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92 results on '"Gimenez-Gallego, Guillermo"'

7. Hints of nonhierarchical folding of acidic fibroblast growth factor

Author

Sanz, Jesus M., Jimenez, M. Angeles, and Gimenez-Gallego, Guillermo

Subjects
Biochemistry -- Research, Growth factors -- Physiological aspects, Fibroblasts -- Physiological aspects, Acids -- Physiological aspects, Biological sciences, Chemistry
Abstract

Research has been conducted on the all-beta protein acidic fibroblast growth factor and the peptides involved in the receptor binding. The helical propensity of the growth factor and the peptides has been investigated via the use of the circular dichroism and proton nuclear magnetic resonance.

Published
2002

8. Partial IGF affinity of circulating N- and C-terminal fragments of human insulin-like growth factor binding protein-4 (IGFBP-4) and the disulfide bonding pattern of the C-terminal IGFBP-4 domain

Author

Standker, Ludger, Braulke, Thomas, Mark, Silke, Mostafavi, Hossein, Meyer, Markus, Honing, Stefan, Gimenez-Gallego, Guillermo, and Forssmann, Wolfe-Georg

Subjects
Biochemistry -- Research, Sulfides -- Research, Insulin -- Research, Growth factors -- Analysis, Carrier proteins -- Research, Chemical bonds -- Analysis, Biological sciences, Chemistry
Abstract

Research has been conducted on the insulin-like growth factor-binding proteins. Results include molecular characterization of circulating human IGFBP-4 fragments that have been formed in vivo.

Published
2000

9. (super)1 H NMR structural characterization of a nonmitogenic, vasodilatory, ischemia-protector and neuromodulatory acidic fibroblast growth factor

Author

Lozano, Rosa M., Pineda-Lucena, Antonio, Gonzalez, Carlos, Jimenez, M. Angeles, Cuevas, Pedro, Redondo-Horcajo, Mariano, Sanz, Jesus M., Rico, Manuel, and Gimenez-Gallego, Guillermo

Subjects
Biochemistry -- Research, Mitogens -- Research, Vasodilators -- Analysis, Ischemia -- Causes of, Fibroblast growth factors -- Research, Amino acids -- Research, Biological sciences, Chemistry
Abstract

Research has been conducted on the acidic fibroblast growth factor (aFGF). The vasodilatory activity of the aFGF has been investigated.

Published
2000

10. Correction of hypertension by normalization of endothelial levels in fibroblast growth factor and nitric oxide synthase in spontaneously hypertensive rats

Author

Cuevas, Pedro, Garcia-Calvo, Margarita, Carceller, Fernando, Reimers, Diana, Zazo, Mercedes, Cuevas, Begona, Munoz-Willery, Isabel, Martinez-Coso, Victoria, Lamas, Santiago, and Gimenez-Gallego, Guillermo

Subjects
Hypertension -- Physiological aspects, Fibroblast growth factors -- Physiological aspects, Endothelium -- Physiological aspects, Rats -- Physiological aspects, Science and technology
Abstract

Acidic and basic fibroblast growth factors (FGFs) share a wide range of diverse biological activities. To date, low levels of FGF have not been correlated with a pathophysiologic state. We report that blood vessels of spontaneously hypertensive rats are shown to be associated with a marked decrement in endothelial basic FGF content. This decrement correlates both with hypertension and with a decrease in the endothelial content of nitric oxide synthase. Restoration of FGF to physiological levels in the vascular wall, either by systemic administration or by in vivo gene transfer, significantly augmented the number of endothelial cells with positive immunostaining for nitric oxide synthase, corrected hypertension, and ameliorated endothelial-dependent responses to vasoconstrictors. These results suggest an important role for FGFs in blood pressure homeostasis and open new avenues for the understanding of the etiology and treatment of hypertension.

Published
1996

13. Intravitreal Dobesilate Injection for Macular Oedema Secondary to Branch Retinal Vein Occlusion

Author

Outeiriño Luis Antonio, Angulo Javier, Azanza Carlos, Cuevas Sanchez Pedro, and Gimenez-Gallego Guillermo

Subjects
medicine.medical_specialty, Central retinal vein, Visual acuity, genetic structures, business.industry, Macular oedema, Retinal, General Medicine, medicine.disease, eye diseases, chemistry.chemical_compound, medicine.anatomical_structure, chemistry, Ophthalmology, Occlusion, medicine, Branch retinal vein occlusion, sense organs, medicine.symptom, business, Vein, Retinal haemorrhage
Abstract

We describe a patient with macular oedema secondary to occlusion of two branches of the central retinal vein, who has been treated with a single intravitreal injection of dobesilate. Three months after treatment, visual acuity reached 1, with an anatomical resolution of the macular oedema and the retinal haemorrhage. To our knowledge this is the first case reporting a resolution of macular oedema associated to branch retinal vein occlusions after a single intravitreal injection of dobesilate.

Published
2016

14. Hypotensive activity of fibroblast growth factor

Author

Cuevas, Pedro, Carceller, Fernando, Ortega, Sagrario, Zazo, Mercedes, Nieto, Ignacio, and Gimenez-Gallego, Guillermo

Subjects
Blood vessels -- Dilatation, Antihypertensive drugs -- Research, Fibroblast growth factors -- Research, Science and technology, Research
Abstract

Acidic and basic fibroblast growth factors (FGFs) are members of a family of proteins that are broad-spectrum mitogens, have diverse hormone-like activities, and function in tumorigenesis. FGF's ability to raise the concentration of intracellular calcium ion suggests that FGF could induce the synthesis of endothelium-derived relaxing factor (EDRF) and consequently vasodilation. Systemic administration of FGF decreased arterial blood pressure. This effect was mediated by EDRF and by adenosine triphosphate--sensitive potassium ion channels. The hypotensive effect of FGF was segregated from its mitogenic activity by protein engineering. These results extend the range of FGF autocrine activities and potential therapeutic applications, emphasize the role of endothelium as an arterial blood pressure--regulating organ, and provide insight on the structural basis of FGF functions., THE PROTEINS OF THE FGF FAMILY are broad spectrum mitogens synthesized in vertebrates in tissues derived from mesoderm and neuroectoderm [1]. However, basic FGF (bFGF) also has non-mitogenic activities [2, [...]

Published
1991

15. Conformational flexibility of a synthetic glycosylaminoglycan bound to a fibroblast growth factor. FGF-1 recognizes both the (super 1)C(sub 4) and (super 2)S(sub 0) conformations of a bioactive heparin-like hexasaccharide

Author

Canales, Angeles, Angulo, Jesus, Ojeda, Rafael, Bruix, Marta, Fayos, Rosa, Lozano, Rosa, Gimenez-Gallego, Guillermo, Martin-Lomas, Manuel, Nieto, Pedro M., and Jimenez-Barbero, Jesus

Subjects
Fibroblast growth factors -- Research, Polypeptides -- Atomic properties, Polypeptides -- Structure, Conformational analysis, Chemistry
Abstract

A study is conducted which describes the biological, structural, and conformational details of the binding of glycosylaminoglycans (GAGs) to polypeptides of the fibroblast growth factor (FGF) family, the chemokines, and to antithrombin-III (AT-III). The results obtained indicate that within the complex with FGF-1, the iduronate rings of the bound oligosaccharide display a conformational equilibrium between the (super 1)C(sub 4) and (super 2)S(sub 0) forms.

Published
2005

16. Solution structure of RicC3, a 2S albumin storage protein from Ricinus communis

Author

Pantoja-Uceda, David, Bruix, Marta, Gimenez-Gallego, Guillermo, Rico, Manuel, and Santoro, Jorge

Subjects
Albumin -- Structure, Castor beans -- Research, Castor oil plant -- Research, Structure-activity relationships (Biochemistry) -- Analysis, Proteins -- Analysis, Proteins -- Structure, Biological sciences, Chemistry
Abstract

Research describes the three-dimensional solution structure of recombinant RicC3 albumin protein from the castor beans. The core structure display a RMSD radius of 0.64 Angstroms. The nuclear magnetic resonance structure shows five amphipatic helices in a right-handed super helix, unique to this lipid transfer protein. r

Published
2003

19. Brain-derived acidic fibroblast growth factor: complete amino acid sequence and homologies

Author

Gimenez-Gallego, Guillermo

Subjects
Biochemistry -- Research, Amino acids -- Research, Fibroblasts -- Research, Science and technology, Research
Abstract

Brain-Derived Acidic Fibroblast Growth Factor: Complete Amino Acid Sequence and Homologies Brain has been recognized as a plentiful source of mitogenic activity for primary fibroblasts for almost 50 years (1). [...]

Published
1985

33. Acidic Fibroblast Growth Factor Accelerates Dermal Wound Healing

Author

Mellin, Theodore N., primary, Mennie, Robert J., additional, Cashen, Doreen E., additional, Ronan, John J., additional, Capparella, Joanna, additional, James, Mary Lou, additional, Disalvo, Jerry, additional, Frank, John, additional, Linemeyer, David, additional, Gimenez-Gallego, Guillermo, additional, and Thomas, Kenneth A., additional

Published
1992
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36. Photooxidase System of <em>Rhodospirillum rubrum</em> III. The Role of Rhodoquinone and Ubiquinone in the Activity of Preparations of Chromatophores and Photoreaction Centers.

Author

Gimenez-Gallego, Guillermo, Ramírez-Ponce, M. Pilar, Lauzurica, Pilar, and Ramírez, Juan M.

Subjects
*EPITHELIAL cells, *ORGANIC compounds, *ORGANIC solvents, *PLASTICIZERS, *CATHODE rays, *UBIQUINONES
Abstract

The role of rhodoquinone and ubiquinone in the oxygen photoreducing (photooxidase) activity of Rhodospirillum rubrum was investigated. The sole addition of purified rhodoquinone restored photooxidase activity in isolated chromatophores which had been extracted with organic solvents and which were apparently free of secondary acceptor ubiquinone. Rhodoquinone also enhanced photooxidase activity in photoreaction center preparations from which secondary ubiquinone seemed to have been removed. Those results suggest that rhodoquinone accepts electrons directly from primary ubiquinone during chromatophore photooxidase activity. In contrast, rhodoquinone does not participate in the basal activity of photoreaction center preparations, which seems to result from the autooxidation of both primary and secondary ubiquinone. [ABSTRACT FROM AUTHOR]

Published
1982
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37. Expression in Escherichia coli of a Chemically Synthesized Gene for Biologically Active Bovine Acidic Fibroblast Growth Factor

Author

Linemeyer, David L., Kelly, Linda J., Menke, John G., Gimenez-Gallego, Guillermo, DiSalvo, Jerry, and Thomas, Kenneth A.

Abstract

A gene encoding bovine acidic fibroblast growth factor has been chemically synthesized, cloned and expressed as a biologically active protein in Escherichia coli. The 440 base pair gene was assembled by enzymatic ligation of 16 oligonucleotides and cloned into a pBR322–derived expression plasmid downstream of the hybrid tac promoter. Expressed recombinant mitogen comigrated with the native bovine brain–derived protein as detected by Western blot immunological analysis. The expression product of the synthetic gene has been purified to apparent homogeneity and demonstrates a mitogenic activity for Balb/c 3T3 cells in the presence of heparin equivalent to the brain–derived mitogen. These results demonstrate the feasibility of expressing large amounts of functional acidic fibroblast growth factor in bacteria and provide a system for site–specific mutagenesis of the protein.

Published
1987
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38. Protection of Rat Myocardium by Mitogenic and Non-Mitogenic Fibroblast Growth Factor During Post-Ischemic Reperfusion

Author

Cuevas, Pedro, Carceller, Fernando, Lozano, Rosa Maria, Crespo, Ana, Zazo, Mercedes, and Gimenez-Gallego, Guillermo

Abstract

The effects of acidic fibroblast growth factor (FGF-1) and basic fibroblast growth factor (FGF-2) and a non mitogenic form of FGF1 on myocardial ischemia and reperfusion were assessed. Rats underwent 10 minutes of coronary artery occlusion followed by 24 hours of reperfusion. Creatinine kinase content of the affected myocardium showed that both fibroblast growth factors 1 and 2 effectively protected against ischemia reperfusion injury (p < 0.01), and that the vasoactive but nonmitogenic form of the FGF1 was equally protective (p < 0.01 versus control + vehicle). The results were confirmed by light and electron-microscopy histological studies. Histological evaluations after treatment with the non-mitogenic fibroblast growth factor 1 showed that it did not generate the severe hyperplasia and connective tissue disorganization observed with the native mitogenic proteins.The possibility of using a non-mitogenic form of fibroblast growth factor for cardio-protection circumvents many of the potentially undesirable effects that may derive from systemically introducing broad spectrum acting fibroblast growth factors in vivo. This myocardial protection observed 24 hours after the treatment with fibroblast growth factors, and the efficacy of the non-mitogenic form of the protein, also suggest that the protective effect of fibroblast growth factors may be due to the increased blood flow rather than to angiogenesis.

Published
1997
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