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Objective To investigate the role and molecular mechanism of CXCL13 on the radioresistance of lung cancer. Methods Public database was used to analyze the expression levels of CXCL13 and CXCR5 in lung cancer patients, and the relation between the expression levels of CXCL13/CXCR5 and the prognosis of lung cancer patients who received radiotherapy only. Western blot was used to detect the effects of different concentrations of CXCL13 and Akt inhibitor LY294002 on Akt expression and activation in human lung cancer H1975 cells. CCK-8 method, flow cytometry and clone formation assay were used to analyze the effect of CXCL13 and LY294002 on the proliferation, apoptosis and cell clone formation ability after X ray radiation treatment, respectively. Results The expression levels of CXCL13 and its receptor CXCR5 in lung cancer patients were significantly higher than those in the normal group (P < 0.003). The high expression level of CXCL13 is closely related to the progression of postnatal survival (PPS) of lung cancer patients. H1975 cells treated with 100ng/ml CXCL13 showed significantly higher proliferation level (P=0.015) and clone formation ability (P=0.014), but lower apoptosis level (P=0.001) than the control group. 100ng/ml CXCL13 significantly activated Akt. After 8Gy X-ray radiation treatment, the cell proliferation level (P=0.019) and clone formation ability (P=0.001) in the 20μg/ml LY294002 combined with CXCL13 group were significantly lower than those in the CXCL13 group alone, while the apoptosis level in the combination group was significantly higher than that in the CXCL13 group alone (P=0.003). Conclusion CXCL13 promotes the radioresistance of lung cancer by activating Akt.

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Background: Malignant ventricular arrhythmia (MVA) can seriously affect the hemodynamic changes of the body. In this study, we developed and validated a nomogram to predict the in-hospital MVA risk in patients with STEMI after emergency PCI.Methods: The multivariable logistic regression analysis included variables with a P< 0.05 in the univariate logistic regression analysis and investigated the independent predictors affecting in-hospital MVA after PCI in patients with STEMI in the training cohort. The construction of a nomogram model used independent predictors to predict the risk of in-hospital MVA, and C-index, Hosmer-Lemeshow (HL) test, calibration curves, decision curve analysis (DCA), and receiver operating characteristic (ROC) were used to validate the nomogram.Results: Killip class [OR=5.034 (95% CI: 1.596– 15.809), P=0.005], CK-MB [OR=1.002 (95% CI: 1.001– 1.004), P=0.022], serum potassium [OR=0.618 (95% CI: 0.406– 0.918), P=0.020], NLR [OR=1.073 (95% CI: 1.034– 1.115), P< 0.001], and monocyte [OR=1.974 (95% CI: 1.376– 2.925), P< 0.001] were the independent predictors of in-hospital MVA after PCI in patients with STEMI. A nomogram including the 5 independent predictors was developed to predict the risk of in-hospital MVA. The C-index, equivalent to the area under the ROC curve (AUC), was 0.803 (95% confidence interval [CI]: 0.738– 0.868) in the training cohort, and 0.801 (95% CI:0.692– 0.911) in the validation cohort, showing that the nomogram had a good discrimination. The HL test (χ2=8.439, P=0.392 in the training cohort; χ2=9.730, P=0.285 in the validation cohort) revealed a good calibration. The DCA suggested an obvious clinical net benefit.Conclusion: Killip class, CK-MB, serum potassium, NLR, and monocyte were independent factors for in-hospital MVA after PCI in patients with STEMI. The nomogram model constructed based on the above factors to predict the risk of in-hospital MVA had satisfactory discrimination, calibration, and clinical effectiveness, and was an excellent tool for early prediction of the risk of in-hospital MVA after PCI in patients with STEMI. [ABSTRACT FROM AUTHOR]

The endoplasmic reticulum (ER) is the main storage site of Zn2+, and Zn2+ plays an important role in regulating ER homeostasis. Therefore, we designed and synthesized a ratiometric fluorescent Zn2+ probe ER-Zn targeting ER stress. The probe displayed a specific Zn2+ induced blue shift at the spectral maximum values of excitation (80 nm) and emission (30 nm). The ratio imaging capability of Zn2+ under dual excitation mode can be applied not only to quantitative and reversible detection of exogenous Zn2+, but also the observation of the Zn2+ level change under ER stress, elucidating the different behaviors of Zn2+ release in ER stimulated by tunicamycin and thapsigargin. Additionally, the NIR imaging capability of ER-Zn provides an important basis for further research on animal models and is expected to realize the visualization and treatment of ER stress-related diseases through the regulation of ER stress by Zn2+. We envision that this probe can be applied to screen drugs for diseases related to ER stress regulation. [ABSTRACT FROM AUTHOR]