Search

Your search keyword '"Gao, Hongdan"' showing total 9 results
Start Over Author "Gao, Hongdan"
9 results on '"Gao, Hongdan"'

2. A multiplex recombinase polymerase amplification assay combined with CRISPR/Cas12a for the detection of respiratory syncytial virus and respiratory adenovirus

Author

Gao Hongdan, Du Yao, Chai Qiang, Huang Meng, Liu Xiaorong, Xing Zhihao, and Ma Dongli

Subjects
Medicine (General), R5-920
Abstract

Objective Respiratory syncytial virus (RSV) and respiratory adenovirus (ADV) are two common pathogens that cause acute respiratory tract infections in children. We aimed to develop a rapid method for detecting both pathogens simultaneously. Methods The recombinase polymerase isothermal amplification (RPA) method was combined with the CRISPR/Cas detection system. The assay’s specificity and sensitivity were explored by designing RPA primers and CRISPR RNAs (crRNAs) through multi-sequence comparisons, optimizing the reaction conditions, and using a fluorescent reading device. The consistency of the test results of 160 clinical pharyngeal swab samples was studied using quantitative polymerase chain reaction (qPCR) results as a comparative control. Results RSV and ADV could be detected at levels as low as 10 4 copies/mL and 10 3 copies/mL, respectively, within 50 minutes with no cross-reactivity with other similar pathogens. For the clinical samples, compared with the qPCR method, the sensitivities for RSV and ADV were 98.1% and 91.4%, respectively, and the detection specificities were both 100%. The Kappa values were greater than 0.95, suggesting a high degree of consistency. Conclusion This method for detecting RSV and ADV is rapid, sensitive, and specific. It can accurately detect mixed infections in a timely manner, making it suitable for use in areas with scarce healthcare resources.

Published
2024
Full Text
View/download PDF

6. Performance of targeted next-generation sequencing in the detection of respiratory pathogens and antimicrobial resistance genes for children

Author

Lin, Ruihong, primary, Xing, Zhihao, additional, Liu, Xiaorong, additional, Chai, Qiang, additional, Xin, Zefeng, additional, Huang, Meng, additional, Zhu, Chunqing, additional, Luan, Ce, additional, Gao, Hongdan, additional, Du, Yao, additional, Deng, Xuwen, additional, Zhang, Hetong, additional, and Ma, Dongli, additional

Published
2023
Full Text
View/download PDF

7. Additional file 4 of Comparison and development of a metagenomic next-generation sequencing protocol for combined detection of DNA and RNA pathogens in cerebrospinal fluid

Author

Jiang, Hanfang, Xing, Zhihao, Liu, Xiaorong, Chai, Qiang, Xin, Zefeng, Zhu, Chunqing, Lin, Ruihong, Deng, Xuwen, Cui, Dong, Gao, HongDan, and Ma, Dongli

Abstract

Additional file 4: Fig. S1. The microbial reads percentage of EBV samples and EV71 samples. A. The microbial reads percentage of EBV samples in methods Total DNA , Total NA ,Total RNA and WTA. B. The microbial reads percentage of EV71 samples in methods Total NA ,Total RNA and WTA. Fig. S2. NR, RPMratio and Coverage percent of target pathogens. Subfigure A/B/C shows NR/ RPMratio/coverage percentage of E. coli respectively comparing four sample pretreatment/nuclear acid extraction methods including Microbial DNA, Total DNA, Total NA and Total RNA. The red dots represent concentration of 106cfu/ml and the blue dots represent concentration of 5x104 cfu/ml. Subfigure D/E/F shows NR/ RPMratio/coverage percentage of SP comparing four sample pretreatment/nuclear acid extraction methods respectively. The red dots represent concentration of 106cfu/ml and the blue dots represent concentration of 5x104 cfu/ml. Subfigure G/H/I shows NR/RPMratio/coverage percentage of EBV respectively comparing four sample pretreatment/nuclear acid extraction methods including Total DNA, Total NA, Total RNA and WTA. The red dots represent concentration of 5x105 copies/ml and the blue dots represent concentration of 5x103 copies/ml. Subfigure J/K/L shows NR/RPMratio/coverage percentage of EV71 respectively comparing four sample pretreatment/nuclear acid extraction methods respectively. The red dots represent concentration of 5x105 copies/ml and the blue dots represent concentration of 5x103 copies/ml. Fig. S3. Genomic coverage of pathogens in CSF from encephalitis patients. A. Genomic coverage of Echovirus 30 in clinical CSF 0006. B. Genomic coverage of coxsackievirus B5 in clinical CSF 0010. Fig. S4. Genomic coverage of Streptococcus pneumoniae, Streptococcus thermophilus and Mogibacterium diversum in sample Microbial DNA-ILL -1. A. Genomic coverage of Streptococcus pneumoniae. B. Genomic coverage of Streptococcus thermophilus. C. Genomic coverage of Mogibacterium diversum.

Published
2022
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results