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2. Limited movement of Cucumber mosaic virus (CMV) in yellow passion flower in Brazil

Author

Gioria, R., Espinha, L. M., Rezende, JAM, Gaspar, J. O., Kitajima, E. W., Universidade de São Paulo (USP), and Universidade Estadual Paulista (Unesp)

Subjects
PTA-ELISA, PCR, P. edulis f. flavicarpa, Western blot, dot-blot hybridization
Abstract

Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2014-02-26T17:13:17Z No. of bitstreams: 1 WOS000175209200002.pdf: 505294 bytes, checksum: 5d5013a6fe49d8e6329ea86822fd5e6d (MD5) Made available in DSpace on 2014-02-26T17:13:17Z (GMT). No. of bitstreams: 1 WOS000175209200002.pdf: 505294 bytes, checksum: 5d5013a6fe49d8e6329ea86822fd5e6d (MD5) Previous issue date: 2002-04-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T14:03:19Z No. of bitstreams: 1 WOS000175209200002.pdf: 505294 bytes, checksum: 5d5013a6fe49d8e6329ea86822fd5e6d (MD5) Made available in DSpace on 2014-05-20T14:03:19Z (GMT). No. of bitstreams: 1 WOS000175209200002.pdf: 505294 bytes, checksum: 5d5013a6fe49d8e6329ea86822fd5e6d (MD5) Previous issue date: 2002-04-01 Symptoms of Cucumber mosaic virus (CMV) on yellow passion flower (Passiflora edulis f. flavicarpa) are characterized by bright yellow mottling on leaves, starting at random points on the vine and diminishing in intensity towards the tip, which becomes symptomless as it grows. To determine whether symptomless portions of vines are CMV-free or represent latent infection, leaves with and without symptoms were collected from infected vines in the field. Biological, serological (plate-trapped antigen enzyme-linked immunosorbent assay, PTA-ELISA), Western blot and dot-blot hybridization assays showed that portions of the vines without symptoms were CMV-free. Vegetatively propagated vines with symptoms showed remission of symptoms on newly developed leaves. One year later, no CMV was detected in the upper leaves of these plants. Mechanically inoculated passion flower seedlings behaved similarly; symptoms were shown by few leaves after inoculation. Afterwards, plants became symptomless and CMV was not detected in the upper leaves or root system, 40 or 85 days after inoculation. The mechanism responsible for remission of symptoms accompanied by CMV disappearance is not known. USP, ESALQ, Dept Entomol Fitopatol & Zool Agr, BR-13418900 Piracicaba, SP, Brazil UNESP, IBILCE, Dept Bot, BR-15054000 Sao Jose do Rio Preto, SP, Brazil UNESP, IBILCE, Dept Bot, BR-15054000 Sao Jose do Rio Preto, SP, Brazil

Published
2002

5. A degenerate primer allows amplification of part of the 3'-terminus of three distinct carlavirus species.

Author

Gaspar JO, Belintani P, Almeida AM, and Kitajima EW

Subjects
Conserved Sequence, Molecular Sequence Data, Sequence Analysis, DNA, Carlavirus genetics, DNA Primers genetics, Polymerase Chain Reaction methods, RNA, Viral genetics
Abstract

Sequences of the coat protein amino acids of definitive and tentative species of carlaviruses deposited in GenBank were aligned and a region of seven amino acids (GLGVPTE) was found to be conserved. The corresponding nucleotides were aligned, allowing the design of a degenerate primer that together with an oligo dT anti-sense primer, was effective for the detection of three distinct carlavirus species, two transmitted by aphids and one by whitefly. These primers have the advantage that about 940 nt from the 3'-terminus, comprising part of the CP gene (about 60%), the 11 K gene, and the terminal untranslated region can be amplified for sequencing. The fact that this amino acid sequence is conserved in almost all of the sequenced carlaviruses, allows the prediction that this primer pair will be useful as a diagnostic tool for carlavirus species.

Published
2008
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6. Subcellular localization of the coat protein in tobacco cells infected by cucumber mosaic virus isolated from Catharanthus roseus.

Author

Espinha LM and Gaspar JO

Subjects
Cucumovirus ultrastructure, Microscopy, Immunoelectron, Nicotiana metabolism, Nicotiana ultrastructure, Capsid metabolism, Cucumovirus metabolism, Plants, Toxic, Nicotiana virology
Abstract

Electron microscopy and immunolabelling with antiserum specific to cucumber mosaic virus coat protein were used to examine tobacco leaf cells infected by cucumber mosaic virus isolated from Catharanthus roseus (CMV-Cr). Crystalline and amorphous inclusions in the vacuoles were the most obvious cytological modifications seen. Immunogold labelling indicated that the crystalline inclusion was made up of virus particles and amorphous inclusions contained coat protein. Rows of CMV-Cr particles were found between membranes of dictyosomes, but membranous bodies and tonoplast-associated vesicles were not evident. Virus particles and/or free coat protein were easily detected in the cytoplasm by immunolabelling. No gold labelling was found within nuclei, chloroplasts and mitochondria.

Published
1999

7. Detection of snail hemocyanin in the whole hemolymph by serologically specific electron microscopy.

Author

Gaspar JO, Beriam LO, Airoldi LP, and Oliveira AR

Subjects
Animals, Hemocyanins immunology, Microscopy, Electron, Hemocyanins analysis, Hemolymph analysis, Snails analysis
Abstract

This study demonstrates the applicability of the serologically specific electron microscopy (SSEM) technique in the detection of hemocyanin molecules in the whole hemolymph of the snail, Megalobulimulus ovatus. The results are positive and easily reproducible. The SSEM might be useful as a technique for taxonomic studies of snails as well as to study structural aspects of their hemocyanin molecules.

Published
1983
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