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The core is one of the core strategic resources in the exploration and development of oil and gas fields and plays an important role in the research on facies of oil and gas reservoirs， physical properties of reservoirs， and oil content. Compared with the paper data， the physical geological data of the core has the characteristics of reduction and variability， and the original appearance of the core cannot be preserved for a long time， thus reducing the utilization value of the core. Digital core technologies can collect the original data of the core in time， enrich the geological information of the core， and improve the utilization rate of core data， so it is of great significance to research digital core technologies. According to the production practice of core digitization construction in Shengli Oilfield， the concept， equipment， and technical content of core digitization were described， and many important digital core technologies such as core gamma spectrometry measurement， XRF element testing， magnetic susceptibility testing， and CT scanning were analyzed. The geological application effects of each technology were discussed. The research results show that digital core technologies have the advantages of fast testing， good continuity， large data， and high precision. They can accurately reflect the characteristics of sediment changes and provide an important geological basis for the research on sedimentary genesis， reservoir description and evaluation， material composition， and other issues of shales， carbonate， and glutenite， with a broad application prospect. Digital core data is the continuous data of core scale obtained directly from the core， which is between the original data and the result data in terms of the nature of the data， and it focuses more on the originality of the core， which is the first-hand geological data. Digital core data has unique and important application value and is an important technical means to improve the application level of the core.

The overactivation of the osteoclasts is a crucial pathological factor in the development of osteoporosis. MZF1, belonging to the scan-zinc finger family, plays a significant role in various processes associated with tumor malignant progression and acts as an essential transcription factor regulating osteoblast expression. However, the exact role of MZF1 in osteoclasts has not been determined. In this study, the purpose of our study was to elucidate the role of MZF1 in osteoclastogenesis. First, we established MZF1-deficient female mice and evaluated the femur bone phenotype by micro–computed tomography and histological staining. Our findings indicate that MZF1−/− mice exhibited a low bone mass osteoporosis phenotype. RANKL could independently induce the differentiation of RAW264.7 cells into osteoclasts, and we found that the expression level of MZF1 protein decreased gradually. Then, the CRISPR/Cas 9 gene-editing technique was used to build a RAW264.7 cell model with MZF1 knockout, and RANKL was used to independently induce MZF1−/− and wild-type cells to differentiate into mature osteoclasts. Tartrate-resistant acid phosphatase staining and F-actin fluorescence results showed that the MZF1−/− group produced more tartrate-resistant acid phosphatase–positive mature osteoclasts and larger actin rings. The expression of osteoclast-associated genes (including tartrate-resistant acid phosphatase, CTSK, c-Fos, and NFATc1) was evaluated by reverse transcription quantitative polymerase chain reaction and Western blot. The expression of key genes of osteoclast differentiation in the MZF1−/− group was significantly increased. Furthermore, we found that cell viability was increased in the early stages of RANKL-induced cell differentiation in the MZF1−/− group cells. We examined some prevalent ferroptosis markers, including malondialdehyde, glutathione, and intracellular Fe, the active form of iron in the cytoplasm during the early stages of osteoclastogenesis. The results suggest that MZF1 may be involved in osteoclast differentiation by regulating RANKL-induced ferroptosis of osteoclasts. Collectively, our findings shed light on the essential involvement of MZF1 in the regulation of osteoclastogenesis in osteoporosis and provide insights into its potential underlying mechanism. [ABSTRACT FROM AUTHOR]

Ferroptosis is a newly discovered non-apoptotic cell death whose key is lipid peroxidation. It has been reported that ferroptosis is involved in the occurrence and development of tumors and nervous system and musculoskeletal diseases. Cellular ferroptosis contributes to the imbalance of bone homeostasis and is involved in the development of osteoporosis; however, the detailed mechanism of which is still unclear though it may provide a new direction for anti-osteoporosis. The current drugs used in the treatment of osteoporosis, such as bisphosphonates and teriparatide, have many side effects, increasing people's search for natural compounds to treat osteoporosis. This review paper briefly summarizes the current research regarding the mechanisms of ferroptosis and natural anti-osteoporosis compounds targeting its pathway., (Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.)

Objective: To explore the clinical effect of unilateral biportal endoscopic-assisted transforaminal lumbar interbody fusion (UBE-TLIF) in the treatment of recurrent lumbar disc herniation (RLDH)., Methods: The clinical data of 44 patients with RLDH treated by UBE-TLIF in our hospital from August 2020 to December 2020 were analysed retrospectively. The study indicators included intraoperative blood loss, operation time, bed rest time, and hospital stay. The follow-up data included the visual analogue score (VAS) of low back pain, Japanese Orthopaedic Association score (JOA), Oswestry disability index (ODI) score, and the short form 36 health survey questionnaire (SF-36) score preoperatively and 1 week and 6 months postoperatively., Results: The average operation time was 179.15 ± 42.06 minutes, the average intraoperative blood loss was 132.67 ± 41.92 ml, the average bed rest time was 1.51 ± 0.42 days, and the average hospital stay was 4.82 ± 1.13 days. The VAS score of low back pain after the operation was lower than that before the operation (all P<0.0001). The ODI score, JOA score, and SF-36 scores at postoperative follow-up were significantly different from those before the operation (P<0.05). The satisfaction rate was 86.4% at 7 days after the operation and 95.4% at 6 months after the operation. The proportion of significant clinical efficacy was 18.2% (postoperative day 7) and 63.6% (postoperative month 6)., Conclusions: UBE-TLIF has the advantages of a rapid recovery, less intraoperative blood loss, a short bed rest and hospital stay, and a good medium-term clinical effect. It is a safe, reliable minimally invasive technique for surgical treatment of RLDH., Competing Interests: None., (AJTR Copyright © 2022.)