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Abstract Adoption of a health information system is always a challenge for hospitals. It is because most of the medical staff do not have enough skills to use the new technology and due to the sensitivity of medical data. These factors pose a challenge for the successful adoption of health information system in hospitals. The aim of this research is to find out the factors which influence the adoption of information systems in hospitals. The study investigated the impact of the Financial status of the Hospital; Perceived Usefulness and Perceived Ease of Use on the adoption of health information systems through a questionnaire survey. Data was collected from 602 healthcare workers from 20 hospitals through close-ended questionnaire in Pakistan, where the adoption of health information systems is very slow. PLS-SEM was used for the analysis. The findings show that the Financial status of the Hospital; Perceived Usefulness and Perceived Ease of Use have positive and significant role in the adoption of Health Information Systems. The finding also shows that hospital size moderates the relationship of Perceived ease of use and the adoption of health information systems and interestingly it does not moderate the relationship among perceived usefulness and financial strength toward the adoption of health information systems. The study concludes that perceived ease of use, perceived usefulness and financial strength are the main factors, necessary for the adoption of health information systems. The findings of the study have useful implications for policy makers, medical professionals to successfully adopt health information systems in hospitals. It also provides new avenues for researchers to explore other factors and test this framework in other countries.