12 results on '"García-Nieto, Daniel"'
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2. Electrochemiluminescent nanostructured DNA biosensor for SARS-CoV-2 detection
- Author
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Gutiérrez-Gálvez, Laura, del Caño, Rafael, Menéndez-Luque, Iris, García-Nieto, Daniel, Rodríguez-Peña, Micaela, Luna, Mónica, Pineda, Teresa, Pariente, Félix, García-Mendiola, Tania, and Lorenzo, Encarnación
- Published
- 2022
- Full Text
- View/download PDF
3. Amplification-free detection of SARS-CoV-2 using gold nanotriangles functionalized with oligonucleotides
- Author
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del Caño, Rafael, García-Mendiola, Tania, García-Nieto, Daniel, Álvaro, Raquel, Luna, Mónica, Iniesta, Hernán Alarcón, Coloma, Rocío, Diaz, Ciro Rodríguez, Milán-Rois, Paula, Castellanos, Milagros, Abreu, Melanie, Cantón, Rafael, Galán, Juan Carlos, Pineda, Teresa, Pariente, Félix, Miranda, Rodolfo, Somoza, Álvaro, and Lorenzo, Encarnación
- Published
- 2022
- Full Text
- View/download PDF
4. Electrochemiluminescent nanostructured DNA biosensor for SARS-CoV-2 detection
- Author
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Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Ministerio de Economía y Competitividad (España), Fundación Instituto Madrileño de Estudios Avanzados en Nanociencia, Banco Santander, Universidad Autónoma de Madrid, Conferencia de Rectores de las Universidades Españolas, Consejo Superior de Investigaciones Científicas (España), Caño, Rafael del [0000-0001-8762-8455], García-Nieto, Daniel [0000-0002-3006-0114], Luna, Mónica [0000-0002-7104-0726], García-Mendiola, Tania [0000-0002-7634-5844], Gutiérrez-Gálvez, Laura, Caño, Rafael del, Menéndez-Luque, Iris, García-Nieto, Daniel, Rodríguez-Peña, Micaela, Luna, Mónica, Pineda, Teresa, Pariente, Félix, García-Mendiola, Tania, Lorenzo, Encarnación, Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Ministerio de Economía y Competitividad (España), Fundación Instituto Madrileño de Estudios Avanzados en Nanociencia, Banco Santander, Universidad Autónoma de Madrid, Conferencia de Rectores de las Universidades Españolas, Consejo Superior de Investigaciones Científicas (España), Caño, Rafael del [0000-0001-8762-8455], García-Nieto, Daniel [0000-0002-3006-0114], Luna, Mónica [0000-0002-7104-0726], García-Mendiola, Tania [0000-0002-7634-5844], Gutiérrez-Gálvez, Laura, Caño, Rafael del, Menéndez-Luque, Iris, García-Nieto, Daniel, Rodríguez-Peña, Micaela, Luna, Mónica, Pineda, Teresa, Pariente, Félix, García-Mendiola, Tania, and Lorenzo, Encarnación
- Abstract
This work focuses on the development of an electrochemiluminescent nanostructured DNA biosensor for SARS-CoV-2 detection. Gold nanomaterials (AuNMs), specifically, a mixture of gold nanotriangles (AuNTs) and gold nanoparticles (AuNPs), are used to modified disposable electrodes that serve as an improved nanostructured electrochemiluminescent platform for DNA detection. Carbon nanodots (CDs), prepared by green chemistry, are used as coreactants agents in the [Ru(bpy)3]2+ anodic electrochemiluminescence (ECL) and the hybridization is detected by changes in the ECL signal of [Ru(bpy)3]2+/CDs in combination with AuNMs nanostructures. The biosensor is shown to detect a DNA sequence corresponding to SARS-CoV-2 with a detection limit of 514 aM.
- Published
- 2022
5. MoS2-Carbon Nanodots as a New Electrochemiluminescence Platform for Breast Cancer Biomarker Detection
- Author
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Gutiérrez-Gálvez, Laura, primary, Sulleiro, Manuel Vázquez, additional, Gutiérrez-Sánchez, Cristina, additional, García-Nieto, Daniel, additional, Luna, Mónica, additional, Pérez, Emilio M., additional, García-Mendiola, Tania, additional, and Lorenzo, Encarnación, additional
- Published
- 2023
- Full Text
- View/download PDF
6. Supplementary Material MoS2-Carbon Nanodots as New Electrochemiluminescence Platform for Breast Cancer Biomarker Detection
- Author
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Gutiérrez-Gálvez, Laura, Vázquez Sulleiro, Manuel, Gutiérrez-Sánchez, Cristina, García-Nieto, Daniel, Luna, Mónica, Pérez, Emilio M., García-Mendiola, Tania, Lorenzo, Encarnación, Gutiérrez-Gálvez, Laura, Vázquez Sulleiro, Manuel, Gutiérrez-Sánchez, Cristina, García-Nieto, Daniel, Luna, Mónica, Pérez, Emilio M., García-Mendiola, Tania, and Lorenzo, Encarnación
- Published
- 2023
7. MoS2-Carbon nanodots as a new electrochemiluminescence platform for breast cancer biomarker detection
- Author
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Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Ministerio de Economía y Competitividad (España), Comunidad de Madrid, Consejo Superior de Investigaciones Científicas (España), European Commission, Ministerio de Ciencia e Innovación (España), Gutiérrez-Gálvez, Laura, Vázquez Sulleiro, Manuel, Gutiérrez-Sánchez, Cristina, García-Nieto, Daniel, Luna, Mónica, Pérez, Emilio M., García-Mendiola, Tania, Lorenzo, Encarnación, Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Ministerio de Economía y Competitividad (España), Comunidad de Madrid, Consejo Superior de Investigaciones Científicas (España), European Commission, Ministerio de Ciencia e Innovación (España), Gutiérrez-Gálvez, Laura, Vázquez Sulleiro, Manuel, Gutiérrez-Sánchez, Cristina, García-Nieto, Daniel, Luna, Mónica, Pérez, Emilio M., García-Mendiola, Tania, and Lorenzo, Encarnación
- Abstract
In this work, we present the combination of two different types of nanomaterials, 2D molybdenum disulfide nanosheets (MoS2-NS) and zero-dimensional carbon nanodots (CDs), for the development of a new electrochemiluminescence (ECL) platform for the early detection and quantification of the biomarker human epidermal growth factor receptor 2 (HER2), whose overexpression is associated with breast cancer. MoS2-NS are used as an immobilization platform for the thiolated aptamer, which can recognize the HER2 epitope peptide with high affinity, and CDs act as coreactants of the anodic oxidation of the luminophore [Ru(bpy)3]2+. The HER2 biomarker is detected by changes in the ECL signal of the [Ru(bpy)3]2+/CD system, with a low detection limit of 1.84 fg/mL and a wide linear range. The proposed method has been successfully applied to detect the HER2 biomarker in human serum samples.
- Published
- 2023
8. Electronic Supplementary Material Amplification-Free Detection of SARS-CoV-2 using Gold Nanotriangles Functionalized with Oligonucleotides
- Author
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Caño, Rafael del, García-Mendiola, Tania, García-Nieto, Daniel, Álvaro Bruna, Raquel, Luna, Mónica, Alarcón Iniesta, Hernán, Coloma, Rocío, Rodríguez Diaz, Ciro, Milán-Rois, Paula, Castellanos, Milagros, Abreu, Melanie, Cantón, Rafael, Galán, Juan Carlos, Pineda, Teresa, Pariente, Félix, Miranda, Rodolfo, Somoza, Álvaro, Lorenzo, Encarnación, Caño, Rafael del, García-Mendiola, Tania, García-Nieto, Daniel, Álvaro Bruna, Raquel, Luna, Mónica, Alarcón Iniesta, Hernán, Coloma, Rocío, Rodríguez Diaz, Ciro, Milán-Rois, Paula, Castellanos, Milagros, Abreu, Melanie, Cantón, Rafael, Galán, Juan Carlos, Pineda, Teresa, Pariente, Félix, Miranda, Rodolfo, Somoza, Álvaro, and Lorenzo, Encarnación
- Published
- 2022
9. Methylene Blue functionalized carbon nanodots combined with different shape gold nanostructures for sensitive and selective SARS-CoV-2 sensing
- Author
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Comunidad de Madrid, Ministerio de Economía y Competitividad (España), Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Conferencia de Rectores de las Universidades Españolas, Banco Santander, Consejo Superior de Investigaciones Científicas (España), Pina-Coronado, Clara, Martínez-Sobrino, Álvaro, Gutiérrez-Gálvez, Laura, Caño, Rafael del, Martínez-Periñán, Emiliano, García-Nieto, Daniel, Rodríguez-Peña, Micaela, Luna, Mónica, Milán-Rois, Paula, Castellanos, Milagros, Abreu, Melanie, Cantón, Rafael, Galán, Juan Carlos, Pineda, Teresa, Pariente, Félix, Somoza, Álvaro, García-Mendiola, Tania, Miranda, Rodolfo, Lorenzo, Encarnación, Comunidad de Madrid, Ministerio de Economía y Competitividad (España), Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Conferencia de Rectores de las Universidades Españolas, Banco Santander, Consejo Superior de Investigaciones Científicas (España), Pina-Coronado, Clara, Martínez-Sobrino, Álvaro, Gutiérrez-Gálvez, Laura, Caño, Rafael del, Martínez-Periñán, Emiliano, García-Nieto, Daniel, Rodríguez-Peña, Micaela, Luna, Mónica, Milán-Rois, Paula, Castellanos, Milagros, Abreu, Melanie, Cantón, Rafael, Galán, Juan Carlos, Pineda, Teresa, Pariente, Félix, Somoza, Álvaro, García-Mendiola, Tania, Miranda, Rodolfo, and Lorenzo, Encarnación
- Abstract
The development of DNA-sensing platforms based on new synthetized Methylene Blue functionalized carbon nanodots combined with different shape gold nanostructures (AuNs), as a new pathway to develop a selective and sensitive methodology for SARS-CoV-2 detection is presented. A mixture of gold nanoparticles and gold nanotriangles have been synthetized to modify disposable electrodes that act as an enhanced nanostructured electrochemical surface for DNA probe immobilization. On the other hand, modified carbon nanodots prepared a la carte to contain Methylene Blue (MB-CDs) are used as electrochemical indicators of the hybridization event. These MB-CDs, due to their structure, are able to interact differently with double and single-stranded DNA molecules. Based on this strategy, target sequences of the SARS-CoV-2 virus have been detected in a straightforward way and rapidly with a detection limit of 2.00 aM. Moreover, this platform allows the detection of the SARS-CoV-2 sequence in the presence of other viruses, and also a single nucleotide polymorphism (SNPs). The developed approach has been tested directly on RNA obtained from nasopharyngeal samples from COVID-19 patients, avoiding any amplification process. The results agree well with those obtained by RT-qPCR or reverse transcription quantitative polymerase chain reaction technique.
- Published
- 2022
10. Amplification-free detection of SARS-CoV-2 using gold nanotriangles functionalized with oligonucleotides
- Author
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Consejo Superior de Investigaciones Científicas (España), Conferencia de Rectores de las Universidades Españolas, Comunidad de Madrid, Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Ministerio de Economía y Competitividad (España), European Commission, Banco Santander, Instituto de Salud Carlos III, Caño, Rafael del, García-Mendiola, Tania, García-Nieto, Daniel, Álvaro Bruna, Raquel, Luna, Mónica, Alarcón Iniesta, Hernán, Coloma, Rocío, Rodríguez Diaz, Ciro, Milán-Rois, Paula, Castellanos, Milagros, Abreu, Melanie, Cantón, Rafael, Galán, Juan Carlos, Pineda, Teresa, Pariente, Félix, Miranda, Rodolfo, Somoza, Álvaro, Lorenzo, Encarnación, Consejo Superior de Investigaciones Científicas (España), Conferencia de Rectores de las Universidades Españolas, Comunidad de Madrid, Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Ministerio de Economía y Competitividad (España), European Commission, Banco Santander, Instituto de Salud Carlos III, Caño, Rafael del, García-Mendiola, Tania, García-Nieto, Daniel, Álvaro Bruna, Raquel, Luna, Mónica, Alarcón Iniesta, Hernán, Coloma, Rocío, Rodríguez Diaz, Ciro, Milán-Rois, Paula, Castellanos, Milagros, Abreu, Melanie, Cantón, Rafael, Galán, Juan Carlos, Pineda, Teresa, Pariente, Félix, Miranda, Rodolfo, Somoza, Álvaro, and Lorenzo, Encarnación
- Abstract
Gold nanotriangles (AuNTs) functionalized with dithiolated oligonucleotides have been employed to develop an amplification-free electrochemical biosensor for SARS-CoV-2 in patient samples. Gold nanotriangles, prepared through a seed-mediated growth method and exhaustively characterized by different techniques, serve as an improved electrochemical platform and for DNA probe immobilization. Azure A is used as an electrochemical indicator of the hybridization event. The biosensor detects either single stranded DNA or RNA sequences of SARS-CoV-2 of different lengths, with a low detection limit of 22.2 fM. In addition, it allows to detect point mutations in SARS-CoV-2 genome with the aim to detect more infective SARS-CoV-2 variants such as Alpha, Beta, Gamma, Delta, and Omicron. Results obtained with the biosensor in nasopharyngeal swab samples from COVID-19 patients show the possibility to clearly discriminate between non-infected and infected patient samples as well as patient samples with different viral load. Furthermore, the results correlate well with those obtained by the gold standard technique RT-qPCR, with the advantage of avoiding the amplification process and the need of sophisticated equipment.
- Published
- 2022
11. MoS 2 -Carbon Nanodots as a New Electrochemiluminescence Platform for Breast Cancer Biomarker Detection.
- Author
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Gutiérrez-Gálvez, Laura, Sulleiro, Manuel Vázquez, Gutiérrez-Sánchez, Cristina, García-Nieto, Daniel, Luna, Mónica, Pérez, Emilio M., García-Mendiola, Tania, and Lorenzo, Encarnación
- Subjects
ELECTROCHEMILUMINESCENCE ,APTAMERS ,EPIDERMAL growth factor receptors ,EARLY detection of cancer ,BREAST cancer ,MOLYBDENUM disulfide - Abstract
In this work, we present the combination of two different types of nanomaterials, 2D molybdenum disulfide nanosheets (MoS
2 -NS) and zero-dimensional carbon nanodots (CDs), for the development of a new electrochemiluminescence (ECL) platform for the early detection and quantification of the biomarker human epidermal growth factor receptor 2 (HER2), whose overexpression is associated with breast cancer. MoS2 -NS are used as an immobilization platform for the thiolated aptamer, which can recognize the HER2 epitope peptide with high affinity, and CDs act as coreactants of the anodic oxidation of the luminophore [Ru(bpy)3 ]2+ . The HER2 biomarker is detected by changes in the ECL signal of the [Ru(bpy)3 ]2+ /CD system, with a low detection limit of 1.84 fg/mL and a wide linear range. The proposed method has been successfully applied to detect the HER2 biomarker in human serum samples. [ABSTRACT FROM AUTHOR]- Published
- 2023
- Full Text
- View/download PDF
12. Electrochemiluminescent nanostructured DNA biosensor for SARS-CoV-2 detection
- Author
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Laura Gutiérrez-Gálvez, Rafael del Caño, Iris Menéndez-Luque, Daniel García-Nieto, Micaela Rodríguez-Peña, Mónica Luna, Teresa Pineda, Félix Pariente, Tania García-Mendiola, Encarnación Lorenzo, Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Ministerio de Economía y Competitividad (España), Fundación Instituto Madrileño de Estudios Avanzados en Nanociencia, Banco Santander, Universidad Autónoma de Madrid, Conferencia de Rectores de las Universidades Españolas, Consejo Superior de Investigaciones Científicas (España), Caño, Rafael del, García-Nieto, Daniel, Luna, Mónica, García-Mendiola, Tania, Caño, Rafael del [0000-0001-8762-8455], García-Nieto, Daniel [0000-0002-3006-0114], Luna, Mónica [0000-0002-7104-0726], and García-Mendiola, Tania [0000-0002-7634-5844]
- Subjects
SARS-CoV-2 ,COVID-19 ,Metal Nanoparticles ,Biosensing Techniques ,DNA ,Electrochemical Techniques ,Article ,Analytical Chemistry ,Nanostructures ,AuNMs ,Luminescent Measurements ,Humans ,Gold ,DNA biosensor - Abstract
This work focuses on the development of an electrochemiluminescent nanostructured DNA biosensor for SARS-CoV-2 detection. Gold nanomaterials (AuNMs), specifically, a mixture of gold nanotriangles (AuNTs) and gold nanoparticles (AuNPs), are used to modified disposable electrodes that serve as an improved nanostructured electrochemiluminescent platform for DNA detection. Carbon nanodots (CDs), prepared by green chemistry, are used as coreactants agents in the [Ru(bpy)3]2+ anodic electrochemiluminescence (ECL) and the hybridization is detected by changes in the ECL signal of [Ru(bpy)3]2+/CDs in combination with AuNMs nanostructures. The biosensor is shown to detect a DNA sequence corresponding to SARS-CoV-2 with a detection limit of 514 aM., This work has been financially supported by the Spanish Ministry of Economy and Competitiveness (PID2020-116728RB-I00, CTQ2015-71955-REDT (ELECTROBIONET)) and Community of Madrid (TRANSNANOAVANSENS, S2018/NMT-4342). RdC gratefully thanks support from Fundación IMDEA, UAM and Banco Santander (fondo supera 2020, convocatoria CRUE–CSIC–SANTANDER, project with reference 10.01.03.02.41).
- Published
- 2022
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