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Objective: To observe the influence of different skin reactions of acupoint-application in summer on outcomes of prevention and treatment of bronchial asthma., Methods: All of the bronchial asthma patients were treated with acupoint-application therapy in the dog-days of summer, once every ten days, 3 times altogether. After the treatment, according to scores of the skin reactions including flush, itching, pain, vesicles, etc. induced by acupoint-application of prepared Chinese medical herb paste, 164 cases were divided into four groups: no reaction (n = 43), mild reaction (n = 42), moderate reaction (n = 39) and severe reaction (n = 40). One-year's follow-up was conducted after the treatment for assessing the asthma patients' cough-asthma score, wheezing rale score, and for recording the attack times., Results: After the treatment, the cough-asthma scores, wheezing rale scores, and asthmatic attack times were obviously decreased in all the 4 groups compared to pre-treatment (P < 0.05, P < 0.01). Comparison among the 4 groups showed that the levels of the 3 indexes were significantly higher in the no reaction group and mild reaction group than in the moderate and severe reaction groups after the treatment (P < 0.05, P < 0.01), suggesting a better therapeutic effect in patients undergoing moderate and severe skin reactions. There were no significant differences between the no reaction and mild reaction groups, and between the moderate reaction and severe reaction groups in the levels of the 3 indexes (P > 0. 05). The total effective rates of the no reaction, mild reaction, moderate reaction and severe reaction groups were 58.14%, 88.10%, 94.87% and 97.50%, respectively. The mild, moderate and severe reaction groups were better than the no reaction group in the therapeutic effect (P < 0.01). The mild reaction group was poorer than the moderate and severe reaction groups in clinical efficacy (P < 0.05). There was no marked difference between the moderate and severe reaction groups in clinical outcomes (P > 0.05)., Conclusion: Acupoint-application in the dog-days can effectively improve the asthma patients' symptoms, and the therapeutic effect of patients experiencing moderate and severe skin reactions was better.

Objective: To investigate the molecular characteristics of methicillin-resistant Staphylococcus aureus (MRSA) isolates from Chinese children in seven cities., Method: A total of 134 MRSA isolates were collected from nine hospitals. Multilocus sequence typing and spa typing were analyzed by polymerase chain reaction (PCR), and staphylococcal chromosomal cassette mec (SCCmec) type was analyzed by multiplex PCR. The Panton-Valentine leukocidin (pvl) gene was also detected., Result: Most MRSA strains were isolated from pneumonia and skin and soft tissue infection (SSTIs) patients, accounting for 82.1%. Overall, 16 sequence types (STs) were obtained, and CC59 (51.7%) was found to be the most prevalent, which included ST 59 and ST 338, followed by ST239 (16.4%). SCCmec types II, III, IV, and V were also identified in the current study. SCCmec type IV was the most predominant type at 50.0%, followed by SCCmec type V at 23.9% and III at 23.9%. SCCmec subtypes IVa, IVc, and IVg were found among SCCmec type IV strains, whereas IVa was the main subtype at 77.6%. Twenty-six spa types were also identified, among which the predominant type was t437 (47.8%). The prevalence of pvl genes and the SCCmec type of strain was relevant, and the pvl gene positive rate was higher in SCCmec type IV and V-type strains than in SCCmec type II and III strains (58.6% vs. 14.3%, P < 0.05); there was a significant difference between them. In the strains isolated from pneumonia and SSTIs, ST59-MRSA-IVa(t437) was the predominant clone. There were five clones detected from the strains isolated from septicemia, with ST59-MRSA-IVa(t437) and ST59-MRSA-V(t437) as the main clones (57.1%). Various predominant clones existed in different regions. ST59-MRSA-IVa(t437) was the prevalent clone in the Guangzhou, Beijing, Chongqing, and Shenzhen areas, whereas ST239-MRSA-III(t037) was the prevalent clone in the Shanghai area. Fifty percent of the isolates from the Wenzhou area belonged to ST910-MRSA-V(t318), whereas three clinical strains isolated from the Shenyang region belonged to three different types., Conclusion: The results indicate that MRSA isolates from Chinese children are largely associated with the ST59-MRSA-IV(t437) and ST239-MRSA-III(t037) clones. These two may belong to community-acquired MRSA and hospital-acquired ones, respectively. Different prevalent clones were detected in different diseases and different regions. Therefore, there is a need to conduct further research on clinical isolates, which can guide the choice of antibiotic treatment and the examination of MRSA prevalence.

Objective: To explore the influence of visual attention in visual evoked potential (VEP) examination., Methods: The pattern visual evoked potential (PVEP) and flash visual evoked potential (FVEP) were used to examine 110 normal subjects whose visual acuity or corrected visual acuity is in the range of 4.8-5.2, and age between 20 to 30 years old. In PVEP inspection, subjects were asked to watch with three ways, to stare at the central screen (at the same time count silently the number of alternating of black and white squares to improve the subjects' attention), to stare at the central screen at the same time thinking other things, to look sideways (the left eye stared at the left bottom corner of the screen or the right eye stared at the right one). In FVEP inspection, subjects were checked with eyes open and closed respectively. The latency and amplitude of P100 wave were recorded. The data were statistically analyzed by SPSS 13.0 software. RESULTS In PVEP inspection, the latency and amplitude of P100 wave were statistically different among three watching ways. In FVEP inspection, latency and amplitude of P100 wave were statistically different between eyes open and closed group., Conclusion: Although the visual evoked potential test is an objective electrophysiological visual function test, it is susceptible to the influence of various subjective factors such as visual attention. It should arise the attention of forensic experts.