Search

Your search keyword '"Gina C. Kim"' showing total 6 results
Start Over Author "Gina C. Kim"
6 results on '"Gina C. Kim"'

1. Functional cardiac fibroblasts derived from human pluripotent stem cells via second heart field progenitors

Author

Jianhua Zhang, Ran Tao, Katherine F. Campbell, Juliana L. Carvalho, Edward C. Ruiz, Gina C. Kim, Eric G. Schmuck, Amish N. Raval, André Monteiro da Rocha, Todd J. Herron, José Jalife, James A. Thomson, and Timothy J. Kamp

Subjects
Science
Abstract

Cardiac fibroblasts (CFs) play critical roles in heart development, homeostasis, and disease. Here the authors efficiently differentiate human pluripotent stem cells through second heart field progenitors to CFs that exhibit features and functional properties similar to native CFs.

Published
2019
Full Text
View/download PDF

2. Cardiac differentiation of human pluripotent stem cells using defined extracellular matrix proteins reveals essential role of fibronectin

Author

Jianhua Zhang, Zachery R Gregorich, Ran Tao, Gina C Kim, Pratik A Lalit, Juliana L Carvalho, Yogananda Markandeya, Deane F Mosher, Sean P Palecek, and Timothy J Kamp

Subjects
extracellular matrix, fibronectin, mesoderm, cardiac differentiation, pluripotent stem cells, integrin linked kinase, Medicine, Science, Biology (General), QH301-705.5
Abstract

Research and therapeutic applications using human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) require robust differentiation strategies. Efforts to improve hPSC-CM differentiation have largely overlooked the role of extracellular matrix (ECM). The present study investigates the ability of defined ECM proteins to promote hPSC cardiac differentiation. Fibronectin (FN), laminin-111, and laminin-521 enabled hPSCs to attach and expand. However, only addition of FN promoted cardiac differentiation in response to growth factors Activin A, BMP4, and bFGF in contrast to the inhibition produced by laminin-111 or laminin-521. hPSCs in culture produced endogenous FN which accumulated in the ECM to a critical level necessary for effective cardiac differentiation. Inducible shRNA knockdown of FN prevented Brachyury+ mesoderm formation and subsequent hPSC-CM generation. Antibodies blocking FN binding integrins α4β1 or αVβ1, but not α5β1, inhibited cardiac differentiation. Furthermore, inhibition of integrin-linked kinase led to a decrease in phosphorylated AKT, which was associated with increased apoptosis and inhibition of cardiac differentiation. These results provide new insights into defined matrices for culture of hPSCs that enable production of FN-enriched ECM which is essential for mesoderm formation and efficient cardiac differentiation.

Published
2022
Full Text
View/download PDF

5. Low-Dose Irradiation Can be Used as a Phytosanitary Treatment for Fresh Table Grapes

Author

Fred Caporaso, Anuradha Prakash, Gina C. Kim, and Cyril Rakovski

Subjects
Chemistry, fungi, food and beverages, Berry, Sweetness, Low dose irradiation, Sensory analysis, Horticulture, Food science, Irradiation, Vitis vinifera, Flavor, Food Science, Phytosanitary certification
Abstract

Grapes (Vitis vinifera var. Sugraone and Vitis labrusca var. Crimson Seedless) were treated with 400, 600, and 800 Gy and the effects on physicochemical factors were measured alongside sensory testing during 3 wk of storage. Significant changes in texture and color with irradiation and age were measured but little visual difference was seen between control and irradiated grapes. However, age had a greater effect on firmness than irradiation for Sugraone grapes. Irradiation did not significantly (P ≤ 0.05) affect the SSC/TA ratio, which increased during storage. The trained panel detected significant changes in the berry texture and rachis color but rated sweetness and flavor significantly higher (P ≤ 0.05) for irradiated Sugraone as compared to the control. Consumers liked both the untreated and 800 Gy treated Sugraone grapes, but liked the untreated grapes more for texture (P ≤ 0.05). However, there was no difference in liking between irradiated (600 Gy or 800 Gy) and control samples of Crimson Seedless for any attribute. The results show that there are varietal differences in response to irradiation but the overall maintenance in quality of irradiated grapes during 3 wk of storage indicates that irradiation can serve as a viable phytosanitary treatment.

Published
2014

6. Low-dose irradiation can be used as a phytosanitary treatment for fresh table grapes

Author

Gina C, Kim, Cyril, Rakovski, Fred, Caporaso, and Anuradha, Prakash

Subjects
Anthocyanins, Disinfection, Fruit, Taste, Food Irradiation, Food Quality, Color, Humans, Dose-Response Relationship, Radiation, Vitis, Consumer Behavior
Abstract

Grapes (Vitis vinifera var. Sugraone and Vitis labrusca var. Crimson Seedless) were treated with 400, 600, and 800 Gy and the effects on physicochemical factors were measured alongside sensory testing during 3 wk of storage. Significant changes in texture and color with irradiation and age were measured but little visual difference was seen between control and irradiated grapes. However, age had a greater effect on firmness than irradiation for Sugraone grapes. Irradiation did not significantly (P ≤ 0.05) affect the SSC/TA ratio, which increased during storage. The trained panel detected significant changes in the berry texture and rachis color but rated sweetness and flavor significantly higher (P ≤ 0.05) for irradiated Sugraone as compared to the control. Consumers liked both the untreated and 800 Gy treated Sugraone grapes, but liked the untreated grapes more for texture (P ≤ 0.05). However, there was no difference in liking between irradiated (600 Gy or 800 Gy) and control samples of Crimson Seedless for any attribute. The results show that there are varietal differences in response to irradiation but the overall maintenance in quality of irradiated grapes during 3 wk of storage indicates that irradiation can serve as a viable phytosanitary treatment.

Published
2013

Catalog

Books, media, physical & digital resources
See catalog results