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In the current study, a series of 1,3,4-oxadiazole and 1,2,4-triazole derivatives linked to quinazolinone were designed and synthesized by using Phase Transfer Catalysis. The structures of the synthesized compounds were confirmed by their IR, 1H NMR, 13C NMR, and HR-ESI-MS spectroscopic data, and their in vitro antiproliferative activities against A549, Hela, MCF-7 as well as L929 were detected by using CCK-8 assay. The results demonstrated that most of the compounds showed better growth inhibitory effect than 5-FU on A549, Hela, and MCF-7 cell lines. Especially, 2-(5-((3-bromophenyl)thio)-1,3,4-oxadiazole-2-yl)quinazolin-4(3H)-one (8o), 4-amino-5-((4-nitrophenyl)thio)-4H-1,2,4-triazol-3-yl)quinazolin-4(3H)-one (9m) and 2-(4-amino-5-(o-tolylthio)-4H-1,2,4-triazol-3-yl)quinazolin-4(3H)-one (9b) showed good antiproliferative activity against A549 cells with IC50 value of 3.46, 2.96, and 3.44 μM, respectively. 2-(4-Amino-5-((3,5-difluorophenyl)thio)-4H-1,2,4-triazol-3-yl)quinazolin-4(3H)-one (9h) and 4-amino-5-((4-nitrophenyl)thio)-4H-1,2,4-triazol-3-yl)quinazolin-4(3H)-one (9m) had obvious inhibitory effects on Hela cells with IC50 values of 1.76 and 3.91 μM, respectively. 2-(4-Amino-5-((2-chlorophenyl)thio)-4H-1,2,4-triazol-3-yl)quinazolin-4(3H)-one (9i) showed high inhibitory activity against MCF-7 cells with IC50 value of 2.72 μM. Meanwhile, all compounds showed lower cytotoxicity to L929 cells than positive control drug 5-FU. In development, according to the above preliminary observation, these novel quinazolinones incorporating 1,3,4-oxadiazole and 1,2,4-triazole thioether moieties could become potential molecular templates for searching new antitumor agents. [ABSTRACT FROM AUTHOR]

Objective: To make a study of the influence of the intervene of traditional Chinese medicine on cytokine level in benign prostatic hyperplasia., Method: The patients were divided into treatment group (group A), control group (group B) and healthy group (group C) with the randomly compared researching method. Group A were given Qianliening Tang (QLNT), daily potion, two times a day. Group B were given Qianliekang, three times a day, three pills each time. The course of both treatment lasted for 8 weeks., Result: Before treatment, TNF-alpha, IL-17/IL-10, IL-4 in group A and B are obviously out of order compared with group C (P < 0.01). After the treatment, TNF-alpha, IL-17 in group A decreased dramatically and IL-10, IL-4 increased, which shows great differences compared with those before treatment and with group B (P < 0.01), but still can't reach to group C level., Conclusion: Qian liening Tang can efficiently regulate the level of suppressive inflammatory cytokines and proinflammatory cytokines. It provides scientific experimental basis for clinical diagnosis and treatment of the disease.

Terrestrial transect is an important and effective method for global change study. The Northeast China Transect (NECT), which is assigned along the latitude 43 degrees 30'N in the mid-latitude of temperate zone and located at 112 degrees-130 degrees 30'E and 42-46 degrees N, is one of the fifteen global transects recognized by IGBP. It is about 1600 km in length and 300 km in width. The NECT is mainly driven by precipitation, and becomes an effective platform of global change study in China. Based on the field survey in 2001 and a simulated experiment, this paper analyzed the gradient distribution of soil nitrogen and its response to climate change along the Transect. The results indicated that soil total and available nitrogen in NECT were significantly related to longitude, with a correlation coefficient being 0.695 (P < 0.001) and 0. 636 (P < 0.001), respectively, and had a similar horizontal distribution with soil organic carbon. Soil available nitrogen content in the NECT was decreased from east to west, and could be one of factors restricting plant growth. The decreasing rate of soil total and available nitrogen from topsoil to subsoil was different with ecosystems along the NECT. Soil total and available nitrogen contents had a close linear relationship with soil pH, total and labile carbon, total and available phosphorus, total sulphur, total and available zinc, available potassium, available manganese, bulk density, water holding capacity, and total porosity. They also had a significant linear relationship with precipitation, the correlation coefficient being 0.682 (P < 0.001) and 0.688 (P < 0.001), respectively. The short-term simulated experiment showed that doubled ambient CO2 concentration and soil moisture regime had no significant effects on soil total and available nitrogen, the variation coefficients being 5.55% and 3.84%, respectively.