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HP-INNOWAX capillary column (30 m x 0.25 mm x 0.5 µm) was adopted, with N2 as carrier gas, with the flow rate of 0.7 mL ⋅ min-1 to establish a GC method for the determination of eucalyptol, camphor and borneol in essential oil from Artemisia argyi folium. The linear range of eucalyptol, camphor and borneol was 48.78 ~ 731.6 µg ⋅ mL-1 (r=1.000 0), 10.00 ~ 150.0 µg ⋅ mL-1 (r = 1.000 0) and 11.16 ~ 167.4 µg ⋅ mL-1 (r = 1.000 0), respectively. The average recovery was 99.42% (RSD: 0.89%), 99.76% (RSD: 0.91%) and 101.42% (RSD: 1.44%), respectively. The method is simple, stable, accurate and repeatable, and can be used to determine the content of eucalyptol, camphor and borneol in essential oil from Artemisia argyi folium. [ABSTRACT FROM AUTHOR]

The leaf of Elaeagnus lanceolata and Elaeagnus henryi as well as Elaeagnus pungens has been documented as an effective herb for the treatment of asthma and chronic bronchitis in traditional clinical medicine. This study was aimed at evaluating the antiasthmatic, antitussive, and expectorant activities of the water extracts from the three plants in vivo and analyzing their chemical components by HPLC-DAD. At the medium and high doses, the water extracts of three Elaeagnus leaves significantly prolonged the preconvulsive time (P < 0.01) in guinea pigs, lengthened the latent period of cough (P < 0.01) and decreased the cough frequency caused by aqueous ammonia in mice (P < 0.01), and enhanced tracheal phenol red output in mice (P < 0.01). There were no significant differences in the pharmacological actions between the three Elaeagnus leaves. Moreover, there was more similarity on overlap peaks in the range of retention time from 10 to 40 min by HPLC and many peaks that belonged to flavonoids compounds. It suggested that the main constituents of the three Elaeagnus leaves were flavonoid for the pharmacological activities. These effects were the important evidence for the traditional use of E. henryi leaf and E. lanceolata leaf as well as E. pungens to treat asthma and chronic bronchitis.