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The article discusses the evaluation of the RESIST ACINETO immunochromatographic assay for the rapid detection of carbapenemase-producing organisms (CPOs) in positive blood cultures. CPOs, such as Enterobacterales, Pseudomonas aeruginosa, and Acinetobacter baumannii, are challenging resistant pathogens with limited treatment options. The study found that the RESIST ACINETO assay showed positive results for specific carbapenemases in both spiked blood cultures and prospectively collected positive blood cultures. The assay is cost-effective, easy to use, and does not require specialized equipment. However, it is important to note that simultaneous bacterial identification is necessary for accurate results. [Extracted from the article]

Keywords: COVID-19; HIV; CD4; immunocompromised; monoclonal; convalescent plasma EN COVID-19 HIV CD4 immunocompromised monoclonal convalescent plasma 352 355 4 02/07/22 20220201 NES 220201 A late presenter AIDS patient with severe T cell depletion presented non-severe COVID-19 symptoms, with prolonged viral shedding. AIDS patient with severe T cell depletion achieved control but not clearance of SARS-CoV-2 infection PBMNC from the patient and one COVID-19 recovered subject were cultured for 6 h in vitro in medium alone or in presence of peptide pools covering Nucleoprotein, Membrane, and Spike proteins (SARS-CoV-2 antigens). Several cases of fully recovered SARS-CoV-2 infection in patients with genetic or pharmacological B cell depletion for the unrelated condition have been reported, suggesting that a protective immune response against SARS-CoV-2 can be produced even without humoral immunity [16, 17]. [Extracted from the article]

FIM-1 metallo-β-lactamase was previously detected in sporadic Pseudomonas aeruginosa clinical isolates. Here, we report on FIM-1-positive P. aeruginosa from two patients who had shared the same ward in a long-term acute care rehabilitation hospital. Whole-genome sequencing analysis revealed close relatedness of these isolates, which belonged to an ST235 sublineage (clade 8/14) different from those previously reported. Results highlighted the occurrence of clonal diversity among FIM-positive strains and the possibility of their cross-transmission in some healthcare settings., Competing Interests: The authors declare no conflict of interest.

FIM-1 is an acquired metallo-β-lactamase identified in a multidrug-resistant Pseudomonas aeruginosa (index strain FI-14/157) of clinical origin isolated in 2007 in Florence, Italy. Here we report on a second case of infection by FIM-1-positive P. aeruginosa (FI-17645), which occurred in 2020 in the same hospital. Both FIM-1-positive strains exhibited resistance to all anti- Pseudomonas antibiotics except colistin and cefiderocol. Comparative genomic characterization revealed that the two FIM-positive strains were closely related [core genome difference, 16 single nucleotide polymorphisms (SNPs)], suggesting a local circulation of similar strains. In the FI-14/157 index strain, the bla FIM-1 gene was associated with an IS CR19 -like element that likely contributed to its capture downstream an integron platform inserted aboard a Tn 21 -like transposon, named Tn 7703.1 , which was associated with a large integrative and conjugative element (ICE) named ICE 7705 .1, integrated into an att site located within the 3'-end of tRNA Gly CCC gene of the P. aeruginosa chromosome. In strain FI-17645, bla FIM-1 was associated with a closely related ICE, named ICE 7705 . 2 , integrated in the same chromosomal site. Similar ICE platforms, lacking the bla FIM-1 -containing region, were detected in other ST235 P. aeruginosa strains from different geographic areas, suggesting a common ancestry and underscoring the role of these elements in the dissemination of resistance genes in P. aeruginosa . Sequence database mining revealed two draft P. aeruginosa genomes, one from Italy and one from the USA (both isolated in 2012), including a contig with bla FIM-1 , suggesting that this resistance gene could have a broader distribution than originally anticipated., Competing Interests: The authors declare no conflict of interest.