33 had no clinical symptoms. Among the 12 patients who underwent regional MRI, STIR-WBMRI detected all 10 osteonecrotic sites seen on the regional MRI. The location, shape and size of the osteonecrotic lesions revealed on regional MRI were in accordance with those displayed on STIR-WBMRI. Of the 15 patients with osteonecrosis, 6 performed routine radiography of the affected joints and revealed no osteonecrotic lesions. Follow-up WBMRI detected new osteonecrosis in two patients whose first WBMRI revealed that there was no osteonecrosis in any skeleton.In addition to displaying muscle inflammation, STIR-WBMRI can efficiently detect early multifocal osteonecrosis in the whole bodies of patients with PM/DM. Advances in knowledge: In patients with PM/DM, WBMRI which takes 12-15 min can display muscular involvement and detect early multisite osteonecrosis in the whole body at the same time. Osteonecrotic lesions revealed by WBMRI are in accordance with those displayed on regional WBMRI.

Published
2017

36. ROI for outlining an entire tumor is a reliable approach for quantification of lung cancer tumor vascular parameters using CT perfusion

Author

Ma,Ensen, Ren,An, Gao,Baoxiang, Yang,Minxing, Zhao,Qichao, Wang,Wu, Li,Kefeng, Ma,Ensen, Ren,An, Gao,Baoxiang, Yang,Minxing, Zhao,Qichao, Wang,Wu, and Li,Kefeng

Abstract

Ensen Ma,1 An Ren,1 Baoxiang Gao,1 Minxing Yang,1 Qichao Zhao,1 Wu Wang,1 Kefeng Li21Department of Radiology, China–Japan Friendship Hospital, Beijing, People’s Republic of China; 2Department of Medicine, School of Medicine, University of California, San Diego, CA, USA Objective: To investigate the effect of position and size of tumor region of interest (ROI) on the estimation of lung cancer vascular parameters using 256-slice computed tomography (CT) perfusion.Methods: After institutional review board approval and written informed consent, 16 men and 11 women with lung cancer were enrolled in this CT perfusion study. Perfusion, blood volume, and peak enhancement were determined for 60 or 120 mm2 circular ROIs placed at the edge, center, and around (outlining) the visible tumor. Average values were obtained by performing ROI analysis twice by the same observers without any procedural changes.Results: Perfusion, blood volume, and peak enhancement measurements were substantially higher at the edge than at the center for both 60 and 120 mm2 ROIs (all P<0.05). Measurements varied substantially depending on the ROI size. Perfusion, blood volume, and peak enhancement for the ROIs outlining tumor were intermediate between those at the tumor edge and center. There were significant correlations between median values and interquartile ranges as follows; perfusion (12.51 [7.91–28.10] mL·min−1·100 mL−1), blood volume (29.31 [21.82–37.65] mL·100 g−1), peak enhancement (12.93 [2.42–22.50]) for the ROIs outlining the tumor, and microvascular density ([19.43±8.78] vessels/0.74 mm2), respectively (r values were 0.732, 0.590, and 0.544 respectively, all P<0.05).Conclusion: Spatial and size selection of ROI significantly affects CT perfusion analysis. ROI outlining of entire tumor provides efficient and reliable measurements for clinical as

Published
2016

39. Multicolor Tuning of Perylene Diimides Dyes for Targeted Organelle Imaging In Vivo

Author

Yang, Zikang, Li, Xinwei, Sun, Tingting, Bian, Jiqing, Bu, Xiaoyu, Ge, Xichuan, Sun, Jing, Liu, Zugang, Xie, Zhigang, Xi, Peng, Ai, Qi, Wei, Chao, and Gao, Baoxiang

Abstract

The adjustment of the emission wavelengths and cell permeability of the perylene diimides (PDI) for multicolor cell imaging is a great challenge. Herein, based on a bay-region substituent engineering strategy, multicolor perylene diimides (MCPDI) were rationally designed and synthesized by introducing azetidine substituents on the bay region of PDIs. With the fine-tuned electron-donating ability of the azetidine substituents, these MCPDIshowed high brightness, orange, red, and near infrared (NIR) fluorescence along with Stokes shifts increasing from 35 to 110 nm. Interestingly, azetidine substituents distorted to the plane of the MCPDIdyes, and the twist angle of monosubstituted MCPDIwas larger than that of disubstituted MCPDI, which might efficiently decrease their π–π stacking. Moreover, all of these MCPDIdyes were cell-permeable and selectively stained various organelles for multicolor imaging of multiple organelles in living cells. Two-color imaging of lipid droplets (LDs) and other organelles stained with MCPDIdyes was performed to reveal the interaction between the LDs and other organelles in living cells. Furthermore, a NIR-emitting MCPDIdye with a mitochondria-targeted characteristic was successfully applied for tumor-specific imaging. The facile synthesis, excellent stability, high brightness, tunable fluorescence emission, and Stokes shifts make these MCPDIpromising fluorescent probes for biological applications.

Published
2024
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40. Fluorescence Lifetime Super-Resolution Imaging Unveil the Dynamic Relationship between Mitochondrial Membrane Potential and Cristae Structure Using the Förster Resonance Energy Transfer Strategy

Author

Peng, Fei, Ai, Xiangnan, Sun, Jing, Ge, Xichuan, Li, Meiqi, Xi, Peng, and Gao, Baoxiang

Abstract

Mitochondrial cristae, invaginations of the inner mitochondrial membrane (IMM) into the matrix, are the main site for the generation of ATP via oxidative phosphorylation, and mitochondrial membrane potential (MMP). Synchronous study of the dynamic relationship between cristae and MMP is very important for further understanding of mitochondrial function. Due to the lack of suitable IMM probes and imaging techniques, the dynamic relationship between MMP and cristae structure alterations remains poorly understood. We designed a pair of FRET-based molecular probes, with the donor (OR-LA) being rhodamine modified with mitochondrial coenzyme lipoic acid and the acceptor (SiR-BA) being silicon-rhodamine modified with a butyl chain, for simultaneous dynamic monitoring of mitochondrial cristae structure and MMP. The FRET process of the molecular pair in mitochondria is regulated by MMP, enabling more precise visualization of MMP through fluorescence intensity ratio and fluorescence lifetime. By combining FRET with FLIM super-resolution imaging technology, we achieved simultaneous dynamic monitoring of mitochondrial cristae structure and MMP, revealing that during the decline of MMP, there is a progression involving cristae dilation, fragmentation, mitochondrial vacuolization, and eventual rupture. Significantly, we successfully observed that the rapid decrease in MMP at the site of mitochondrial membrane rupture may be a critical factor in mitochondrial fragmentation. These data collectively reveal the dynamic relationship between cristae structural alterations and MMP decline, laying a foundation for further investigation into cellular energy regulation mechanisms and therapeutic strategies for mitochondria-related diseases.

Published
2024
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49. Comparison of Apparent Diffusion Coefficient and T2 Relaxation Time Variation Patterns in Assessment of Age and Disc Level Related Intervertebral Disc Changes

Author

Wu, Nan, primary, Liu, Hao, additional, Chen, Jun, additional, Zhao, Luo, additional, Zuo, Wei, additional, Ming, Yue, additional, Liu, Sen, additional, Liu, Jiaqi, additional, Su, Xinlin, additional, Gao, Baoxiang, additional, Tang, Zhiquan, additional, Qiu, Guixing, additional, Ma, Guolin, additional, and Wu, Zhihong, additional

Published
2013
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