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Native yeasts produce most peptides and amino acids from cheese whey fermentation. The research aimed to determine the protein, peptides, and amino acid profiles regarding whey fermentation by spontaneous fermentation and re-inoculation by native yeasts as a basis for determining potential yeasts in amino acid production. The selected native yeasts were identified through genome-based analysis on the DNA base sequence of the Internal Transcribed Spacer (ITS) region. The molecular identification shows that isolate CT1 is 95.62% comparable to Candida tropicalis strain VIT-NN02, CT2 is 98.85% same to stain NB5Y, and CT3 is 99.42% similar to GT 6. Whey fermentation was conducted by inoculating 3% each native C. tropicalis and without inoculation for spontaneous fermentation, then incubated for 48 hours at ambient temperature (26 ± 2°C). Unlike the other samples, the spontaneously fermented mozzarella whey was incubated without inoculation after cheese separation. After 48 hours, fermented whey was kept at 4°C for examination. The Kjeldahl method evaluated protein concentration, Folin-Ciocalteau determined peptide levels, and HPLC determined amino acid composition. C.tropicalis was identified as cheese whey native yeasts, and all treatments decreased protein and peptides while increased the total amino acid includes every single amino acid value except histidine during isolate CT1 whey fermentation. Candida tropicalis CT2 shown as potential isolate for the amino acid production from whey, with the protein contents of 0.846 ± 0.151%, protein decreases of 0.384 ± 0.186, peptide levels of 264.75 ± 40.40ppm, peptide decreases of 99.87 ± 37.96 and the highest total amino acid of 6149.56ppm.

This study used tofu wastewater as medium to produce mannoprotein from Candida tropicalis (MCT), Pichia norvegensis (MPN), Aspergillus awamori (MAA), and Rhizopus oryzae (MRO). This study aimed to determine the optimum growth of yeast and mold in tofu wastewater as a medium for mannoprotein production. The purity was determined using FTIR and solid-state NMR spectroscopy. The structural differences were determined using scanning electron microscopy (SEM), and the antioxidant activity was measured using DPPH radical scavenging activity. The highest yield of extract was obtained upon the use of MPN (28.09 ± 6.4%, w/w), followed by MCT (18.73 ± 5.5%, w/w), MRO (2.75 ± 1.7%, w/w), and MAA (2.54 ± 1.7%, w/w). Moreover, FTIR spectroscopy confirmed that MCT, MPN, MAA, and MRO were typical mannoproteins with absorption band at 824, 824, 818, and 812 cm−1, respectively. While the1H NMR spectra revealed the presence of protons at 5.11–5.46 ppm which confirmed the presence of α-(1,2)-mannoprotein for MCT, α-(1,6)(1,2)-mannoprotein for MPN, and α-(1,6)-mannoprotein for MAA, and MRO. Mold-based mannoprotein has a smaller particle size than yeast-based mannoprotein, furthermore, at 4000 ppm, MCT had the greatest DPPH-free radical inhibition value of 49.89%, followed by MRO (44.34%), MAA (40.81%), and MPN (28.54%).

This study aims to determine the applicability of tofu wastewater as a medium for yeast growth and to determine the optimal growth time of yeast to produce β-glucans, as well as the yield and characteristics of β-glucans extract. Pichia norvegensis and Candida tropicalis cell walls were extracted for β-glucan. FTIR, solid-state NMR, and SEM were utilized to investigate purity, structural differences, and antioxidant activity. Candida tropicalis (BCT) yielded 3.20 % crude β-glucan, while Pichia norvegiensis NYI (BPN) yielded 2.10 %. FTIR showed β-glucan impurities in mannoprotein-chitosan combinations. BPN and BCT have β-glucan bands at 1075, 1041, and 894 cm−1. The impurity of both samples was confirmed by NMR spectra, which showed that the broad signal of BPN and BCT chemical shift at 4.803–4.587 ppm and at 4.764–4.541 ppm, respectively, corresponded to l β-1,3-glucosidic and β-1,6-glucosidic linkage, with some unusual anomeric resonance at 5.094–5.024 ppm by SEM, BPN and BCT were porous, sheet-like, and fibrous. BPN had 20.07 % DPPH radical scavenging activity, while BCT had 36.01 %.

Terasi, a traditional Indonesian seafood product made from shrimp, undergoes fermentation facilitated by a consortium of microorganisms, including Lactic Acid Bacteria (LAB) and yeast, which contribute to its distinctive umami flavor. This study investigates the microbial dynamics and production of key metabolites, including γ-aminobutyric acid (GABA), during terasi fermentation. Total Plate Count (TPC) and High-Performance Liquid Chromatography (HPLC) were used to monitor changes in glutamate and GABA levels, with glutamate increasing from 105.18 mg/mL on day 3–139.19 mg/mL on day 14, and GABA rising from 90.49 mg/mL to 106.98 mg/mL over the same period. Metagenomic analysis using high-throughput sequencing of bacterial 16 S rRNA identified Firmicutes, Proteobacteria, Actinobacteria, and Bacteroidota as dominant phyla. While LAB populations remained relatively stable, yeast became detectable from day 4. Notably, core bacterial genera such as Vibrio, Macrococcus, Staphylococcus, Exiguobacterium, Jeotgalicoccus, Prevotella, Salinicoccus, Bacillus, Pseudarthrobacter, and Vagococcus were highly abundant and played significant roles in GABA production, likely due to their glutamate decarboxylase activity. These findings reveal a clear correlation between microbial succession and metabolite production, offering valuable insights into the fermentation process of terasi. This study enhances the understanding of traditional food fermentation and presents opportunities to optimize beneficial compounds in terasi products.

Whey is an excellent source of proteins, with a high biological value and essential elements in addition to the proteins it contains. Additionally, it possesses a number of characteristics that are exclusive to it. Whey has been “rediscovered” in recent years and is now being used in a wider variety of food recipes as a result of the features discussed here in the present review paper. It’s possible that using whey instead of the “nutrient goldmine” will be more “economical.” Consumers who are educated on the role that whey plays in human nutrition and are aware of its significance are more likely to be interested in purchasing food products that include whey. Due to the nutritional and functional features that whey possesses, it has been included into a wide variety of food compositions, such as dairy-based products, biofunctional components, confectionary products, and beverages that are both refreshing and nutritious. In this review, several types of food products and beverages containing whey that have been produced so far by researchers from all over the world are described, along with their processing characteristics, sensory attributes, and commercial potential. Moreover, this paper delves into the potential applications of probiotic and functional whey-based products within the realms of biofunctional and therapeutic relevance.

Soy products are a primary source of isoflavones, but many naturally occurring isoflavones are glycosides with low bioavailability. β-Glucosidase hydrolyzes the β-D-glucoside bond, releasing the aglycone form of isoflavones, which are more efficiently absorbed and offer enhanced health benefits, such as anti-inflammatory and anticancer effects. Microorganisms play a critical role in this biotransformation during fermentation by producing β-glucosidase. This review summarizes the microorganisms involved in converting isoflavone glycosides into aglycones during the fermentation of Indonesian traditional foods. The process, influenced by heritage-rich methods, determines the efficiency of aglycone production. Various unexplored microorganisms in Indonesian soybean-based fermented foods, such as tempeh, oncom, kecap, and tauco, produce β-glucosidase. These findings highlight the importance of optimizing traditional fermentation methods to maximize the health benefits of soy products.

Modern cancer diagnostics and treatment options have greatly improved survival rates; the illness remains a major cause of mortality worldwide. Current treatments for cancer, such as chemotherapy, are not cancer-specific and may cause harm to healthy cells; therefore, it is imperative that new drugs for cancer be developed that are both safe and effective. It has been found that lactic acid bacteria (LAB) have the potential to produce bacteriocins, which could potentially offer a promising alternative for cancer treatment. They have been shown in several studies to be effective against cancer cells while having no effect on healthy cells. More research is needed to fully understand the potential of LAB bacteriocins as anti-cancer medicines, to find the appropriate dose and delivery route, and to conduct clinical trials to evaluate the effectiveness and safety of the products in human patients, as is suggested by this work. Furthermore, LAB bacteriocins may evolve into a significant new class of anti-cancer drugs and food products. Patients with cancer may have a safe and effective alternative treatment option in the form of anti-cancer foods and drugs. Therefore, the aim of this study is to provide an in-depth analysis of the recent breakthroughs and potential future technical advancements of significant bacteriocins that are produced by LAB, how these bacteriocins function, and how these bacteriocins may be utilized as an anti-cancer agent. In addition, the current analysis emphasizes the significant constraints and boundaries that bacteriocins face when they are used as an anti-cancer factor.

Decommissioning of nuclear facilities can be performed in stages. One of the stages and processes in decontamination is the decontamination process before dismantling or facility area recovery activities. Decontamination can be performed using various methods, primarily physical and chemical. One chemical method involves using a gel made of polymers for decontamination. In this study, a gel consisting of a mixture of 15 g polyvinyl alcohol (PVA), 15 mL of glycerol, and 2 g Na-EDTA was dissolved in 100 mL. The three materials were dissolved in hot conditions until they dissolved, and a gel was formed. The formed gel was applied to the material contaminated by Co-60 with a radioactivity of 81 µCi, as much as 5 µL. The decontamination radioactive efficiency test results range from 53% to 98%, with the highest decontamination efficiency observed on glass media. This study also showed that higher EDTA concentrations can increase the ability of the PVA-glycerol gel to absorb and bind Co. This study also found that decontamination efficiency was influenced by the type of contaminated material and the concentration of EDTA. It can be concluded that gels with a composition of PVA, glycerol, and EDTA can reduce the level of contamination on the surface of materials made of glass, ceramics, and metal plates.

Background and Aim: Investigation of the zoonotic potential of quail-derived food items, including eggs and meat, and their consequential impact on the health of the general population is insufficient. The present study provides a comprehensive overview of the potential hazards associated with consuming quail eggs and meat products with a One Health approach based on the existing body of knowledge derived from multi-disciplinary studies. Materials and Methods: A total of 37 studies from 2004 to 2021 were procured from four distinct databases following a two-stage screening process involving practical and methodological screening. The utilization of a descriptive qualitative method with a meta-aggregation approach was employed to scrutinize these studies, leading to a definitive conclusion regarding the risks of foodborne diseases associated with the consumption of quail meat products. Results: A total of 7555 studies were retrieved and 146 were qualified based on the predetermined criteria. Of 146 studies, 90 studies were eliminated based on duplication screening. Of the 90, 37 were determined to be related to the aim of this research. Conclusion: The consumption of quail eggs and meat products poses a significant risk for foodborne diseases, with potentially greater ramifications than currently recognized, particularly in the areas of food safety, public health, conservation, and the economy.

Chocolate biscuits are one type of biscuit that contains antioxidants, albeit in small amounts. Incorporating functional compounds into the product is an option for enhancing its quality. This study investigated chocolate biscuits’ physicochemical, antioxidant, and sensory properties incorporated with encapsulated mangosteen peel extract in percentages of 1%, 3%, 5%, and 7%. The increase in mangosteen peel extract microcapsules significantly affected the total value of polyphenols, flavonoids, peroxide number, color, and texture profile. But, there were no significant differences in the organoleptic characteristics of color, aroma, taste, texture, and overall acceptance. Total polyphenols, flavonoids, texture profile, a* and b* values, and browning index were all positively correlated with increasing the concentration of encapsulated in the formula. However, there was a negative correlation between antioxidant activity, peroxide number, and L*. It obtained the best recipe for its physicochemical, organoleptic, and antioxidant properties at 5%. Overall, the panelists approved the chocolate biscuits with mangosteen peel extract microcapsules. This product’s development is an alternative to antioxidant-rich functional foods.

Background: Black garlic is one of the functional food products made from garlic which is processed through aging to improve sensory value and nutritional quality. Aging conditions has a major impact on the psychochemical and functional properties changes of black garlic which is closely related to organosulfur compounds and polysaccharides as the largest component in garlic. Scope and approach: The method used in this research is a systematic review with the aim of research to determine the relationship between reactions during aging and changes in organosulfur, polysaccharides and non-enzymatic browning product compounds as well as the function of black garlic by focusing on certain aspects of aging including temperature, humidity, time, microorganism activity, and pre-treatment application. Key findings and conclusions: Maillard reaction and polysaccharide degradation are still be the dominant reactions and play an important role in black garlic production. High hydrostatic pressure pretreatment could maintains the quality of black garlic so that the black garlic has the same taste characteristics as black garlic in general. Antioxidant properties in black garlic shown increase during thermal treatment. In addition, it is known that the activity of microorganisms plays a role and being potential to increase the quality value of black garlic as well as the antimicrobial activity.

Pomegranate peels (PGPs) are known to have the potential as antibacterial not only from their nutrient content but also the microflora. The activities might be caused by the existence of indigenous yeast that can be utilized to inhibit the growth of pathogenic bacteria. This study aims to identify antibacterial and antioxidant activity of indigenous yeast isolated from PGP. The research was conducted by experimental methods and followed by descriptive analysis. The study was done by the isolation of indigenous yeast from PGPs, which was identified using the rRNA sequence analysis of internal transcribed spacer (ITS) region with the primers of ITS1 (5′-TCCGTAGGTGAACCTGCGG-3′) and ITS4 (5′-TCCTCCGCTTATTGATATGC-3′) and then compared with Basic Local Alignment Search Tools (BLAST) algorithm toward the GenBank. Antibacterial activities of indigenous yeast were tested with agar plug diffusion and time kill test toward Escherichia coli and Staphylococcus aureus. The yeast identification obtained two isolates similar to Hanseniaspora uvarum CBS 314 and two isolates of Pichia kudriavzevii ATCC 6258 which have antibacterial activity against E. coli and S. aureus. P. kudriavzevii PGP D4 have best antimicrobial activities with a strong activity against E. coli (±9 mm) and medium activity against S. aureus (±3.1 mm).

Spontaneous fermentation during black tea production involves several reactions, including the oxidation of phenolic compounds. This process has usually been studied without considering the potential involvement of indigenous tea microorganisms. This work utilised a metagenomic technique targeting bacterial 16S rRNA genes and evaluated the profile of phenolic compounds generated during the production of black tea. The resulting data were used to develop correlational and predictive functional analyses related to bacterial dynamics and the syntheses of various phenolic compounds. In particular, the genera Methylobacterium and Devosia were correlated with gallic acid and quercetin. Concurrently, the genera Sphingomonas, Chryseobacterium and Aureimonas were correlated with kaempferol, theaflavins, thearubigins and theabrownins. These results, supported by predicted functional analysis based on 16S rRNA genes associated with phenolic compounds, indicated that yfiH (polyphenol oxidase) and katG (catalase-peroxidase) are likely the dominant genes of the bacterial community involved in the black tea production process. This research suggests that bacteria could potentially contribute to the production process of black tea.

Cocoa shell is a waste of the cocoa processing industry, containing vitamin D, protein, fat, carbohydrates, and fiber as well as antioxidant compounds such as polyphenols. Fermentation and immersion processes are needed in post-harvest cocoa to create the distinctive taste and flavor of chocolate, as well as to produce cocoa bean shell powder, which is also good quality as a waste utilization. This study aims to determine the effect of fermentation and immersion processes on the antioxidant activity and characteristics of the cocoa bean shell powder (CBS). The research method used a completely randomized design (CRD) method with four treatments e.g. NFNI (Non Fermentation Non Immersion), NFI (Non Fermentation Immersion), FNI (Fermentation Non Immersion) and FI (Fermentation Immersion) with three replications. The results of the color parameters show that CBS has a yellow-red color with significantly different L* values following the order FNI > NFNI > FI > NFI and the a* and b* values following the order FNI < FI < NFNI < NFI. NFNI has the highest antioxidant activity with IC50 values of 12.93 ppm and total phenolic 4.33 g/100 g followed by NFI, FNI, and FI. The best treatment for texture, aroma and color parameters was FI with an average value above 7, and it also had overall appearance characteristics that were not significantly different from commercial cocoa powder.

Peningkatan literasi diharapkan dapat meningkatkan pengetahuan dan kemampuan santri di pondok pesantren Nurul Wafa Gunung Hideung, Kec. Singaparna, Kab, Tasikmalaya dalam memanfaatkan limbah pangan. Pendekatan yang dilakukan adalah sosialisasi melalui metode ceramah dan diskusi terkait pemanfaatan limbah pangan. Metode ini diharapkan tidak hanya meningkatkan literasi atas pemanfaatan limbah pangan tetapi juga mendorong para santri untuk lebih termotivasi dalam memanfaatkan limbah pangan karena dapat dilakukan secara mudah dan sederhana dengan sumber daya yang ada di sekitar mereka. Hasil menunjukkan bahwa ceramah dan pendampingan yang dilakukan melalui sosialisasi dan diskusi mampu meningkatkan literasi santri di Pondok Pesantren Nurul Wafa hingga 84% dengan peningkatan yang signifikan ditunjukkan dari rata-rata literasi sub materi yaitu mencapai 37% sementara untuk rata-rata literasi total menunjukkan peningkatan hingga 27%.

The development of honey and propolis as commercial commodities from Central Bangka Regency meet an obstacle considering the technology and marketing target. In order to develop the potential of honey and propolis as commercial products from Central Bangka Regency, identification, technical training and monitoring has been done as the methodology. The results shows identification of Honey and Propolis productivities showed that estimated 19,785 kg / year honey, 3,957 kg / year propolis, with total Kelulut colonies of more than 3957 colonies will resulted. Meanwhile the quality aspect of Kelulut honey found diastase enzyme activity of 1.68 DN, with propolis quality shown polyphenol levels reaching 2587.95 mg / kg with the characteristics that preferred by consumers and industry. Training and monitoring could increase the skill and knowledge of farmer groups

Buah mangga adalah produk yang mudah rusak sehingga perlu dilakukan pengolahan salah satunya menjadi buah kering. Pengeringan osmosis merupakan salah satu metode baru dalam pengeringan buah mangga. Pengeringan osmotik menggunakan larutan gula memungkinkan gula berperan sebagai penarik air sekaligus bahan pengawet alami sehingga dapat memperpanjang umur simpan buah mangga cengkir kering. Perhitungan umur simpan produk buah mangga kering ini dilakukan dengan pendekatan metode Arhhenius dengan pertumbuhan mikroorganisme sebagai titik kritis dengan model regresi linear y = -56599.4x + 13.67. Hasil penelitian menunjukkan bahwa umur simpan buah mangga kering adalah 4.47 bulan jika disimpan di suhu 30ºC.

The concept of a circular economy can be utilized in the process of starting a dairy cattle enterprise. A circular economy is not only a chance to lessen the amount of waste produced by dairy farms and cut down on the amount of pollution that is released into the environment, but also an attempt to maximize the number of advantages that are shared between the economy and the environment. A circular economy can be implemented at any level, from the micro-level (businesses and customers) to the meso-level (eco-industrial zones), and all the way up to the macro-level (city, province, or country). The identification of circular economy practices is possible through the use of Circular Performance Indicators (CPIs). The purpose of this research is to identify circular economy practices based on CPIs at the micro-level, with a focus on the Taruna Mukti Farmer Group in the Bandung Regency of West Java, Indonesia. Based on our research, it is found that the identified CPIs achieve an average score of 2.57, with an achievement level value of 85.5% (very good). The results of the MICMAC analysis show that the key indicator in the CPIs of livestock waste management in the Taruna Mukti Farmer Group is additional income/income from the processing of livestock waste (C1). There is a relationship between the management of livestock waste in the Taruna Mukti Farmer Group and the circular economy concept based on Circular Performance Indicators. Farmers see the aspect of economic profit (economic motive) as important in the management of livestock waste. Marketing and sales strategies will have a big influence on the system of converting livestock waste into organic fertilizer. The higher the sales volume, the higher the level of profit.

An entirely unknown species of coronavirus (COVID-19) outbreak occurred in December 2019. COVID-19 has already affected more than 180 million people causing ~3.91 million deaths globally till the end of June 2021. During this emergency, the food nutraceuticals can be a potential therapeutic candidate. Curcumin is the natural and safe bioactive compound of the turmeric (Curcuma longa L.) plant and is known to possess potent anti-microbial and immuno-modulatory properties. This review paper covers the various extraction and quantification techniques of curcumin and its usage to produce functional food. The potential of curcumin in boosting the immune system has also been explored. The review will help develop insight and new knowledge about curcumin's role as an immune-booster and therapeutic agent against COVID-19. The manuscript will also encourage and assist the scientists and researchers who have an association with drug development, pharmacology, functional foods, and nutraceuticals to develop curcumin-based formulations.

The study aims to determine the number of mass and antioxidant activity of β-glucan extracted from S.cerevisieae which grown on vegetable and fruit wastes. The method used is experimental with descriptive analysis which consisted of 3 treatments namely banana waste, papaya waste, and napa cabbage waste as fermentation medium, repeated thrice. Fermentation medium was made by mixing waste with water with the ratio of 1:2. Ten percent (w/v) of S.cerevisieae was inoculated and incubated for 48 hours, at 27 ℃. Extraction of β-glucan was carried out using acid-alkaline methods and antioxidant activity was tested by DPPH (2,2-Diphenyl-1-picrylhydrayl) method and the microstructure of β-glucan is determined by Scanning Electrone Microscope. The result showed that the best medium in producing β-glucan was papaya waste which resulting 19.094 g β-glucan mass, with radical scavenging activity of 20.71 % and globular diameter of 533 μm.

Whey is a by-product of cheese processing and is comprised of nearly 90% of the milk used. The protein content in cheese whey has the potential to create peptide and amino acids which have a functional effect in biological activity. Peptides and amino acids can be produced through fermentation with Candida tropicalis into native whey from cheese whey. The study aims to determine fermentation time in producing peptide and amino acid profiling in the fermentation of native cheese whey by Candida tropicalis. Cheese whey fermented with C. tropicalis was compared to a naturally fermented cheese whey as control at an ambient temperature for 48 hours. Peptide content identified by Folin–Ciocalteu methods and the amino acid profile is determined by high performance liquid chromatography (HPLC). Fermentation results showed that the maximum content of peptides needs a 24-hour fermentation in 10.42 ppm. Peptide content decreased with further fermentation caused by the degradation of peptides into amino acids. The amino acids that increased were aspartate, glutamate, threonine, valine, isoleucine, and lysine, while those that decreased were serine, histidine, glycine, arginine, alanine, tyrosine, and methionine.

This research aims to determine the effect of adding hull of mung bean sprouts extract to pH and characteristics of sensory yogurt probiotic. The research method used experimental with randomized block design. There were 4 additional treatments (1%, 2%, 3%, and 4% w /v hull of mung bean sprouts extract) with 3 replications. The data obtained will be tested further using the Duncan test. The addition hull of mung bean sprouts extract has a significant different effect on the pH and color characteristics of yogurt probiotic while giving no significant effect on the characteristics of aroma, taste, texture and overall acceptance. Addition hull of mung bean sprouts extract affects the pH of yogurt probiotic produced which is about 4.00 - 4.11. The color preferences of the yogurt probiotic are on the scale of dislike – really likes and preferences of the aroma, taste, texture and overall acceptance of yogurt probiotic are on the scale of usual - like. The conclusion of this study is that the addition hull of mung bean sprouts extract can prevent a decrease in pH, causing a decrease in the level of preference for color and yogurt probiotic that produced is acceptable to consumers.

Sodium is a necessary nutrient for regulating extracellular fluid and transferring molecules around cell membranes with essential functions. However, the prevalence of some diseases is related to unnecessary sodium intake. As a result, a particular problem for the food industry remains a matter of sodium content in foods. It is considered that customer acceptance is associated with salt perception dynamics related to the evolution of food production. It is a significant challenge and technique to minimize the salt content of various foods and provide replacement products with substantial reductions in salt levels. This review summarizes salt reduction strategies related to health problems based on traditional review methodology, with practical and methodological screening performed to determine the appropriate reference sources. Various technological (salt replacement, food reformulation, size and structural modifications, alternative processing, and crossmodal odor interaction) and behavioral strategies (memory process, gradual salt reduction, and swap) are identified in this work, including a deeper understanding of the principles for reducing sodium content in foods and their effect on food characteristics and potential opportunities for the food industry. Thereby, the food industry needs to find the proper combination of each strategy’s advantages and disadvantages to reduce salt consumption while maintaining product quality.

Antimicrobial composite edible film can be a solution for environmentally friendly food packaging, which can be made from fermented cheese whey containing an antimicrobial agent and cassava peel waste that contains starch. The research aims to determine the formulation of fermented cheese whey and cassava peel waste starch, resulting in an antimicrobial composite edible film with the best physical, mechanical, and water vapour permeability (WVP) properties, as well as with high antimicrobial activity. This research was conducted using experimental methods with nine composite edible film formulation treatments with three replications. Three variations in the fermented cheese whey and cassava peel starch ratio (v/v) (1:3, 1:1, 3:1) were combined with variations in the addition of glycerol (20%, 33%, 45%) (w/w) in the production of the composite edible film. Then, the physical characteristics such as elongation at break, tensile strength, WVP, colour, and antimicrobial effect of its film-forming solution were observed. The results showed that 24 h of whey fermentation with Candida tropicalis resulted in an 18.50 mm inhibition zone towards Pseudomonas aeruginosa. The best characteristic of the film was obtained from the formulation of a whey:starch ratio of 1:3 and 33% glycerol, which resulted in a thickness value of 0.21 mm, elongation at break of 19.62%, tensile strength of 0.81 N/mm2, WVP of 3.41 × 10−10·g/m·s·Pa at a relative humidity (RH) of 100%–35%, and WVP of 9.84 × 10−10·g/m·s·Pa at a RH of 75%–35%, with an antimicrobial activity towards P. aeruginosa of 5.11 mm.

Lignocellulose can be degraded by lignocellulolytic microorganisms such as moulds. The purpose of the study was to obtain the right type of moulds in degrading lignocellulose on the cocoa shell powder. The study used a completely randomized design method using four treatments of different types of mould (Trichoderma viride, Neurospora sitophila, Aspergillus niger, and Rhizopus oryzae) towards cocoa shell powder fermentation. Solid fermentation of cocoa shell powder was carried out for 5 days in an incubator with a temperature of 30°C for T. viride, N. sitophila, and R. oryzae, while A. niger of 35°C. The fermented substrate was then dried in a cabinet oven with a temperature of 50°C for 4 days. Tests of lignin, cellulose, and hemicellulose were performed towards the treatments by the Chesson method, while the moisture content test was performed using the AOAC method. Degradation of fermented cocoa shell powder has shown a significant effect on moisture, lignin, cellulose, and hemicellulose contents. Trichoderma viride resulted in the highest lignocellulose degradation compared with the other treatments. The percentage decrease of lignin content is up to 46.69 wt%; while cellulose of 22.59 wt%; and hemicellulose is about 19.41 wt% from the initial lignin weight.

The aim of the study was to isolate and identify the yeast isolated from Dangke and its potential as probiotics. The purified isolates obtained were identified based on observations of macroscopic characteristics of colonies and microscopic cells. The ability as a probiotic yeast is obtained by testing the resistance towards acid conditions, bile salt resistance test and aggregation ability test against pathogenic bacteria using Salmonella sp. The yeast isolates were identified using the RapID Yeast Plus System. The isolation result was obtained D.10.3.d isolate that identified as Candida guilliermondii which showed probiotic characteristic. The yeast colony is round, cream‑colored, smooth surfaces, low convex elevations and entire edges, capable of growing on mediums with the pH of 4, containing 1 % and 5 % of bile salts and having the ability to aggregate Salmonella sp. at 15, 60, and 180 minutes.

Yeast can be isolated from tofu wastewater and the cell wall in the form of β-glucan can act as a natural decontaminant agent. This study aimed to isolate and characterize native yeast from tofu wastewater, which can be extracted to obtain β-glucan and then identify the yeast and its β-glucan activity regarding antifungal ability against Aspergillus flavus and aflatoxin-reducing activity towards aflatoxin B1 (AFB1) and B2 (AFB2). Tofu wastewater native yeast was molecularly identified, and the growth observed based on optical density for 96 h and the pH also measured. β-glucan was extracted from native yeast cell walls with the acid-base method and then the inhibition activity towards A. flavus was tested using the well diffusion method and microscopic observation. AFB1 and AFB2 reduction were identified using HPLC LC-MS/MS. The results showed that the native yeast isolated was Pichia norvegensis with a β-glucan yield of 6.59%. Pichia norvegensis and its β-glucan showed an inhibition zone against Aspergillus flavus of 11.33 ± 4.93 and 7.33 ± 3.51 mm, respectively. Total aflatoxin-reducing activity was also shown by Pichia norvegensis of 26.85 ± 2.87%, and β-glucan of 27.30 ± 1.49%, while AFB1- and AFB2-reducing activity by Pichia norvegensis was 36.97 ± 3.07% and 27.13 ± 1.69%, and β-glucan was 27.13 ± 1.69% and 32.59 ± 4.20%, respectively.

The research aimed to identify indigenous yeast antibacterial activity from sapodilla fruit against Escherichia coli and Staphylococcus aureus, which conducted by experimental methods and followed by descriptive analysis. This study was done by the isolation of indigenous yeast, macroscopic and microscopic identification, yeast identification using RapID Yeast Plus System, antibacterial test by measuring the clear zone diameter, testing of pathogenic bacteria viability against indigenous yeast and identification of organic acid produced by yeast. The results of yeast isolation obtained 1 isolate (Saccharomyces cereviseae 1) from fruit and 3 isolates form sapodilla skin (S.cereviseae 2, Candida famata, and Pichia anomala) which had antibacterial activity against E. coli and S. aureus except C. famata isolates. Isolates with the largest antibacterial activity against E. coli and S. aureus based on the clear zone diameter were S. cerevisiae (2) isolates. The results of organic acid analysis by HPLC found that S.cerevisiae (2) isolate produced the highest organic acid namely acetic acid as much as 2.442 mg mL -1. Key words : antibacterial, organic acid, sapodilla fruit, yeast

Aims of the study was to determine the cost-benefit analysis in neufchatel whey bioconversion into rectified ethanol and organic liquid fertilizer. Bioconversion whey into rectified ethanol and organic liquid fertilizer has shown great potential as a way to reduce the pollution resulting from cheese-making process. Semi pilot scale experiment was done to ferment 5 L neufchatel whey using 5% K. lactis at 33°C for 24 h in semi anaerobic plastic container without agitation and then distilled into 96.2% purity. Data collected and analyzed descriptively related to benefit cost ratio/BCR, net present value/NPV and internal rate returns/IRR. The result showed that semi pilot scale bioconversion of neufchatel whey resulting in 106.42 ml rectified ethanol and 4404.22 ml distillery residue. Economic benefit could achieved by the support of distillery residue sales as organic liquid fertilizer.