Search

Author(s): Luke R. Thompson [1, 2, 3]; Jon G. Sanders [1]; Daniel McDonald [1]; Amnon Amir [1]; Joshua Ladau [4]; Kenneth J. Locey [5]; Robert J. Prill [6]; Anupriya Tripathi [...], Our growing awareness of the microbial worlds importance and diversity contrasts starkly with our limited understanding of its fundamental structure. Despite recent advances in DNA sequencing, a lack of standardized protocols and common analytical frameworks impedes comparisons among studies, hindering the development of global inferences about microbial life on Earth. Here we present a meta-analysis of microbial community samples collected by hundreds of researchers for the Earth Microbiome Project. Coordinated protocols and new analytical methods, particularly the use of exact sequences instead of clustered operational taxonomic units, enable bacterial and archaeal ribosomal RNA gene sequences to be followed across multiple studies and allow us to explore patterns of diversity at an unprecedented scale. The result is both a reference database giving global context to DNA sequence data and a framework for incorporating data from future studies, fostering increasingly complete characterization of Earths microbial diversity.

Stable isotopes are useful for estimating livestock diet selection. The objective was to compare δ13C and δ15N to estimate diet proportion of C3–C4 forages when steers (Bos spp.) were fed quantities of rhizoma peanut (Arachisglabrata; RP; C3) and bahiagrass (Paspalumnotatum; C4).Treatments were proportions of RP with bahiagrass hay: 100% bahiagrass (0%RP); 25% RP + 75% bahiagrass (25%RP); 50% RP + 50% bahiagrass (50%RP); 75% RP + 25% bahiagrass (75%RP); and 100% RP (100% RP). Feces, plasma, red blood cell (RBC), and hair were collected at 8-days intervals, for 32 days. Two-pool mixing model was utilized to back-calculate the proportion of RP based on the sample and forage δ13C or δ15N. Feces showed changes using δ13C by 8 days, and adj. R2 between predicted and observed RP proportion was 0.81 by 8 days. Plasma, hair, and RBC required beyond 32-days to reach equilibrium, therefore were not useful predictors of diet composition during the study. Diets were best represented using fecal δ13C at both 8-days and 32-days. By 32-days, fecal δ15N showed promise (R2 = 0.71) for predicting diet composition in C3–C4 diets. Further studies are warranted to further corroborate fecal δ15N as a predictor of diet composition in cattle. [ABSTRACT FROM AUTHOR]

As the demand of nutrients by the population will continue to grow over the years, efficient practices are required in the food chain to satisfy the demand. This practice involves the intensification of production systems. Moreover, intensification can potentially harm the animal or environment if the resources are used to the limit. The latter, product of pushing to the limit the ruminal environment. Dietary buffers and tannins offer a key role addressing issues related to intensification. This study aimed to evaluate the effects of two dietary buffers and tannins. Ruminal fermentation parameters, in vitro methane production, ruminal pH, in vitro organic matter digestibility were measured. Eight ruminally cannulated steers were used in a duplicated 4 x 4 Latin square design. Steers were fed with a basal diet comprised of 35.5% corn silage, 18.7% protein supplement, 21.5% corn grain, 22% bermuda hay, 1% mineral supplement (DM basis). Four treatments were used: BICARB (Basal diet + 1.2% DM Sodium Bicarbonate), BRIC-050 (Basal diet + 1.2% DM BRIC), BRIC-150 (Basal diet + 1.2% DM BRIC + 0.15 % DM Tannins), TAN (Basal diet + 0.15% DM Tannins). Steers were housed in individual pens and received every morning a TMR and water ad libitum. The experiment consisted of 4 periods with 16 days total, composed by 3 d of covariates, 7 d of treatment adaptation, 1 d of 50% feed restriction, 1 d SARA challenge, 4 d of recovery, and 20 d of washout between periods. Two in vitro batch culture were performed in every period at the last day of covariates and last day of recovery, collecting ruminal fluid from each animal. Treatments with BICARB promoted a higher pH than TAN during the adaptation at 4 h after feeding (P = 0.01). Moreover, BRIC-150 showed similar results with a tendency to increase (P = 0.07) when compared with TAN. During the feed restriction BRIC-150 showed a greater pH (P = 0.01) when compared with TAN. While, for BICARB and BRIC-050 pH tended to be greater (P = 0.06) than TAN. During the SARA challenge and the recovery, treatments with buffer tended to have greater pH (P < 0.1). A significant reduction ammonia nitrogen was observed for BICARB and TAN compared with BRIC-050 and BRIC-150 (P < 0.01). Overall, the three buffers tested showed similar results in terms of maintaining a greater pH than treatment without buffer. [ABSTRACT FROM AUTHOR]

Stockpiling limpograss [Hemarthria altissima (Poir.) Stapf & C.E. Hubb.] may be an option to extend the grazing season and decrease the needs for conserved forage in Florida. This 2-yr study evaluated herbage accumulation (HA), in vitro digestible organic matter (IVDOM), and crude protein (CP) concentrations of four limpograss entries (‘Floralta’, ‘Gibtuck’, ‘Kenhy’, and Entry 1) and ‘Tifton 85′ bermudagrass [Cynodon spp.] during the growth (May–August) and stockpiling (September– January) periods. During the growth period, treatments were the five grasses. During the stockpiling period, treatments were allocated in a split-plot arrangement with five grasses (main plot) and four stockpiling periods (5, 10, 15, or 20 wk; subplot). Limpograss and Tifton 85 had similar total HA (mean = 8,150 lb drymatter [DM] acre–1) during the growth period, and Gibtuck had greater HA per harvest than Kenhy and Entry 1 (2,360 vs. 1,940 lb DM acre–1, respectively). Regardless of the stockpiling period, HA was similar among treatments. At 15 wk, HA was maximized in both years, despite being similar to 10 and 20 wk in the first year. In general, limpograss had greater IVDOM and lesser CP concentration than Tifton 85 during growth and stockpiling periods. The decline in IVDOM was slower for stockpiled limpograss than Tifton 85, with greater differences occurring at 15 and 20 wk. Stockpiling limpograss may be an alternative to extend the grazing season in livestock operations, however protein supplementation might be needed to maintain or improve animal performance. [ABSTRACT FROM AUTHOR]

Nitrogen fertilization is a common practice for sustaining forage production in forage systems in southeastern United States. Warm‐season annual legumes may be an alternative forage to warm‐season perennial grasses that do not require N fertilization. Sunn hemp (Crotalaria juncea L.) is a fast‐growing, warm‐season annual legume native to India and Pakistan. The objective of this 2‐year study was to assess the herbage accumulation (HA), atmospheric N2 fixation (ANF) and nutritive value of sunn hemp. Treatments were the factorial arrangement of two sunn hemp cultivars ("Crescent Sun" and "Blue Leaf"), three seeding rates (17, 28 and 39 kg seed/ha) and seed inoculation (inoculated or non‐inoculated seeds), distributed in a randomized complete block design with four replicates. Crescent sun had greater HA (3,218 vs. 1764 kg DM/ha) and ANF (41 vs. 25 kg N/ha). Blue leaf had greater crude protein (CP) (188 vs. 176 g/kg) and in vitro digestible organic matter (IVDOM) concentrations (564 vs. 531 g/kg) than crescent sun. Non‐inoculated seed had greater CP than inoculated seed, 188 and 177 g/kg, respectively, and inoculation did not affect HA. Intermediate seeding rate (28 kg/ha) decreased HA (2002 kg DM/ha), while HA from high and low seeding rates (17 and 39 kg/ha, respectively) did not differ (2,863 and 2,615 kg DM/ha respectively). Planting non‐inoculated crescent sun at 17 kg/ha seeding rate is a feasible management practice to produce sunn hemp in subtropical regions; however, inoculation should always be recommended for proper establishment. [ABSTRACT FROM AUTHOR]

The search for alternatives to mitigate enteric methane emissions remains a top priority to ensure the continued sustainability of the livestock industry. Exploration of new direct-fed microbials with potential in ruminant nutrition is an active and promising area of research. This study aimed to evaluate the effects of feeding a novel probiotic on ruminal fermentation parameters, in vitro methane production, and nutrient utilization in beef steers. A total of 12 ruminally cannulated American Aberdeen steers (348 ± 37 kg BW) were used in a 4 × 4 Latin square design, with each period lasting for 35 d. The steers were fed a basal diet comprised of 82% corn silage, 12% cotton gin trash, 5% cottonseed meal, and 1% vitamin and mineral supplement on a dry matter (DM) basis. Four treatments were used in this study: CTL (no additive), B2 (probiotic at a dosage of 2 × 109 CFU/animal/d), B10 (probiotic at a dosage of 10 × 109 CFU/animal/d), and B30 (probiotic at a dosage of 30 × 109 CFU/animal/d). Steers were housed in two pens and were sorted daily to receive the treatments individually in a carrier of corn silage (250 g/d). The experiment consisted of 4 periods, with 21 d of adaptation to the treatments, 7 d of measurements, and 7 d of washout between periods. During the study, two in vitro batch culture incubations were performed in each period collecting ruminal fluid from each animal individually (experimental unit): one on d 0 before steers received the treatments, and one after 21 d of adaptation. The incubations on day 0 were used as a baseline. The use of this probiotic did not affect (P > 0.05) in vitro ruminal fluid pH, volatile fatty acids, or acetate to propionate ratio. However, there was a cubic effect (P < 0.05) on in vitro organic matter digestibility with the inclusion of probiotic, and the greatest digestibility was observed in the B2 treatment. The concentration of ammonia nitrogen linearly increased (P < 0.05) with the inclusion of probiotic with CTL and B2 treatments showing the least values. While there was no effect (P > 0.05) on total gas production among treatments, methane production (CH4) was decreased (P < 0.05), showing a quadratic effect where the B2 treatment showed the least values. These results suggest that adding this novel probiotic to beef steers fed a corn silage-based diet at a dose of 2× 109 CFU per day (B2) may have positive results in reducing CH4 and improving digestibility. However, confirmation of these results will be required by in vivo methane emissions data. [ABSTRACT FROM AUTHOR]

Bahiagrass (Paspalum notatum Flüggé) and rhizoma peanut (RP, Arachis glabrata Benth.) are widely used in Florida, and growing them in association may decrease the need for N fertilization. This study evaluated herbage responses of mixed RP-bahiagrass swards in comparison with their monocultures. The eight treatments were two bahiagrass entries ('Argentine' and DF9, receiving 90 kg N ha-1 harvest-1) and two RP entries (Ecoturf and Q6B) in monoculture and the combinations of each bahiagrass with each RP (Argentine-Ecoturf, Argentine-Q6B, DF9-Ecoturf, and DF9-Q6B). There was no difference in total herbage accumulation (HA) in 2015. In 2016, total HA was greatest for Argentine + N (9630 kg dry matter [DM] ha-1), followed by the mixture Q6B-DF9 (5910 kg DM ha-1). Mixtures produced as much biomass as RP monocultures and DF9 + N. In mixtures, Argentine was the most competitive bahiagrass, whereas Q6B was the most competitive RP. Crude protein concentration of DF9 in the mixture with Q6B was similar to that of N-fertilized DF9. The total aboveground N was usually greatest in RP monocultures (50-53 kg N ha-1 harvest-1). Percentage of N derived from the atmosphere increased by 22% in Q6B from 2015 to 2016. Biological N2 fixation ranged from 11 (Ecoturf-Argentine) to 44 kg N ha-1 harvest-1 (Ecoturf and Q6B). Entry growth habit affected the proportion of each component in the sward, and this information is crucial when combining warm season grasses and legumes. Rhizoma peanut will add N to bahiagrass systems and improve forage nutritive value but have less HA than heavily fertilized grass. [ABSTRACT FROM AUTHOR]

Grasslands in warm-climate regions are often based on grass monocultures, increasing their dependence on N fertilizers. Integrating perennial legumes into grass pastures is a logical option. The objective of this 2-yr study was to assess seven rhizoma peanut (Arachis glabrata Benth) cultivars: Arbrook, Arblick, Ecoturf, Florigraze, Latitude 34, UF Peace, and UF Tito. Above- and belowground responses included biomass, in vitro organic matter disappearance (IVOMD), N concentration, N content, δ15N, proportion of N derived from atmosphere (%Ndfa), and biological N2 fixation (BNF). Arbrook was more productive than Florigraze in both years (P ⨚ 0.05) but produced similar biomass to other varieties in 2014. In 2015, Arbrook also was more productive than Arblick and Latitude 34. Herbage N concentration ranged from 19.2 to 36.3 g kg-1. Arbrook tended to be less digestible than other rhizoma peanut cultivars. The BNF represented >80% of herbage N and averaged 200 kg N ha-1 yr-1, with values ranging from 123 to 280 kg N ha-1 yr-1. Root and rhizome biomass varied among cultivars, with Ecoturf (26.9 Mg organic matter [OM] ha-1) and Latitude 34 (27.8 Mg OM ha-1) presenting greater root and rhizome mass than Florigraze (10.5 Mg OM ha-1) but similar to other varieties. Roots and rhizomes represented a significant portion of the total biomass and N pool, and further studies are needed to assess turnover of these tissues as well as their N contribution in grazing systems using grass-rhizoma peanut mixtures. [ABSTRACT FROM AUTHOR]

Introduction: Follow-up appointments (FUAs) are crucial to monitor proper healing in burn patients. This study aims to determine if there is a predictive relationship between the distance a patient lives from the burn center and FUA attendance This study also aims to determine if the COVID- 19 pandemic had any effect on FUA attendance at our burn center. Methods: Data was collected from the Burn Registry of a rural, ABA verified Regional Burn Center on patients who were admitted for an inpatient stay and discharged from January, 2019 through December, 2020. For each patient, we recorded demographic factors, home zip code, TBSA, length of inpatient stay, and FUA outcome. We split the resulting cohort in two separate ways by FUA status and pandemic status based on if they did/did not attend followup and were admitted before 3/9/20 (pre-pandemic) or after 3/9/20 (intra-pandemic). Chi Square Tests were used to determine if the rate of followup differed across pandemic status. We then ran Mann-Whitney U Tests to compare all the variables across both pandemic status and FUA status. Results: The cohort consisted of 511 patients. The pre-pandemic followup rate was 71.0% (n = 334) and the intra-pandemic followup rate was 71.2% (n = 177). This was not a statistically significant difference (X² = 0.003, df = 1, p = 0.96). The next analysis compared the variables across pandemic status. The only significantly different variable between the groups was an increased percentage of 2nd degree burns in the intra-pandemic group (U = 24656.0, p = 0.018). The next analysis compared the variables across FUA status. Age, distance to the burn clinic, and amount of 3rd degree burns significantly differed across FUA status groups (U = 22789.0, p = 0.007; U = 19946.5, p = 0.000; U = 22738.5, p = 0.037, respectively), indicating the group that did follow up was younger, lived closer, and had more 3rd degree burns. Conclusions: Furthermore, the results indicate that the distance a patient lives from our center is the highest predictive variable in determining whether or not a patient will attend followup. Applicability of Research to Practice: This research is especially applicable to practitioners in rural environments or those evaluating the reasons for lack of follow-up in burn care. [ABSTRACT FROM AUTHOR]

The article discusses the relationships between potential climate change impacts and disparities among Minnesota's environmental justice communities as of 2015, and it mentions the need for local governments in America to address climate-induced food insecurity issues. Land use strategies are mentioned, along with zoning for urban agriculture and access to land. Food production is examined, as well as the United Nations Declaration on the Rights of Indigenous Peoples.

Sustainable intensification of livestock systems implies greater efficiency in resource utilization resulting in greater output of products and other ecosystem services per unit of resource input. Integrating forage legumes into livestock systems is a viable way to reduce the input of industrial N fertilizer, reducing the use of fossil fuels and helping to mitigate global warming, a major problem during the Anthropocene. Some forage legumes have greater concentrations of secondary compounds, such as condensed tannins, that might reduce the emission of greenhouse gases (GHG) from ruminant eructation and excreta. Furthermore, forage legumes might enhance cattle performance because of greater nutritive value, resulting in greater production per unit of GHG released. Shortening the production cycle and improving cattle reproductive efficiency could have a major impact on reducing the overall carbon footprint of the system. Grazing systems with more diversified plant species are typically more resistant and resilient, adapting to current climate changes during the Anthropocene. Novel technologies might accelerate the development of future grazing systems using forage legumes as a key component. Breeding efforts for the next‐generation legumes must focus on adaptation and potential use for mitigation of negative environmental impacts. There are examples of successful integration of forage legumes into livestock systems in different regions of the world, with a major reduction in off‐farm inputs and maintaining the system productive. These successful examples could be used to increase adoption and improve the efficiency of current livestock systems. [ABSTRACT FROM AUTHOR]

Sepsis is a life-threatening immune response to infection in the body, eventually resulting in fatal organ failure. Current methods utilize blood cultures and quick-Sequential-Organ-Failure-Assessment (qSOFA), but there is a need for more accurate and time-sensitive diagnostic methods to improve survival rates. We present a 3D-printed microfluidic chip that bioconjugates antibodies CD69, CD64, and CD25 to channel surfaces to capture sepsis cells in blood samples and validate it with clinical samples (n = 125 septic, n = 10 healthy). Other variables were taken such as healthy volunteer blood samples and patient demographics to validate and confirm our device's diagnostic ability. Statistical differences were found between healthy volunteer and sepsis patient antigen cell counts (CD69 p-value < 0.001, CD64 p-value < 0.004, CD25 p-value < 0.0009), and were confirmed using principal component analysis. Demographics such as length of stay, age, culture results, and need for surgery also factored into sepsis detection on a smaller scale than the antigen cell counts. The receiver operating characteristic (ROC) analysis showed an area under the curve (AUC) of 0.989, 0.988, and 0.992 for CD69, CD64, and CD25, respectively, and a combined biomarker panel of 0.997. Overall, the device performed within a shorter time frame of 4 h compared to standard blood culture tests and was validated for use in detecting sepsis in patients. [Display omitted] [ABSTRACT FROM AUTHOR]