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5. In vitro and in vivo anticancer activity of steroid saponins of paris polyphylla var. yunnanensis

Author

Yan, L.L, Zhang, Y.J., Gao, W.Y., Man, S.L., and Wang, Y.

Subjects
Original contributions
Abstract

Aim: To confirm the anticancer activity of steroid saponins isolated from the rhizome of Paris polyphylla var. yunnanensis and evaluate the structure-activity relationships of these steroid saponins in vitro and in vivo. Materials and Methods: Eight known steroid saponins were isolated from the rhizome of Paris polyphylla. The LA795 lung adenocarcinoma cell line from mice was chosen to evaluate cytotoxicity by means of MTT assay, and to study apoptosis by means of AnnexinV-FITC/PI flow cytometry. Diosgenin-3-α-L-arabinofuranosyl(1→4)-[α-L-rhamnopyranosyl(1→2)]-β-D-glycopyranoside (compound 1), the main steroid saponin of Paris polyphylla, and diosgenin (Dio), the aglycone of major steroid saponins, were evaluated for antitumor activity on LA795 lung adenocarcinoma in T739 inbred mice. Results: The steroid saponins showed remarkable cytotoxicity and caused typical apoptosis in a dose-dependent manner. They were evaluated in vivo by their effect on tumor developed in T739 inbred mice. The oral administration to T739 mice bearing LA795 lung adenocarcinoma of compound 1 and diosgenin significantly inhibited tumor growth, by 29.44% and 33.94%, respectively. HE staining showed that lungs and livers of treated mice underwent various levels of histopathological alterations. It was demonstrated by TUNEL assay that apoptosis rate in tumor cells was increased in comparison to cells in control mice. The 3-O-glycoside moiety and spirostanol structure played an important role in the anticancer activity of steroid saponins, and the number and the variety of glycosides of compounds strongly influenced on their anticancer activity. Conclusion: Rhizoma Paridis saponins showed anticancer activity against lung adenocarcinoma cell line, both in vitro and in vivo, and their effect was dependent on compounds’ structure in a certain degree.

Published
2009

7. Research on reducing feed to reverse flotation in Diaojuntai concentrator.

Author

Gao W.Y., XXIV International mineral processing congress Beijing 24-Sep-0828-Sep-08 176, Gao W.Y., and XXIV International mineral processing congress Beijing 24-Sep-0828-Sep-08 176

Abstract

The Diaojuntai concentrator employs low- and high-intensity magnetic separation followed by anionic reverse flotation on all the iron (magnetite) concentrate from magnetic separation, but reagent and energy costs are high for the flotation stage. Tests on reducing the quantity of feed to the reverse flotation resulted in a scheme where the concentrate of low-intensity magnetic separation, forming 50% of the total concentrate, was screened at 0.076 mm and the undersize fraction was treated in a concentrator with a gravity and magnetic field. Results showed that a satisfactory concentrate, with grade more than 68% Fe, could be obtained at particular velocities of rising water flow, pulsating electric currents and power on-off durations. The grade of the low-intensity magnetic concentrate was 57.32%. Using this method, a reduction of 35% overall in the volume of feed to the reverse flotation stage was achieved. Cost savings are given., The Diaojuntai concentrator employs low- and high-intensity magnetic separation followed by anionic reverse flotation on all the iron (magnetite) concentrate from magnetic separation, but reagent and energy costs are high for the flotation stage. Tests on reducing the quantity of feed to the reverse flotation resulted in a scheme where the concentrate of low-intensity magnetic separation, forming 50% of the total concentrate, was screened at 0.076 mm and the undersize fraction was treated in a concentrator with a gravity and magnetic field. Results showed that a satisfactory concentrate, with grade more than 68% Fe, could be obtained at particular velocities of rising water flow, pulsating electric currents and power on-off durations. The grade of the low-intensity magnetic concentrate was 57.32%. Using this method, a reduction of 35% overall in the volume of feed to the reverse flotation stage was achieved. Cost savings are given.

Published
2008

15. Emergence of human immunodeficiency virus type 1 variants with resistance to multiple dideoxynucleosides in patients receiving therapy with dideoxynucleosides

Author

Shirasaka, T., Kavlick, M.F., Ueno, T., Gao, W.Y., Kojima, E., Alcaide, M.L., Chokekijchai, S., Roy, B.M., Arnold, E., Yarchoan, R., and Mitsuya, H.

Subjects
Drug resistance -- Research, HIV infection -- Drug therapy, Didanosine -- Physiological aspects, Zalcitabine -- Physiological aspects
Abstract

According to the authors' abstract of an article published in Proceedings of the National Academy of Sciences of the United States of America, "A set of mutations [Ala-62 --> Val(A62V), [...]

Published
1995

16. Anti-HIV-1 activity of hydroxyurea in combination with 2',3'dideoxynucleosides

Author

Gao, W.Y., Johns, D.G., and Mitsuya, H.

Subjects
Hydroxyurea -- Evaluation, HIV (Viruses), Drug therapy, Combination -- Evaluation, Didanosine -- Evaluation, Zalcitabine -- Evaluation, HIV infection -- Drug therapy
Abstract

AUTHORS: W.Y. Gao, D.G. Johns and H. Mitsuya. U.S. National Cancer Institute, Bethesda, Maryland. According to an abstract submitted by the authors to a joint meeting of the American Federation [...]

Published
1994

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