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40 results on '"Gao, Zhaoming"'

11. Discovery of Prevalent Ciliophora, Discoba, and Copepoda Protists in Deep Sea Water by In Situ Nucleotide Extraction.

Author

Xu, Peikuan, Yang, Ming, He, Lisheng, Zhang, Hongxi, Gao, Zhaoming, Jiang, Yuelu, and Wang, Yong

Subjects
SEAWATER, COPEPODA, PROTISTA, DETECTION of microorganisms, HYDROSTATIC pressure, CILIATA, DEEP-sea animals, SUBMARINE fans
Abstract

Deep-sea eukaryotic microorganisms play a vital role in degrading organic matter and geochemically cycling elements in the deep ocean. However, the impact of sampling methods on detection of these microorganisms under high hydrostatic pressure remains uncertain. In this study, we compared a traditional water sampling method using a Niskin bottle, an in situ microbial filtration and fixation method (ISMIFF), and a multiple in situ nucleic acid collection (MISNAC) method to exhibit differences in the community structures that were collected at ~590–3100 m in the South China Sea (SCS). The classification and biodiversity indices of 18S rDNA Illumina sequencing reads from the V9 variation region revealed higher diversity for MISNAC DNA absorption column samples compared to others. Importantly, the relative abundance of Ciliophora (19.49%), Copepoda (23.31%), and Diplonemea (10.67%) was higher in MISNAC adsorption column samples, while Retaria (48.86%) were dominant in the MISNAC membrane samples. This indicates that MISNAC columns might collect more DNA in situ for the naked protists, while Retaria with a carbonate shell were more likely retained on the membrane. In conclusion, MISNAC is an effective method for DNA collection of deep-sea eukaryotic microorganisms and provides valuable materials for studying deep-sea microbial ecosystems. [ABSTRACT FROM AUTHOR]

Published
2024
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12. Morphology and genome of a snailfish from the Mariana Trench provide insights into deep-sea adaptation

Author

Wang, Kun, Shen, Yanjun, Yang, Yongzhi, Gan, Xiaoni, Liu, Guichun, Hu, Kuang, Li, Yongxin, Gao, Zhaoming, Zhu, Li, Yan, Guoyong, He, Lisheng, Shan, Xiujuan, Yang, Liandong, Lu, Suxiang, Zeng, Honghui, Pan, Xiangyu, Liu, Chang, Yuan, Yuan, Feng, Chenguang, Xu, Wenjie, Zhu, Chenglong, Xiao, Wuhan, Dong, Yang, Wang, Wen, Qiu, Qiang, and He, Shunping

Published
2019
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16. A Study on the Optimization of Wind Environment of Existing Villa Buildings in Lingnan Area: A Case Study of Jiangmen’s “Yunshan Poetic” Moon Island Houses

Author

Gao, Zhaoming Du, Weihong Guo, Weicong Li, and Xuyi

Subjects
humid and hot area, villa building, wind environment, design strategy
Abstract

Effective natural ventilation reduces humidity, cools the space, and enhances thermal comfort. In light of the frequent ventilation issues in the Lingnan area, this research suggests a successful ventilation technique using Jiangmen’s “Yunshan Poetic” Moon Island houses as an example. With its symmetrical architectural layout of townhouses and its primary courtyard villa product, the community typifies the Lingnan area. First off, we discovered that the district’s average temperature is as high as 30.95 °C and its average humidity is as high as 83.592%RH using actual measurements and simulation of heat and humidity data. The district’s buildings’ issues with dampness, peeling walls, and substance mold are primarily caused by poor ventilation. Secondly, the PHOENICS program was used to provide efficient ventilation solutions for the following six aspects: external wind infusion organization, group orientation layout, planar grouping optimization, building façade combination, monolithic building openings, and indoor ventilation block. In order to determine if the technique is effective, the ventilation variables are compared before and after optimization using the Building Ventilation Effectiveness Test and Evaluation Criteria. The study concluded that the building’s architectural characteristics and the local climate have an impact on natural ventilation’s effectiveness. This serves as a guide for both the scientific layout development of future urban settlements and the optimization of ventilation of existing villa buildings in humid and hot areas.

Published
2022
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25. Potential sexual pheromones of sea cucumber Apostichopus japonicusand their functions on aggregative and spawning behavior

Author

Wang, Jiaze, Liang, Wenke, Gao, Zhaoming, Zhang, Libin, Deng, Beini, Chen, Xinhui, Sha, Zhenxia, and Ru, Xiaoshang

Abstract

The surging market demand for sea cucumbers (Apostichopus japonicus) has fueled a thriving growth in the sea cucumber breeding industry. Pheromones are molecules that evolve signals in defined proportions in the case of multicomponent pheromones, emitted by one individual and received by a second individual of the same species to cause a specific response. It remains an enigma as to how the species of sea cucumber, devoid of visual, auditory, and olfactory senses, aggregate and communicate within their groups, as well as coordinate gamete release during the breeding period. We hypothesize that pheromones may serve as the medium for facilitating these behaviors and propose conducting experiments centered around pheromonal investigation. In this study, we aimed to analyze common metabolites of male A. japonicususing LC-MS/MS technology and bioinformatics. Coelomic fluid and ejaculate fluid were analyzed, with aquaculture water serving as the control. Eight target pheromones were analyzed, and eight of metabolite analogs were tested for aggregation and spawning behaviors. Results showed that β-sitosterol at a concentration of 10 μg·L−1significantly promoted aggregation (21.25 % average rate) and induced a significant increase in spawning rate (58 %) of A. japonicus. The significance of this study lies in the fact that by collecting and utilizing pheromones of A. japonicus, farmers can accurately determine the reproductive cycle and optimal breeding season for A. japonicus. This enables fish farmers to implement effective breeding management strategies at the appropriate time, thereby increasing the success rate of reproduction and providing valuable technical support for the cultivation of A. japonicusseedlings.

Published
2024
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28. 第二肉身：数据时代的隐私与隐私危机

Author

Gao, Zhaoming, Gao, Hao, and Integrated Circuits

Subjects
Data_MISCELLANEOUS, ComputingMilieux_COMPUTERSANDSOCIETY
Abstract

Data are the second human flesh. The core of privacy in the data age is data privacy, and the core of privacy crisis is data privacy crisis. People are both plural and singular. Without privacy, there is no humanity, personality and dignity. Social members are generally divided into exposed and naked viewers and hidden viewers due to their privacy. Data monopoly can become a way of social control and slavery. The data privacy crisis is fundamentally an existential crisis of contemporary mankind.

Published
2019

29. A novel bacterial phylum that participates in carbon and osmolyte cycling in the Challenger Deep sediments.

Author

Cui, Guojie, Zhou, Yingli, Li, Wenli, Gao, Zhaoming, Huang, Jiaomei, and Wang, Yong

Subjects
CARBON cycle, CYTOCHROME oxidase, CYCLING competitions, SEDIMENTS, CARBON metabolism, EXTREME environments
Abstract

Summary: Large amounts of detrital organic matter and osmolytes accumulate in the sediments of hadal trenches (>6000 m depth) due to the funnelling effect. It is still unknown whether there are novel active microbes that depend on specific carbon sources in extreme and isolated environments. In this study, we present a novel active bacterial phylum, Candidatus Tianyabacteria in the FCB superphylum, which was enriched in sediments collected from the Challenger Deep. Genome binning resulted in high‐quality Ca. Tianyabacteria genomes representing two Ca. Tianyabacteria lineages (L1 and L2) in sediments 0–21 cm below the surface (cmbsf); L1 tends to be abundant in the upper layers (0–9 cmbsf), and L2 seems to be more prevalent in the deeper layers (12–21 cmbsf). Gene annotation and transcriptomics results indicate that the two lineages might import and catalyse amino acids and myo‐inositol into central carbon metabolism for a heterotrophic lifestyle. Probably due to differences in environmental oxygen levels, the L2 genomes harbour gene clusters responsible for denitrification and fermentation, while the L1 genomes encode octahaem cytochrome c and multicopper oxidase using unknown substrates. The Ca. Tianyabacteria are thus novel heterotrophic organisms that participate in processes of carbon, nitrogen and organic osmolyte cycling in hadal sediments. [ABSTRACT FROM AUTHOR]

Published
2021
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