Search

Your search keyword '"Gaskell, S.J."' showing total 21 results
Start Over Author "Gaskell, S.J."
21 results on '"Gaskell, S.J."'

1. Phospholipids of Lactobacillus spp

Author

Drucker, D.B., Megson, G., Harty, D.W.S., Riba, I., and Gaskell, S.J.

Subjects
Lactobacillus -- Research, Phospholipids -- Research, Biological sciences
Abstract

The localization of individual molecular species of phospholipids in several Lactobacillus isolates were determined by mass spectrometry. Prominent anions indicated the existence of the phosphatidylglycerols PG(37:2), PG(36:2), PG(34:1), and PG(33:1). There were no phospholipids with nitrogen but diglycosyldiacylglycerol molecular species were present. An anion of m/z 759 was derived from a seemingly unique type of lipid.

Published
1995

2. Systematic integration of experimental data and models in systems biology.

Author

Li, P., Dada, J.O., Jameson, D., Spasic, I., Swainston, N., Carroll, K., Dunn, W., Khan, F., Messiha, E., Simeonides, E., Weichart, D., Winder, C., Broomhead, D.S., Goble, C.A., Gaskell, S.J., Kell, D.B., Westerhoff, H.V., Mendes, P., Paton, N.W., Li, P., Dada, J.O., Jameson, D., Spasic, I., Swainston, N., Carroll, K., Dunn, W., Khan, F., Messiha, E., Simeonides, E., Weichart, D., Winder, C., Broomhead, D.S., Goble, C.A., Gaskell, S.J., Kell, D.B., Westerhoff, H.V., Mendes, P., and Paton, N.W.

Abstract

Background: The behaviour of biological systems can be deduced from their mathematical models. However, multiple sources of data in diverse forms are required in the construction of a model in order to define its components and their biochemical reactions, and corresponding parameters. Automating the assembly and use of systems biology models is dependent upon data integration processes involving the interoperation of data and analytical resources.Results: Taverna workflows have been developed for the automated assembly of quantitative parameterised metabolic networks in the Systems Biology Markup Language (SBML). A SBML model is built in a systematic fashion by the workflows which starts with the construction of a qualitative network using data from a MIRIAM-compliant genome-scale model of yeast metabolism. This is followed by parameterisation of the SBML model with experimental data from two repositories, the SABIO-RK enzyme kinetics database and a database of quantitative experimental results. The models are then calibrated and simulated in workflows that call out to COPASIWS, the web service interface to the COPASI software application for analysing biochemical networks. These systems biology workflows were evaluated for their ability to construct a parameterised model of yeast glycolysis.Conclusions: Distributed information about metabolic reactions that have been described to MIRIAM standards enables the automated assembly of quantitative systems biology models of metabolic networks based on user-defined criteria. Such data integration processes can be implemented as Taverna workflows to provide a rapid overview of the components and their relationships within a biochemical system. © 2010 Li et al; licensee BioMed Central Ltd.

Published
2010
Full Text
View/download PDF

4. Protein turnover: the missing link in proteomics

Author

Pratt, J.M., primary, Gaskell, S.J., additional, Robertson, D.H.L., additional, Riba-Garcia, I., additional, Oliver, S.G., additional, Butler, P., additional, Petty, J., additional, Hayes, A., additional, and Beynon, R.J., additional

Published
2002
Full Text
View/download PDF

7. Proapoptotic Bid binds to monolysocardiolipin, a new molecular connection between mitochondrial membranes and cell death.

Author

Esposti, M. Degli, Cristea, I. M., Gaskell, S.J., Nakao, V., and Dive, C.

Subjects
CELL death, CARDIOLIPIN, MITOCHONDRIAL membranes, APOPTOSIS
Abstract

Recent evidence indicates that the mitochondrial lipid cardiolipin may be instrumental in the proapoptotic action of Bcl-2 family proteins on mitochondrial membranes, leading to the release of apoptogenic factors. However, contrasting evidence indicates that progressive loss of cardiolipin occurs during apoptosis. Here we show that Bid, a crucial proapoptotic protein that integrates the action of other Bcl-2 family members, exhibits discrete specificity for metabolites of cardiolipin, especially monolysocardiolipin (MCL). MCL, normally present in the remodelling of mitochondrial lipids, progressively increases in mitochondria during Fas-mediated apoptosis as a by-product of cardiolipin degradation, and also enhances Bid binding to membranes. MCL may thus play a crucial role in connecting lipid metabolism, relocation of Bid to mitochondria and integrated action of Bcl-2 proteins on mitochondrial membranes. We propose that Bid interaction with MCL ‘primes’ the mitochondrial outer membrane via segregation of lipid domains, facilitating membrane discontinuity and leakage of apoptogenic factors.Cell Death and Differentiation (2003) 10, 1300-1309, doi:10.1038/sj.cdd.4401306 Published online 1 August 2003 [ABSTRACT FROM AUTHOR]

Published
2003
Full Text
View/download PDF

10. Muscle growth in the chicken: a proteome-based approach.

Author

Beynon, R.J., Doherty, M., Hayter, J., El-Shafei, A., McLean, L., Robertson, D.H.L., and Gaskell, S.J.

Subjects
CHICKENS, MUSCLES, PROTEOMICS, SARCOPLASM
Abstract

Employs proteome analysis to examined the skeletal muscle in the domestic fowl, Gallus gallus. Identification of sarcoplasmic proteins in pectoral muscle; Analysis of intact sarcoplasmic proteins in pectoral muscle; Measurement of turnover rates of individual proteins.

Published
2003

19. Degraded and extended hopane derivatives (C27 to C35) as ubiquitous geochemical markers

Author

Dorsselaer, A.Van, primary, Ensminger, A., additional, Spyckerelle, C., additional, Dastillung, M., additional, Sieskind, O., additional, Arpino, P., additional, Albrecht, P., additional, Ourisson, G., additional, Brooks, P.W., additional, Gaskell, S.J., additional, Kimble, B.J., additional, Philp, R.P., additional, Maxwell, J.R., additional, and Eglinton, G., additional

Published
1974
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results