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Objective: The objective of this study is to explore the patient characteristics and practice patterns of non-certified surgeons who treat Medicare patients in the United States., Background: Although most surgeons in the United States are board-certified, non-certified surgeons are permitted to practice in many locations. At the same time, surgical workforce shortages threaten access to surgical care for many patients. It is possible that noncertified surgeons may be able to help fill these access gaps. However, little is known about the practice patterns of non-certified surgeons., Methods: A 100% sample of Medicare claims data from 2014 to 2019 was used to identify practicing general surgeons. Surgeons were categorized as certified or non-certified in general surgery​​ based on data from the American Board of Surgery. Surgeon practice patterns and patient characteristics were analyzed., Results: A total of 2,097,206 patient cases were included in the study. These patients were treated by 16,076 surgeons, of which 6% were identified as non-certified surgeons. Compared with certified surgeons, non-certified surgeons were less frequently fellowship-trained (20.5% vs 24.2%, P =0.008) and more likely to be foreign medical graduates (14.5% vs 9.2%, P <0.001). Non-certified surgeons were more frequently practicing in for-profit hospitals (21.2% vs 14.2%, P <0.001) and critical access hospitals (2.2% vs 1.3%, P <0.001) and were less likely to practice in a teaching hospital (63.2% vs 72.4%, P <0.001). Compared with certified surgeons, non-certified surgeons treated more non-White patients (19.6% vs 14%, P <0.001) as well as a higher percentage of patients in the 2 lowest SES quintiles (36.2% vs 29.2%, P <0.001). Operations related to emergency admissions were more common amongst non-certified surgeons (68.8% vs 55.7%, P <0.001). There were no differences in sex or age of the patients treated by certified and non-certified surgeons., Conclusions: For Medicare patients, non-certified surgeons treated more patients who are non-White, of lower socioeconomic status, and in more rural, critical-access hospitals., Competing Interests: The authors report no conflicts of interest., (Copyright © 2024 Wolters Kluwer Health, Inc. All rights reserved.)

Introduction: Surgery residents who complete a nondesignated preliminary year have an additional year of training compared to those who begin as categorical residents. While this additional year is sometimes perceived negatively, these more experienced residents may outperform traditional categorical (TC) residents in their first year of training., Methods: Operative assessment ratings were recorded for first year categorical general surgery residents in the United States between 2015 and 2023 using the Society for Improving Medical and Professional Learning assessment platform. Residents were categorized based on the completion of a nondesignated preliminary year ("Previous Prelim" [PP]) or not ("Traditional Categorical"). Ratings were analyzed using generalized mixed effects models. Performance and autonomy outcomes were dichotomized: "less experience" or "more experience" and "no autonomy" or "some autonomy", respectively. Fixed effects included academic month and case complexity, while random effects included resident, faculty, program, and procedure., Results: A total of 34,353 evaluations from 86 general surgery programs were collected. Of these, 829 were evaluations from PP residents. Faculty ratings of PP versus TC revealed no differences in adjusted probabilities of achieving a "more experience" rating (82% versus 76%, P = 0.26) but a higher adjusted probability of achieving a "some autonomy" rating (88% versus 80%, P = 0.04) for PP compared to TC. Analysis of resident self-reported ratings revealed higher adjusted probabilities of a "more experience" rating (77% versus 50%, P = 0.01) and "some autonomy" rating (87% versus 73%; P = 0.02) for PP compared to TC., Conclusions: First year general surgery residents who previously completed a preliminary year have similar operative performance faculty ratings when compared to their peers., (Copyright © 2024 Elsevier Inc. All rights reserved.)

Background: Although most general surgery residency interviews remain virtual, the effect of this format remains understudied. Single-institution data have shown an increase in the number of applications received and interviews conducted with virtual interviewing but no change in the geographic backgrounds of interviewed or matched applicants. This study sought to compare national trends in geographic characteristics of general surgery applicants, interviewed applicants, and matched applicants between in-person and virtual application cycles., Study Design: A retrospective review of 7 general surgery residency programs from application years 2016-2019 (in-person) and 2020-2021 (virtual) was conducted. Data collected included birth year, sex, race, medical school state, and contact location at the time of application. Data were analyzed using generalized mixed effects linear models., Results: A total of 52,742 applicants, 4,550 interviewed applicants, and 329 matched applicants were included. During virtual application cycles, there were no increases in the average number of applicants (P = .25), interviewed applicants (P = .36), or matched (P = .84) applicants per year. Virtual cycles were associated with a larger proportion of interviews conducted with applicants from out-of-state medical schools (P < .01) and listing out-of-state contact locations (P < .01) compared with in-person application cycles. There were no significant geographic differences in matched applicants between virtual and in-person application cycles., Conclusion: Virtual application cycles had greater geographic diversity among interviewed applicants. However, similar differences were not seen in the geographic diversity of matched applicants. Additional efforts should focus on why no changes in the geographic diversity of matched applicants were identified., (Copyright © 2024 Elsevier Inc. All rights reserved.)

Objective: Workplace-based assessments (WBAs) play an important role in the assessment of surgical trainees. Because these assessment tools are utilized by a multitude of faculty, inter-rater reliability is important to consider when interpreting WBA data. Although there is evidence supporting the validity of many of these tools, inter-reliability evidence is lacking. This study aimed to evaluate the inter-rater reliability of multiple operative WBA tools utilized in general surgery residency., Design: General surgery residents and teaching faculty were recorded during 6 general surgery operations. Nine faculty raters each reviewed 6 videos and rated each resident on performance (using the Society for Improving Medical Professional Learning, or SIMPL, Performance Scale as well as the operative performance rating system (OPRS) Scale), entrustment (using the ten Cate Entrustment-Supervision Scale), and autonomy (using the Zwisch Scale). The ratings were reviewed for inter-rater reliability using percent agreement and intraclass correlations., Participants: Nine faculty members viewed the videos and assigned ratings for multiple WBAs., Results: Absolute intraclass correlation coefficients for each scale ranged from 0.33 to 0.47., Conclusions: All single-item WBA scales had low to moderate inter-rater reliability. While rater training may improve inter-rater reliability for single observations, many observations by many raters are needed to reliably assess trainee performance in the workplace., (Copyright © 2024 Association of Program Directors in Surgery. Published by Elsevier Inc. All rights reserved.)

Brazil stands as one of the world's leading producers of animal protein, ranking sixth in global egg production. However, estimated growth in production demand, along with environmental impacts, represents a potential threat to the sustainability of the food system. Methods for assessing and quantifying the environmental impacts generated by Brazilian egg production remain scarce, lacking current reports on comparative effects or guiding standards. The objective of this study was to conduct a life cycle assessment from cradle to farm gate, adhering to ISO 14040 and 14044 standards, for the battery cage egg production systems and associated products in Brazil, with the aim of supporting and promoting sustainability improvements in the Brazilian egg industry. The entire life cycle modeling and process sustainability analysis were executed using the openLCA software, integrated with the Ecoinvent database. Emissions related to egg production yielded results of 65.06 kg SO 2 eq., 27.74 kg N eq., 3086.71 kg CO 2 eq., 75,152.66 CTUe, 2.75 × 10 -5 CFC-11 eq., and 10,044.68 kg MJ eq. per ton of eggs produced. These findings can serve as comparative benchmarks for future studies and for analyzing data across different egg production systems in Brazil.

Competency-based medical education (CBME) is the future of medical education and relies heavily on high quality assessment. However, the current assessment practices employed by many general surgery graduate medical education training programs are subpar. Assessments often lack reliability and validity evidence, have low faculty engagement, and differ from program to program. Given the importance of assessment in CBME, it is critical that we build a better assessment system for measuring trainee competency. We propose that an ideal system of assessment is standardized, evidence-based, comprehensive, integrated, and continuously improving. In this article, we explore these characteristics and propose next steps to achieve such a system of assessment in general surgery., (Copyright © 2023 Association of Program Directors in Surgery. Published by Elsevier Inc. All rights reserved.)

Objective: Assessing surgical trainee operative performance is time- and resource-intensive. To maximize the utility of each assessment, it is important to understand which assessment activities provide the most information about a trainee's performance. The objective of this study is to identify the procedures that best differentiate performance for each general surgery postgraduate year (PGY)-level, leading to recommendations for targeted assessment., Design: The Society for Improving Medical Professional Learning (SIMPL) operative performance ratings were modeled using a multilevel Rasch model which identified the highest and lowest performing trainees for each PGY-level. For each procedure within each PGY-level, a procedural performance discrimination index was calculated by subtracting the proportion of "practice-ready" ratings of the lowest performing trainees from the proportion of "practice-ready" ratings of the highest performing trainees. Four-quadrant plots were created using the median procedure volume and median discrimination index for each PGY-level. All procedures within the upper right quadrant were considered "highly differentiating, high volume" procedures., Setting: This study was conducted across 70 general surgical residency programs who are members of the SIMPL collaborative., Participants: A total of 54,790 operative performance evaluations of categorical general surgery trainees were collected between 2015 and 2021. Trainees who had at least 1 procedure in common were included. Procedures with less than 25 evaluations per training year were excluded., Results: The total number of evaluations per procedure ranged from 25 to 2,131. Discrimination values were generated for 51 (PGY1), 54 (PGY2), 92 (PGY3), 105 (PGY4), and 103 (PGY5) procedures. Using the above criteria, a total of 12 (PGY1), 15 (PGY2), 22 (PGY3), 21 (PGY4), and 28 (PGY5) procedures were identified as highly differentiating, high volume procedures., Conclusions: Our study draws on national data to identify procedures which are most useful in differentiating trainee operative performance at each PGY-level. This list of procedures can be used to guide targeted assessment and improve assessment efficiency., (Copyright © 2023 Association of Program Directors in Surgery. Published by Elsevier Inc. All rights reserved.)

Objective: Feedback is critical for learning, however, gender differences exist in the quality of feedback that trainees receive. For example, narrative feedback on surgical trainees' end-of-block rotations differs based on trainee-faculty gender dyads, with female faculty giving higher quality feedback and male trainees receiving higher quality feedback. Though this represents evidence of gender bias in global evaluations, there is limited understanding of how much bias might be present in operative workplace-based assessments (WBAs). In this study, we explore the quality of narrative feedback among trainee-faculty gender dyads in an operative WBA., Design: A previously validated natural language processing model was used to examine instances of narrative feedback and assign a probability of being characterized as high quality feedback (defined as feedback which was relevant as well as corrective and/or specific). A linear mixed model was performed, with probability of high quality feedback as the outcome, and resident gender, faculty gender, PGY, case complexity, autonomy rating, and operative performance rating as explanatory variables., Participants: Analyses included 67,434 SIMPL operative performance evaluations (2,319 general surgery residents, 70 institutions) collected from September 2015 through September 2021., Results: Of 36.3% evaluations included narrative feedback. Male faculty were more likely to provide narrative feedback compared to female faculty. Mean probabilities of receiving high quality feedback ranged from 81.6 (female faculty-male resident) to 84.7 (male faculty-female resident). Model-based results demonstrated that female residents were more likely to receive high quality feedback (p < 0.01), however, there was no significant difference in probability of high quality narrative feedback based on faculty-resident gender dyad (p = 0.77)., Conclusions: Our study revealed resident gender differences in the probability of receiving high-quality narrative feedback following a general surgery operation. However, we found no significant differences based on faculty-resident gender dyad. Male faculty were more likely to provide narrative feedback compared to their female colleagues. Further research using general surgery resident-specific feedback quality models may be warranted., Competing Interests: DECLARATION OF COMPETING INTEREST None., (Copyright © 2023 Association of Program Directors in Surgery. Published by Elsevier Inc. All rights reserved.)

Introduction: Efforts to improve surgical resident well-being could be accelerated with an improved understanding of resident job demands and resources. In this study, we sought to obtain a clearer picture of surgery resident job demands by assessing how residents distribute their time both inside and outside of the hospital. Furthermore, we aimed to elucidate residents' perceptions about current duty hour regulations., Methods: A cross-sectional survey was sent to 1098 surgical residents at 27 US programs. Responses regarding work hours, demographics, well-being (utilizing the physician well-being index), and perceptions of duty hours in relation to education and rest, were collected. Data were evaluated using descriptive statistics and content analysis., Results: A total of 163 residents (14.8% response rate) were included in the study. Residents reported a median total patient care hours per week of 78.0 h. Trainees spent 12.5 h on other professional activities. Greater than 40% of residents were "at risk" for depression and suicide based on physician well-being index scores. Four major themes associated with education and rest were identified: 1) duty hour definitions and reporting mechanisms do not completely reflect the amount of work residents perform, 2) quality patient care and educational opportunities do not fit neatly within the duty hour framework, 3) resident perceptions of duty hours are impacted the educational environment, and 4) long work hours and lack of adequate rest negatively affect well-being., Conclusions: The breadth and depth of trainee job demands are not accurately captured by current duty hour reporting mechanisms, and residents do not believe that their current work hours allow for adequate rest or even completion of other clinical or academic tasks outside of the hospital. Many residents are unwell. Duty hour policies and resident well-being may be improved with a more holistic accounting of resident job demands and greater attention to the resources that residents have to offset those demands., (Copyright © 2023 Elsevier Inc. All rights reserved.)

We are the multi-institutional organization known as the Collaboration of Surgical Education Fellows (CoSEF). We've collectively reflected on our range of experiences across the country and identified 3 principles which promote a successful intern experience: (1) Own your patients; (2) Treat people like people; and (3) Take care of yourself., (Copyright © 2023 The Author(s). Published by Wolters Kluwer Health, Inc.)

Purpose: Trauma patients are at high risk of venous thromboembolism (VTE). We summarize the comparative efficacy and safety of anti-Xa-guided versus fixed dosing for low molecular weight heparin (LMWH) for the prevention of VTE in adult trauma patients., Methods: We searched Medline and Embase from inception through June 1, 2022. We included randomized controlled trials or observational studies comparing anti-Xa-guided versus fixed dosing of LMWH for thromboprophylaxis in adult trauma patients. We incorporated primary data from 2 large observational cohorts. We pooled effect estimates using a random-effects model. We assessed risk of bias using the ROBINS-I tool for observational studies and assessed certainty of findings using GRADE methodology., Results: We included 15 observational studies involving 10,348 patients. No randomized controlled trials were identified. determined that, compared to fixed LMWH dosing, anti-Xa-guided dosing may reduce deep vein thrombosis [adjusted odds ratio (aOR); 0.52, 95% CI: 0.40-0.69], pulmonary embolism (aOR: 0.48, 95% CI: 0.30-0.78) or any VTE (aOR: 0.54, 95% CI: 0.42-0.69), though all estimates are based on low certainty evidence. There was an uncertain effect on mortality (aOR: 1.06, 95% CI: 0.85-1.32) and bleeding events (aOR: 0.84, 95% CI: 0.50-1.39), limited by serious imprecision. We used several sensitivity and subgroup analyses to confirm the validity of our assumptions., Conclusion: Anti-Xa-guided dosing may be more effective than fixed dosing for prevention of deep vein thrombosis, pulmonary embolism, and VTE for adult trauma patients. These promising findings justify the need for a high-quality randomized study with the potential to deliver practice changing results., Competing Interests: M.C. is supported by a Tier 2 Research Chair in Venous Thrombosis and Cancer from the Department of Medicine at the University of Ottawa. M.C. reports research funding from Leo Pharma, Pfizer, and Bristol-Myers Squibb and honoraria from Pfizer, Bayer, Bristol-Myers Squibb, Sanofi, Leo Pharma, and Servier. B.R. is supported by a McMaster Department of Medicine Mid-Career Award. The remaining authors report no conflicts of interest., (Copyright © 2022 Wolters Kluwer Health, Inc. All rights reserved.)

Brazil is among the ten largest egg producers in the world. The domestic consumption of Brazilian eggs is 99.6%, the rest being exported to more than 82 countries, with an expectation of growth in the foreign market. The Brazilian egg industry has evolved considerably in recent decades, incorporating new technologies and smart practices. However, there is no assessment of how production could become more sustainable over the years. The LCA (Life Cycle Assessment) approach aims to recognize the polluting potential, identify the environmental impacts generated and reduce these impacts throughout production. On a global scale, researchers approach LCA as a constructive and quantitative technique, and there is great interest in implementing an LCA for the Brazilian egg production sector. With the aim of introducing the LCA methodology to the Brazilian egg industry, this review presents the concept and accounting structure of LCA through LCI (Life Cycle Inventory) and LCIA (Life Cycle Impact Assessment), based on the ISO 14040 and 14044:2006 standards, to quantify the environmental performance of production and identify areas for future improvement, thus promoting the environmental footprint of the Brazilian egg industry.

External egg quality is an essential parameter of egg production as it relates directly to economic losses. This study evaluated the spatial variability of external egg quality in five naturally ventilated caged vertical aviaries. Differences caused by bird age and thermal and luminous variability within aviaries during winter and summer were analyzed. Data on aviary air temperature, relative humidity, light intensity, and external egg quality were collected at evenly distributed points along the aviary length within three levels of cages. The experimental design was completely randomized in a factorial scheme. In the summer, the highest air temperature and lowest relative humidity were found in central cages, mainly in upper center cages; hens produced eggs with a lower weight and shape index in this area. Similar results were obtained in the winter. In the summer, eggs with lower shell weight and thickness were also produced by hens housed in the central cages, but in the winter, the opposite result was obtained. This study of the spatial variability of external egg quality proved efficient in detecting areas within an aviary with poor quality eggs; improvements to design and management in these areas could help management improve production efficiency and contribute to a sustainable egg supply., Competing Interests: The authors declare no conflicts of interest.

Gait scoring is a useful measure for evaluating broiler production efficiency, welfare status, bone quality, and physiology. The research objective was to track and characterize spatiotemporal and three-dimensional locomotive behaviors of individual broilers with known gait scores by jointly using deep-learning algorithms, depth sensing, and image processing. Ross 708 broilers were placed on a platform specifically designed for gait scoring and manually categorized into one of three numerical scores. Normal and depth cameras were installed on the ceiling to capture top-view videos and images. Four birds from each of the three gait-score categories were randomly selected out of 70 total birds scored for video analysis. Bird moving trajectories and 16 locomotive-behavior metrics were extracted and analyzed via the developed deep-learning models. The trained model gained 100% accuracy and 3.62 ± 2.71 mm root-mean-square error for tracking and estimating a key point on the broiler back, indicating precise recognition performance. Broilers with lower gait scores (less difficulty walking) exhibited more obvious lateral body oscillation patterns, moved significantly or numerically faster, and covered more distance in each movement event than those with higher gait scores. In conclusion, the proposed method had acceptable performance for tracking broilers and was found to be a useful tool for characterizing individual broiler gait scores by differentiating between selected spatiotemporal and three-dimensional locomotive behaviors.

Background: Trauma is a major risk factor for the development of a venous thromboembolism (VTE). After observing higher than expected VTE rates within our center's Trauma Quality Improvement Program data, we instituted a change in our VTE prophylaxis protocol, moving to enoxaparin dosing titrated by anti-Xa levels. We hypothesized that this intervention would lower our symptomatic VTE rates., Methods: Adult trauma patients at a single institution meeting National Trauma Data Standard criteria from April 2015 to September 2019 were examined with regards to VTE chemoprophylaxis regimen and VTE incidence. Two groups of patients were identified based on VTE protocol-those who received enoxaparin 30 mg twice daily without routine anti-Xa levels ("pre") versus those who received enoxaparin 40 mg twice daily with dose titrated by serial anti-Xa levels ("post"). Univariate and multivariate analyses were performed to define statistically significant differences in VTE incidence between the two cohorts., Results: There were 1698 patients within the "pre" group and 1406 patients within the "post" group. The two groups were essentially the same in terms of demographics and risk factors for bleeding or thrombosis. There was a statistically significant reduction in VTE rate (p = 0.01) and deep vein thrombosis rate (p = 0.01) but no significant reduction in pulmonary embolism rate (p = 0.21) after implementation of the anti-Xa titration protocol. Risk-adjusted Trauma Quality Improvement Program data showed an improvement in rate of symptomatic pulmonary embolism from fifth decile to first decile., Conclusion: A protocol titrating prophylactic enoxaparin dose based on anti-Xa levels reduced VTE rates. Implementation of this type of protocol requires diligence from the physician and pharmacist team. Further research will investigate the impact of protocol compliance and time to appropriate anti-Xa level on incidence of VTE., Level of Evidence: Therapeutic/care management, Level IV., (Copyright © 2021 Wolters Kluwer Health, Inc. All rights reserved.)

Heat stress is one of the most detrimental environmental challenges affecting the biological process and the related production performance of farm animals, especially in poultry. Commercial laying hens have been bred (selected) for high egg production, resulting in increased sensitivity to heat stress due to breeding-linked metabolic heat production. In addition, laying hens are prone to heat stress due to their inadequate species-specific cooling mechanisms resulting in low heat tolerance. In addition, hens have no sweat glands and feathering covers almost their entire body to minimize body heat loss. The poultry industry and scientists are developing cooling methods to prevent or reduce heat stress-caused damage to chicken health, welfare, and economic losses. We have designed and tested a cooling system using perches, in which chilled water (10 °C) circulates through a conventional perch passing through the layer cages to offer the cooling potential to improve hen health, welfare, and performance during acute and chronic periods of heat stress (35 °C). This review summarizes the outcomes of a multi-year study using the designed cooled perch system. The results indicate that conducting heat from perching hens directly onto the cooled perch system efficiently reduces heat stress and related damage in laying hens. It provides a novel strategy: perches, one key furnishment in cage-free and enriched colony facilities, could be modified as cooling devices to improve thermal comfort for hens during hot seasons, especially in the tropical and subtropical regions.

Foam accumulation in swine manure deep-pits has been linked to explosions and flash fires that pose devastating threats to humans and livestock. It is clear that methane accumulation within these pits is the fuel for the fire; it is not understood what microbial drivers cause the accumulation and stabilization of methane. Here, we conducted a 13-month field study to survey the physical, chemical, and biological changes of pit-manure across 46 farms in Iowa. Our results showed that an increased methane production rate was associated with less digestible feed ingredients, suggesting that diet influences the storage pit's microbiome. Targeted sequencing of the bacterial 16S rRNA and archaeal mcrA genes was used to identify microbial communities' role and influence. We found that microbial communities in foaming and non-foaming manure were significantly different, and that the bacterial communities of foaming manure were more stable than those of non-foaming manure. Foaming manure methanogen communities were enriched with uncharacterized methanogens whose presence strongly correlated with high methane production rates. We also observed strong correlations between feed ration, manure characteristics, and the relative abundance of specific taxa, suggesting that manure foaming is linked to microbial community assemblage driven by efficient free long-chain fatty acid degradation by hydrogenotrophic methanogenesis., Competing Interests: The authors have declared that no competing interests exist.

Determining ingestive behaviors of dairy cows is critical to evaluate their productivity and health status. The objectives of this research were to (1) develop the relationship between forage species/heights and sound characteristics of three different ingestive behaviors (bites, chews, and chew-bites); (2) comparatively evaluate three deep learning models and optimization strategies for classifying the three behaviors; and (3) examine the ability of deep learning modeling for classifying the three ingestive behaviors under various forage characteristics. The results show that the amplitude and duration of the bite, chew, and chew-bite sounds were mostly larger for tall forages (tall fescue and alfalfa) compared to their counterparts. The long short-term memory network using a filtered dataset with balanced duration and imbalanced audio files offered better performance than its counterparts. The best classification performance was over 0.93, and the best and poorest performance difference was 0.4-0.5 under different forage species and heights. In conclusion, the deep learning technique could classify the dairy cow ingestive behaviors but was unable to differentiate between them under some forage characteristics using acoustic signals. Thus, while the developed tool is useful to support precision dairy cow management, it requires further improvement.

Composting has been used to dispose of animal mortalities and infected materials, such as manure and feed, during major animal disease outbreaks. In this study, we adapted the plastic-wrapped mortality composting system developed by the Canadian Food Inspection Agency during the 2004 highly pathogenic avian influenza outbreak to compost swine mortalities. The goals of the study were to evaluate the performance of the plastic-wrapped composting system to dispose of swine mortalities and to field test its ability to eliminate the spread of airborne pathogens through the aeration ducts. Two cover materials, ground cornstalks and woodchips, were tested using passively and actively aerated composting sheds. The mortalities were inoculated with Salmonella spp. and vaccine strains of Bovine herpesvirus-1 and Bovine viral diarrhea virus. Air samples collected from the upper aeration duct (air outlet) during the first 10 d of composting were negative for Salmonella and the viruses tested, which indicated that aerosol transmission of the pathogens was limited. The aeration plenum placed under the mortalities helped to keep conditions aerobic, as O 2 concentrations of both passively and actively aerated test units were above 11%. Actively aerated cornstalks had the highest degree-hours (1,462 °C h d -1 ), which was followed by passively aerated cornstalks (1,312 °C h d -1 ), actively aerated woodchips (1,303 °C h d -1 ), and passively aerated woodchips (1,062 °C h d -1 ). After a 7-wk composting period, all three pathogens were inactivated based on quantitative polymerase chain reaction test results. The mortalities were not inoculated with the African swine fever virus, but temperature data showed that if they were, the system had the potential to eliminate this virus., (© 2021 The Authors. Journal of Environmental Quality © 2021 American Society of Agronomy, Crop Science Society of America, and Soil Science Society of America.)

Neonatal piglets can experience both a decrease in body temperature and hypoxia, increasing risks for pre-weaning mortality. This research evaluated the effects of drying and providing supplemental oxygen to newborn piglets on rectal temperature (RT) over the first 24 h after birth. The study used a CRD with three Intervention Treatments (IT; applied at birth): Control (no intervention), Drying (dried using a desiccant), Oxygen [dried using a desiccant and placed in a chamber (at 40% oxygen concentration) for 20 min]. A total of 42 litters (485 piglets) were randomly allotted to treatments at the start of farrowing. At birth, each piglet was given a numbered ear tag, weighed, and the treatment was applied; RT was measured at 0, 20, 30, 45, 60, 120, and 1440 min after birth. Blood was collected from one piglet from each birth weight quartile within each litter at 24 h after birth to measure plasma immunocrit concentration. There was no effect ( P  > 0.05) of IT on piglet RT at 0 or 1440 min after birth. Between 20 and 60 min after birth, piglet RT was lower ( P  ≤ 0.05) for the Control than the Drying treatment, with the Oxygen treatment being intermediate and different ( P  ≤ 0.05) from the other two IT. The effect of piglet birth weight on responses to IT were evaluated by classifying piglets into Birth Weight Categories (BWC): Light (<1.0 kg), Medium (1.0 to 1.5 kg), or Heavy (>1.5 kg). There were IT by BWC interactions ( P  ≤ 0.05) for piglet RT at all measurement times between 20 and 120 min after birth. Relative to the Control, the effects of the Drying and Oxygen treatments on RT were greater ( P  ≤ 0.05) for Light than heavier piglets. Plasma immunocrit concentrations tended ( P  = 0.07) to be greater for piglets on the Control treatment compared to the other two IT and were lower ( P  ≤ 0.05) for Light than Heavy piglets, with Medium piglets being intermediate and different ( P  ≤ 0.05) to the other BWC. In conclusion, drying piglets at birth reduced the extent and duration of RT decline in piglets in the early postnatal period compared to undried piglets, especially for those of low birth weight. However, the combination of drying and placing piglets in an oxygen-rich environment provided no additional benefit over drying alone., (© The Author(s) 2021. Published by Oxford University Press on behalf of the American Society of Animal Science.)

Consciousness is central to animal welfare concerns. Its assessment is most often conducted based on behavior, with a poor understanding of the correspondence between behavior and the neurobiological processes that underlie the subjective experience of consciousness. Recording of brain electrical activity using electrodes placed under the skull improves EEG recording by minimizing artifacts from muscular or cardiac activities, and it can now be combined with wireless recording in free-moving animals. This experiment investigated the correspondence between wireless 'under the skull' epidural EEG and the behavior of 18 five-week-old female piglets undergoing nitrous oxide (N 2 O) or carbon dioxide (CO 2 ) gradual fill gas euthanasia at 25% replacement rate per minute of the chamber volume. Piglets exposed to CO 2 had a peak in EEG total power ('Ptot') during the flailing stage, whereas piglets exposed to N 2 O had a higher EEG 95% spectral edge frequency ('F95') during their initial explorative behavior phase and a drop in EEG median frequency ('F50') after loss of posture. Loss of posture without righting attempt, as the last behavioral state observed during euthanasia, preceded the onset of transitional EEG on average by 0.9 and 3.1 min (for CO 2 and N 2 O treatments, respectively), and the onset of isoelectric EEG by 4.5 and 6.2 min (for CO 2 and N 2 O treatments, respectively). Paddling movements occurred shortly before and during transitional EEG but never during isoelectric EEG, whereas gasps persisted after the EEG had become isoelectric. The dynamics of EEG spectral changes were complex to interpret in relation to the degree of consciousness, but isoelectric EEG as an unequivocal indicator of unconsciousness appeared several minutes after loss of posture with no righting attempt. This leaves a window of uncertainty in regards to the potential for consciousness after loss of posture during gradual fill gas euthanasia in piglets., (Copyright © 2020 The Authors. Published by Elsevier Inc. All rights reserved.)

Understanding the air temperature distribution, ammonia (NH 3 ) and carbon dioxide (CO 2 ) levels in poultry housing systems are crucial to poultry health, welfare, and productivity. In this study, 4 Intelligent Portable Monitoring Units and 7 temperature sensors were installed inside and between the cages and above 2 minimum ventilation fans of a commercial stacked-deck cage laying hen house in the Midwest United States (425,000 laying hens) to continuously monitor the interior environment over a 6-month period. During cold conditions (March 12th-May 22nd), there was a variation noted, with barn center temperatures consistently being highest in the longitudinal and lateral direction (P < 0.001) and the top floor deck warmer than the bottom floor (P < 0.05). During hotter conditions (May 23rd-July 26th), the interior thermal environment was more uniform than during the winter, resulting in a difference only in the longitudinal direction. The daily CO 2 and NH 3 concentrations were 400 to 4,981 ppm and 0 to 42.3 ppm among the 4 sampling locations, respectively. Both CO 2 and NH 3 decreased linearly with increasing outside temperatures. The mean NH 3 and CO 2 concentrations varied with sampling locations and with the outside temperatures (P < 0.001). For CO 2 , the minimum ventilation sidewall had lower values than those measured in the barn's center (P < 0.05) during cold weather, while the barn center and the manure room sidewall consistently measured the highest concentrations during warmer weather (P < 0.05). For NH 3 , the tunnel ventilation inlet end consistently had the lowest daily concentrations, whereas the in-cage and manure drying tunnel sidewall locations measured the highest concentrations (P < 0.001). Higher NH 3 and CO 2 concentrations were recorded within the cage than in the cage aisle (P < 0.05). The highest NH 3 concentration of 42 ppm was recorded above the minimum exhaust fan adjacent to the manure drying tunnel, which indicated that higher pressure (back pressure) in the manure drying tunnel allowed air leakage back into the production area through nonoperating sidewall fan shutters., (Copyright © 2020. Published by Elsevier Inc.)

One measure of the thermal status of poultry is cloacal temperature measured with a cloacal thermometer; however, this method requires handling the bird, is invasive, and can be stressful. Infrared thermography is an alternative means for assessing bird thermal status. The objective of this study was to investigate the body temperature response of pullets subjected to different environmental air temperatures during the growing phase and to evaluate the relationship between the cloacal temperature and the body parts surface temperature. A total of 648 chicks (Lohmann LSL Lite) were used in 2 different phases, phase I (day 1 through 6 wk of age) and phase II (week 7 through 17). During phase I, chicks were reared at 1 of 3 different thermal environments: thermal comfort (35°C-19°C), mild heat stress (38°C-22°C), or mild cold stress (28°C-17°C). In phase II, pullets were randomly redistributed to 1 of 4 daytime temperature treatments: 20°C; 25°C; 30°C; and 35°C, all with night time temperature of 20°C. Cloacal temperature and body surface temperature for 8 parts (head, eye, comb, chest, back, wing, leg, head area, and body area) were obtained weekly from 4 to 2 birds per treatment, respectively, during phase II. There were no effects for the interactions between the 2 experimental phases for cloacal and body parts surface temperature. There was a strong correlation (P < 0.001) between cloacal temperature and each body part temperature; cloacal temperature followed a quadratic response to environmental air temperature treatments. Pullets subjected to 35°C/20°C and 30°C/20°C had the highest body parts temperatures compared with the other 2 treatments (P < 0.05). The leg surface temperature was greatest in all treatments, and the chest the lowest. Regression between cloacal and body parts temperature had a 95% predictive accuracy of better than 0.4°C, suggesting a useful alternative to direct cloacal temperature measurement., (Copyright © 2020 The Authors. Published by Elsevier Inc. All rights reserved.)

Livestock production is associated with several gaseous pollutant emissions to the environment. These emissions can degrade local and regional air quality, contribute to surface water eutrophication and acid rain, and contribute to the greenhouse gas footprint of the production sector. Modern production systems must balance animal welfare and environmental pollution potential with economic reality, which is a great challenge to maintain as global demand for animal protein increases. Accordingly, gaseous emission technologies were the main target for this research, in which mitigating gas emissions of ammonia, nitrous oxide, and methane from pig production facilities via slurry aeration system was tested. Five treatments with different airflow rates in the test room were examined continuously over a period of 6 weeks and the results were compared with the control room. Test results indicate that the highest mitigation potentials were 12, 57.6, and 10.4% for nitrous oxide, methane, and ammonia, respectively. Subsequently directing exhaust air into a sulfuric acid air scrubber at 3.0 pH further reduced total ammonia emissions by 80 to 87%.

This study examined the effect of water-chilled perches on hen production and physiological responses to induced molt during elevated temperatures. A total of 288White Leghorns at 82 wk of age were housed in 36 cages of 6 banks. Each bank was assigned to 1 of 3 treatments: cooled perches, air perches, and no perches. The hens were subjected to 2 heat episodes during their first laying cycle at week 21 to 35 and week 73 to 80, respectively. The hens were subjected to a 28 D nonfasted molting regimen starting at 85 wk of age. Cyclic heat of 32°C (6:00 am to 6:00 pm) was applied daily during the molting period. After molt, hens were returned to a layer diet and housed under thermoneutral condition. Two birds per cage were monitored for BW change during molt. Egg production was recorded daily. Feed utilization was measured during molt at 86 and 88 wk of age. Egg weight and eggshell traits were examined at 84 wk (pre-molt) and post-molt at 92, 96, and 104 wk of age. Rectal temperature and blood samples were collected from 2 birds per cage at the end of molt. Blood samples were used for determining heterophil/lymphocyte ratio, corticosterone, and thyroid hormones. Plumage condition was examined at 22 wk post-molt. Compared to control and air perch hens, cooled perch hens had higher feed usage and greater BW loss, lower heterophil/lymphocyte ratios (P < 0.05) with no difference in thyroid hormones and corticosterone at the end of molt. Cooled perch hens also had higher egg production beginning at 98 wk of age (Ptreatment*age < 0.0001) than control hens and sometimes the air-perch hens. Cooled perch hens had higher rectal temperature than control but not air perch hens at end of molt. Moreover, cooled perch hens had better breast feather scores than air perch hens but worse vent plumage (P ≤ 0.05) than both control and air perch hens. These results indicate that the provision of cooled perches assists hens with better adaptation to stressors, such as induced molt plus heat exposure, resulting in improved post-molt egg production., (Published by Oxford University Press on behalf of Poultry Science Association 2019.)

We examined the effects of water-chilled perches as cooling devices on hen performance during 2 summers using daily cyclic heat. White Leghorns, 17 wk of age, were assigned to 36 cages arranged into 6 banks. The banks were assigned to cooled perches, air perches, and no perches resulting in 2 replicate banks and 12 cages per treatment. Chilled water (10°C) was circulated through the cooled perches during heat episodes. Daily cyclic heat of 35°C was applied from 0600 to1800 h with a lowering of temperature to 28°C from 1800 to 0600 h during the 2014 and 2015 summers when hens were 21 to 35 and 73 to 80 wk of age, respectively. Mortality and egg production were recorded daily. Feed utilization, egg weight, and shell quality traits were measured at 4-wk intervals during the heat episodes and at 8-wk intervals during thermoneutrality. Body weight was determined at 17, 35, 72, and 80 wk of age and physical condition at 80 wk of age. At several ages during the heat episodes, cooled perch hens had increased egg production (P < 0.0001) and feed usage (P < 0.04) as compared to both air perch and control hens. The cooled perch hens had higher BW at 35 and 72 wk of age (Ptreatment*age = 0.03) and lower cumulative mortality (P = 0.02) than control hens but not air perch hens. Eggs from cooled perch hens had overall heavier weights (P < 0.0001) and higher breaking force (P < 0.0001) than eggs from the other two group hens. Greater eggshell percentage (Ptreatment*age = 0.03) and eggshell thickness (Ptreatment*age = 0.01) occurred at some ages during the 2 heat episodes as compared to the other 2 treatments. Nail length, feet hyperkeratosis, and overall feather score were similar among treatments. These results indicate that cooled perch ameliorates the negative effects of heat stress on egg traits and performance without influencing the physical condition of hens., (Published by Oxford University Press on behalf of Poultry Science Association 2019.)

The objective of this study was to evaluate the effects of different environment conditions on productive performance and surface temperatures of Japanese quail (Coturnix coturnix japonica) during the initial stage of laying. In environmental controlled chambers, the birds were subjected to different temperatures and air velocities at the feeder. A total of 216 Japanese quails were distributed randomly in 2 galvanized wire cages, with 3 partitions each and 27 birds/cage. The experimental design consisted of randomized blocks with 2 treatments (air velocity at the feeder: 0, 1, 2, and 3 m/s and air temperature: 17, 23, 29, and 35°C) and 6 replicates. The productive performance was analyzed statistically (Sigma Plot 12.0) by 2-way ANOVA, with treatment means separated by the Tukey test (P < 0.05). To evaluate the main effects and interactions of the factors, the Holm-Sidak multiple comparisons test was performed using a mild condition as the control group (0 m/s). Feed intake did not differ (P > 0.05) among birds reared at temperatures of 23, 29, and 35°C, but higher feed intake was noted at 17°C. The mean values of egg production increased significantly (P < 0.05) with increased air velocity levels. It was observed that there was an increase in egg production and feed intake with the intensification of air velocity at the feeder, regardless of ambient temperature. Egg weight and feed conversion were not affected by air velocity treatments (P > 0.05). There was a significant positive correlation between air temperature and mean surface temperature and head surface temperature. In contrast, a significant negative correlation was observed between air velocity and mean surface temperature and head surface temperature. Productive performance was affected by temperature and air velocity, except for egg weight and feed conversion, which was not influenced by air velocity. Air velocity is important in removing heat from the surface of birds., (© 2019 Poultry Science Association Inc.)

We assessed the effects of water-chilled perches as a cooling device on the physiological parameters of caged laying hens exposed to 2 cyclic heating episodes. White Leghorns, 17 wk of age, were randomly assigned to 36 cages of 6 banks placed in the same room. Each bank was randomly assigned to 1 of 3 treatments: cooled perch (CP), air perch (AP), and no perch (CTRL) resulting in 2 replicate banks and 12 cages per treatment. Chilled water (10°C) circulated through the CP during heat periods when hens were 21 wk to 35 wk and 73 wk to 80 wk of age, respectively. During the heating episodes, hens were submitted to a daily cyclic temperature regimen of 35°C (0600 h to 1800 h) and 28°C (1800 h to 0600 h). Rectal temperature, packed cell volume, heterophil to lymphocyte (H/L) ratio, and plasma levels of triiodothyronine (T3), thyroxine (T4), interleukin (IL)-6, IL-10, immunoglobulin (Ig) Y, interferon (IFN)-γ, and heat shock protein (HSP) 70 were measured on the last day of the 2 heating episodes. At the end of the first heating episode, CP hens had lower rectal temperature (P = 0.02) than both AP and CTRL hens. The CP hens also had lower HSP 70 (P = 0.04) than CTRL hens but not AP hens. At the end of the second heating episode, the CP hens had lower rectal temperature (P = 0.02) and circulating H/L ratio (P = 0.01) than both AP and CTRL hens. The CP hens also had higher levels of T3 (P = 0.002) and T3/T4 ratio (P = 0.0006) than CTRL hens but not AP hens, with a greater packed cell volume than AP hens (P = 0.02) but not CTRL hens. Cytokines and IgY levels were similar among treatments. These results indicate that CP hens were better able to cope with cyclic heat stress than CTRL and sometimes AP hens as noted by the beneficial effects on rectal temperature, thyroid activity, HSP, and H/L ratio., (Published by Oxford University Press on behalf of Poultry Science Association 2019.)

Extreme weather conditions challenge pig thermoregulation during transport and are addressed by the National Pork Board (NPB) Transport Quality Assurance ® (TQA) program that provides guidelines for trailer boarding, bedding, and misting. These guidelines are widely applied, yet very little is known about the microenvironment within the trailer. In this study, TQA guidelines (V4) were evaluated via extensive thermal environment measurements during transport in order to evaluate spatial variability and implications on ventilation pattern. Effects of trailer management strategies including bedding, boarding, and misting were examined and the trailer was monitored for interior temperature rise and THI responses within six separate zones. The trailer thermal environment was not uniformly distributed in the colder trips with the top front and bottom zones were the warmest, indicating these zones had the majority of outlet openings and experienced air with accumulated sensible and latent heat of the pigs. Relatively enhanced thermal environment uniformity was observed during hot trips, suggesting that ventilation patterns and ventilation rate were different for colder vs. warmer weather conditions. Misting applied prior to transport cooled interior air temperature, but also created high THI conditions in some cases. Neither boarding and bedding combinations in the TQA nor boarding position showed impacts on trailer interior temperature rise or spatial distribution of temperature inside the trailer.

Appropriate ventilation of poultry facilities is critical for achieving optimum performance. Ventilation promotes good air exchange to remove harmful gases, excessive heat, moisture, and particulate matter. In a turkey brooder barn, carbon dioxide (CO2) may be present at higher levels during the winter due to reduced ventilation rates to maintain high temperatures. This higher CO2 may negatively affect turkey poult performance. Therefore, the objective of this study was to evaluate the effects of subjecting tom turkey poults (commercial Large White Hybrid Converters) to different constant levels of atmospheric CO2 on their growth performance and behavior. In three consecutive replicate trials, a total of 552 poults were weighed post-hatch and randomly placed in 3 environmental control chambers, with 60 (Trial 1) and 62 (Trials 2 and 3) poults housed per chamber. They were reared with standard temperature and humidity levels for 3 wks. The poults were exposed to 3 different fixed CO2 concentrations of 2,000, 4,000, and 6,000 ppm throughout each trial. Following each trial (replicate), the CO2 treatments were switched and assigned to a different chamber in order to expose each treatment to each chamber. At the end of each trial, all poults were sent to a local turkey producer to finish growout. For each trial, individual body weight and group feed intake were measured, and mortality and behavioral movement were recorded. Wk 3 and cumulative body weight gain of poults housed at 2,000 ppm CO2 was greater (P < 0.05) than those exposed to 4,000 and 6,000 ppm CO2. Feed intake and feed conversion were unaffected by the different CO2 concentrations. No significant difference in poult mortality was found between treatments. In addition, no effect of CO2 treatments was evident in the incidence of spontaneous turkey cardiomyopathy for turkeys processed at 19 wk of age. Poults housed at the lower CO2 level (2,000 ppm) demonstrated reduced movement compared with those exposed to the 2 higher CO2 concentrations.

Current methods of euthanizing piglets are raising animal welfare concerns. Our experiment used a novel two-step euthanasia method, using nitrous oxide (N₂O) for six minutes and then carbon dioxide (CO₂) on compromised 0- to 7-day-old piglets. A commercial euthanasia chamber was modified to deliver two euthanasia treatments: the two-step method using N₂O then CO₂ (N₂O treatment) or only CO₂ (CO₂ treatment). In Experiment 1, 18 piglets were individually euthanized. In Experiment 2, 18 groups of four to six piglets were euthanized. In the N₂O treatment, piglets lost posture, indicating the onset of losing consciousness, before going into CO₂ where they showed heavy breathing and open-mouth breathing; whereas piglets in the CO₂ treatment did not lose posture until after exhibiting these behaviors ( p &le; 0.004). However, piglets in the N₂O treatment took longer to lose posture compared to the CO₂ treatment ( p < 0.001). Piglets in the N₂O treatment displayed more behavioral signs of stress and aversion: squeals/minute ( p = 0.004), escape attempts per pig ( p = 0.021), and righting responses per pig ( p = 0.084) in a group setting. In these regards, it cannot be concluded that euthanizing piglets for 6 min with N₂O and then CO₂ is more humane than euthanizing with CO₂ alone.

The tilt angle (angle of repose) of an AFM cantilever relative to the surface it is interrogating affects the effective stiffness of the cantilever as it analyzes the surface. For typical AFMs and cantilevers that incline from 10° to 15° tilt, this is thought to be a 3%-7% stiffness increase correction. While the theoretical geometric analysis of this effect may have reached a consensus that it varies with cos -2 θ, there is very little experimental evidence to confirm this using AFM cantilevers. Recently, the laser Doppler vibrometry thermal calibration method utilized at NIST has demonstrated sufficient stiffness calibration accuracy, and precision to allow a definitive experimental confirmation of the particular trigonometric form of this tilt effect using a commercial microfabricated AFM cantilever specially modified to allow strongly tilted (up to 15°) effective cantilever stiffness measurements.

The exploitation of nanoscale size effects to create new nanostructured materials necessitates the development of an understanding of relationships between molecular structure, physical properties and material processing at the nanoscale. Numerous metrologies capable of thermal, mechanical, and electrical characterization at the nanoscale have been demonstrated over the past two decades. However, the ability to perform nanoscale molecular/chemical structure characterization has only been recently demonstrated with the advent of atomic-force-microscopy-based infrared spectroscopy (AFM-IR) and related techniques. Therefore, we have combined measurements of chemical structures with AFM-IR and of mechanical properties with contact resonance AFM (CR-AFM) to investigate the fabrication of 20-500 nm wide fin structures in a nanoporous organosilicate material. We show that by combining these two techniques, one can clearly observe variations of chemical structure and mechanical properties that correlate with the fabrication process and the feature size of the organosilicate fins. Specifically, we have observed an inverse correlation between the concentration of terminal organic groups and the stiffness of nanopatterned organosilicate fins. The selective removal of the organic component during etching results in a stiffness increase and reinsertion via chemical silylation results in a stiffness decrease. Examination of this effect as a function of fin width indicates that the loss of terminal organic groups and stiffness increase occur primarily at the exposed surfaces of the fins over a length scale of 10-20 nm. While the observed structure-property relationships are specific to organosilicates, we believe the combined demonstration of AFM-IR with CR-AFM should pave the way for a similar nanoscale characterization of other materials where the understanding of such relationships is essential.

We assessed the effects of chilled water cooling perches on hen performance and physiological and behavioral parameters under "natural" high temperatures during the 2013 summer with a 4-hour acute heating episode. White Leghorns at 16 wk of age (N = 162) were randomly assigned to 18 cages (n = 9) arranged into 3 units. Each unit was assigned to one of the 3 treatments through 32 wk of age: 1) cooled perches, 2) air perches, and 3) no perches. Chilled water (10°C) was circulated through the cooled perches when cage ambient temperature exceeded 25°C. At the age of 27.6 wk, hens were subjected to a 4-hour acute heating episode of 33.3°C and plasma corticosterone was determined within 2 hours. Egg production was recorded daily. Feed intake and egg and shell quality were measured at 5-week intervals. Feather condition, foot health, adrenal and liver weights, plasma corticosterone, and heat shock protein 70 mRNA were determined at the end of the study at 32 wk of age. The proportion of hens per cage perching, feeding, drinking, panting, and wing spreading was evaluated over one d every 5 wks and on the d of acute heat stress. There were no treatment effects on the measured physiological and production traits except for nail length. Nails were shorter for cooled perch hens than control (P = 0.002) but not air perch hens. Panting and wing spread were observed only on the day of acute heat stress. The onset of both behaviors was delayed for cooled perch hens, and they perched more than air perch hens following acute heat stress (P = 0.001) and at the age 21.4 wk (P = 0.023). Cooled perch hens drank less than control (P = 0.019) but not air perch hens at the age 21.4 wk. These results indicate that thermally cooled perches reduced thermoregulatory behaviors during acute heat stress, but did not affect their performance and physiological parameters under the ambient temperature imposed during this study., (Published by Oxford University Press on behalf of Poultry Science Association 2016. This work is written by (a) US Government employee(s) and is in the public domain in the US.)

Laboratory notebooks have been a staple of scientific research for centuries for organizing and documenting ideas and experiments. Modern laboratories are increasingly reliant on electronic data collection and analysis, so it seems inevitable that the digital revolution should come to the ordinary laboratory notebook. The most important aspect of this transition is to make the shift as comfortable and intuitive as possible, so that the creative process that is the hallmark of scientific investigation and engineering achievement is maintained, and ideally enhanced. The smart electronic laboratory notebooks described in this paper represent a paradigm shift from the old pen and paper style notebooks and provide a host of powerful operational and documentation capabilities in an intuitive format that is available anywhere at any time.

Calibration of the flexural spring constant for atomic force microscope (AFM) colloid probe cantilevers provides significant challenges. The presence of a large attached spherical added mass complicates many of the more common calibration techniques such as reference cantilever, Sader, and added mass. Even the most promising option, AFM thermal calibration, can encounter difficulties during the optical lever sensitivity measurement due to strong adhesion and friction between the sphere and a surface. This may cause buckling of the end of the cantilever and hysteresis in the approach-retract curves resulting in increased uncertainty in the calibration. Most recently, a laser Doppler vibrometry thermal method has been used to accurately calibrate the normal spring constant of a wide variety of tipped and tipless commercial cantilevers. This paper describes a variant of the technique, scanning laser Doppler vibrometry, optimized for colloid probe cantilevers and capable of spring constant calibration uncertainties near ±1%.

Transport is a critical factor in modern pork production and can seriously affect swine welfare. While previous research has explored thermal conditions during transport, the impact of extreme weather conditions on the trailer thermal environment under industry practices has not been well documented; and the critical factors impacting microclimate are not well understood. To assess the trailer microclimate during transport events, an instrumentation system was designed and installed at the central ceiling level, pig level and floor-level in each of six zones inside a commercial swine trailer. Transport environmental data from 34 monitoring trips (approximately 1-4 h in duration each) were collected from May, 2012, to February, 2013, with trailer management corresponding to the National Pork Board Transport Quality Assurance (TQA) guidelines in 31 of these trips. According to the TQA guidelines, for outdoor temperature ranging from 5 °C (40 °F) to 27 °C (80 °F), acceptable thermal conditions were observed based on the criteria that no more than 10% of the trip duration was above 35 °C (95 °F) or below 0 °C (32 °F). Recommended bedding, boarding and water application were sufficient in this range. Measurements support relaxing boarding guidelines for moderate outdoor conditions, as this did not result in less desirable conditions. Pigs experienced extended undesirable thermal conditions for outdoor temperatures above 27 °C (80 °F) or below 5 °C (40 °F), meriting a recommendation for further assessment of bedding, boarding and water application guidelines for extreme outdoor temperatures. An Emergency Livestock Weather Safety Index (LWSI) condition was observed inside the trailer when outdoor temperature exceeded 10 °C (50 °F); although the validity of LWSI to indicate heat stress for pigs during transport is not well established. Extreme pig surface temperatures in the rear and middle zones of the trailer were more frequently experienced than in the front zones, and the few observations of pigs dead or down upon arrival were noted in these zones. Observations indicate that arranging boarding placement may alter the ventilation patterns inside the trailer.

Image processing systems have been widely used in monitoring livestock for many applications, including identification, tracking, behavior analysis, occupancy rates, and activity calculations. The primary goal of this work was to quantify image processing performance when monitoring laying hens by comparing length of stay in each compartment as detected by the image processing system with the actual occurrences registered by human observations. In this work, an image processing system was implemented and evaluated for use in an environmental animal preference chamber to detect hen navigation between 4 compartments of the chamber. One camera was installed above each compartment to produce top-view images of the whole compartment. An ellipse-fitting model was applied to captured images to detect whether the hen was present in a compartment. During a choice-test study, mean ± SD success detection rates of 95.9 ± 2.6% were achieved when considering total duration of compartment occupancy. These results suggest that the image processing system is currently suitable for determining the response measures for assessing environmental choices. Moreover, the image processing system offered a comprehensive analysis of occupancy while substantially reducing data processing time compared with the time-intensive alternative of manual video analysis. The above technique was used to monitor ammonia aversion in the chamber. As a preliminary pilot study, different levels of ammonia were applied to different compartments while hens were allowed to navigate between compartments. Using the automated monitor tool to assess occupancy, a negative trend of compartment occupancy with ammonia level was revealed, though further examination is needed., (©2014 Poultry Science Association Inc.)

The intermittent contact resonance atomic force microscopy (ICR-AFM) mode proposed here is a new frequency modulation technique performed in scanning force controlled AFM modes like force volume or peak force tapping. It consists of tracking the change in the resonance frequency of an eigenmode of a driven AFM cantilever during scanning as the AFM probe intermittently contacts a surface at a controlled applied maximum force (setpoint). A high speed data capture was used during individual oscillations to obtain detailed contact stiffness-force curve measurements on a two-phase polystyrene/poly(methyl methacrylate) film with sub-micrometer size domains. Through a suitable normalization, the measurements were analyzed by linear fits to provide an improved quantitative characterization of these materials in terms of their elastic moduli and adhesive properties.

We established a four-biofilter setup to examine the effects of moisture content (MC) on biofilter performance, including NH3 removal and N2O generation. We hypothesized that MC increase can improve NH3 removal, stimulate N2O generation and alter the composition and function of microbial communities. We found that NH3 removal efficiency was greatly improved when MC increased from 35 to 55%, but further increasing MC to 63% did not help much; while N2O concentration was low at 35-55% MC, but dramatically increased at 63% MC. Decreasing MC from 63 to 55% restored N2O concentration. Examination of amoA communities using T-RFLP and real-time qPCR showed that the composition and abundance of ammonia oxidizers were not significantly changed in a "moisture disturbance-disturbance relief" process in which MC was increased from 55 to 63% and then reduced to 55%. This observation supported the changes of NH3 removal efficiency. The composition of nosZ community was altered at 63% MC and then was recovered at 55% MC, which indicates resilience to moisture disturbance. The abundance of nosZ community was negatively correlated with moisture content in this process, and the decreased nosZ abundance at 63% MC explained the observation of increased N2O concentration at that condition., (Copyright © 2014 Elsevier B.V. All rights reserved.)

There are many atomic force microscopy (AFM) applications that rely on quantifying the force between the AFM cantilever tip and the sample. The AFM does not explicitly measure force, however, so in such cases knowledge of the cantilever stiffness is required. In most cases, the forces of interest are very small, thus compliant cantilevers are used. A number of methods have been developed that are well suited to measuring low stiffness values. However, in some cases a cantilever with much greater stiffness is required. Thus, a direct, traceable method for calibrating very stiff (approximately 200 N/m) cantilevers is presented here. The method uses an instrumented and calibrated nanoindenter to determine the stiffness of a reference cantilever. This reference cantilever is then used to measure the stiffness of a number of AFM test cantilevers. This method is shown to have much smaller uncertainty than previously proposed methods. An example application to fracture testing of nanoscale silicon beam specimens is included.

Quantifying non-methane hydrocarbons (NMHC) from animal feeding operations (AFOs) is challenging due to the broad spectrum of compounds and the polar nature of the most abundant compounds. The purpose of this study was to determine the performance of commercial NMHC analyzers for measuring volatile organic compounds (VOCs) commonly emitted from AFOs. Three different NMHC analyzers were tested for response to laboratory generated VOCs, and two were tested in the field at a commercial poultry facility. The NMHC analyzers tested included gas chromatography/flame ionization detector (GC/FID), photoacoustic infrared (PA-IR) and photoionization detector (PID). The GC/FID NHHC analyzer was linear in response to nonpolar compounds, but detector response to polar oxygenated compounds were lower than expected due to poor peak shape on the column. The PA-IR NMHC instrument responded well to the calibration standard (propane), methanol, and acetone, but it performed poorly with larger alcohols and ketones and acetonitrile. The PA-IR response varied between compounds in similar compound classes. The PID responded poorly to many of the most abundant VOCs at AFOs, and it underreported alcohols by > 70%. In the field monitoring study, total NMHC concentrations were calculated from sum total of VOC determined using EPA Methods TO-15 and TO-17 with GC-MS compared to results from NMHC analyzers. NMHC GC/FID values were greater than the values calculated from the individual compound measurements. This indicated the presence of small hydrocarbons not measured with TO-15 or TO-17 such as propane. The PA-IR response was variable, but it was always lower than the GC/FID response. Results suggest that improved approaches are needed to accurately determine the VOC profile and NMHC emission rates from AFOs.

Mitigation of bioaerosol emissions from concentrated animal feeding operations (CAFOs) demands knowledge of bioaerosol concentrations feeding into an end-of-pipe air treatment process. The aim of this preliminary study was to measure total endotoxin and (1 --> 3)-beta-glucan concentrations at the air exhaust of 18 commercial CAFOs and to examine their variability with animal operation type (swine farrowing, swine gestation, swine weaning, swine finishing, manure belt laying hen, and tom turkey) and season (cold, mild, and hot). The measured airborne concentrations of total endotoxin ranged from 98 to 23,157 endotoxin units (EU)/m3, and the airborne concentrations of total (1 --> 3)-beta-D-glucan ranged from 2.4 to 537.9 ng/m3. Animal operation type in this study had a significant effect on airborne concentrations of total endotoxin and (1 --> 3)-beta-D-glucan but no significant effect on their concentrations in total suspended particulate (TSP). Both endotoxin and (1 --> 3)-beta-D-glucan attained their highest airborne concentrations in visited tom turkey buildings. Comparatively, season had no significant effect on airborne concentrations of total endotoxin or (1 --> 3)-beta-D-glucan. Endotoxin and (1 --> 3)-beta-glucan concentrations in TSP dust appeared to increase as the weather became warmer, and this seasonal effect was significant in swine buildings. Elevated indoor temperatures in the hot season were considered to facilitate the growth and propagation of bacteria and fungi, thus leading to higher biocomponent concentrations in TSP.

Mode correction factors (MCFs) represent a significant adjustment to the spring constant values measured using the thermal cantilever calibration method. Usually, the ideal factor of 0.971 for a tipless rectangular cantilever is used, which adjusts the value by 3% for the first flexural mode. An experimental method for determining MCFs has been developed that relies on measuring the areas under the first few resonance peaks for the flexural mode type. Using this method, it has been shown that MCFs for the first flexural mode of commercially available atomic force microscope cantilevers actually vary from 0.95 to 1.0, depending on the shape and end mass of the cantilever. Triangular shaped cantilevers tend to lower MCFs with tipless versions providing the lowest values. Added masses (including tips) tend to increase the first flexural mode's MCF to higher values with large colloid probes at the high extreme. Using this understanding and applying it to the recently developed laser Doppler vibrometry thermal calibration method it is now possible to achieve very accurate and precise cantilever spring constant calibrations (uncertainties close to ±1%) with commonly available commercial cantilevers such as tipped rectangular and triangular cantilevers, and colloid probes.

Accurate cantilever spring constants are important in atomic force microscopy both in control of sensitive imaging and to provide correct nanomechanical property measurements. Conventional atomic force microscope (AFM) spring constant calibration techniques are usually performed in an AFM. They rely on significant handling and often require touching the cantilever probe tip to a surface to calibrate the optical lever sensitivity of the configuration. This can damage the tip. The thermal calibration technique developed for laser Doppler vibrometry (LDV) can be used to calibrate cantilevers without handling or touching the tip to a surface. Both flexural and torsional spring constants can be measured. Using both Euler-Bernoulli modeling and an SI traceable electrostatic force balance technique as a comparison we demonstrate that the LDV thermal technique is capable of providing rapid calibrations with a combination of ease, accuracy and precision beyond anything previously available.

Gas-phase compost biofilters are extensively used in concentrated animal feeding operations to remove odors and, in some cases, ammonia from air sources. The expected biochemical pathway for these predominantly aerobic systems is nitrification. However, non-uniform media with low oxygen levels can shift biofilter microbial pathways to denitrification, a source of greenhouse gases. Several factors contribute to the formation of anoxic/anaerobic zones: media aging, media and particle structure, air velocity distribution, compaction, biofilm thickness, and moisture content (MC) distribution. The present work studies the effects of media moisture conditions on ammonia (NH(3)) removal and greenhouse gas generation (nitrous oxide, N(2)O and methane, CH(4)) for gas-phase compost biofilters subject to a 100-day controlled drying process. Continuous recordings were made for the three gases and water vapor (2.21-h sampling cycle, each cycle consisted of three gas species, and water vapor, for a total of 10,050 data points). Media moisture conditions were classified into three corresponding media drying rate (DR) stages: Constant DR (wetter media), falling DR, and stable-dry system. The first-half of the constant DR period (0-750 h; MC=65-52%, w.b.) facilitated high NH(3) removal rates, but higher N(2)O generation and no CH(4) generation. At the drier stages of the constant DR (750-950 h; MC=52-48%, w.b.) NH(3) removal remained high but N(2)O net generation decreased to near zero. In the falling DR stage (1200-1480 h; MC=44-13%) N(2)O generation decreased, CH(4) increased, and NH(3) was no longer removed. No ammonia removal or greenhouse gas generation was observed in the stable-dry system (1500-2500 h; MC=13%). These results indicate that media should remain toward the drier region of the constant DR (in close proximity to the falling DR stage; MC=50%, approx.), to maintain high levels of NH(3) removal, reduced levels of N(2)O generation, and nullify levels of CH(4) generation., (Copyright © 2012 Elsevier Ltd. All rights reserved.)

Two independent lateral-force calibration methods for the atomic force microscope (AFM)--the hammerhead (HH) technique and the diamagnetic lateral force calibrator (D-LFC)--are systematically compared and found to agree to within 5 % or less, but with precision limited to about 15 %, using four different tee-shaped HH reference probes. The limitations of each method, both of which offer independent yet feasible paths toward traceable accuracy, are discussed and investigated. We find that stiff cantilevers may produce inconsistent D-LFC values through the application of excessively high normal loads. In addition, D-LFC results vary when the method is implemented using different modes of AFM feedback control, constant height and constant force modes, where the latter is more consistent with the HH method and closer to typical experimental conditions. Specifically, for the D-LFC apparatus used here, calibration in constant height mode introduced errors up to 14 %. In constant force mode using a relatively stiff cantilever, we observed an ≈ 4 % systematic error per μN of applied load for loads ≤ 1 μN. The issue of excessive load typically emerges for cantilevers whose flexural spring constant is large compared with the normal spring constant of the D-LFC setup (such that relatively small cantilever flexural displacements produce relatively large loads). Overall, the HH method carries a larger uncertainty, which is dominated by uncertainty in measurement of the flexural spring constant of the HH cantilever as well as in the effective length dimension of the cantilever probe. The D-LFC method relies on fewer parameters and thus has fewer uncertainties associated with it. We thus show that it is the preferred method of the two, as long as care is taken to perform the calibration in constant force mode with low applied loads.