Search

Your search keyword '"Ghaedi, Maryam"' showing total 34 results
Start Over Author "Ghaedi, Maryam"
34 results on '"Ghaedi, Maryam"'

4. Characterization of innate lymphoid cell subsets infiltrating melanoma and epithelial ovarian tumors.

Author

Chung, Douglas C., Ghaedi, Maryam, Warner, Kathrin, Sayad, Azin, Saibil, Samuel D., Bernardini, Marcus Q., Clarke, Blaise A., Shaw, Patricia A., Butler, Marcus O., Easson, Alexandra, Morrissy, Sorana, Wang, Ben X., Nguyen, Linh, Ohashi, Pamela S., and Jacquelot, Nicolas

Subjects
*INNATE lymphoid cells, *MYELOID cells, *EPITHELIAL tumors, *OVARIAN epithelial cancer, *OVARIAN tumors
Abstract

The innate lymphoid cell (ILC) family is composed of heterogeneous innate effector and helper immune cells that preferentially reside in tissues where they promote tissue homeostasis. In cancer, they have been implicated in driving both pro- and anti-tumor responses. This apparent dichotomy highlights the need to better understand differences in the ILC composition and phenotype within different tumor types that could drive seemingly opposite anti-tumor responses. Here, we characterized the frequency and phenotype of various ILC subsets in melanoma metastases and primary epithelial ovarian tumors. We observed high PD-1 expression on ILC subsets isolated from epithelial ovarian tumor samples, while ILC populations in melanoma samples express higher levels of LAG-3. In addition, we found that the frequency of cytotoxic ILCs and NKp46+ILC3 in tumors positively correlates with monocytic cells and conventional type 2 dendritic cells, revealing potentially new interconnected immune cell subsets in the tumor microenvironment. Consequently, these observations may have direct relevance to tumor microenvironment composition and how ILC subset may influence anti-tumor immunity. [ABSTRACT FROM AUTHOR]

Published
2024
Full Text
View/download PDF

7. Human CD127 negative ILC2s show immunological memory

Author

Mathä, Laura, Krabbendam, Lisette, Martinez Høyer, Sergio, Heesters, Balthasar A, Golebski, Korneliusz, Kradolfer, Chantal, Ghaedi, Maryam, Ma, Junjie, Stadhouders, Ralph, Bachert, Claus, Cardell, Lars-Olaf, Zhang, Nan, Holtappels, Gabriele, Reitsma, Sietze, Helgers, Leanne Carijn, Geijtenbeek, Teunis B H, Coquet, Jonathan M, Takei, Fumio, Spits, Hergen, Martinez-Gonzalez, Itziar, Mathä, Laura, Krabbendam, Lisette, Martinez Høyer, Sergio, Heesters, Balthasar A, Golebski, Korneliusz, Kradolfer, Chantal, Ghaedi, Maryam, Ma, Junjie, Stadhouders, Ralph, Bachert, Claus, Cardell, Lars-Olaf, Zhang, Nan, Holtappels, Gabriele, Reitsma, Sietze, Helgers, Leanne Carijn, Geijtenbeek, Teunis B H, Coquet, Jonathan M, Takei, Fumio, Spits, Hergen, and Martinez-Gonzalez, Itziar

Abstract

ILC2s are key players in type 2 immunity and contribute to maintaining homeostasis. ILC2s are also implicated in the development of type 2 inflammation-mediated chronic disorders like asthma. While memory ILC2s have been identified in mouse, it is unknown whether human ILC2s can acquire immunological memory. Here, we demonstrate the persistence of CD45RO, a marker previously linked to inflammatory ILC2s, in resting ILC2s that have undergone prior activation. A high proportion of these cells concurrently reduce the expression of the canonical ILC marker CD127 in a tissue-specific manner. Upon isolation and in vitro stimulation of CD127-CD45RO+ ILC2s, we observed an augmented ability to proliferate and produce cytokines. CD127-CD45RO+ ILC2s are found in both healthy and inflamed tissues and display a gene signature of cell activation. Similarly, mouse memory ILC2s show reduced expression of CD127. Our findings suggest that human ILC2s can acquire innate immune memory and warrant a revision of the current strategies to identify human ILC2s.

Published
2024

10. A novel population of CD103-expressing CD56 +regulatory ILCs suppresses intratumoural T cells and are associated with poor prognosis in patients with ovarian carcinoma

Author

Chung, Douglas, primary, Vakharia, Jehan, additional, Warner, Kathrin, additional, Jacquelot, Nicolas, additional, Sayad, Azin, additional, Han, SeongJun, additional, Ghaedi, Maryam, additional, Garcia-Batres, Carlos, additional, Elford, Alisha R, additional, Matthews, Jessica A, additional, Wang, Ben X, additional, Nguyen, Linh T, additional, Shaw, Patricia A, additional, Clarke, Blaise A, additional, Bernardini, Marcus Q, additional, Ferguson, Sarah E, additional, Crome, Sarah Q, additional, and Ohashi, Pamela S, additional

Published
2023
Full Text
View/download PDF

28. Evaluation of Bacterial Contamination of Water Supply in Dental Unit Water Lines at Zahedan Dental School 2008

Author

Mollashahi Leila, Shahraki Shahram, Honarmand Marieh, Gholipur Roghayeh, and Ghaedi Maryam

Subjects
Biofilm, lcsh:R, lcsh:Medicine, Cfu/ml, Dental unit water
Abstract

Background: Assessment of microbial contamination in dental unit waterlines has been focused on because of high risk of dangerous infections in immunocompromised patients. The purpose of this study was to evaluate the bacterial contamination of water supply in dental unit water lines at Zahedan Dental School.Materials and Methods: In this descriptive analytical study we investigated 400 water samples collected from four parts of each unit including air/water syringe, turbine handpiece (before & after flushing), cup filler and 1 water sample collected from city water reservoir in Zahedan faculty of dentistry during 2008. Water samples were taken on Saturdays (the first working day in a week) and Wednesdays (the last working day in a week), before and after treatment on the same unit. Samples were transported in closed sterile containers to microbiology laboratory. All samples were incubated on blood agar and McCankey plates for 72 hours at 37°C. Bacterial contamination were then evaluted. Data were analyzed by ANOVA and t-test.Results: Total mean bacterial count was 6914 cfu/ml. Mean bacterial contamination on Saturdays (8859 cfu/ml) were higher than Wednesdays (4969 cfu/ml). Mean bacterial contamination before treatment was (5155 cfu/ml) less than the end of treatment (8673 cfu/ml) on the same unit. Mean bacterial contaminations of prosthetics clinic (13439cfu/ml) was higher than other clinics. The mean of periodentology clinic bacterial contaminations (3012 cfu/ml) was the least.Conclusion: The result of this study demonstrated that microbiological level of dental unit water lines is high. The dentists must be aware of the high level of microorganisms in the dental unit's water and thus minimize the risk of infection in both staff and patients.

Published
2010

33. Single-cell analysis of RORα tracer mouse lung reveals ILC progenitors and effector ILC2 subsets

Author

Ghaedi, Maryam, Shen, Zi Yi, Orangi, Mona, Martinez-Gonzalez, Itziar, Wei, Lisa, Lu, Xiaoxiao, Das, Arundhoti, Heravi-Moussavi, Alireza, Marra, Marco A., Bhandoola, Avinash, and Takei, Fumio

Abstract

Lung group 2 innate lymphoid cells (ILC2s) drive allergic inflammation and promote tissue repair. ILC2 development is dependent on the transcription factor retinoic acid receptor–related orphan receptor (RORα), which is also expressed in common ILC progenitors. To elucidate the developmental pathways of lung ILC2s, we generated RORα lineage tracer mice and performed single-cell RNA sequencing, flow cytometry, and functional analyses. In adult mouse lungs, we found an IL-18Rα+ST2− population different from conventional IL-18Rα−ST2+ ILC2s. The former was GATA-3intTcf7EGFP+Kit+, produced few cytokines, and differentiated into multiple ILC lineages in vivo and in vitro. In neonatal mouse lungs, three ILC populations were identified, namely an ILC progenitor population similar to that in adult lungs and two distinct effector ILC2 subsets that differentially produced type 2 cytokines and amphiregulin. Lung ILC progenitors might actively contribute to ILC-poiesis in neonatal and inflamed adult lungs. In addition, neonatal lung ILC2s include distinct proinflammatory and tissue-repairing subsets.

Published
2020
Full Text
View/download PDF

34. Human CD127 negative ILC2s show immunological memory.

Author

Mathä L, Krabbendam L, Martinez Høyer S, Heesters BA, Golebski K, Kradolfer C, Ghaedi M, Ma J, Stadhouders R, Bachert C, Cardell LO, Zhang N, Holtappels G, Reitsma S, Helgers LC, Geijtenbeek TBH, Coquet JM, Takei F, Spits H, and Martinez-Gonzalez I

Subjects
Humans, Animals, Mice, Leukocyte Common Antigens metabolism, Cytokines metabolism, Inflammation immunology, Female, Mice, Inbred C57BL, Immunologic Memory immunology, Interleukin-7 Receptor alpha Subunit metabolism, Lymphocytes immunology, Immunity, Innate immunology
Abstract

ILC2s are key players in type 2 immunity and contribute to maintaining homeostasis. ILC2s are also implicated in the development of type 2 inflammation-mediated chronic disorders like asthma. While memory ILC2s have been identified in mouse, it is unknown whether human ILC2s can acquire immunological memory. Here, we demonstrate the persistence of CD45RO, a marker previously linked to inflammatory ILC2s, in resting ILC2s that have undergone prior activation. A high proportion of these cells concurrently reduce the expression of the canonical ILC marker CD127 in a tissue-specific manner. Upon isolation and in vitro stimulation of CD127-CD45RO+ ILC2s, we observed an augmented ability to proliferate and produce cytokines. CD127-CD45RO+ ILC2s are found in both healthy and inflamed tissues and display a gene signature of cell activation. Similarly, mouse memory ILC2s show reduced expression of CD127. Our findings suggest that human ILC2s can acquire innate immune memory and warrant a revision of the current strategies to identify human ILC2s., (© 2024 Mathä et al.)

Published
2024
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results