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192 results on '"Gelderblom, Hans R."'

3. Olfactory transmucosal SARS-CoV-2 invasion as a port of central nervous system entry in individuals with COVID-19

Author

Meinhardt, Jenny, Radke, Josefine, Dittmayer, Carsten, Franz, Jonas, Thomas, Carolina, Mothes, Ronja, Laue, Michael, Schneider, Julia, Brünink, Sebastian, Greuel, Selina, Lehmann, Malte, Hassan, Olga, Aschman, Tom, Schumann, Elisa, Chua, Robert Lorenz, Conrad, Christian, Eils, Roland, Stenzel, Werner, Windgassen, Marc, Rößler, Larissa, Goebel, Hans-Hilmar, Gelderblom, Hans R., Martin, Hubert, Nitsche, Andreas, Schulz-Schaeffer, Walter J., Hakroush, Samy, Winkler, Martin S., Tampe, Björn, Scheibe, Franziska, Körtvélyessy, Péter, Reinhold, Dirk, Siegmund, Britta, Kühl, Anja A., Elezkurtaj, Sefer, Horst, David, Oesterhelweg, Lars, Tsokos, Michael, Ingold-Heppner, Barbara, Stadelmann, Christine, Drosten, Christian, Corman, Victor Max, Radbruch, Helena, and Heppner, Frank L.

Published
2021
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13. Electron microscopy for rapid diagnosis of infectious agents in emergent situations (1). (Synopses)

Author

Hazelton, Paul R. and Gelderblom, Hans R.

Subjects
Viruses -- Methods, Viruses -- Identification and classification, Virus diseases -- Methods, Virus diseases -- Diagnosis, Electron microscopy -- Usage
Abstract

Diagnostic electron microscopy has two advantages over enzyme-linked immunosorbent assay and nucleic acid amplification tests. After a simple and fast negative stain preparation, the undirected, "open view" of electron microscopy [...]

Published
2003

15. Olfactory transmucosal SARS-CoV-2 invasion as a port of central nervous system entry in individuals with COVID-19

Author

Meinhardt, Jenny, primary, Radke, Josefine, additional, Dittmayer, Carsten, additional, Franz, Jonas, additional, Thomas, Carolina, additional, Mothes, Ronja, additional, Laue, Michael, additional, Schneider, Julia, additional, Brünink, Sebastian, additional, Greuel, Selina, additional, Lehmann, Malte, additional, Hassan, Olga, additional, Aschman, Tom, additional, Schumann, Elisa, additional, Chua, Robert Lorenz, additional, Conrad, Christian, additional, Eils, Roland, additional, Stenzel, Werner, additional, Windgassen, Marc, additional, Rößler, Larissa, additional, Goebel, Hans-Hilmar, additional, Gelderblom, Hans R., additional, Martin, Hubert, additional, Nitsche, Andreas, additional, Schulz-Schaeffer, Walter J., additional, Hakroush, Samy, additional, Winkler, Martin S., additional, Tampe, Björn, additional, Scheibe, Franziska, additional, Körtvélyessy, Péter, additional, Reinhold, Dirk, additional, Siegmund, Britta, additional, Kühl, Anja A., additional, Elezkurtaj, Sefer, additional, Horst, David, additional, Oesterhelweg, Lars, additional, Tsokos, Michael, additional, Ingold-Heppner, Barbara, additional, Stadelmann, Christine, additional, Drosten, Christian, additional, Corman, Victor Max, additional, Radbruch, Helena, additional, and Heppner, Frank L., additional

Published
2020
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18. Bacteriophage diversity in the North Sea

Author

Wichels, Antje, Biel, Stefan S., Gelderblom, Hans R., Brinkhoff, Thorsten, Muyzer, Gerard, and Schutt, Christian

Subjects
North Sea -- Natural history, Bacteriophages -- Research, Electron microscopy -- Usage, Hybridization -- Usage, Host-bacteria relationships -- Research, Biological sciences
Abstract

Eighty-five phages were collected from an area near, Helgoland, a German island in the North Sea, to examine 22 phage-host systems in aquatic environments and genetic relationships of the phages. Electron microscopy, DNA-DNA hybridization and host range studies were used to determine the relationships between phages. Results show that there is no DNA homology between phages and that the 22 bacteriophages came from 13 different species.

Published
1998

21. An amino-terminal segment of hantavirus nucleocapsid protein presented on hepatitis B virus core particles induces a strong and highly cross-reactive antibody response in mice

Author

Geldmacher, Astrid, Skrastina, Dace, Petrovskis, Ivars, Borisova, Galina, Berriman, John A, Roseman, Alan M, Crowther, R.Anthony, Fischer, Jan, Musema, Shamil, Gelderblom, Hans R, Lundkvist, Åke, Renhofa, Regina, Ose, Velta, Krüger, Detlev H, Pumpens, Paul, and Ulrich, Rainer

Published
2004
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25. Genetical and functional investigation of fliC genes encoding flagellar serotype H4 in wildtype strains of Escherichia coli and in a laboratory E. coli K-12 strain expressing flagellar antigen type H48

Author

Schaudinn Christoph, Kaulfuss Stefan, Zimmermann Sonja, Strauch Eckhard, Beutin Lothar, Männel Andrea, and Gelderblom Hans R

Subjects
Microbiology, QR1-502
Abstract

Abstract Background Serotyping of O-(lipopolysaccharide) and H-(flagellar) antigens is a wideley used method for identification of pathogenic strains and clones of Escherichia coli. At present, 176 O- and 53 H-antigens are described for E. coli which occur in different combinations in the strains. The flagellar antigen H4 is widely present in E. coli strains of different O-serotypes and pathotypes and we have investigated the genetic relationship between H4 encoding fliC genes by PCR, nucleotide sequencing and expression studies. Results The complete nucleotide sequence of fliC genes present in E. coli reference strains U9-41 (O2:K1:H4) and P12b (O15:H17) was determined and both were found 99.3% (1043 of 1050 nucleotides) identical in their coding sequence. A PCR/RFLP protocol was developed for typing of fliC-H4 strains and 88 E. coli strains reacting with H4 antiserum were investigated. Nucleotide sequencing of complete fliC genes of six E. coli strains which were selected based on serum agglutination titers, fliC-PCR genotyping and reference data revealed 96.6 to 100% identity on the amino acid level. The functional expression of flagellin encoded by fliC-H4 from strain U9-41 and from our strain P12b which is an H4 expressing variant type was investigated in the E. coli K-12 strain JM109 which encodes flagellar type H48. The fliC recombinant plasmid carrying JM109 strains reacted with both H4 and H48 specific antisera whereas JM109 reacted only with the H48 antiserum. By immunoelectron microscopy, we could show that the flagella made by the fliC-H4 recombinant plasmid carrying strain are constituted of H48 and H4 flagellins which are co-assembled into functional flagella. Conclusion The flagellar serotype H4 is encoded by closely related fliC genes present in serologically different types of E. coli strainswhich were isolated at different time periods and geographical locations. Our expression studies show for the first time, that flagellins of different molecular weigth are functionally expressed and coassembled in the same flagellar filament in E. coli.

Published
2005
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30. External Quality Assurance (EQA) in Diagnostic Electron Microscopy (DEM) of Infectious Diseases: Aims and Roots, Results and Perspectives

Author

Gelderblom, Hans R., Möller, Lars, and Laue, Michael

Subjects
610 Medizin, ddc:610
Abstract

During the 1970s–80s, besides virus culture diagnostic electron microscopy (DEM)1 became a prime technique in clinical virology and laboratory diagnosis of infectious diseases. Today DEM appears on the retreat for several reasons, e.g., routine DEM is often replaced by molecular, highly sensitive and high throughput methods. Nevertheless, DEM needs to be kept in the diagnostic repertoire to cope with emerging infections, clinical emergencies, quality control (QC), and potential bioterrorism (Biel and Gelderblom, 1999 a; Curry et al., 2006; Gelderblom, 2003 a, b; Gelderblom, 2012; Gentile and Gelderblom, 2005; Hazelton and Gelderblom, 2003; Miller, 2003). As the consequences of an infection or an attack with biological weapons are amenable to intervention only for some hours, the speed of DEM is an essential advantage (Gentile and Gelderblom, 2014; Biel and Madeley, 2001; Madeley, 2003). Speed and “open view” of DEM allow an early and/or a differential diagnosis and also the diagnosis of multiple and/or unexpected infections down to the family level (Almeida, 1963, 1980; Hazelton and Gelderblom, 2003; Goldsmith and Miller, 2009), occasionally also beyond the family level (Goldsmith, 2014). As the quality of DEM depends to a great extent on expertise and personal skills, it is essential to run DEM in a quality controlled way and supported by continuous practicing and education (Gelderblom, 2001, PDF). After a brief description of historic aspects of QC in DEM of infectious diseases, of its principles and aims, this review focuses on the external quality assurance scheme in DEM (EQA-EMV) and the system of meetings and DEM lab courses run by the Robert Koch Institute (RKI) in Berlin since 1993. This field is depicted on the wider background of the history of the EM group in Virology, founded in 1971 at the RKI.

Published
2016

31. Optische und Elektronenmikroskopische Detektion – Erregerschnelldiagnostik, hochauflösende Lichtmikroskopie und Live-Cell-Imaging

Author

Brandenburg, Börries, Bujny, Miriam V., and Gelderblom, Hans R.

Subjects
Article
Abstract

Zusammenfassung Die Kombination aus Zellbiologie und Mikrobiologie hat mit der Zellulären Mikrobiologie eine neue Disziplin geschaffen, deren primäres Ziel die Erforschung des Zusammenspiels von Wirt und Erregern auf der Ebene des Gewebes, der Zelle und letztlich von einzelnen Molekülen ist. Die Entschlüsselung dieser dynamischen Interaktionen erlaubt ein tiefgreifendes Verständnis von Infektionsmechanismen sowie zellulärer Logistik und bereitet damit den Weg für die Entwicklung wirkungsvoller Therapeutika.

Published
2011

32. Human monoclonal antibody as prophylaxis for SARS coronavirus infection in ferrets

Author

Meulen, Jan ter, Bakker, Alexander B.H., van den Brink, Edward N., Weverling, Gerrit J., Martina, Byron EE, Haagmans, Bart L, Kuiken, Thijs, de Kruif, John, Preiser, Wolfgang, Spaan, Willy, Gelderblom, Hans R., Goudsmit, Jaap, and Osterhaus, Albert D.M.E.

Subjects
Monoclonal antibodies -- Research, Coronavirus infections -- Research, Severe acute respiratory syndrome -- Research
Published
2004

33. Specimen Collection for Electron Microscopy

Author

Gelderblom, Hans R. and Hazelton, Paul R.

Subjects
Viral antigens -- Diagnosis, Diagnosis, Electron microscopic -- Methods
Abstract

To the Editor: As virologists whose specialties include diagnostic electron microscopy (EM), we read with interest the discussion on bioterrorism scenarios (1,2) and the subsequent note by Marshall and Catton [...]

Published
2000

44. Molecular and Biological Characterization of Human Monoclonal Antibodies Binding to the Spike and Nucleocapsid Proteins of Severe Acute Respiratory Syndrome Coronavirus

Author

van den Brink, Edward N., primary, ter Meulen, Jan, additional, Cox, Freek, additional, Jongeneelen, Mandy A. C., additional, Thijsse, Alexandra, additional, Throsby, Mark, additional, Marissen, Wilfred E., additional, Rood, Pauline M. L., additional, Bakker, Alexander B. H., additional, Gelderblom, Hans R., additional, Martina, Byron E., additional, Osterhaus, Albert D. M. E., additional, Preiser, Wolfgang, additional, Doerr, Hans Wilhelm, additional, de Kruif, John, additional, and Goudsmit, Jaap, additional

Published
2005
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