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Background: Tissue staining is pivotal in histology and histopathology, shouldering a noteworthy role in identifying and classifying tissues and diseases. Due to their non-production of toxic effluents, the utilization of plant-based dyes aligns harmoniously with environmental sustainability and the well-being of laboratory personnel and the general public. Furthermore, this approach is highly cost-effective, further enhancing its appeal. Objectives: This research study explored the feasibility of staining various tissues in mice, such as the liver, kidney, intestine, and cartilage, utilizing a dye extracted from black carrots. Methods: An ethanol extract of 200 g of fresh black carrots (Daucus carota L.) was prepared using 95% ethanol saturated with two different solvents in 200 mL of distilled water. Subsequently, the prepared sections of mice tissue were immersed in the extracted dye solution for 20 minutes, followed by assessment using a light microscope. Hematoxylin-eosin staining was used as a control. Results: The dye extracted from the black carrot using alum and acetic acid successfully stained the cartilage, kidney, intestine, and liver tissues, giving them a bluish-gray coloration. Phytochemical screening further confirmed the presence of anthocyanins in the black carrot extract. Conclusion: The dye derived from black carrots exhibits natural tissue staining capabilities, making it an alternative to hematoxylin-eosin in histology and histopathology laboratories. [ABSTRACT FROM AUTHOR]

The purpose of this research was to look for primordial germ cells in male gonads at various stages of the pheasant embryos. Primordial germ cells are cells that differentiate into sperm or oocyte and are important for the transmission of genetic information across generations. In this study, embryos were extracted in embryonic days 8-24. The primordial germ cells were recognized in paraffin and resin tissue sections on days 8 to 10 of embryogenesis owing to the specific properties of these cells, which included a large size, large nucleus and nucleolus, and little cytoplasm. The primordial germ cells in the testes were identified using histochemical and immunostaining techniques. Periodic Acid-Schiff method, immunohistochemistry using stage-specific embryonic antigen-1, alkaline phosphatase and toluidine blue staining were used to identify primordial germ cells. Germ cells were detected only in semithin sections at older ages (10-24 days). Moreover, the response of these cells was negative, at all ages, to alkaline phosphatase, Periodic Acid-Schiff staining and stage-specific embryonic antigen-1 reaction. The findings of this study revealed that primordial germ cells in the testicular tissue of pheasant embryos of all ages tested responded adversely to histochemical and immunohistochemical techniques. Also, this study showed that routine staining of hematoxylin and eosin and using semithin sections are suitable for the histological diagnosis of these cells. [ABSTRACT FROM AUTHOR]