Search

Your search keyword '"Gerber, Priscilla F."' showing total 332 results
Start Over Author "Gerber, Priscilla F."
332 results on '"Gerber, Priscilla F."'

2. Exploring the utility of bioaerosol metagenomics compared to PCRs for swine pathogen surveillance.

Author

Muwonge, Adrian, Gerber, Priscilla F., Wee, Bryan A., Thomson, Jill, Wang, Jingjing, Halbur, Patrick G., and Opriessnig, Tanja

Subjects
*SALIVA, *SWINE farms, *LIVESTOCK farms, *STREPTOCOCCUS suis, *SHOTGUN sequencing
Abstract

Introduction: Pathogen introduction and transmission at the farm, regional, or national level are associated with reduced animal welfare and negative impacts on herd economics. Ongoing infectious disease surveillance, active or passive, is therefore of high importance. For optimal resolution, each pig is sampled individually, for example by collecting blood or nasal swabs. In recent years, oral fluids have become very useful for population surveillance at the pen level. Another alternative is sampling the air to capture pathogens circulating across the entire barn via bioaerosols. Objective: This study aimed to examine the potential utility of bioaerosol metagenomics for pathogen detection on pig farms. Methods: Bioaerosols via automated air sampler, and oral fluid via pen-based ropes, were collected from each of two Scottish indoor pig farms. All samples were subjected to conventional routine bacterial isolation. Total genomic nucleic acids were extracted for PCR screening for three pig DNA viruses, three bacterial Mycoplasma species and an RNA virus. Illumina shotgun metagenomic sequencing was also conducted. Results: Oral fluids contained more DNA compared to bioaerosol samples. DNA integrity exhibited limited impact on PCR or sequence yield. While Streptococcus suis could be cultured from a single oral fluid sample, reads mapped to S. suis were detectable in all metagenomic samples. Other bacterial pig pathogens, including Mycoplasma hyorhinis, M. hyopneumoniae and M. hyosynoviae, were detected in oral fluid and aerosols by PCR and metagenomics. One of the two farms was PRRSV positive, and the virus was detectable via PCR in oral fluids but not in bioaerosols. Antimicrobial resistance (AMR) gene profiles had less variation between bioaerosols and oral fluids. Some identified AMR genes had strikingly similar abundance overall. Conclusion: Overall, these findings indicate that there is potential utility of bioaerosol metagenomics for pathogen surveillance on pig farms; however, more research is needed for technical and cost optimization to allow for routine pathogen detection on livestock farms. [ABSTRACT FROM AUTHOR]

Published
2024
Full Text
View/download PDF

13. Failure to experimentally infect 10 days-old piglets with a cell culture-propagated infectious stock of a classical genotype 1a porcine epidemic diarrhea virus

Author

Gerber, Priscilla F, Cao, Dianjun, Xiao, Chao-Ting, Chen, Qi, Lager, Kelly, Bosch, Berend Jan, Meng, Xiang-Jin, Halbur, Patrick G, Opriessnig, Tanja, Gerber, Priscilla F, Cao, Dianjun, Xiao, Chao-Ting, Chen, Qi, Lager, Kelly, Bosch, Berend Jan, Meng, Xiang-Jin, Halbur, Patrick G, and Opriessnig, Tanja

Abstract

INTRODUCTION: Porcine epidemic diarrhea virus (PEDV) causes enteric disease in pigs of all ages. PEDV can be grouped into G1 (classical strains) and G2 (variant strains) based on sequence differences in the spike gene. Although several pathogenesis studies using contemporary strains of PEDV have been conducted to date, there is limited information on the pathogenesis of historical PEDV strains in contemporary pigs. This study aimed to investigate the clinical disease course of 10 days-old pigs infected with a classical European G1a PEDV strain from the 1980s which was last passaged in pigs in 1994.METHODS: Sequencing results confirmed that the virus inoculum was a PEDV strain closely related to the prototype CV777 strain. The PEDV stock was serially passaged three times in Vero cells, and the P3 infectious virus stock was used to inoculate the pigs. A total of 40 pigs were inoculated using the oral route.RESULTS: Pigs showed no enteric disease signs, and PEDV shedding was not detected for 44 days post-inoculation (dpi). At necropsy at 3 (5 pigs) or 7 dpi (5 pigs), no lesions were observed in intestinal sections, which were negative for PEDV antigen by immunohistochemistry. In addition, no IgG or IgA PEDV-specific antibodies in serum or fecal samples for 35 dpi further indicates a lack of infection. Titration of the leftover thawed and refrozen PEDV virus stock inoculum showed that the virus stock retained its infectivity in Vero cell culture and the porcine small intestine enterocytes cell line IPEC-J2.DISCUSSION: The reasons for the loss of infectivity in pigs are unknown. In conclusion, we showed that a classical G1a PEDV strain successfully propagated in cell cultures could not orally infect 40 piglets.

Published
2023

14. Failure to experimentally infect 10 days-old piglets with a cell culture-propagated infectious stock of a classical genotype 1a porcine epidemic diarrhea virus

Author

Virologie, Infectious Diseases and Immunology - Virology, Gerber, Priscilla F, Cao, Dianjun, Xiao, Chao-Ting, Chen, Qi, Lager, Kelly, Bosch, Berend Jan, Meng, Xiang-Jin, Halbur, Patrick G, Opriessnig, Tanja, Virologie, Infectious Diseases and Immunology - Virology, Gerber, Priscilla F, Cao, Dianjun, Xiao, Chao-Ting, Chen, Qi, Lager, Kelly, Bosch, Berend Jan, Meng, Xiang-Jin, Halbur, Patrick G, and Opriessnig, Tanja

Published
2023

41. Additional file 7 of Microbial taxa in dust and excreta associated with the productive performance of commercial meat chicken flocks

Author

Bindari, Yugal Raj, Moore, Robert J., Van, Thi Thu Hao, Walkden-Brown, Stephen W., and Gerber, Priscilla F.

Abstract

Additional file 7. Distinguishing taxa between dust and excreta stratified by company. Linear discriminant analysis effect size was performed on the top 50 most abundant bacterial taxa (genus level) across all ages.

Published
2021
Full Text
View/download PDF

42. Additional file 10 of Microbial taxa in dust and excreta associated with the productive performance of commercial meat chicken flocks

Author

Bindari, Yugal Raj, Moore, Robert J., Van, Thi Thu Hao, Walkden-Brown, Stephen W., and Gerber, Priscilla F.

Abstract

Additional file 10. Distinguishing taxa between companies (A and B) stratified by sample type (dust and excreta). Linear discriminant analysis effect size was performed on the top 50 most abundant bacterial taxa (genus level) across all ages.

Published
2021
Full Text
View/download PDF

43. Additional file 2 of Microbial taxa in dust and excreta associated with the productive performance of commercial meat chicken flocks

Author

Bindari, Yugal Raj, Moore, Robert J., Van, Thi Thu Hao, Walkden-Brown, Stephen W., and Gerber, Priscilla F.

Abstract

Additional file 2. Permutational multivariate analysis of variance (PERMANOVA) results showing the influence of sample type, company, age, performance and their interaction on the bacterial community structure. Df = degrees of freedom, R² = proportion of the variance explained by each model, PERMDISP = permutational analyses of multivariate dispersion.

Published
2021
Full Text
View/download PDF

44. Additional file 4 of Microbial taxa in dust and excreta associated with the productive performance of commercial meat chicken flocks

Author

Bindari, Yugal Raj, Moore, Robert J., Van, Thi Thu Hao, Walkden-Brown, Stephen W., and Gerber, Priscilla F.

Subjects
animal structures, digestive, oral, and skin physiology, complex mixtures
Abstract

Additional file 4. Taxonomic assignment of the bacteria at phylum level in dust and excreta stratified by company.

Published
2021
Full Text
View/download PDF

50. Detection and Quantification of Clostridium perfringens and Eimeria spp. in Poultry Dust Using Real-Time PCR Under Experimental and Field Conditions

Author

Ahaduzzaman, Md, primary, Keerqin, Chake, additional, Kumar, Alip, additional, Musigwa, Sosthene, additional, Morgan, Natalie, additional, Kheravii, Sarbast K., additional, Sharpe, Sue, additional, Williamson, Sarah, additional, Wu, Shu-Biao, additional, Walkden-Brown, Stephen W., additional, and Gerber, Priscilla F., additional

Published
2020
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results