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Objectives To assess livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) carriage among workers in pig slaughterhouses and assess associated risk factors, including occupational exposure to LA-MRSA. Methods A cross-sectional study in three Dutch pig slaughterhouses was undertaken. Nasal swabs of participants were taken. Nasal swabs and surface wipes, air and glove samples were screened for presence of methicillin-resistant Staphylococcus aureus (MRSA). MRSA was quantitatively determined on gloves and in air samples by culturing and real-time PCR. Results 11 of 341 (3.2%) participants were identified as nasal MRSA carriers. MRSA-positive workers were predominantly found at the start of the slaughter process. Major risk factors for carriage were working in the lairage and working in the scalding and dehairing area. Most nasal isolates (73%) belonged to the LA-MRSA clone ST398. MRSA ST398-positive environmental samples were found throughout the slaughter process. A clear decrease was seen along the slaughterline in the number of MRSA-positive samples and in the MRSA amount per sample. Conclusions This study showed that working in the lairage area or scalding and dehairing area were the major risk factors for MRSA carriage in pig slaughterhouse workers, while the overall prevalence of MRSA carriage is low. Occupational exposure to MRSA decreased along the slaughterline, and the risk of carriage showed a parallel decrease. [ABSTRACT FROM AUTHOR]

Strain superlattices (SL) in 2D materials like graphene provide an ideal test bed for generating flat bands and exploring the effects of strong correlations. Here we report STM/STS measurements on an engineered SL generated by placing graphene on a periodic array of silica nanospheres. A pseudomagnetic field as high as 55 T is observed along with the formation of pseudo-Landau levels (pLLs), not only at the expected integer values but also at fractional values. In regions where the Fermi energy intersects the zeroth pLL, we observe that this pLL splits. Using tight binding calculations, we show that our system supports formation of quasi-flat bands. We are also able to simulate the strain induced pLL splitting and show how on-site interaction may create fractional pLLs. Thus, we have demonstrated a customizable, reproducible, and scalable graphene strain superlattice system that can host a range of different correlation driven states.

Superconducting diode effects have recently attracted much attention for their potential applications in superconducting logic circuits. Several pathways have been proposed to give rise to non-reciprocal critical currents in various superconductors and Josephson junctions. In this work, we establish the presence of a large Josephson diode effect in a type-II Dirac semimetal 1T-PtTe 2 facilitated by its helical spin-momentum locking and distinguish it from extrinsic geometric effects. The magnitude of the Josephson diode effect is shown to be directly correlated to the large second-harmonic component of the supercurrent. We denote such junctions, where the relative phase between the two harmonics can be tuned by a magnetic field, as 'tunable second order φ 0 -junctions'. The direct correspondence between the second harmonic supercurrents and the diode effect in 1T-PtTe 2 junctions at relatively low magnetic fields makes it an ideal platform to study the Josephson diode effect and Cooper quartet transport in Josephson junctions., Competing Interests: Competing interestsThe authors declare no competing interests., (© The Author(s) 2024.)

Background: Attractant-based trapping is used in the establishment of pest-free areas and areas of low pest prevalence for fruit flies (Diptera: Tephritidae). Male lures are commonly used attractants in fruit fly trapping. In this study, the effects of male lure dispensers, traps and combinations of dispensers and traps on monitoring of two fruit fly pests, Ceratitis capitata (Wiedemann) and Bactrocera dorsalis (Hendel), were investigated in South Africa., Results: In the male-lure-based trapping systems evaluated, trimedlure (TML) for C. capitata and methyl eugenol (ME) for B. dorsalis, the type of dispenser affected catches for both species. Higher catches of B. dorsalis males were recorded in bucket traps baited with a dispenser containing 15 g ME compared with traps baited with dispensers containing either 4 g ME or 2 g ME. Catches of C. capitata males were higher with dispensers containing TML than those with TML plus extender (Capilure®). The type of trap used with TML also influenced catches of C. capitata with higher numbers recorded in yellow Delta trap compared with the Sensus bucket trap., Conclusions: Dispensers with higher ME loadings are more effective for monitoring of B. dorsalis. The yellow Delta trap baited with TML (without extender) would be an effective monitoring system for C. capitata. Fruit fly prevalence levels as determined by specific trapping systems should be related to their efficiency in terms of catches of the target pests. © 2020 Society of Chemical Industry., (© 2020 Society of Chemical Industry.)

Artificially engineered topological superconductivity has emerged as a viable route to create Majorana modes. In this context, proximity-induced superconductivity in materials with a sizable spin-orbit coupling has been intensively investigated in recent years. Although there is convincing evidence that superconductivity may indeed be induced, it has been difficult to elucidate its topological nature. Here, we engineer an artificial topological superconductor by progressively introducing superconductivity (Nb), strong spin-orbital coupling (Pt), and topological states (Bi 2 Te 3 ). Through spectroscopic imaging of superconducting vortices within the bare s -wave superconducting Nb and within proximitized Pt and Bi 2 Te 3 layers, we detect the emergence of a zero-bias peak that is directly linked to the presence of topological surface states. Our results are rationalized in terms of competing energy trends which are found to impose an upper limit to the size of the minigap separating Majorana and trivial modes, its size being ultimately linked to fundamental materials properties.

Background : Over the next 25 years, the global prevalence of diabetes is expected to grow to affect 700 million individuals. Consequently, an unprecedented number of patients will be at risk for vision loss from diabetic eye disease. This demand will almost certainly exceed the supply of eye care professionals to individually evaluate each patient on an annual basis, signaling the need for 21st century tools to assist our profession in meeting this challenge. Methods: Review of available literature on artificial intelligence (AI) as applied to diabetic retinopathy (DR) detection and prediction Results : The field of AI has seen exponential growth in evaluating fundus photographs for DR. AI systems employ machine learning and artificial neural networks to teach themselves how to grade DR from libraries of tens of thousands of images and may be able to predict future DR progression based on baseline fundus photographs. Conclusions : AI algorithms are highly promising for the purposes of DR detection and will likely be able to reliably predict DR worsening in the future. A deeper understanding of these systems and how they interpret images is critical as they transition from the bench into the clinic.

Recent population growth of the harbour porpoise ( Phocoena phocoena ), grey seal ( Halichoerus grypus ) and common seal ( Phoca vitulina ) in the North Sea has increased potential interaction between these species. Grey seals are known to attack harbour porpoises. Some harbour porpoises survive initially, but succumb eventually, often showing severely infected skin lesions. Bacteria transferred from the grey seal oral cavity may be involved in these infections and eventual death of the animal. In humans, seal bites are known to cause severe infections. In this study, a 16S rRNA-based microbiome sequencing approach is used to identify the oral bacterial diversity in harbour porpoises, grey seals and common seals; detect the potential transfer of bacteria from grey seals to harbour porpoises by biting and provide insights in the bacteria with zoonotic potential present in the seal oral cavity. β-diversity analysis showed that 12.9% (4/31) of the harbour porpoise skin lesion microbiomes resembled seal oral microbiomes, while most of the other skin lesion microbiomes also showed seal-associated bacterial species, including potential pathogens. In conclusion, this study shows that bacterial transmission from grey seals to harbour porpoises by biting is highly likely and that seal oral cavities harbour many bacterial pathogens with zoonotic potential., Competing Interests: We have no competing interests., (© 2020 The Authors.)

Neisseria animaloris is considered to be a commensal of the canine and feline oral cavities. It is able to cause systemic infections in animals as well as humans, usually after a biting trauma has occurred. We recovered N. animaloris from chronically inflamed bite wounds on pectoral fins and tailstocks, from lungs and other internal organs of eight harbour porpoises. Gross and histopathological evidence suggest that fatal disseminated N. animaloris infections had occurred due to traumatic injury from grey seals. We therefore conclude that these porpoises survived a grey seal predatory attack, with the bite lesions representing the subsequent portal of entry for bacteria to infect the animals causing abscesses in multiple tissues, and eventually death. We demonstrate that forensic microbiology provides a useful tool for linking a perpetrator to its victim. Moreover, N. animaloris should be added to the list of potential zoonotic bacteria following interactions with seals, as the finding of systemic transfer to the lungs and other tissues of the harbour porpoises may suggest a potential to do likewise in humans.

Species of the Epsilonproteobacteria genera Arcobacter, Campylobacter , and Helicobacter are commonly associated with vertebrate hosts and some are considered significant pathogens. Vertebrate-associated Epsilonproteobacteria are often considered to be largely confined to endothermic mammals and birds. Recent studies have shown that ectothermic reptiles display a distinct and largely unique Epsilonproteobacteria community, including taxa which can cause disease in humans. Several Arcobacter taxa are widespread amongst reptiles and often show a broad host range. Reptiles carry a large diversity of unique and novel Helicobacter taxa, which apparently evolved in an ectothermic host. Some species, such as Campylobacter fetus , display a distinct intraspecies host dichotomy, with genetically divergent lineages occurring either in mammals or reptiles. These taxa can provide valuable insights in host adaptation and co-evolution between symbiont and host. Here, we present an overview of the biodiversity, ecology, epidemiology, and evolution of reptile-associated Epsilonproteobacteria from a broader vertebrate host perspective.

As experimental probes have matured to observe ultrafast transient and high frequency responses of materials and devices, so to have the theoretical methods to numerically and analytically simulate time- and frequency-resolved transport. In this review article, we discuss recent progress in the development of the time-dependent and frequency-dependent non-equilibrium Green function (NEGF) technique. We begin with an overview of the theoretical underpinnings of the underlying Kadanoff-Baym equations and derive the fundamental NEGF equations in the time and frequency domains. We discuss how these methods have been applied to a variety of condensed matter systems such as molecular electronics, nanoscale transistors, and superconductors. In addition, we survey the application of NEGF in fields beyond condensed matter, where it has been used to study thermalization in ultra-cold atoms and to understand leptogenesis in the early universe. Throughout, we pay special attention to the challenges of incorporating contacts and interactions, as the NEGF method is uniquely capable of accounting for such features.

Unconventional superconductivity arising from the interplay between strong spin-orbit coupling and magnetism is an intensive area of research. One form of unconventional superconductivity arises when Cooper pairs subjected to a magnetic exchange coupling acquire a finite momentum. Here, we report on a signature of finite momentum Cooper pairing in the three-dimensional topological insulator Bi 2 Se 3 . We apply in-plane and out-of-plane magnetic fields to proximity-coupled Bi 2 Se 3 and find that the in-plane field creates a spatially oscillating superconducting order parameter in the junction as evidenced by the emergence of an anomalous Fraunhofer pattern. We describe how the anomalous Fraunhofer patterns evolve for different device parameters, and we use this to understand the microscopic origin of the oscillating order parameter. The agreement between the experimental data and simulations shows that the finite momentum pairing originates from the coexistence of the Zeeman effect and Aharonov-Bohm flux.

During a study to assess the faecal microbiome of common seals (Phoca vitulina) in a Dutch seal rehabilitation centre, 16S rRNA gene sequences of an unknown Campylobacter taxon were identified. Campylobacter isolates, which differed from the established Campylobacter taxa, were cultured and their taxonomic position was determined by a polyphasic study based on ten isolates. The isolates were characterized by 16S rRNA and atpA gene sequence analyses and by conventional phenotypic testing. Based on the whole genome sequences, the average nucleotide identity and core genome phylogeny were determined. The isolates formed a separate phylogenetic clade, divergent from all other Campylobacter taxa and most closely related to Campylobacter corcagiensis, Campylobacter geochelonis and Campylobacter ureolyticus. The isolates can be distinguished phenotypically from all other Campylobacter taxa based on their lack of motility, growth at 25 °C and growth on MacConkey agar. This study shows that these isolates represent a novel species within the genus Campylobacter, for which the name Campylobacter blaseri sp. nov. is proposed. The type strain for this novel species is 17S00004-5 T (=LMG 30333 T =CCUG 71276 T ).

Scirtothrips aurantii Faure (Thysanoptera: Thripidae) is a major pest of citrus fruit in subtropical southern Africa. Population monitoring is an important aspect of S. aurantii control, but additional information is required on its phenology. Dispersal of mature larvae onto the soil surface from the tree canopy, and emergence of adults, were assessed using dispersal/emergence (D/E) traps in an untreated citrus orchard in South Africa. Overall, 90.7% of adult Thysanoptera emerging from soil / leaf litter beneath the citrus trees were S. aurantii, of which 35.7% were males, and 64.3% were females. Female S. aurantii having survived winter as adults oviposited on the early spring flush. This resulted in the first population peak of larvae dropping to the ground to pupate and adults emerging in spring, September to early October, as the first vegetative flush of the citrus-growing season hardened, and fruit was set as blossoming ended. Initial infestation of young fruit occurred after a build-up of larval numbers on the soft citrus flush late July to early August. The second generation larval and adult peak occurred late November to early December, whether there was new flush or not, as young fruit could support the S. aurantii population. A third peak occurred mid-December to late January, depending on year. Thus, there were three generations of S. aurantii during the period of citrus fruit susceptibility to thrips damage (September-January). After the autumn flush in April, another peak of larvae and adults occurred before the population declined to a minimum from May to July.

Homologous recombination is a major driver of bacterial speciation. Genetic divergence and host association are important factors influencing homologous recombination. Here, we study these factors for Campylobacter fetus, which shows a distinct intraspecific host dichotomy. Campylobacter fetus subspecies fetus (Cff) and venerealis are associated with mammals, whereas C. fetus subsp. testudinum (Cft) is associated with reptiles. Recombination between these genetically divergent C. fetus lineages is extremely rare. Previously it was impossible to show whether this barrier to recombination was determined by the differential host preferences, by the genetic divergence between both lineages or by other factors influencing recombination, such as restriction-modification, CRISPR/Cas, and transformation systems. Fortuitously, a distinct C. fetus lineage (ST69) was found, which was highly related to mammal-associated C. fetus, yet isolated from a chelonian. The whole genome sequences of two C. fetus ST69 isolates were compared with those of mammal- and reptile-associated C. fetus strains for phylogenetic and recombination analysis. In total, 5.1-5.5% of the core genome of both ST69 isolates showed signs of recombination. Of the predicted recombination regions, 80.4% were most closely related to Cft, 14.3% to Cff, and 5.6% to C. iguaniorum. Recombination from C. fetus ST69 to Cft was also detected, but to a lesser extent and only in chelonian-associated Cft strains. This study shows that despite substantial genetic divergence no absolute barrier to homologous recombination exists between two distinct C. fetus lineages when occurring in the same host type, which provides valuable insights in bacterial speciation and evolution.

Reptiles have been shown to host a significant Helicobacter diversity. In order to survive, reptile-associated Helicobacter lineages need to be adapted to the thermally dynamic environment encountered in a poikilothermic host. The whole genomes of reptile-associated Helicobacter lineages can provide insights in Helicobacter host adaptation and coevolution. These aspects were explored by comparing the genomes of reptile-, bird-, and mammal-associated Helicobacter lineages. Based on average nucleotide identity, all reptile-associated Helicobacter lineages in this study could be considered distinct species. A whole genome-based phylogeny showed two distinct clades, one associated with chelonians and one associated with lizards. The phylogeny indicates initial adaptation to an anatomical niche, which is followed by an ancient host jump and subsequent diversification. Furthermore, the ability to grow at low temperatures, which might reflect thermal adaptation to a reptilian host, originated at least twice in Helicobacter evolution. A putative tricarballylate catabolism locus was specifically present in Campylobacter and Helicobacter isolates from reptiles. The phylogeny of reptile-associated Helicobacter parallels host association, indicating a high level of host specificity. The high diversity and deep branching within these clades supports long-term coevolution with, and extensive radiation within the respective reptilian host type.

The realization of high-performance, small-footprint, on-chip inductors remains a challenge in radio-frequency and power microelectronics, where they perform vital energy transduction in filters and power converters. Modern planar inductors consist of metallic spirals that consume significant chip area, resulting in low inductance densities. We present a novel method for magnetic energy transduction that utilizes ferromagnetic islands (FIs) on the surface of a 3D time-reversal-invariant topological insulator (TI) to produce paradigmatically different inductors. Depending on the chemical potential, the FIs induce either an anomalous or quantum anomalous Hall effect in the topological surface states. These Hall effects direct current around the FIs, concentrating magnetic flux and producing a highly inductive device. Using a novel self-consistent simulation that couples AC non-equilibrium Green functions to fully electrodynamic solutions of Maxwell's equations, we demonstrate excellent inductance densities up to terahertz frequencies, thus harnessing the unique properties of topological materials for practical device applications.

During independent diagnostic screenings of otariid seals in California (USA) and phocid seals in Scotland (UK), Campylobacter-like isolates, which differed from the established taxa of the genus Campylobacter, were cultured from abscesses and internal organs of different seal species. A polyphasic study was undertaken to determine the taxonomic position of these six isolates. The isolates were characterized by 16S rRNA gene and AtpA sequence analysis and by conventional phenotypic testing. The whole-genome sequences were determined for all isolates, and the average nucleotide identity (ANI) was determined. The isolates formed a separate phylogenetic clade, divergent from all other taxa of the genus Campylobacter and most closely related to Campylobactermucosalis. Although all isolates showed 100 % 16S rRNA gene sequence homology, AtpA and ANI analyses indicated divergence between the otariid isolates from California and the phocid isolates from Scotland, which warrants subspecies status for each clade. The two subspecies could also be distinguished phenotypically on the basis of catalase activity. This study shows clearly that the isolates obtained from pinnipeds represent a novel species within the genus Campylobacter, for which the name Campylobacter pinnipediorum sp. nov. is proposed. Within this novel species, the Californian isolates represent a separate subspecies, for which the name C. pinnipediorum subsp. pinnipediorum subsp. nov. is proposed. The type strain for both this novel species and subspecies is RM17260T (=LMG 29472T=CCUG 69570T). The Scottish isolates represent another subspecies, for which the name C. pinnipediorum subsp. caledonicus subsp. nov. is proposed. The type strain of this subspecies is M302/10/6T (=LMG 29473T=CCUG 68650T).

Campylobacter fetus currently comprises three recognized subspecies, which display distinct host association. Campylobacter fetus subsp. fetus and C fetus subsp. venerealis are both associated with endothermic mammals, primarily ruminants, whereas C fetus subsp. testudinum is primarily associated with ectothermic reptiles. Both C. fetus subsp. testudinum and C. fetus subsp. fetus have been associated with severe infections, often with a systemic component, in immunocompromised humans. To study the genetic factors associated with the distinct host dichotomy in C. fetus, whole-genome sequencing and comparison of mammal- and reptile-associated C fetus was performed. The genomes of C fetus subsp. testudinum isolated from either reptiles or humans were compared with elucidate the genetic factors associated with pathogenicity in humans. Genomic comparisons showed conservation of gene content and organization among C fetus subspecies, but a clear distinction between mammal- and reptile-associated C fetus was observed. Several genomic regions appeared to be subspecies specific, including a putative tricarballylate catabolism pathway, exclusively present in C fetus subsp. testudinum strains. Within C fetus subsp. testudinum, sapA, sapB, and sapAB type strains were observed. The recombinant locus iamABC (mlaFED) was exclusively associated with invasive C fetus subsp. testudinum strains isolated from humans. A phylogenetic reconstruction was consistent with divergent evolution in host-associated strains and the existence of a barrier to lateral gene transfer between mammal- and reptile-associated C fetus Overall, this study shows that reptile-associated C fetus subsp. testudinum is genetically divergent from mammal-associated C fetus subspecies., (© The Author 2016. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution.)

Aharonov-Bohm oscillations effectively demonstrate coherent, ballistic transport in mesoscopic rings and tubes. In three-dimensional topological insulator nanowires, they can be used to not only characterize surface states but also to test predictions of unique topological behaviour. Here we report measurements of Aharonov-Bohm oscillations in (Bi1.33Sb0.67)Se3 that demonstrate salient features of topological nanowires. By fabricating quasi-ballistic three-dimensional topological insulator nanowire devices that are gate-tunable through the Dirac point, we are able to observe alternations of conductance maxima and minima with gate voltage. Near the Dirac point, we observe conductance minima for zero magnetic flux through the nanowire and corresponding maxima (having magnitudes of almost a conductance quantum) at magnetic flux equal to half a flux quantum; this is consistent with the presence of a low-energy topological mode. The observation of this mode is a necessary step towards utilizing topological properties at the nanoscale in post-CMOS applications.

During sampling of reptiles for members of the class Epsilonproteobacteria, strains representing a member of the genus Campylobacter not belonging to any of the established taxa were isolated from lizards and chelonians. Initial amplified fragment length polymorphism, PCR and 16S rRNA sequence analysis showed that these strains were most closely related to Campylobacter fetus and Campylobacter hyointestinalis. A polyphasic study was undertaken to determine the taxonomic position of five strains. The strains were characterized by 16S rRNA and atpA sequence analysis, matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry and conventional phenotypic testing. Whole-genome sequences were determined for strains 1485E(T) and 2463D, and the average nucleotide and amino acid identities were determined for these strains. The strains formed a robust phylogenetic clade, divergent from all other species of the genus Campylobacter. In contrast to most currently known members of the genus Campylobacter, the strains showed growth at ambient temperatures, which might be an adaptation to their reptilian hosts. The results of this study clearly show that these strains isolated from reptiles represent a novel species within the genus Campylobacter, for which the name Campylobacter iguaniorum sp. nov. is proposed. The type strain is 1485E(T) ( = LMG 28143(T) = CCUG 66346(T))., (© 2015 IUMS.)

To study the effects of an acute exposure to turbidity generated by suspended sediment, we examined swimming performance (Ucrit) and related metabolic parameters in individual and groups of juvenile trout at three different concentrations of calcium carbonate. To investigate differences among strains or provenience, we compared one strain of rainbow trout (Oncorhynchus mykiss; RBT) and one strain of brown trout (Salmo trutta; BNT) from a common hatchery and one RBT strain from a separate hatchery. In general, trout swum individually or in groups exhibited a decrease in Ucrit as turbidity increased. Both RBT strains were more similar to each other and were impaired to a larger extent in swimming performance than BNT, which was less impacted. For groups, indicators of aerobic metabolism were elevated while those of anaerobic metabolism were depressed. Specifically, citrate synthase activities and glucose levels tended to be greater while plasma lactate and LDH activities were reduced. Lactate and LDH levels in individually swum trout under sediment exposure suggest a greater similarity of fish from the same provenience. We suggest that acute exposures to environmentally relevant turbidities generated by fine suspended sediment may cause a reduced Ucrit, and that these changes may be related to changes in the utilization of aerobic and anaerobic pathways., (Copyright © 2014 Elsevier Inc. All rights reserved.)

We explore the 32 crystallographic point groups and identify topological phases of matter with robust surface modes. For n=3,4, and 6 of the C_{nv} groups, we find the first-known 3D topological insulators without spin-orbit coupling, and with surface modes that are protected only by point groups; i.e., the relevant symmetries are purely crystalline and do not include time reversal. To describe these C_{nv} systems, we introduce the notions of (a) a halved mirror chirality, an integer invariant which characterizes half-mirror-planes in the 3D Brillouin zone, and (b) a bent Chern number, the traditional Thouless-Kohmoto-Nightingale-den Nijs invariant generalized to bent 2D manifolds. We find that a Weyl semimetallic phase intermediates two gapped phases with distinct halved chiralities. In addition to electronic systems without spin-orbit coupling, our findings also apply to intrinsically spinless systems such as photonic crystals and ultracold atoms.

A polyphasic study was undertaken to determine the taxonomic position of 13 Campylobacter fetus-like strains from humans (n = 8) and reptiles (n = 5). The results of matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) MS and genomic data from sap analysis, 16S rRNA gene and hsp60 sequence comparison, pulsed-field gel electrophoresis, amplified fragment length polymorphism analysis, DNA-DNA hybridization and whole genome sequencing demonstrated that these strains are closely related to C. fetus but clearly differentiated from recognized subspecies of C. fetus. Therefore, this unique cluster of 13 strains represents a novel subspecies within the species C. fetus, for which the name Campylobacter fetus subsp. testudinum subsp. nov. is proposed, with strain 03-427(T) ( = ATCC BAA-2539(T) = LMG 27499(T)) as the type strain. Although this novel taxon could not be differentiated from C. fetus subsp. fetus and C. fetus subsp. venerealis using conventional phenotypic tests, MALDI-TOF MS revealed the presence of multiple phenotypic biomarkers which distinguish Campylobacter fetus subsp. testudinum subsp. nov. from recognized subspecies of C. fetus.

Campylobacter iguaniorum has been isolated from reptiles. This Campylobacter species is genetically related to Campylobacter fetus and Campylobacter hyointestinalis. Here we present the first whole-genome sequence for this species., (Copyright © 2014 Gilbert et al.)

Campylobacter, Arcobacter, and Helicobacter species have been isolated from many vertebrate hosts, including birds, mammals, and reptiles. Multiple studies have focused on the prevalence of these Epsilonproteobacteria genera in avian and mammalian species. However, little focus has been given to the presence within reptiles, and their potential zoonotic and pathogenic roles. In this study, occurrence, diversity, and host association of intestinal Epsilonproteobacteria were determined for a large variety of reptiles. From 2011 to 2013, 444 cloacal swabs and fecal samples originating from 417 predominantly captive-held reptiles were screened for Epsilonproteobacteria. Campylobacter, Arcobacter, and Helicobacter genus specific PCRs were performed directly on all samples. All samples were also cultured on selective media and screened for the presence of Epsilonproteobacteria. Using a tiered approach of AFLP, atpA, and 16S rRNA sequencing, 432 Epsilonproteobacteria isolates were characterized at the species level. Based on PCR, Campylobacter, Arcobacter, and Helicobacter were detected in 69.3% of the reptiles; 82.5% of the chelonians, 63.8% of the lizards, and 58.0% of the snakes were positive for one or more of these genera. Epsilonproteobacteria were isolated from 22.1% of the reptiles and were isolated most frequently from chelonians (37.0%), followed by lizards (19.6%) and snakes (3.0%). The most commonly isolated taxa were Arcobacter butzleri, Arcobacter skirrowii, reptile-associated Campylobacter fetus subsp. testudinum, and a putative novel Campylobacter taxon. Furthermore, a clade of seven related putative novel Helicobacter taxa was isolated from lizards and chelonians. This study shows that reptiles carry various intestinal Epsilonproteobacteria taxa, including several putative novel taxa.

We study a new type of three-dimensional topological superconductor that exhibits Majorana zero modes (MZM) protected by a magnetic group symmetry, a combined antiunitary symmetry composed of a mirror reflection and time reversal. This new symmetry enhances the noninteracting topological classification of a superconducting vortex from Z2 to Z, indicating that multiple MZMs can coexist at the end of one magnetic vortex of unit flux. Especially, we show that a vortex binding two MZMs can be realized on the (001) surface of a topological crystalline insulator SnTe with proximity induced BCS Cooper pairing, or in bulk superconductor InxSn1-xTe.

Mutation of Glass bottom boat, the Drosophila homologue of the bone morphogenetic protein or growth/differentiation factor (BMP/GDF) family of genes in vertebrates, has been shown to disrupt development of neuromuscular junctions (NMJ). Here we tested whether this same conclusion can be broadened to vertebrate BMP/GDF genes. This analysis was also extended to consider whether such genes are required for NMJ maintenance in post-larval stages, as this would argue that BMP genes are viable candidates for analysis in progressive neuromuscular disease. Zebrafish mutants harboring homozygous null mutations in the BMP-family gene gdf6a were raised to adulthood and assessed for neuromuscular deficits. Fish lacking gdf6a exhibited decreased endurance (∼ 50%, p = 0.005) compared to wild type, and this deficit progressively worsened with age. These fish also presented with significantly disrupted NMJ morphology (p = 0.009), and a lower abundance of spinal motor neurons (∼ 50%, p<0.001) compared to wild type. Noting the similarity of these symptoms to those of Amyotrophic Lateral Sclerosis (ALS) model mice and fish, we asked if mutations in gdf6a would enhance the phenotypes observed in the latter, i.e. in zebrafish over-expressing mutant Superoxide Dismutase 1 (SOD1). Amongst younger adult fish only bigenic fish harboring both the SOD1 transgene and gdf6a mutations, but not siblings with other combinations of these gene modifications, displayed significantly reduced endurance (75%, p<0.05) and strength/power (75%, p<0.05), as well as disrupted NMJ morphology (p<0.001) compared to wild type siblings. Bigenic fish also had lower survival rates compared to other genotypes. Thus conclusions regarding a role for BMP ligands in effecting NMJ can be extended to vertebrates, supporting conservation of mechanisms relevant to neuromuscular degenerative diseases. These conclusions synergize with past findings to argue for further analysis of GDF6 and other BMP genes as modifier loci, potentially affecting susceptibility to ALS and perhaps a broader suite of neurodegenerative diseases.

A rapidly aging global population has motivated the development and use of models for human aging. Studies on aging have shown parallels between zebrafish and humans at the internal organization level; however, few parallels have been studied at the whole-organism level. Furthermore, the effectiveness of exercise as a method to mitigate the effects of aging has not been studied in zebrafish. We investigated the effects of aging and intermittent exercise on swimming performance, kinematics and behavior. Young, middle-aged and old zebrafish (20-29, 36-48 and 60-71% of average lifespan, respectively) were exercised to exhaustion in endurance and sprint swimming tests once a week for four weeks. Both endurance and sprint performance decreased with increased age. Swimming performance improved with exercise training in young and middle-aged zebrafish, but not in old zebrafish. Tail-beat amplitude, which is akin to stride length in humans, increased for all age groups with training. Zebrafish turning frequency, which is an indicator of routine activity, decreased with age but showed no change with exercise. In sum, our results show that zebrafish exhibit a decline in whole-organism performance and trainability with age. These findings closely resemble the senescence-related declines in physical ability experienced by humans and mammalian aging models and therefore support the use of zebrafish as a model for human exercise and aging., (Copyright © 2013 The Authors. Published by Elsevier Inc. All rights reserved.)

We theoretically predict that thin-film topological crystalline insulators can host various quantum anomalous Hall phases when doped by ferromagnetically ordered dopants. Any Chern number between ±4 can, in principle, be reached as a result of the interplay between (a) the induced Zeeman field, depending on the magnetic doping concentration, (b) the structural distortion, either intrinsic or induced by a piezoelectric material through the proximity effect, and (c) the thickness of the thin film. We propose a heterostructure to realize quantum anomalous Hall phases with Chern numbers that can be tuned by electric fields.

Campylobacter fetus subsp. testudinum has been isolated from reptiles and humans. This Campylobacter subspecies is genetically distinct from other C. fetus subspecies. Here, we present the first whole-genome sequence for this C. fetus subspecies.

Campylobacter fetus subsp. testudinum subsp. nov. is a newly proposed subspecies of C. fetus with markers of reptile origin. We summarize epidemiologic information for 9 humans infected with this bacterium. All cases were in men, most of whom were of Asian origin. Infection might have been related to exposure to Asian foods or reptiles.

Coupling the surface state of a topological insulator to an s-wave superconductor is predicted to produce the long-sought Majorana quasiparticle excitations. However, superconductivity has not been measured in surface states when the bulk charge carriers are fully depleted, that is, in the true topological regime relevant for investigating Majorana modes. Here we report measurements of d.c. Josephson effects in topological insulator-superconductor junctions as the chemical potential is moved through the true topological regime characterized by the presence of only surface currents. We compare our results with three-dimensional quantum transport simulations, and determine the effects of bulk/surface mixing, disorder and magnetic field; in particular, we show that the supercurrent is largely carried by surface states, due to the inherent topology of the bands, and that it is robust against disorder. Our results thus clarify key open issues regarding the nature of supercurrents in topological insulators.

Ultrasonic backscatter techniques may offer a useful approach for detecting changes in cancellous bone caused by osteoporosis and other diseases. The goal of this study was to investigate the utility of a backscatter difference technique for ultrasonic bone assessment. Measurements were performed on 22 cube-shaped specimens of human cancellous bone using four broadband transducers with center frequencies 2.25, 5, 7.5, and 10 MHz. The backscatter difference spectrum D(f) was obtained by subtracting power spectra (in dB) from two different portions of the same backscatter signal. D(f) was found to be a monotonically increasing, quasi-linear function of frequency when averaged over multiple measurement sites on multiple specimens. The frequency slope of D(f) demonstrated weak to moderate correlations with specimen density (R = 0.21-0.80). The frequency averaged mean of D(f) demonstrated moderate to good correlations with density (R = 0.70-0.95). These results suggest that parameters based on the frequency averaged mean of the backscatter difference spectrum may be useful for bone assessment purposes.

We study the effects of disorder on the interlayer transport properties of disordered semiconductor bilayers by performing self-consistent quantum transport calculations. We find that the addition of material disorder to the system affects the interlayer interactions leading to significant deviations in the interlayer transfer characteristics. In particular, we find that disorder decreases and broadens the tunneling peak, effectively reducing the interacting system to a non-interacting system. Our results suggest that the experimental observation of exchange-enhanced interlayer transport in semiconductor bilayers requires materials with mean free paths larger than the spatial extent of the system.

Peptidyl-tRNA Hydrolase (Pth) is a highly conserved, essential enzyme in bacteria. It removes the peptide portion from peptidyl-tRNA, returning free tRNAs to participate in translation. Build-up of peptidyl-tRNAs is toxic and defects in Pth function result in cell death. Herein we use in vitro activity of recombinant E. coli Pth to screen tropical plant extracts for inhibition. Multiple extracts were found to have inhibitory activity with some exhibiting different inhibitory effects depending on extraction conditions. IC50 values ranged from 0.02 to > 53.8 microg of extract per 1 unit of Pth, holding promise for in vivo screening. The inhibitory components in these extracts may serve as lead compounds for development of novel antibacterials.

We perform a complete classification of two-band k·p theories at band crossing points in 3D semimetals with n-fold rotation symmetry and broken time-reversal symmetry. Using this classification, we show the existence of new 3D topological semimetals characterized by C(4,6)-protected double-Weyl nodes with quadratic in-plane (along k(x,y)) dispersion or C(6)-protected triple-Weyl nodes with cubic in-plane dispersion. We apply this theory to the 3D ferromagnet HgCr(2)Se(4) and confirm it is a double-Weyl metal protected by C(4) symmetry. Furthermore, if the direction of the ferromagnetism is shifted away from the [001] axis to the [111] axis, the double-Weyl node splits into four single Weyl nodes, as dictated by the point group S(6) of that phase. Finally, we discuss experimentally relevant effects including the splitting of multi-Weyl nodes by applying a C(n) breaking strain and the surface Fermi arcs in these new semimetals.

We investigate the superfluid properties of disordered double layer graphene systems using the non-equilibrium Green's function formalism. The complexity of such a structure makes it imperative to study the effects of lattice vacancies which will inevitably arise during fabrication. We present and compare room temperature performance characteristics for both ideal and disordered double layer graphene systems in an effort to illustrate the behavior of a Bose-Einstein condensate in the presence of lattice defects under non-equilibrium conditions. We find that lattice vacancies spread throughout the top layer past the coherence length have a reduced effect compared to the ideal case. However, vacancies concentrated near the metal contacts within the coherence length significantly alter the interlayer superfluid transport properties.

We explore exchange coupling of a pair of spins in a double dot and in an optical lattice, using the frequency of exchanges in a bosonic path integral, evaluated using Monte Carlo simulation. The algorithm gives insights into the role of correlation through visualization of two-particle probability densities, instantons, and the correlation hole. We map the problem to the Hubbard model and see that exchange and correlation renormalize the model parameters, dramatically reducing the effective on-site repulsion at larger separations., (© 2010 IOP Publishing Ltd)

The American Academy of Pediatrics recommends counseling about obesity-specific topics and measuring body mass index (BMI) at all health supervision visits. The authors compared the rates of overweight diagnosis and physician counseling at 2 clinics, one using color-coded BMI charts. The authors interviewed patients and reviewed their charts for differences in diagnosis and counseling rates. They also retrospectively compared age- and physician-matched charts pre- and postintervention, reviewing them for diagnosis of overweight. Intervention patients were more likely to have their BMI discussed (53/77 vs 3/70, P < .0001) and be identified as overweight both prospectively (8/10 vs 1/8, P = .015) and in the authors' matched chart review (8/10 vs 1/10, P = .005). Discussion rates of obesity-related topics such as nutrition, physical activity, and media time did not differ. BMI charts increase overweight patient identification by pediatricians. Further research is needed on the benefits of earlier overweight identification and best counseling methods for promoting healthy weight.

Objective: The American Academy of Pediatrics has issued a primary pediatric obesity prevention policy statement. Its focus is directed toward the health supervision visit. We used qualitative research to determine physicians' approaches toward obesity prevention within this visit., Design: Twenty-four University of Wisconsin pediatricians participated in a qualitative study consisting of data transcribed from audio-taped interviews. Open-ended questions investigated specific health supervision practices pertaining to obesity prevention, and major themes were identified., Results: The pediatrician's role in obesity prevention is education and detection. Pediatricians provide information on proper nutrition, physical activity, media time, and parenting skills. These pediatricians routinely discuss (1) junk food, (2) balanced diets, (3) nutritional requirements, and (4) parental techniques to promote healthy approaches to food. They discuss methods to increase physical activity and routinely recommend limitations to media time. However, only a third use body mass index (BMI) charts during their health supervision appointments and many are uncertain how to tailor guidance to children with obesity risk factors., Conclusions: Pediatricians follow many of the obesity prevention guidelines. Further evidence is needed to understand the effectiveness of their education and detection methods. Potential areas for improvement include use of BMI charts and counseling with specific obesity prevention in mind.

To cause diseases in plants, pathogenic microorganisms have evolved mechanisms to deliver proteins directly into plant cells, where they suppress plant defences and facilitate tissue invasion. How plant pathogenic fungi, which cause many of the world's most serious plant diseases, deliver proteins during plant infection is currently unknown. Here we report the characterization of a P-type ATPase-encoding gene, MgAPT2, in the economically important rice blast pathogen Magnaporthe grisea, which is required for exocytosis during plant infection. Targeted gene replacement showed that MgAPT2 is required for both foliar and root infection by the fungus, and for the rapid induction of host defence responses in an incompatible reaction. DeltaMgapt2 mutants are impaired in the secretion of a range of extracellular enzymes and accumulate abnormal Golgi-like cisternae. However, the loss of MgAPT2 does not significantly affect hyphal growth or sporulation, indicating that the establishment of rice blast disease involves the use of MgApt2-dependent exocytotic processes that operate during plant infection.

Purpose: Troxacitabine is the first unnatural L-nucleoside analog to show potent preclinical antitumor activity and is currently under clinical investigation. Significant differences in troxacitabine toxicity between mice, rats, monkeys, and humans were observed during preclinical and clinical evaluations. To better understand the different toxicity and efficacy results observed between the human xenograft mouse tumor models used for preclinical assessment and the clinical study results, the pharmacodynamics and pharmacokinetics of troxacitabine were reassessed in murine and human models., Experimental Design: Clonal and thymidine incorporation assays were used to investigate the in vitro antiproliferative activity of troxacitabine on a selected panel of mouse and human tumor cell lines and normal hemapoietic cells. Analysis of the intracellular metabolites of [14C]troxacitabine was determined in mouse and human T-lymphocytes obtained from peripheral blood. The antitumor efficacy of troxacitabine administered either as single or repeated high-dose bolus administrations or as low-dose continuous infusions was evaluated in the human colon HT-29 xenograft model. We also determined plasma concentrations of troxacitabine using the different administration schedules., Results: Five to nine hundred-fold lower concentrations of troxacitabine were required to inhibit cell growth in human compared with murine tumor and normal hemapoietic cell lines. Furthermore, the sensitivity of cells of both species to troxacitabine was strongly time dependent, requiring >24 hours exposure for maximum activity. Analysis of the intracellular metabolites of [14C]troxacitabine in T-lymphocytes obtained from peripheral blood revealed subsequently higher levels of mono-, di-, and triphosphates in human compared with mouse. Antitumor efficacy studies revealed that prolonged exposure schedules (up to 6 days) showed equivalent efficacy to repeated high-dose bolus administrations. Five-day continuous infusion of 20 mg/mL troxacitabine via subcutaneous implanted mini-osmotic pump maintained systemic concentrations of 262 ng/mL (1.2 micromol/L) for the duration of administration, which are clinically achievable plasma concentrations, and led to significant antitumor activity [treated versus control (T/C) of 27% and tumor regression during treatment]., Conclusions: These studies support the hypothesis that troxacitabine infusions might be the administration regimen with the greatest likelihood of fully exploiting clinically the potent preclinical antitumor activity of troxacitabine.

Background: Studies have reported that plasma human immunodeficiency virus type 1 (HIV-1) RNA levels and CD4+ lymphocyte counts in HIV-infected patients improved after treatment with granulocyte-macrophage colony-stimulating factor (GM-CSF)., Methods: In AIDS Clinical Trials Group Protocol 5041, 116 patients were enrolled in a double-blind, randomized, placebo-controlled clinical trial of 16 weeks of 250 microg of GM-CSF administered subcutaneously 3 times/week, followed by open-label treatment for an additional 32 weeks. Patients had stable baseline plasma HIV-1 RNA levels of > or =1500 copies/mL and received constant antiretroviral regimens through at least the first 16 weeks of the study., Results: After 16 weeks, the GM-CSF group tended to have greater, though clinically insignificant, increases in plasma HIV-1 RNA levels, compared with the placebo group (median change, +0.048 vs. -0.103 log copies/mL; P=.036, in a post hoc analysis). There were trends toward progressive modest increases in CD4+ lymphocyte counts with GM-CSF treatment at 16 weeks (median change, +14 vs. -6 cells/mm3; P=.06) and beyond., Conclusions: GM-CSF does not have an antiviral effect in patients with ongoing HIV replication but may increase CD4+ lymphocyte counts.

Powdery mildew fungi are biotrophic pathogens that form a complex interface, the haustorium, between the host plant and the parasite. The pathogen acts as an additional sink, competing with host sinks, resulting in considerable modification of photoassimilate production and partitioning within the host tissue. Here, we examine the factors that may contribute to these changes. We show for the first time in one biotrophic interaction (Arabidopsis/Erysiphe cichoracearum) all of the following responses: Glc uptake in host tissues is enhanced after fungal infection; this coincides with the induction of expression of the monosaccharide transporter gene, Arabidopsis sugar transport protein 4 (AtSTP4), in infected leaves; invertase activity and transcript levels for a cell wall invertase, Atbetafruct1, increase substantially in Arabidopsis during attack by this pathogen. Before infection, Arabidopsis plants transformed with an AtSTP4 promoter-beta-glucuronidase construct show expression mainly in sink tissues such as roots; after infection, AtSTP4 expression is induced in the mature leaves and increases over the 6-d time period. Sections of infected leaves stained for beta-glucuronidase show that AtSTP4 expression is not confined to infected epidermal cells but is also evident in a wider range of cells, including those of the vascular tissue. The results are discussed in relation to the possible coordinated expression of hexose transporters and cell wall invertase in the host response to powdery mildew infection.