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253 results on '"Girasole M"'

1. Analysis of Amount, Size, Protein Phenotype and Molecular Content of Circulating Extracellular Vesicles Identifies New Biomarkers in Multiple Myeloma

Author

Laurenzana I, Trino S, Lamorte D, Girasole M, Dinarelli S, De Stradis A, Grieco V, Maietti M, Traficante A, Statuto T, Villani O, Musto P, Sgambato A, De Luca L, and Caivano A

Subjects
extracellular vesicles, biomarkers, hematological malignancies, nanoparticle tracking analysis, flow cytometry, digital pcr, Medicine (General), R5-920
Abstract

Ilaria Laurenzana,1,* Stefania Trino,1,* Daniela Lamorte,1 Marco Girasole,2 Simone Dinarelli,2 Angelo De Stradis,3 Vitina Grieco,4 Maddalena Maietti,5 Antonio Traficante,5 Teodora Statuto,4 Oreste Villani,6 Pellegrino Musto,6 Alessandro Sgambato,7 Luciana De Luca,4,* Antonella Caivano4,* 1Laboratory of Preclinical and Translational Research, Centro di Riferimento Oncologico della Basilicata (IRCCS-CROB), Rionero in Vulture, PZ, Italy; 2Institute for the Study of the Structure of Matter, National Research Council (CNR), Rome, Italy; 3Institute for Sustainable Plant Protection, National Research Council (CNR), Bari, Italy; 4Laboratory of Clinical Research and Advanced Diagnostics, Centro di Riferimento Oncologico della Basilicata (IRCCS-CROB), Rionero in Vulture, PZ, Italy; 5Unit of Clinical Pathology, Centro di Riferimento Oncologico della Basilicata (IRCCS-CROB), Rionero in Vulture, PZ, Italy; 6Hematology and Stem Cell Transplantation Unit, Centro di Riferimento Oncologico della Basilicata (IRCCS-CROB), Rionero in Vulture, PZ, Italy; 7Scientific Direction, Centro di Riferimento Oncologico della Basilicata (IRCCS-CROB), Rionero in Vulture, PZ, Italy*These authors contributed equally to this workCorrespondence: Antonella CaivanoLaboratory of Clinical Research and Advanced Diagnostics, IRCCS CROB, Rionero in Vulture, 85028, Potenza, ItalyTel +39 0972 726395Fax +39 0972 726482Email antonella.caivano@crob.itIntroduction: Extracellular vesicles (EVs) are naturally secreted cellular lipid bilayer particles, which carry a selected molecular content. Owing to their systemic availability and their role in tumor pathogenesis, circulating EVs (cEVs) can be a valuable source of new biomarkers useful for tumor diagnosis, prognostication and monitoring. However, a precise approach for isolation and characterization of cEVs as tumor biomarkers, exportable in a clinical setting, has not been conclusively established.Methods: We developed a novel and laboratory-made procedure based on a bench centrifuge step which allows the isolation of serum cEVs suitable for subsequent characterization of their size, amount and phenotype by nanoparticle tracking analysis, microscopy and flow cytometry, and for nucleic acid assessment by digital PCR.Results: Applied to blood from healthy subjects (HSs) and tumor patients, our approach permitted from a small volume of serum (i) the isolation of a great amount of EVs enriched in small vesicles free from protein contaminants; (ii) a suitable and specific cell origin identification of EVs, and (iii) nucleic acid content assessment. In clonal plasma cell malignancy, like multiple myeloma (MM), our approach allowed us to identify specific MM EVs, and to characterize their size, concentration and microRNA content allowing significant discrimination between MM and HSs. Finally, EV associated biomarkers correlated with MM clinical parameters.Conclusion: Overall, our cEV based procedure can play an important role in malignancy biomarker discovery and then in real-time tumor monitoring using minimal invasive samples. From a practical point of view, it is smart (small sample volume), rapid (two hours), easy (no specific expertise required) and requirements are widely available in clinical laboratories.Keywords: extracellular vesicles, biomarkers, hematological malignancies, nanoparticle tracking analysis, flow cytometry, digital PCR

Published
2021

8. Analysis of amount, size, protein phenotype and molecular content of circulating extracellular vesicles identifies new biomarkers in multiple myeloma

Author

Laurenzana, I., Trino, S., Lamorte, D., Girasole, M., Dinarelli, S., De Stradis, A., Grieco, V., Maietti, Maria Emilia, Traficante, A., Statuto, T., Villani, O., Musto, P., Sgambato, Alessandro, De Luca, L., Caivano, A., Maietti M., Sgambato A. (ORCID:0000-0002-9487-4563), Laurenzana, I., Trino, S., Lamorte, D., Girasole, M., Dinarelli, S., De Stradis, A., Grieco, V., Maietti, Maria Emilia, Traficante, A., Statuto, T., Villani, O., Musto, P., Sgambato, Alessandro, De Luca, L., Caivano, A., Maietti M., and Sgambato A. (ORCID:0000-0002-9487-4563)

Abstract

Introduction: Extracellular vesicles (EVs) are naturally secreted cellular lipid bilayer particles, which carry a selected molecular content. Owing to their systemic availability and their role in tumor pathogenesis, circulating EVs (cEVs) can be a valuable source of new biomarkers useful for tumor diagnosis, prognostication and monitoring. However, a precise approach for isolation and characterization of cEVs as tumor biomarkers, exportable in a clinical setting, has not been conclusively established. Methods: We developed a novel and laboratory-made procedure based on a bench centri-fuge step which allows the isolation of serum cEVs suitable for subsequent characterization of their size, amount and phenotype by nanoparticle tracking analysis, microscopy and flow cytometry, and for nucleic acid assessment by digital PCR. Results: Applied to blood from healthy subjects (HSs) and tumor patients, our approach permitted from a small volume of serum (i) the isolation of a great amount of EVs enriched in small vesicles free from protein contaminants; (ii) a suitable and specific cell origin identification of EVs, and (iii) nucleic acid content assessment. In clonal plasma cell malignancy, like multiple myeloma (MM), our approach allowed us to identify specific MM EVs, and to characterize their size, concentration and microRNA content allowing significant discrimination between MM and HSs. Finally, EV associated biomarkers corre-lated with MM clinical parameters. Conclusion: Overall, our cEV based procedure can play an important role in malignancy biomarker discovery and then in real-time tumor monitoring using minimal invasive samples. From a practical point of view, it is smart (small sample volume), rapid (two hours), easy (no specific expertise required) and requirements are widely available in clinical laboratories.

Published
2021

31. Problematiche igieniche relative alla shelf-life del latte pastorizzato

Author

SANTORO, ADRIANO MICHELE LUIGI, SARLI, TERESA ANTONIETTA, CARLINO D, COSTANZO N, FERRANTE S, GIRASOLE M. G, Santoro, ADRIANO MICHELE LUIGI, Carlino, D, Costanzo, N, Ferrante, S, GIRASOLE M., G, and Sarli, TERESA ANTONIETTA

Abstract

Atti XVI Convegno Nazionale A.I.V.I., 22-23-24 giugno 2006, Bari.

Published
2006

35. Servizi di monitoraggio ambientale Campo Naide. Rapporto finale dal pre-survey al IV anno dopo la posa in opera (2004-2009). Misure correntometriche, Indagini sedimentologiche, ecotossicologiche e Analisi di inquinanti in organismi marini

Author

Fabi G., De Ranieri S., Girasole M., Gomiero G., Penna P., and Spagnolo A.

Subjects
piattaforme offshore, valutazione impatto ambientale
Abstract

In riferimento al DEC/VIA/7486 del 22/07/2002 il CNR - ISMAR UOS Ancona è stato incaricato dall'ENI S.p.A. - Divisione E&P di effettuare un monitoraggio volto a valutare il potenziale impatto ambientale conseguente l'installazione della piattaforma Naide e della condotta sottomarina in¬terrata colle¬gante le piattaforme Naide e Cervia C. Nel presente rapporto sono riportati i risultati conseguiti dal pre-survey, condotto nel 2004, a giugno 2009, ovvero nell'intero periodo di indagine.

Published
2014

36. Development and integrated ecological, chemical and biological approach to Evaluate the environmental impact of Mediterranean off shore gas platforms

Author

Gomiero A., Punzo E., De Biasi A., Kozinkova L., Polidori P., Santelli A., Strafella P., Spagnolo A., Girasole M., Dinarelli S., and Negri A***. Viarengo A.

Subjects
Sediments, Monitoring, North-Eastern Mediterranean, Biodiversity, Bio-indicators
Abstract

The pressure for more energy demand is a strong driver for the construction of offshore oil and gas platforms. This also applies to the Adriatic Sea where, since 1960 several gas field were commissioned. The continuous expansion of the gas industry may potentially produce detrimental effects on marine communities, especially in such a peculiar semi-enclosed basin like the Adriatic sea which contains ecosystems that are subject to intensive anthropic pressures, like unsustainable fishing and inputs of environmental pollutants. To date, the environmental effects of gas platforms are poorly understood. Thus, a multidisciplinary approach based on chemical, biological and ecological investigations was performed to evaluate the ecological status as well as the water column and the sediments quality near a gas platforms.

Published
2013

44. Advanced Topics in Cell Model Systems

Author

Macagnano A., Ramundo-Orlando A ., Farrelly F.A., Petri A., Girasole M., and editors

Subjects
Liposome, Permeability change, Gap junction, Channel reconstitution
Published
2009

47. AFM for diagnosis of nanocrystallization of steels in hardening processes

Author

Pompeo G., Girasole M., Longo G., Cricenti A., Bailo D. Ronci F. Maras A., Serracino M., and Moretti P. F.

Subjects
Air shot peening, hardening, martensitic steels, nanocrystalline materials, AFM
Abstract

Introduction: The aim of this study is to investigate the nanocrystallization of steels caused by the transformation from the austenitic to the martensitic phase induced by a severe plastic deformation (SPD) treatment. In this framework, we applied an air blast shot peening treatment, which is a simple protocol widely used for industrial purposes. Methods: AISI 286 and AISI 316 specimens were peened for different times and polished using diamond pastes in order to remove corrugations higher than 1 mu m. The characterization of the steel surfaces was performed by atomic force microscopy (AFM) operating in contact mode. Additional EDXD measurements were performed to confirm the phase transition. Results and Discussion: An AFM-based characterization at nanometric level of the steel surfaces is provided. When the peening exceeds a threshold time that, as expected, depends on the steel composition, a uniform nanostructuration is detected. It is well known that such rearrangement is associated to the growth of a martensitic phase. To date, AFM has been employed in this field only for few applications and to solve specific problems. On the other hand, our results demonstrate that this is a useful technique for the characterization of hardened surfaces, especially when non-destructive sample preparation treatments are required. Moreover, we show that AFM can be a useful tool also for in situ industrial diagnostics of metallic parts.

Published
2008

49. The ETNA consortium

Author

Palomba, E., Moretti, P. F., Maras, Adriana, Girasole, M., Cricenti, A., Longo, G., Pompeo, G., and Somma, F.

Published
2006

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