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Introduction: Pancreatic Ductal Adenocarcinoma (PDA) is one of the most aggressive malignancies with a 5-year survival rate of 13%. Less than 20% of patients have a resectable tumor at diagnosis due to the lack of distinctive symptoms and reliable biomarkers. PDA is resistant to chemotherapy (CT) and understanding how to gain an anti-tumor effector response following stimulation is, therefore, critical for setting up an effective immunotherapy. Methods: Proliferation, and cytokine release and TCRB repertoire of from PDA patient peripheral T lymphocytes, before and after CT, were analyzed in vitro in response to four tumor-associated antigens (TAA), namely ENO1, FUBP1, GAPDH and K2C8. Transcriptional state of PDA patient PBMC was investigated using RNA-Seq before and after CT. Results: CT increased the number of TAA recognized by T lymphocytes, which positively correlated with patient survival, and high IFN-g production TAAinduced responses were significantly increased after CT. We found that some ENO1-stimulated T cell clonotypes from CT-treated patients were expanded or de-novo induced, and that some clonotypes were reduced or even disappeared after CT. Patients that showed a higher number of effector responses to TAA (high IFN-g/IL-10 ratio) after CT expressed increased fatty acid-related transcriptional signature. Conversely, patients that showed a higher number of regulatory responses to TAA (low IFN-g/IL-10 ratio) after CT significantly expressed an increased IRAK1/IL1R axis-related transcriptional signature. Conclusion: These data suggest that the expression of fatty acid or IRAK1/IL1Rrelated genes predicts T lymphocyte effector or regulatory responses to TAA in patients that undergo CT. These findings are a springboard to set up precision immunotherapies in PDA based on the TAA vaccination in combination with CT. [ABSTRACT FROM AUTHOR]

Raman spectroscopy has been applied to study a range of chromites ([Fe.sup.2+], Mg)(Cr, Al, [Fe.sup.3+])[.sup.2][O.sub.4] with variable chromium to aluminum to iron contents to ascertain conclusions about possible correlations between the chromium concentration and the position of the main Raman peaks within this mineral. Our intention was to examine chromite grains from different paragenesis, which vary significantly in their chromium content, to observe changes in the Raman spectra of this mineral. It was found that a negative correlation exists between the chromium number, calculated from the electron microprobe data, and the Raman peak number. Chromite grains with high chromium numbers show a low Raman peak, whereas samples with low chromium number show a higher Raman peak. Therefore, it is possible to infera relationship between the mineral composition and Raman bands for this type of spinel. The measurements have clearly shown that it is possible to draw precise conclusions about the chromium content of the mineral based on the Raman peak alone. This finding supports a possible application of portable Raman devices on Earth or in space for asteroids and planets., Raman spectroscopy has gained increasing importance in almost all areas of earth and material sciences. This is particularly true in studies of mineralogy, petrology, and volcanology. Raman spectroscopy has also [...]

Vesuvio is likely the most if not one of the most dangerous volcanoes in the world. It is an active volcano, quiescent since 1944. The activity of the Monte Somma and Vesuvio volcanic complex is commonly referred to as two central volcanic edifices, namely Monte Somma and Vesuvio. Nevertheless, the opening of numerous eruptive fissures and related vents have characterized Monte Somma and Vesuvio throughout their lives. Spatter cones, spatter ramparts, and related eruptive fissures are disseminated downslope of Vesuvio's main cone and on the southern slopes of the volcano. Similarly, cinder cones, spatter cones, and welded spatters are distributed in the sequence cropping out on the Monte Somma cliff and on the northern slopes of Monte Somma. In this work, a total of 168 eruptive vents have been identified and characterized in a GIS environment in which field data have been merged with relevant information from historical maps and documents. These vents have been arranged into units bounded by unconformities (Unconformity Bounded Stratigraphic Units) defining the eruptive history of the volcano. Alignments of vents and eruptive fissures within each unit have been compared with regional tectonic elements and the volcano-tectonic features affecting Monte Somma and Vesuvio during the last 5600 years, thus inferring that different structural trends were active in the different stratigraphic units. In particular, we show that the N300°–320° regional, Apennine, left-lateral, strike-slip fault system, the N040°–055° Torre del Greco direct fault system, the N70° and the EW fault system, and the generally NS oriented group of local brittle elements, all analyzed here, were differently active during the investigated time span. These tectonic trends might control the position of the eruptive fissures and vents in case of future unrest of the volcano. [ABSTRACT FROM AUTHOR]

The physical properties of silicate melts are of critical importance for understanding magmatic and volcanic processes on Earth and other planets. Most physical properties of melts are, ultimately, a consequence of the structural organization of the melt. Robust and fully generalizable strategies for the prediction of properties of naturally occurring melts as functions of composition, temperature, and pressure remain a challenging goal. Given the structural origin of macroscopic properties, Raman spectroscopy of glasses, which provides information on melt and glass structure, may provide a useful technique to understanding and quantify variations in macroscopic melt properties. Here, with the aim of providing a generalizable model for predicting the viscosity of silicate melts, we present the results of a Raman spectroscopy campaign performed on 30 anhydrous multicomponent silicate glasses resulting from quenching of remelted and homogenized volcanic rocks and synthetic equivalents. The sample suite comprises one of the largest databases of multicomponent melts for which (a) chemical compositions and (b) physical properties (i.e., viscosity, fragility, heat capacity, and glass transition temperature) are known. Raman spectra have been collected using green light sources at wavelengths of 532 nm. Spectra were collected on the same sample suite in four independent laboratories involving instruments from different manufacturers and, thus, using different spectrometers, detectors, and analytical conditions. Our results are also compared and integrated with published data on some of the same samples derived from two others setups using green light sources with 514.5 and 532 nm wavelegths. For the same sample, the Raman spectra acquired using different setups show different intensities and intensity ratios. However, a strategy based on the ratio between the low‐ and high‐wavenumber peaks (R) was developed to standardize the data to normalized Raman ratios (Rn) and thus to remove interlaboratory differences. Using these advances, we predict melt viscosity solely with the use of Raman spectral measurements of multicomponent silicate glasses, thus demonstrating the potential of the method in describing physical properties of silicate melts. [ABSTRACT FROM AUTHOR]