1. Specific IgE and IgG measured by the MeDALL allergen-chip depend on allergen and route of exposure: The EGEA study
- Author
-
Isabelle Pin, M. Wickman, Mirela Curin, Christian Lupinek, Karin C. Lødrup Carlsen, Jean Bousquet, Rudolf Valenta, Yvonne Resch, Renata Kiss, I. Skrindo, Valérie Siroux, Jocelyne Just, Rachel Nadif, Susanne Vrtala, Thomas Keil, Josep M. Antó, Erik Melén, Institute for Advanced Biosciences / Institut pour l'Avancée des Biosciences (Grenoble) ( IAB ), Centre Hospitalier Universitaire [Grenoble] ( CHU ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Etablissement français du sang - Auvergne-Rhône-Alpes ( EFS ) -Centre National de la Recherche Scientifique ( CNRS ) -Université Grenoble Alpes ( UGA ), Service d'allergologie [CHU Trousseau], Assistance publique - Hôpitaux de Paris (AP-HP)-CHU Trousseau [APHP], Université Pierre et Marie Curie - Paris 6 - UFR de Médecine Pierre et Marie Curie ( UPMC ), Université Pierre et Marie Curie - Paris 6 ( UPMC ), Vieillissement et Maladies chroniques : approches épidémiologique et de santé publique ( VIMA ), Université de Versailles Saint-Quentin-en-Yvelines ( UVSQ ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ), Centre Hospitalier Régional Universitaire [Montpellier] ( CHRU Montpellier ), Institute for Advanced Biosciences / Institut pour l'Avancée des Biosciences (Grenoble) (IAB), Centre Hospitalier Universitaire [Grenoble] (CHU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Etablissement français du sang - Auvergne-Rhône-Alpes (EFS)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Department of Pathophysiology and Allergy Research [Vienna, Austria] (Division of Immunopathology), Medizinische Universität Wien = Medical University of Vienna, CHU Trousseau [APHP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Université Pierre et Marie Curie - Paris 6 - UFR de Médecine Pierre et Marie Curie (UPMC), Université Pierre et Marie Curie - Paris 6 (UPMC), Epidemiology and Health Economics [Berlin, Germany] (Institute of Social Medicine), Charité - UniversitätsMedizin = Charité - University Hospital [Berlin], Institute for Clinical Epidemiology and Biometry [Würzburg, Germany], Julius-Maximilians-Universität Würzburg (JMU), Department of Paediatrics [Oslo, Norway], Oslo University Hospital [Oslo], Institute of Clinical Medicine [Oslo], Faculty of Medicine [Oslo], University of Oslo (UiO)-University of Oslo (UiO), Institute of Environmental Medicine [Stockholm, Sweden], Karolinska Institutet [Stockholm]-Karolinska University Hospital [Stockholm]-Astrid Lindgren Children's Hospital, Sachs’ Children and Youth Hospital [Stockholm, Sweden], Vieillissement et Maladies chroniques : approches épidémiologique et de santé publique (VIMA), Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Institut National de la Santé et de la Recherche Médicale (INSERM), Department of Otorhinolaryngology [Lørenskog, Norway], Akershus University Hospital [Lørenskog], Christian Doppler Laboratory for the Development of Allergen chips [Vienna, Austria] (Department of Pathophysiology & Allergy Research), Medizinische Universität Wien = Medical University of Vienna-Center for Pathophysiology, Infectiology and Immunology [Vienna, Austria], Center for Research in Environmental Epidemiology (CREAL), Universitat Pompeu Fabra [Barcelona] (UPF)-Catalunya ministerio de salud, Hospital del Mar Medical Research Institute [Barcelona, Spain] (IMIM), Universitat Pompeu Fabra [Barcelona] (UPF), CIBER de Epidemiología y Salud Pública (CIBERESP), Mechanisms of the Development of Allergy (MeDALL-FP7), University of Groningen [Groningen]-Uppsala Universitet [Uppsala]-Universiteit Gent = Ghent University (UGENT)-Georg-August-University = Georg-August-Universität Göttingen, CHU Montpellier, Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier), The study was supported in part by Inserm Aviesan Itmo santé publique, the Scientific committee 'AGIR for chronic diseases', grant F4605 of the Austrian Science Fund (FWF) to RV and by the European Commission’s Seventh Framework 29 Program MeDALL under grant agreement no. 261357., European Project: 261357,EC:FP7:HEALTH,FP7-HEALTH-2010-single-stage,MEDALL(2010), Centre de l'Asthme et des Allergies [CHU Trousseau], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-CHU Trousseau [APHP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Sorbonne Université (SU), Julius-Maximilians-Universität Würzburg [Wurtzbourg, Allemagne] (JMU), Oslo University Hospital [Oslo, Norway], Georg-August-University [Göttingen]-University of Groningen [Groningen]-Uppsala Universitet [Uppsala]-Universiteit Gent = Ghent University [Belgium] (UGENT), Faraldo, Beatrice, Mechanisms of the Development of ALLergy - MEDALL - - EC:FP7:HEALTH2010-12-01 - 2015-05-31 - 261357 - VALID, Centre Hospitalier Universitaire [Grenoble] (CHU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Etablissement français du sang - Auvergne-Rhône-Alpes (EFS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), and Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université
- Subjects
0301 basic medicine ,Male ,Epidemiology ,[SDV]Life Sciences [q-bio] ,Microarray ,medicine.disease_cause ,Immunoglobulin E ,Airborne allergen ,Atopy ,Cohort Studies ,food allergens ,0302 clinical medicine ,Allergen ,immune system diseases ,MeDALL ,Immunology and Allergy ,[SDV.IMM.ALL]Life Sciences [q-bio]/Immunology/Allergology ,education.field_of_study ,biology ,Cohort ,Environmental exposure ,cohort ,Middle Aged ,respiratory system ,Allergen components ,3. Good health ,[SDV] Life Sciences [q-bio] ,EGEA ,Female ,epidemiology ,France ,IgE ,Respiratory allergens ,microarray ,Ragweed ,Adult ,Allergen immunotherapy ,IgG ,Immunology ,Population ,Cross Reactions ,03 medical and health sciences ,[ SDV.IMM.ALL ] Life Sciences [q-bio]/Immunology/Allergology ,medicine ,otorhinolaryngologic diseases ,Humans ,allergen components ,education ,Skin Tests ,Environmental Exposure ,Allergens ,medicine.disease ,biology.organism_classification ,Asthma ,respiratory tract diseases ,030104 developmental biology ,Cross-Sectional Studies ,030228 respiratory system ,[SDV.SPEE] Life Sciences [q-bio]/Santé publique et épidémiologie ,Immunoglobulin G ,biology.protein ,Food allergens ,Immunization ,[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie ,Follow-Up Studies ,respiratory allergens - Abstract
Background: The nature of allergens and route and dose of exposure may affect the natural development of IgE and IgG responses. Objective: We sought to investigate the natural IgE and IgG responses toward a large panel of respiratory and food allergens in subjects exposed to different respiratory allergen loads. Methods: A cross-sectional analysis was conducted in 340 adults of the EGEA (Epidemiological study of the Genetics and Environment of Asthma, bronchial hyperresponsiveness and atopy) (170 with and 170 without asthma) cohort. IgE and IgG responses to 47 inhalant and food allergen components were analyzed in sera using allergen microarray and compared between 5 French regions according to the route of allergen exposure (inhaled vs food allergens). Results: Overall 48.8% of the population had allergen-specific IgE levels of 0.3 ISAC standardized units (ISU) or more to at least 1 of the 47 allergens with no significant differences across the regions. For ubiquitous respiratory allergens (ie, grass, olive/ash pollen, house dust mites), specific IgE did not show marked differences between regions and specific IgG (≥0.5 ISU) was present in most subjects everywhere. For regionally occurring pollen allergens (ragweed, birch, cypress), IgE sensitization was significantly associated with regional pollen exposure. For airborne allergens cross-reacting with food allergens, frequent IgG recognition was observed even in regions with low allergen prevalence (Bet v 1) or for allergens less frequently recognized by IgE (profilins). Conclusions: The variability in allergen-specific IgE and IgG frequencies depends on exposure, route of exposure, and overall immunogenicity of the allergen. Allergen contact by the oral route might preferentially induce IgG responses. The study was supported in part by Inserm Aviesan Itmo santé publique, the Scientific committee “AGIR for chronic diseases,” grant F4605 of the Austrian Science Fund (FWF [Fonds zur Förderung der wissenschaftlichen Forschung]) to R.V. and by the European Commission's Seventh Framework 29 Program MeDALL under grant agreement no. 261357.
- Published
- 2017